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1.
The new species Polhillia ignota Boatwr. is described. This species is known only from two collections, one between Vredenburg and Saldanha and the other close to Porterville. The new species is most similar to P. obsoleta, which is known only from a few localities around Worcester, in its narrow, sericeous leaflets and flowers of equal size, but differs in its flattened mature leaves with larger leaflets (up to ± 12 mm long), longer pseudo-peduncles (up to ± 15  mm long), denser inflorescences (with up to four flowers), shorter pedicels (1–2 mm long) and non-auriculate wing- and keel petals.  相似文献   

2.
A new species, Huernia humpatana Bruyns (Apocynaceae–Ceropegieae), closely related to H. similis N.E.Br., is described from the Chela Mountains of Huila Province in southern Angola. The two species are distinguished by the 5-angled and erect stems with more prominent tubercles up to 6 mm long joined into clear angles and separated by V-shaped grooves in H. humpatana as opposed to very obtusely 4-angled stems with tubercles only 2 mm long and only indistinct grooves between the angles in H. similis. Furthermore, in H. similis the nodding corolla is ± 9 mm in diameter with sepals ± 2 mm long, while in H. humpatana the horizontally facing corolla is 18–20 mm in diameter with sepals 4–6 mm long.  相似文献   

3.
The new species, Annesorhiza calcicola Magee and J.C. Manning, is described. It is known only from a few limestone outcrops around Jacobsbaai on the Saldanha Peninsula on the West Coast of South Africa. The species differs from all others in the genus in its small stature (inflorescence less than 0.3 m and leaves less than 150 mm long), the hysteranthous, pilose leaves which are completely absent at flowering (in the other species dead or dying at flowering and usually glabrous), the greyish-black, pilose inflorescence (yellowish-brown and glabrous in the other species) and the smaller homomericarpic fruit less than 5 mm long.  相似文献   

4.
Combretum stylesii O.Maurin, Jordaan & A.E.van Wyk, a liana or slender tree with scrambling branches from the Tugela River Valley (KwaZulu-Natal), South Africa, is described. Molecular data supports its placement in subgenus Combretum section Ciliatipetala with its closest relative Combretum edwardsii. The new species differs from C. edwardsii in having, amongst others, a single mature leaf form, leaves softly textured, with whitish hairs, often with drooping habit, shorter leaf petioles (less than 5 mm), and less complex 8-celled leaf scales. A key to the species and infraspecific taxa of section Ciliatipetala in the Flora of southern Africa region is provided.  相似文献   

5.
A new orchid, Disa albomagentea, is described from the Hottentots Holland Mountains in the Western Cape Province of South Africa. It is an obligately fire-dependent species closely related to Disa obtusa (sect. Disella), from which it can be easily distinguished by the larger flowers, i.e. galea 7-9 mm long versus 4-7 mm long, which are patent and bicoloured, i.e. magenta, white and not spotted versus purple brown, white to cream to greenish, and variously purple spotted. It appears to be endemic to seeps and marshes on the high peaks.  相似文献   

6.
The Kulsi River, a major tributary of Brahmaputra River, N. India is reported to have resident population of Ganges river dolphin, Platanista gangetica (Roxburgh), which feeds on fishes and prawns. While surveying for the dolphins of the river, a number of fishes and prawns were collected. On identification, one of the prawns was found to be undescribed, and hence is described herein. The ecology of the river consisted of: temperature fluctuating widely from 15 to 28 °C, depth from 0.8 to 10 m, turbidity of 11-19 cm, sand mining @ 12,500 MT annually, and fish catch of 300-800 kg (from 1.5 km area). All these factors pose a great threat to the fish and prawn wealth of the river.Macrobrachium kulsiense sp. nov. is a very small sized prawn (maximum size - 34.5 mm in total length), exhibiting species-specific characters, such as highly elevated and moderately long rostrum with 9-12 dorsal teeth, a single ventral tooth, and percentage ratios of ischium, merus, carpus, palm, dactylus of first and second chelipeds (19.05:28.57:33.33:09.52:09.52 and 21.43:25.00:21.43:14.28:17.86, respectively). The species shows close similarity with Macrobrachium mirabile (Kemp). The females are larger than males, eggs are large in size (1.2×0.9 mm) and fecundity is low (15-20).  相似文献   

7.
8.
This investigation addressed faunal relationships with habitat structure within a Zostera marina community targeting differences between seagrass bed edge and interior. Z. marina biomass was significantly higher from the interior portions of the bed compared to the edge, but shoot density did not vary. Additionally, leaf width and length were significantly greater in the interior of the bed, suggesting greater total leaf area. Densities of larger organisms (> 0.85 mm) were significantly greater in vegetated samples (Z. marina edge and interior) compared to unvegetated, but an analysis of similarities demonstrated significant faunal community differences among each of the identified habitats. Densities of small organisms (0.25-0.85 mm), however, were significantly greater at Z. marina edge compared to unvegetated samples and Z. marina interior. Additionally, secondary production (μg AFDW day− 1) was estimated based on the size distribution of taxa and showed significantly greater production from samples gathered in Z. marina compared to unvegetated samples. The relative size distribution of taxa was assessed using regression analysis and results showed that the size distribution was similar for samples collected at edge and interior Z. marina, but these distributions differed significantly when compared to unvegetated samples. The results of this study suggest that although similarities exist between edge and interior portions of Z. marina beds, especially compared to unvegetated habitats, noteworthy differences in faunal density, species composition, size distribution, and secondary production exist between edge and interior Z. marina.  相似文献   

9.
Juvenile or adult Artemia sp. are often used as live prey for the rearing of early life stages of some crustacean, fish and cephalopod species. The improvements of both Artemia growth and its biochemical composition are key issues for the suitable use of Artemia biomass in these rearing processes. In this study we evaluated the growth and survival rates of Artemia fed with the cryptophyte Rhodomonas lens in comparison with different microalgal species commonly used in aquaculture: the prasinophyte Tetraselmis suecica, the prymnesiophyte Isochrysis galbana Parke, and the eustigmatophyte Nannochloropsis gaditana. Microalgae were cultured semi-continuously in nutrient saturated conditions and with a daily renewal rate of 30% of the volume of cultures, to obtain biomass of controlled and optimized composition. Considerable differences in Artemia growth were observed, as well as in the survival rate. At day 8 of rearing, Artemia fed R. lens had the highest length (4.9 ±0.6 mm, P < 0.001), followed by individuals fed T. suecica (4.2 ± 0.7 mm), I. galbana (3.6 ± 0.7 mm) and finally those fed N. gaditana (1.5 ± 0.2 mm). The survival rate of Artemia fed N. gaditana (18 ± 3%) was much lower (P < 0.001) than values found for the remaining groups (69 to 88%). The growth rate of Artemia obtained with R. lens was in general much higher than with other microalgal diets previously reported in the literature. The higher protein content of R. lens could explain the higher growth obtained with this species, but differences of Artemia growth with the different diets could not be explained solely on the basis of the gross composition of microalgae. Factors such as cell size and digestibility all seem to contribute to the results observed. Another trial was carried out to investigate differences in Artemia growth and on its biochemical composition when fed the best two diets: R. lens or T. suecica. The fatty acid (FA) and total amino acid (AA) composition of both microalgal species and the composition of Artemia were assessed as well. As found in the first experiment individuals fed R. lens (group ARHO) grew faster than those fed T. suecica (group ATET), attaining 3.6 ± 0.3 mm and 3.2 ± 0.4 mm (P < 0.001), respectively, after 5 days of rearing. The much higher AA content obtained in R. lens may be on the basis of the higher growth obtained with this species. Protein and carbohydrate levels in Artemia juveniles were very similar in both groups (64-68% of dry weight, and 8-10%, respectively). Lipid was slightly lower in ARHO (12%) than in ATET (15%, P < 0.01). Regarding the FA composition, juveniles from group ARHO contained higher levels of eicosapentaenoic acid (EPA, 6.2%) than juveniles from ATET (4.1%, P < 0.01), whereas docosahexaenoic acid (DHA) was only found in juveniles from ARHO (1.1%). Taking into account that the daily productivity of R. lens culture was higher than, or at least equal, the remaining microalgal species this cryptophyte is confirmed as an excellent diet to optimize the growth of Artemia, as well as to improve its biochemical composition.  相似文献   

10.
A case study on Centaurea gymnocarpa Moris & De Not., a narrow endemic species, was carried out by analyzing its morphological, anatomical, and physiological traits in response to natural habitat stress factors under Mediterranean climate conditions. The results underline that the species is particularly adapted to the environment where it naturally grows. At the plant level, the above-ground/below-ground dry mass (1.73 ± 0.60) shows its investment predominately in the above-ground structure with a resulting total leaf area per plant of 1399 ± 94 cm2. The senescent attached leaves at the base of the plant contribute to limit leaf transpiration by shading soil around the plant. Moreover, the dense C. gymnocarpa leaf pubescence, leaf rolling, the relatively high leaf mass area (LMA = 12.3 ± 1.3 mg cm−2) and leaf tissue density (LTD = 427 ± 44 mg cm−3) contribute to limit leaf transpiration, also postponing leaf death under dry conditions. At the physiological level, a relatively low respiration/photosynthesis ratio (R/PN) in spring results from high R [2.26 ± 0.59 μmol (CO2) m−2 s−1] and PN [12.3 ± 1.5 μmol (CO2) m−2 s−1]. The high photosynthetic nitrogen use efficiency [PNUE = 15.5 ± 0.4 μmol (CO2) g−1 (N) s−1] shows the large amount of nitrogen (N) invested in the photosynthetic machinery of new leaves, associated to a high chlorophyll content (Chl = 35 ± 5 SPAD units). On the contrary, the highest R/PN ratio (1.75 ± 0.19) in summer is due to a significant PN decrease and increase of R in response to drought. The low PNUE [1.5 ± 0.2 μmol (CO2) g−1 (N) s−1] in this season is indicative of a greater N investment in leaf cell walls which may contribute to limit transpiration. On the contrary, the low R/PN ratio (0.05 ± 0.02) in winter is resulting from the limited enzyme activity of the respiratory apparatus [R = 0.23 ± 0.08 μmol (CO2) m−2 s−1] while the low PNUE [3.5 ± 0.2 μmol (CO2) g−1 (N) s−1] suggests that low temperatures additionally limit plant production. The experiment of the imposed water stress confirms that the C. gymnocarpa growth capability is in conformity with the severe conditions of its natural habitat, likewise as it may be the case with others narrow endemic species that have occupied niches with similar extreme conditions.  相似文献   

11.
A new species of microsporidia is described from adults of the thief ant, Solenopsis carolinensis, collected in Florida, USA. Morphological and genetic characterization of this new species showed that it is most closely related to the genus Kneallhazia and is therefore formally designated, Kneallhazia carolinensae sp. nov. Masses of ovoid, binucleate spores were localized to fat body of adult workers and measured 6.2 ± 0.1 × 3.1 ± 0.1 μm (fresh) and 6.0 ± 0.1 × 3.4 ± 0.1 μm (fixed). These spores were in direct contact with the cell cytoplasm and contained an isofilar polar filament with 12-15 coils. Blastn analysis revealed that the K. carolinensae 16S rDNA sequence exhibited 91% identity with the 16S rDNA gene of K. solenopsae. The morphological and sequence data support the conclusion that K. carolinensae is a novel microsporidian species distinct from K. solenopsae.  相似文献   

12.
Morphological, anatomical and physiological summer and winter leaf traits of Cistus incanus subsp. incanus, C. salvifolius and C. monspeliensis growing at the Botanical garden of Rome were analyzed. With regard to differences between summer and winter leaves of the considered species, leaf thickness (L) was 21% higher in summer than in winter leaves (mean of the considered species) and this increase was mostly the result of the increased palisade parenchyma thickness over the spongy parenchyma one (24 and 16% higher in summer than in winter leaves, respectively). Leaf mass area (LMA) and leaf tissue density (LTD) were 38% and 17% higher in summer than in winter leaves, respectively (mean of the considered species). The photosynthetic rate (PN), stomatal conductance (gs) and chlorophyll content (Chl) of summer leaves were 54%, 17% and 14% lower, respectively, than in winter leaves. C. monspeliensis summer leaves had the highest LMA, LTD, adaxial cuticle thickness (14.6 ± 1.8 mg cm−2, 1091 ± 94 mg cm−3, and 5.8 ± 1.7 μm, respectively) and the lowest mesophyll intercellular spaces (fias 38 ± 3%). Moreover, C. monspeliensis had the highest PN in summer (2.6 ± 0.1 μmol m−2 s−1) and C. incanus the highest PN and WUE (84% and 59% higher than the other species) in the favorable period, associated to a higher fias (42 ± 2%). C. salvifolius had the highest PN (54% higher than the other species) in winter. The plasticity index could allow a better interpretation of the habitat preference of the considered species. The physiological plasticity (PIp = 0.39, mean value of the considered species) was higher than the morphological (PIm = 0.22, mean value) and anatomical (PIa = 0.13, mean value) plasticity. Moreover, among the considered species, C. salvifolius and C. incanus are characterized by a larger PIa (0.14, mean value) which seems to be correlated with their wider ecological distribution and the more favorable conditions of the environments where they naturally occur. The highest PIm (0.29) of C. monspeliensis indicates that it can play a high adaptive role in highly stressed environments, like fire degraded Mediterranean areas in which it occurs.  相似文献   

13.
This study was aimed at evaluating the antifungal, acetylcholinesterase inhibition and antioxidant activities of petroleum ether, dichloromethane, ethanol and methanol extracts from different parts of Barleria prionitis, Barleria greenii and Barleria albostellata. Their phytochemical properties and the possibility of plant-part substitution as a conservation strategy against destructive harvesting (use of aerial parts and roots) of these species for medicinal purposes were also investigated. Microtitre plate assays were used in determining their antifungal (against Candida albicans) and acetylcholinesterase inhibition activities. All the extracts demonstrated both fungistatic and fungicidal activities with the minimum inhibitory concentration ranging from 0.78 to 9.38 mg/ml and minimum fungicidal concentration ranging from 1.17 to 12.50 mg/ml. The higher inhibitory activity of B. greenii leaf extracts in most cases compared to similar extracts of the stems and roots suggest the potential of B. greenii leaves in plant-part substitution. At the lowest extract concentration (0.156 mg/ml), the leaf extract of B. greenii demonstrated a significantly higher acetylcholinesterase (AChE) inhibition than its stem and root extracts. In B. albostellata, the AChE inhibitory activity demonstrated by the stem was significantly greater than that recorded in its leaf extract. These findings suggest that the idea of plant part substitution may be species and/or biological activity dependent. In the DPPH radical scavenging assay, different parts of Barleria species showed free radical scavenging activity with EC50 values ranging from 6.65 to 12.56 μg/ml. The ability of the extracts from different plant parts to reduce ferric ion/ferricyanide complex to the ferrous form and decrease carotenoid bleaching rate suggests the presence of antioxidant compounds capable of donating electrons and hydrogen atoms in their reaction mechanisms. Flavonoids, iridoids and tannins were detected in the different parts of these Barleria species. These phytochemicals might be responsible for the observed biological activities. The isolation of specific bioactive compounds through bioassay-guided fractionation and their characterization as well as studies evaluating their safety may be necessary in the exploration of these species for potential new therapeutic drugs or drug leads.  相似文献   

14.
This paper introduces a multi-cylinder evapotranspirometer method, which can directly measure evapotranspiration (ET) from emergent plants in different species and states as well as simultaneously measure evaporation (EW) from an open water surface. Values of daily ET from three contrasting reed (Phragmites australis) stands, with different leaf area indexes (LAI), were obtained through in situ measurements of the Baiyangdian wetland using this method during the growing seasons in 2008 and 2009. The results showed that the ET rate of the reed belt was very high, even exceeding 20 mm d−1 under extreme weather conditions. Depending on the LAI change, the annual ET from the different reed canopies ranged from 970 to 2035 mm, whereas the ET/EW ratios ranged from 2.05 to 3.98. Accuracy analysis results showed that the errors of the measurement from this method were no more than 2 mm. The relative errors of the measurement were correspondingly from 0.04% to 0.33%. It is indicated that the accuracy of our measurement is good enough for the requirements of the ET measurement.  相似文献   

15.
Genomes of opisthorchid species are characterized by small size, suggesting a reduced amount of repetitive DNA in their genomes. Distribution of repetitive DNA sequences in the chromosomes of five species of the family Opisthorchiidae (Opisthorchis felineus 2n = 14 (Rivolta, 1884), Opisthorchis viverrini 2n = 12 (Poirier, 1886), Metorchis xanthosomus 2n = 14 (Creplin, 1846), Metorchis bilis 2n = 14 (Braun, 1890), Clonorchis sinensis 2n = 14 (Cobbold, 1875)) was studied with C- and AgNOR-banding, generation of microdissected DNA probes from individual chromosomes and fluorescent in situ hybridization on mitotic and meiotic chromosomes. Small-sized C-bands were discovered in pericentric regions of chromosomes. Ag-NOR staining of opisthorchid chromosomes and FISH with ribosomal DNA probe showed that karyotypes of all studied species were characterized by the only nucleolus organizer region in one of small chromosomes. The generation of DNA probes from chromosomes 1 and 2 of O. felineus and M. xanthosomus was performed with chromosome microdissection followed by DOP-PCR. FISH of obtained microdissected DNA probes on chromosomes of these species revealed chromosome specific DNA repeats in pericentric C-bands. It was also shown that microdissected DNA probes generated from chromosomes could be used as the Whole Chromosome Painting Probes without suppression of repetitive DNA hybridization. Chromosome painting using microdissected chromosome specific DNA probes showed the overall repeat distribution in opisthorchid chromosomes.  相似文献   

16.
The trophozoites of a novel gregarine apicomplexan, Trichotokara nothriae n. gen. et sp., were isolated and characterized from the intestines of the onuphid tubeworm Nothria conchylega (Polychaeta), collected at 20 m depth from the North-eastern Pacific Coast. The trophozoites were 50-155 μm long with a mid-cell indentation that formed two prominent bulges (anterior bulge, 14-48 μm wide; posterior bulge, 15-55 μm wide). Scanning electron microscopy (SEM) demonstrated that approximately 400 densely packed, longitudinal epicytic folds (5 folds/μm) inscribe the surface of the trophozoites, and a prominently elongated mucron (14-60 μm long and 6-12 μm wide) was covered with hair-like projections (mean length, 1.97 μm; mean width, 0.2 μm at the base). Although a septum occurred at the junction between the cell proper and the mucron in most trophozoites, light microscopy (LM) demonstrated that the cell proper extended into the core of the mucron as a thin prolongation. A spherical nucleus (8-20 μm) was situated in the middle of the trophozoites, and gamonts underwent caudal syzygy. The small subunit (SSU) rDNA sequence and molecular phylogenetic position of T. nothriae was also characterized. The sequence from this species was the most divergent of all SSU rDNA sequences currently known from gregarines and formed a weakly supported clade with Lecudina polymorpha, which also possesses densely packed epicyctic folds (3-5 folds/μm) and a prominently elongated mucron.  相似文献   

17.
The taxonomy of the order Zoantharia (=Zoanthidea=Zoanthiniaria) is greatly hampered by the paucity of diagnostic morphological features. To facilitate discrimination between similar zoanthids, a combination of morphological and molecular analyses is applied here. The three most abundant zoanthid species in shallow waters of the southern Chilean fjord region are described. Comparison with other zoanthids using molecular markers reveals that two of them are new to science; these are described as Mesozoanthus fossii gen. n., sp. n. and Epizoanthus fiordicus sp. n. Their representatives grow on rocky substratum and do not live in symbiosis with demosponges. In the less abundant M. fossii, animals are greyish in colour and resemble members of Parazoanthus in growth form. Individual polyps can be up to 35 mm long. The more abundant E. fiordicus are also greyish; the polyps arise from thin stolons and reach only 12 mm in length. The third species studied is Parazoanthus elongatus McMurrich, 1904. For these three Chilean zoanthid species, in-situ photographs are presented as well as information on distribution, habitat and associated species. Establishment of the Mesozoanthus gen. n. is of particular importance to taxonomy in the chaotic suborder Macrocnemina.  相似文献   

18.
Glaucium flavum Crantz. is found in an anthropized coastal grassland at the joint estuary of the Tinto and Odiel rivers (SW Spain), growing under the influence of high levels of copper contamination derived from nearby petrochemical industries, with no obvious adverse affects on the performance of the plant. In addition, this species exhibits a series of ecological characteristics which may render it appropriate for use in the phytoremediation of contaminated areas. Nonetheless, the response of G. flavum to elevated copper concentrations has not been studied. A greenhouse experiment was conducted to investigate the effects of a range of Cu concentrations (0 to 47 mmol l−1) on the growth, reproduction and photosynthetic performance of G. flavum, by measuring relative growth rate, fruit and seed production, chlorophyll fluorescence parameters, gas exchange and photosynthetic pigment concentrations. We also determined total copper, nitrogen, phosphorous, sulphur, calcium and magnesium concentrations. G. flavum survived with concentrations of up to 730 mg Cu kg−1 DW in the leaves, when treated with 30 mmol Cu l−1 (2000 mg l−1). Quantum efficiency of PSII, net photosynthesis rate, as well as leaf Ca and Mg concentrations were all negatively affected by Cu concentrations greater than 9 mmol l−1 in the nutrient solution. Our results indicate that the reduction in photosynthetic performance may be attributed to the adverse effect of excess Cu on the photosynthetic apparatus of the plant, both directly, via a decrease in pigment concentrations, and indirectly, via interference of Cu with Ca ions of PSII. Growth and seed production were only slightly affected by leaf tissue concentrations as high as 230 mg Cu kg−1 dry mass, which suggests that this species could play an important role in phytoremediation of Cu-contaminated soils.  相似文献   

19.
In a 4-week study, we investigated the effects of increasing soil NaCl (100–400 mM) on photosynthesis, salt uptake and transport, and intracellular compartmentation of Na+ and Cl in 1-year-old seedlings of Kandelia candel (L.) Druce and Bruguiera gymnorhiza (L.) Savigny. Increasing NaCl stress significantly elevated Na+ and Cl in root and shoot tissues (stem + leaf) of both species, but B. gymnorhiza showed a rapid Na+ accumulation upon the initiation of salt stress and leaves contained 90% more Na+ and 40% more Cl than K. candel at the end of experiment. Net photosynthetic rate (Pn) declined with increasing salinity, and the most marked reduction occurred after exposure of mangrove seedlings to a severe salinity, 400 mM NaCl. However, the inhibitory effects of severe stress varied with species: Pn decreased by 80% in K. candel whereas in B. gymnorhiza the decline was 60%. The quantum yield (AQY) and carboxylation efficiency (CE) response to severe salinity showed a trend similar to Pn, in which a lesser reduction of AQY and CE was observed in B. gymnorhiza (33–35%), as compared to K. candel (43–52%). X-ray microanalysis of leaf mesophyll cells showed evidence of distinct vacuolar compartmentation of Na+ in K. candel but Cl in B. gymnorhiza after seedlings were subjected to 100 mM NaCl for 7 d. Moreover, Na+ within cell wall, cytoplasm, vacuole and chloroplast remained 23–72% lower in stressed B. gymnorhiza as compared to K. candel. In conclusion, B. gymnorhiza exhibited effective salt exclusion from chloroplasts although increasing salt stress caused a rapid and higher build up of Na+ and Cl in the leaves. We suggest that the salt-induced Pn reduction in the two mangrove species is correlated with the ability to exclude Na+ and Cl from the chloroplast, rather than with the bulk leaf salt concentration.  相似文献   

20.
In this study we investigated above-ground biomass and morphological responses of a floating-leaved plant species, Nymphaea alba, to small spring water level manipulations (0.1–0.5 m) in a large, shallow lake over a 9-year period (1995–2003). A year effect was found in mean annual above-ground plant biomass with higher values found in years of low water levels, 275–339 g DW m−2 in 1995 and 2003 against 143–198 g DW m−2 in 1996–2002 (no data transformation). No significant changes in biomass patterns were observed within each season (one summer peak), except in 1995 when a summer decline in biomass occurred. The amplitude and duration of exposure to high water levels affected the spring and annual above ground biomass of N. alba. The plant responded to high spring water levels by producing longer and thinner petioles to preserve leaves from flooding while no significant changes in leaf surface area (except in May) and leaf/petiole biomass ratio were obtained. The results are interpreted with regard to plant adaptations to changing environments (biomass allocation patterns in the different plant organs and stem density) and the effects of other abiotic factors relevant to the size of the system. We concluded that small deviations in spring water level can be driving forces in a large system in controlling the above-ground biomass of this floating-leaved plant.  相似文献   

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