A description of the first intact Dwarf Sperm whale from the South China Sea and a review of documented specimens of the Kogiidae (Cetacea) from Hong Kong

Reports of the Dwarf Sperm whale (Kogia sima) in Chinese waters exist only for four specimens, which stranded along the coast of the East China Sea. Records of this species in the South China Sea consist of one skull noted as present in a Vietnamese ‘whale temple’. Recently recognized material in the biological sciences building of the University of Hong Kong, confirms the presence of both the Dwarf (K. sima) and the Pygmy (K. breviceps) Sperm whales in Hong Kong. In addition, close examination of Hong Kong stranding records reveals that of the four specimens identified as K. breviceps one is, in fact, K. sima. A comparison of the Hong Kong material, the holotype of K. sima and other Asian specimens of Kogia spp. affirms that most of the features already used elsewhere to distinguish the two species are reliable.     

Eating Metropolitaneity: Hong Kong Identity in yumcha

In Hong Kong, yumcha as a pervasive form of eating in the public has become an institution which epitomises the diversity and inclusiveness that Hong Kong people think are quintessential to the civility and economic achievement of the metropolis. It stands out particularly as a practice that has come to represent both locally and overseas a Hong Kong culture which has previously been characterised as residual of ‘Chinese tradition’ and ‘Western customs’, and hence is virtually non-existent. This paper looks at the consumption of metropolitaneity and the culturalisation of the heunggongyan or Hong Kong personal identity in the form, content and social relations involved in yumcha. It points to the significance of yumcha as a key to understanding a collective identity which has become more and more apparent as Hong Kong people vehemently try to establish a city with its own culture, first against the colonial grip and then the return of sovereignty to China in 1997.     

Distribution of toxigenic Bacillus cereus in rice samples marketed in Hong Kong

Eleven of 16 samples of rice on sale in rice shops and supermarkets in Hong Kong contained Bacillus cereus. Although B. cereus counts did not exceed 100 bacteria/g in most of the positive samples, a sample of Thai red rice and a poor quality rice originating from China contained between 300 to 1000 cells/g and 10⁴ to 2×10⁵ cells/g, respectively. Nine strains produced an enterotoxin responsible for the diarrhoeal-type B. cereus food poisoning and seven of these strains also produced a haemolysin (haemolysin BL), a dermonecrotic vascular permeability factor which may be a virulence determinant in diarrhoeal illness caused by this bacterium.P.K. Lee and J.A. Buswell are with the Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; K. Shinagawa is with the Department of Veterinary Medicine, Iwate University, Iwate, Japan.     

Purification and properties of β-amylase from Bacillus circulans S31

A thermotolerant -amylase was purified from Bacillus circulans S31 isolated from soil in Hong Kong. The purified enzyme has an M _r of 64 kDa and was stable at 50°C and pH 7.0 for 30 min. Its K _m for starch was 0.9 mg/ml with a V _max of 0.3 mg/min. It was not activated by any metal ion although sulphydrys reagents were inhibitory.H.S. Kwan, K.H. So and K.Y. Chan are with the Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong S.C. Cheng is with the Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic, Hung Hom, Hong Kong.     

Book reviews

Dinosaurs from China

DONG ZHIMING. China Ocean Press, Hong Kong, 1987. vi + 114 pp. ISBN 7–5027–0116–8. Hardcover ed., approximately $34.00; softcover ed., approximately $24.00 [This is the first English edition.]

DONG ZHIMING, with text by Angela C. Milner. China Ocean Press, Hong Kong, and the British Museum (Natural History), London, 1988. vi +114 pp. ISBN 0565–01073–5. Softcover ed., £9.95 at the Natural History Museum bookstore. [A hardcover edition probably exists, but I have not seen it.]

TRACE FOSSILS: BIOLOGY AND TAPHONOMY, by Richard G. Bromley, Unwin Hyman, London 1990. 280 pp. ISBNO 04 4453035. £40 hardback, £17.95 softback.

EXTINCTION EVENTS IN EARTH HISTORY, Editors E.G. Kauffman and O.H. Walliser. Springer‐Verlag, Berlin 1990. 431 pp. ISBN 0–387–52605–6. Price DM 75.

PHYLOGENY AND THE CLASSIFICATION OF FOSSIL AND RECENT ORGANISMS, edited by Norbert Schmidt‐Kittler and Rainer Willmann. Abhandlungen des Naturwissenschaftlichen Vereins in Hamburg, N.P. 28, Verlag Paul Parey, Hamburg & Berlin ISBN 3–490–14496–1.     

Vermicomposting in the management of pig-waste in Hong Kong

The treatment and disposal of pig-waste in Hong Kong has received much attention in recent years but, following any of the presently used treatment processes, solids remain to be further stabilized. Vermicomposting is a waste stabilization technique which converts waste into potentially recyclable materials such as worm protein and worm casts. The earthworm, Pheretima asiatica, can stabilize most of the solids arising from the treatment of pig-waste, including raw pig manure, suggesting that vermicomposting has a high potential as a unit process in the management of pig-waste in Hong Kong.S.H. Wong is with the Environmental Protection Department, Hong Kong; and D.A. Griffiths is with the Department of Botany, University of Hong Kong, Hong Kong.     

Lignocellulolytic enzyme profiles of edible mushroom fungi

One of the most economically-viable processes for the bioconversion of many types of lignocellulosic wastes is represented by edible mushroom cultivation. Lentinula edodes, Volvariella volvacea and Pleurotus sajor-caju are three important commercially cultivated mushrooms which exhibit varying abilities to utilise different lignocellulosics as growth substrate. Examination of the lignocellulolytic enzyme profiles of the three species show this diversity to be reflected in qualitative variations in the major enzymic determinants (i.e. cellulases, ligninases) required for substrate bioconversion. For example, L. edodes, which is cultivated on highly lignified substrates such as wood or sawdust, produces two extracellular enzymes which have been associated with lignin depolymerisation in other fungi, (manganese peroxidase and laccase). Conversely, V. volvacea, which prefers high cellulose-, low lignin-containing substrates produces a family of cellulolytic enzymes including at least five endoglucanases, five cellobiohydrolases and two -glucosidases, but none of the recognised lignin-degrading enzymes.J.A. Buswell, Y.J. Cai, S.T. Chang, J.F. Peberdy and S.Y. Fu are with the Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong. J.A. Buswell and S.T. Chang are also with the Centre for International Services to Mushroom Biotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong. J.F. Peberdy is also with the Department of Life Science, University of Nottingham, United Kingdom. S.Y. Fu is also with, and H.-s. Yu is with the Institute of Chemistry, Academia Sinica, Guangzhou, China.     

Hong Kong: The Anthropology of a Chinese Metropolis

Hong Kong: The Anthropology of. Chinese Metropolis. Grant Evans and Maria Tarn Siu-Mi. eds. Honolulu: University of Hawaii Press, 1997.336 pp.     

Isolation and characterization of restriction endonuclease EclHKI from an Enterobacter cloacae strain

An Enterobacter cloacae strain, producing restriction enzyme EclHKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam1105I, which recognizes and cleaves GACNNN!NNGTC. EclHKI was produced at high activity (4×10⁴ U/g wet cells) and was purified from contaminants which interfere with restriction digestion by passing the cell lysate through DEAE-Sephacel and heparin columns. Activity was optimal at 37°C in a medium salt buffer.H.-Y. Chan, Y.-C. Chan and P.-C. Shaw are with the Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong: K.-M. Kam is with the Institute of Pathology, Sai Ying Pun Jockey Club Clinic, Hong Kong.     

Nutrient flow in a major urban settlement: Hong Kong

A nutrient balance is established for the contemporary urban ecosystem of Hong Kong. The flow of nutrients in the Hong Kong food system in particular is examined, including current and potential nutrient recycling patterns. Losses of nutrients in food for human consumption are found to be up to 20% for major nutrients. The flow of mineral phosphorus in the Hong Kong food system is examined in detail. About 3600 tonnes of phosphorus are lost from the Hong Kong food system each year. A comparison is made between the land-based forage area demand of the Hong Kong population and a similar-sized Western population, that of Sydney, Australia. It is estimated that the average Hong Kong person consumes a diet which requires only half the land area needed to produce the diet of the average Sydney person. However, Hong Kong relies on the ocean for 25% of its animal protein supply compared with 2.5% for Sydney. Patterns of food production and nutrient recycling are proposed, with the aim of optimizing resource utilization in close association with contemporary urban settlements.     

Degradation of tannins in spent coffee grounds by Pleurotus sajor-caju

Pleurotus sajor-caju PL27, a white rot fungus, degraded up to 87% of the tannins in spent coffee grounds as a solid substrate over 32 days. Degradation of tannins was enhanced if potato and dextrose were included. The potential nutritive value of the substrate as animal feed may be improved by this process.Yum-Shing Wong is with the Biology Department, Chinese University of Hong Kong, Shatin, NT, Hong Kong. Xun Wang is a visiting scientist from the Research Institute of Food and Fermentation Industry, People's Republic of China.     

Clara Law's Floating Life and Hong Kong-Australian 'flexible citizenship'

Clara Law's film Floating Life was the first Australian film to be nominated for an Oscar for Best Foreign Film, and the first Australian film to deal with migrant Hong Kong Chinese identities 'from inside'. From perspectives of transnational Chinese migration and flexible citizenship, this article looks at Floating Life as a Hong Kong Chinese migrant reading of Australia, which defamiliarizes and recontextualizes familiar Australian localities and geopolitical formations, contrasting them with the film's other principal loci of Hong Kong and Germany. It also interprets the film as a neo-Confucian study of family disintegration in a migrant context, and an exploration of notions of home and identity.     

Village Life in Hong Kong: Politics, Gender, and Ritual in the New Territories

Village Life in Hong Kong: Politics, Gender, and Ritual in the New Territories . James L. Watson and Rubie S. Watson. Sha Tin, NT: Chinese University Press, 2004. 490 pp.     

A broader view of conservation

A review of Conservation in a Changing World, edited by Georgina M. Mace, Andrew Balmford, and Joshua R. Ginsburg. Cambridge, Cambridge University Press, 1998, 308 pp. $80.00 hard bound; $34.95 paperback.     

MSH2 c.1452-1455delAATG is a founder mutation and an important cause of hereditary nonpolyposis colorectal cancer in the southern Chinese population

Hereditary nonpolyposis colorectal cancer (HNPCC) accounts for approximately 2% of all colorectal cancer (CRC) cases and is the most common hereditary CRC syndrome. We have previously reported a high incidence of microsatellite instability (MSI) and germline mismatch repair (MMR) gene mutations in young Hong Kong Chinese with CRC. Ongoing studies at the Hereditary Gastrointestinal Cancer Registry in Hong Kong have revealed a unique germline MSH2 c.1452-1455delAATG mutation that has not been reported in other ethnic groups. Detailed analysis showed that this specific MSH2 mutation constituted 21% of all germline MMR gene mutations and 36% of all MSH2 germline mutations identified. We designed a specific PCR-based diagnostic test on paraffin-embedded tissues and identified this germline mutation in 2 (1.5%) of 138 consecutive patients with early-onset CRC (<46 years of age at diagnosis). Haplotype analysis was performed using 11 microsatellite markers located between D2S391 and D2S123. All 10 families had the same disease haplotype, suggesting a founder effect. These 10 families all originated from the Chinese province of Guangdong, which historically included Hong Kong. It is the most populous of the Chinese provinces, with a population of >93 million. Further analysis suggested that this founder mutation may date back to between 22 and 103 generations ago. The identification of this MSH2 founder mutation has important implications for the design of mutation-detection strategies for the southern Chinese population. Since there were major emigrations from Hong Kong and Guangdong province during the 19th and 20th centuries, this finding is also significant for Chinese communities worldwide.     

A possible geographic origin of endemic hepatitis C virus 6a in Hong Kong: evidences for the association with Vietnamese immigration

Background

Hepatitis C virus (HCV) 6a accounts for 23.6% of all HCV infections of the general population and 58.5% of intravenous drug users in Hong Kong. However, the geographical origin of this highly predominant HCV subgenotype is largely unknown. This study explores a hypothesis for one possible transmission route of HCV 6a to Hong Kong.

Methods

NS5A sequences derived from 26 HCV 6a samples were chosen from a five year period (1999–2004) from epidemiologically unrelated patients from Hong Kong. Partial-NS5A sequences (513-bp from nt 6728 to 7240) were adopted for Bayesian coalescent analysis to reconstruct the evolutionary history of HCV infections in Hong Kong using the BEAST v1.3 program. A rooted phylogenetic tree was drawn for these sequences by alignment with reference Vietnamese sequences. Demographic data were accessed from “The Statistic Yearbooks of Hong Kong”.

Results

Bayesian coalescent analysis showed that the rapid increase in 6a infections, which had increased more than 90-fold in Hong Kong from 1986 to 1994 correlated to two peaks of Vietnamese immigration to Hong Kong from 1978 to 1997. The second peak, which occurred from 1987 through 1997, overlapped with the rapid increase of HCV 6a occurrence in Hong Kong. Phylogenetic analyses have further revealed that HCV 6a strains from Vietnam may be ancestral to Hong Kong counterparts.

Conclusions

The high predominance of HCV 6a infections in Hong Kong was possibly associated with Vietnamese immigration during 1987–1997.     

Molecular Coordinated Regulation of Gene Expression During Ovarian Development in the Penaeid Shrimp

To understand the molecular events of ovarian development in penaeid shrimp, RNA arbitrarily primed polymerase chain reaction (RAP-PCR) was used to identify differentially expressed genes during ovarian maturation in Metapenaeus ensis. From a screening of 700 clones in a cDNA library of the shrimp ovary by the products of RAP-PCR of different maturation stages, 91 fragments with differentially expressed pattern as revealed by dot-blot hybridization were isolated and sequenced. Forty-two of these fragments show significant sequence similarity to known gene products and the differentially expressed pattern of 10 putative genes were further characterized via Northern hybridization. Putative glyceraldehyde–3–phosphate dehydrogenase and arginine kinase are related to provision of energy for active cellular function in oocyte development. Translationally controlled tumor protein, actin, and keratin are related to the organization of cytoskeleton to accomplish growth and development of oocytes. High mobility group protein DSP1, heat shock protein 70, and nucleoside diphosphate kinase may act as repressors before the onset of ovarian maturation. Peptidyl-prolyl cis-trans isomerase and glutathione peroxidase are related to the stabilization of proteins and oocytes. This study provides new insights on the molecular events in the ovarian development in the shrimp. Present addresses: T.S. Lo, Department of Applied Science, Hong Kong Institute of Vocational Education, Chai Wan, Hong Kong, China J.L.Y. Mong, Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China Q.W.L. Wong, Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Shatin, Hong Kong, China     

Explaining Chinese identification in Hong Kong: the role of beliefs about group malleability

ABSTRACT

Hong Kong is an epicentre of identity conflicts in China. While numerous accounts have been proposed to explain the rise of “localism”, few have explained why many Hong Kong Chinese have maintained their ethnic identification with China. Based on an original survey of a representative sample of the population and an experiment conducted among college students, this article advances a novel explanation, arguing that people who perceive the core character of groups as shaped by context (i.e. it is malleable) are more likely to have a higher level of Chinese identification. The explanation is that in the face of deep cultural and political differences between mainland and Hong Kong Chinese, the belief induces the latter to understand those differences as stemming from people’s current situations rather than from some unchangeable national traits, thus reducing their need to adopt an alternative identity. The findings have important implications on (ethno) nation-building policies.     

The Evolution of Endocrine Systems in Vertebrates

A review of Comparative Vertebrate Endocrinology, 3rd edition, by P.J. Bentley. Cambridge: Cambridge University Press, 1998, 526 pp, $100.00, hardbound; $44.95, paperback.     

The Collembolan,oudemansia Esakii,an Intertidal Marine Insect in Hong Kong,with Emphasis on the Floatation Method of Collection

Summary Oudemansia esakii is shown to be a truly marine insect as all stages of the life cycle are found in intertidal sand in Hong Kong. The motile stages migrate vertically and forage during low tide, but burrow into the sand with incoming tidal conditions. An air bubble often forms around them while in the soil. Total forced immersion for 75 hours showed no harmful effects. The greatest burrowing depth is 36 cm and the optimal littoral position is three feet above low water. A floatation method using graded sieves is superior to MacFadyen's high gradient technique. Pertinent hydrographic data are included, as are biological relationships.Marine Science Laboratory, the Chinese University of Hong Kong