Structure of the PolIIIα-τc-DNA Complex Suggests an Atomic Model of the Replisome

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Highlights? Crystal structure of the DNA polymerase IIIα in complex with τ_c and a DNA substrate ? τ_c interacts with the C-terminal domain of PolIIIα through its C-terminal helix and the loop that follows it ? PolIIIα in complex with τ_c and DNA displays an open form ? The structure of this complex suggests an atomic model of the bacterial replisome     

Structure of a bacteriophytochrome and light-stimulated protomer swapping with a gene repressor

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Highlights? Bacteriophytochrome structure containing photosensory and output transducing domain ? RpBphP1 binds to the cognate repressor RpPpsR2 on illumination with far-red light ? HOS domain is distantly related to Dhp and is essential for binding to the repressor ? Light-induced protomer swapping mechanism between dimers     

Crystal Structure of the O2-Tolerant Membrane-Bound Hydrogenase 1 from Escherichia coli in Complex with Its Cognate Cytochrome b

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Highlights? The structure of a membrane-bound [NiFe]-hydrogenase/cytochrome b complex ? One active hydrogenase is able to “jumpstart” its O₂-inactivated counterpart ? E. coli hydrogenase 1 has a central protective role against O₂-induced cell damage ? The structure defines the hydrogenase/cytochrome b active-interacting surface     

Architecture of a dodecameric bacterial replicative helicase

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Highlights? Crystal structure of the ATPase domain of DnaB from Helicobacter pylori ? MALLS analysis shows that HpDnaB forms dodecamers ? EM reconstruction shows that HpDnaB forms head-to-head double hexamers     

Structural and functional analysis of HtrA1 and its subdomains

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Highlights? IGFBP-like domain structure suggests that it is incompetent for IGF binding ? Kazal-like domain structure shows distorted “P1” loop incompetent for inhibition ? Protease domain shows catalytic readiness in apo form ? SAXS envelope for full-length HtrA1     

Molecular and structural basis of ESCRT-III recruitment to membranes during archaeal cell division

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Highlights? A conserved protein, CdvA, recruits ESCRT-III to membranes during cell division ? Recruitment is mediated by a peptide-winged helix domain interaction ? We have determined the structure of this complex ? CdvA and a single ESCRT-III protein can drive membrane deformation in vitro     

The bacterial stressosome: a modular system that has been adapted to control secondary messenger signaling

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Highlights? Regulation of cyclic di-GMP biosynthesis in Moorella thermoacetica ? Stressosome signal transduction and resetting ? X-ray crystal structure of type 2C phosphatase MtX ? X-ray crystal structures of phosphorylated MtS and the N-terminal domain of MtR     

Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis

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Highlights? First intimin or invasin β domain structure—both crystallize as monomers ? First structure solved using 3-λ SeMet MAD data from crystals grown in LCP ? Identification of a non-BIG domain directly downstream of the β domain ? Highly conserved and coevolving residues are identified in the Int/Inv family     

The Structure of the Tiam1 PDZ Domain/ Phospho-Syndecan1 Complex Reveals a Ligand Conformation that Modulates Protein Dynamics

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Highlights? The Tiam1 PDZ domain preferentially binds a subset of syndecan (SDC) proteins ? The PDZ domain/phospho-SDC1 structure reveals a phosphoryl binding pocket ? Ligand specificity and phosphorylation regulate PDZ/SDC interactions and signaling ? Tiam1 PDZ domain dynamics are ligand-dependent and regulated by phosphorylation     

Structural and Functional Analysis of the Regulator of G Protein Signaling 2-Gαq Complex

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Highlights? Two structures of the RGS2-Gα_q complex were determined by X-ray crystallography ? RGS2 binds Gα_q in a manner distinct from how other RGS proteins bind Gα_i/o ? In its distinct pose, RGS2 forms extensive contacts with the α-helical domain of Gα_q ? Helical domain contacts contribute to binding affinity and GAP potency of RGS2     

Promiscuous Interactions of gp78 E3 Ligase CUE Domain with Polyubiquitin Chains

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Highlights? The ubiquitin ligase gp78 recruits ubiquitinated substrates via its CUE domain ? gp78CUE:Ub complex reveals a large set of specific interactions ? gp78CUE binds to both distal and proximal moities of diubiquitin in a similar fashion ? The gp78CUE domain binds to K48- and K63-linked diubiquitin equally well     

Mechanism for KRIT1 Release of ICAP1-Mediated Suppression of Integrin Activation

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Highlights? Cocrystal structure of KRIT1 with ICAP1 shows a bidentate interaction interface ? Cocrystal structure of ICAP1 with integrin β1 shows a PTB-peptide interaction ? KRIT1 antagonizes ICAP1-modulated integrin activation by the bidentate interaction ? KRIT1 contains a previously undescribed N-terminal Nudix domain     

A Role for Intersubunit Interactions in Maintaining SAGA Deubiquitinating Module Structure and Activity

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Highlights? Structures of the SAGA deubiquitinating module lacking the Sgf11 zinc finger domain ? The DUB module lacking the Sgf11 zinc finger forms a domain swapped complex dimer ? The conformational changes accompanying domain-swapping disrupt the active site ? Activating mutations in Ubp8 promote the compact form of the DUB module     

Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis

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Highlights? E. histolytica expresses a convergently evolved RGS-RhoGEF protein ? EhRGS-RhoGEF possesses a conventional nine-helix RGS domain ? Activated EhRGS-RhoGEF induces S2 cell spreading through Rac1/2 ? EhRGS-RhoGEF modulates amoebic migration and host cell killing     

Snx3 Regulates Recycling of the Transferrin Receptor and Iron Assimilation

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Highlights? Snx3 is highly expressed in vertebrate hematopoietic tissues ? Silencing of Snx3 results in anemia and hemoglobin defects in vertebrates ? Snx3 and Vps35 physically interact with Tfrc ? Snx3 is required for endosomal recycling of Tf-Tfrc complex     

Structural Mechanism of CCM3 Heterodimerization with GCKIII Kinases

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Highlights? Crystal structure of CCM3-MST4 heterodimeric complex ? Structural mechanism driving CCM3-GCKIII heterodimerization ? Conformational changes required for CCM3-GCKIII heterodimerization ? Synergistic effects of CCM3-MST4 complex on cell proliferation and migration     

HSF-1 regulators DDL-1/2 link insulin-like signaling to heat-shock responses and modulation of longevity

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Highlights? C. elegans DAF-2 insulin-like signaling negatively regulates HSF-1 activity ? DDL-1 and DDL-2 are negative regulators of HSF-1 ? Formation of a complex containing DDL-1/2 and HSF-1 inhibits HSF-1 activity ? DAF-2 signaling promotes DHIC formation by inhibiting DDL-1 phosphorylation     

Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation

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Highlights? The crystal structure of the aa:CP ligase in complex with carrier protein was solved ? The interaction relies on the idiosyncratic α-helix of the aa:CP ligase ? The complex represents a unique mode of carrier protein recognition and binding ? The mechanism of amino acid attachment to carrier protein is discussed     

The n-SET Domain of Set1 Regulates H2B Ubiquitylation-Dependent H3K4 Methylation

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Highlights? H2B ubiquitylation directly stimulates H3K4 methylation by the yeast Set1 complex ? Swd2 is not directly required for the H3K4 methylation activity of the yeast Set1 complex ? The Set1 n-SET domain plays a critical role in H2Bub-dependent H3K4 methylation ? Spp1 is conditionally involved in n-SET- and H2Bub-dependent H3K4 methylation