共查询到20条相似文献,搜索用时 15 毫秒
1.
Resonance Rayleigh scattering method for the determination of chitosan with some anionic surfactants
In weak acidic buffer medium, chitosan binding with an anionic surfactant, such as sodium dodecyl benzene sulphonate (SDBS), sodium lauryl sulphate (SLS) or sodium dodecyl sulphonate (SDS), can result in a significant enhancement of resonance Rayleigh scattering (RRS) intensity. The results showed that under optimum conditions the enhanced RRS intensity is proportional to the concentration of chitosan in the range 0.10–20.0 µg/mL for SDBS, 0.27–15.0 µg/mL for SLS and 0.20–15.0 µg/mL for SDS. Among these, the sensitivity of SDBS is the highest and its detection limit for chitosan is 29 ng/mL, while those of SLS and SDS are 83 and 61 ng/mL, respectively. The method has good selectivity and was applied to the determination of trace amounts of chitosan in practical samples with satisfactory results. Therefore, a simple and convenient method with high sensitivity and selectivity for the determination of chitosan was established. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
2.
The present investigation aims at studying the effect of mixed surfactant system of sodium lauryl sulphate (SLS) and alkyl
polyglucosides (C10APG, C12APG and C12/14APG) on dissolution rate enhancement of poorly water soluble drug. Aceclofenac—a non-steroidal anti-inflammatory agent was
used as a model drug as it has limited water solubility. The influence of the surfactant concentration in various blends on
dissolution rate of Solid Dispersion (SD), prepared using solution method with ethanol as the solvent was studied and the
advantage of mixed surfactant systems over the individual surfactants was illustrated by differences in the in-vitro dissolution profiles of SD. Physico chemical evaluation (critical micellar concentration, zeta potential and β-parameter
calculations) was carried out to study the mixed surfactant systems. Solid mixtures were characterized by Infrared spectroscopy
(FT-IR); X-ray diffraction studies (XRD) and scanning electron microscopy (SEM). It was seen that the dissolution rate of
aceclofenac from SD increased with the increase in the APG proportion relative to SLS with the optimum ratio of 0.2 SLS:0.8
APG showing the best effect in all cases. Results obtained from physico-chemical evaluation (the decrease in the value of
critical micelle concentration and higher negative value of β-parameters) suggested the existence of synergism between surfactants
blends. The observed results in the dissolution rate enhancement could be attributed to the drug—surfactant interactions as
evident from FT-IR, SEM and XRD results. 相似文献
3.
Forming a salt is a common way to increase the solubility of a poorly soluble compound. However, the solubility enhancement
gained by salt formation may be lost due to solution-mediated phase transformation (SMPT) during dissolution. The SMPT of
a salt can occur due to a supersaturated solution near the dissolving surface caused by pH or other solution conditions. In
addition to changes in pH, surfactants are also known to affect SMPT. In this study, SMPT of a highly soluble salt, haloperidol
mesylate, at pH 7 in the presence of a commonly used surfactant, sodium lauryl sulfate (SLS), was investigated. Dissolution
experiments were performed using a flow-through dissolution apparatus with solutions containing various concentrations of
SLS. Compacts of haloperidol mesylate were observed during dissolution in the flow-through apparatus using a stereomicroscope.
Raman microscopy was used to characterize solids. The dissolution of haloperidol mesylate was significantly influenced by
the addition of sodium lauryl sulfate. In conditions where SMPT was expected, the addition of SLS at low concentrations (0.1–0.2 mM)
reduced the dissolution of haloperidol mesylate. In solutions containing concentrations of SLS above the critical micelle
concentration (CMC) (10–15 mM), the dissolution of haloperidol mesylate increased compared to below the CMC. The solids recovered
from solubility experiments of haloperidol mesylate indicated that haloperidol free base precipitated at all concentrations
of SLS. Above 5 mM of SLS, Raman microscopy suggested a new form, perhaps the estolate salt. The addition of surfactant in
solids that undergo solution-mediated phase transformation can add complexity to the dissolution profiles and conversion. 相似文献
4.
Porcel EM Foose LL Svitova TF Blanch HW Prausnitz JM Radke CJ 《Biotechnology and bioengineering》2009,102(5):1330-1341
Nonionic and ionic surfactants diminish the initial rate of proteolysis of aqueous bovine serum albumin (BSA) by subtilisin Carlsberg. Surfactants studied include: nonionic tetraethylene glycol monododecyl ether (C12E4); anionic sodium dodecyl sulfate (SDS), anionic sodium dodecylbenzenesulfonate (SDBS), and cationic dodecyltrimethylamonium bromide (DTAB). Kinetic data are obtained using fluorescence emission. Special attention is given to enzyme kinetic specificity determined by fitting initial-rate data to the Michaelis-Menten model. All surfactants reduce the rate of proteolysis, most strongly at concentrations near and above the critical micelle concentration (CMC). Circular dichroism (CD), tryptophan/tyrosine fluorescence spectra, and tryptophan fluorescence thermograms indicate that BSA partially unfolds at ionic surfactant concentrations near and above the CMC. Changes in BSA conformation are less apparent at ionic surfactant concentrations below the CMC and for the nonionic surfactant C12E4. Subtilisin Carlsberg activity against the polypeptide, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, decreased due to enzyme-surfactant interaction. At the concentrations and time frames studied, there was no enzyme autolysis. Importantly, aqueous proteolysis rates are significantly reduced at high surfactant concentrations where protein-micellar-surfactant aggregates occur. To explain the negative effect of surfactant on subtilisin Carlsberg proteolytic activity against BSA, we propose that micelle/protein complexes hinder enzyme access. 相似文献
5.
Enhancing and sustaining AMG 009 dissolution from a matrix tablet via microenvironmental pH modulation and supersaturation,
where poorly soluble acidic AMG 009 molecule was intimately mixed and compressed together with a basic pH modifier (e.g., sodium carbonate) and nucleation inhibitor hydroxypropyl methylcellulose K100 LV (HPMC K100 LV), was demonstrated previously. However, not all acidic or basic drugs are compatible with basic or acidic pH
modifiers either chemically or physically. The objective of this study is to investigate whether similar dissolution enhancement
of AMG 009 can be achieved from a bilayer dosage form, where AMG 009 and sodium carbonate are placed in a separate layer with
or without the addition of HPMC K100 LV in each layer. Study results indicate that HPMC K100 LV-containing bilayer dosage
forms gained similar dissolution enhancement as matrix dosage forms did. Bilayer dosage forms without HPMC K100 LV benefitted
the least from dissolution enhancement. 相似文献
6.
The interactions of hemoglobin (Hb) with sodium dodecyl sulfate (SDS) and dodecyl trimethylammonium bromide (DTAB) are investigated by several methods. We observed the formation of hemichrome below the critical micelle concentration (cmc) of surfactant and the release of heme from Hb above the cmc. When pH value of Hb/surfactant system is lower than isoelectric point (pI) of Hb, the interaction of SDS with Hb is both electrostatic and hydrophobic, while the interaction of DTAB with Hb is hydrophobic mainly. On the contrary, when pH > pI, the interaction of SDS with Hb is hydrophobic mainly, while the interaction of DTAB with Hb is both electrostatic and hydrophobic. In the case where both the electrostatic interaction and hydrophobic interaction exist, the electrostatic interaction plays a more important role. Thus, SDS tends to interact with Hb more obviously than DTAB does when pH < pI and the interaction between DTAB and Hb is stronger when pH > pI. 相似文献
7.
Depending on the dose size and solubility characteristics of low solubility drugs, a meaningful and discriminatory power of
dissolution rate testing can be demonstrated. Saturation solubility of fenofibrate and glipizide in different media were determined.
Solubility of fenofibrate increased directly with SLS concentration. For a 54-mg fenofibrate tablet, SLS at 0.025 M level
is required for a discriminative dissolution test, while for 160-mg tablet, dissolution condition and levels of SLS should
be optimized; higher concentrations may be effective (ie, 0.052 M, ∼1.5%). A pH 6.8 phosphate buffer medium is appropriate
for glipizide 10-mg tablet dissolution study, when formulation ingredients include excipients with surface activity (eg, HPMC). 相似文献
8.
Enzymatic degradation of immobilized ovalbumin multilayer films by subtilisin Carlsberg was investigated using in situ ellipsometry. Changes in the substrate cleavage rate in the presence of an anionic surfactant, sodium dodecylbenzene sulfonate (SDBS), were assessed. Exposure of the protein film to SDBS prior to introduction of the enzyme increased the measured proteolysis rate threefold. Surfactant increased the measured film thickness, absorbing into the protein film and causing swelling. Surfactant-induced film swelling was reversible upon aqueous rinsing. Nevertheless, exposure of enzyme to the surfactant-rinsed film increased the proteolysis rate, most likely due to irreversible conformational changes induced in the substrate film by the surfactant. Simultaneous addition of SDBS with enzyme after the initial surfactant exposure did not produce additional protein-removal benefit. 相似文献
9.
Abhay Gupta Robert L. Hunt Rakhi B. Shah Vilayat A. Sayeed Mansoor A. Khan 《AAPS PharmSciTech》2009,10(2):495-499
The purpose of the work was to investigate correlation between disintegration and dissolution for immediate release tablets
containing a high solubility drug and to identify formulations where disintegration test, instead of the dissolution test,
may be used as the acceptance criteria based on International Conference on Harmonization Q6A guidelines. A statistical design
of experiments was used to study the effect of filler, binder, disintegrating agent, and tablet hardness on the disintegration
and dissolution of verapamil hydrochloride tablets. All formulation variables, i.e., filler, binder, and disintegrating agent,
were found to influence tablet dissolution and disintegration, with the filler and disintegrating agent exerting the most
significant influence. Slower dissolution was observed with increasing disintegration time when either the filler or the disintegrating
agent was kept constant. However, no direct corelationship was observed between the disintegration and dissolution across
all formulations due to the interactions between different formulation components. Although all tablets containing sodium
carboxymethyl cellulose as the disintegrating agent, disintegrated in less than 3 min, half of them failed to meet the US
Pharmacopeia 30 dissolution criteria for the verapamil hydrochloride tablets highlighting the dependence of dissolution process
on the formulation components other than the disintegrating agent. The results identified only one formulation as suitable
for using the disintegration test, instead of the dissolution test, as drug product acceptance criteria and highlight the
need for systematic studies before using the disintegration test, instead of the dissolution test as the drug acceptance criteria.
The opinions expressed in this work are only of authors and do not necessarily reflect the policy and statements of the FDA. 相似文献
10.
José Rivera‐Utrilla María I. Bautista‐Toledo Manuel Sánchez‐Polo José D. Méndez‐Díaz 《Engineering in Life Science》2012,12(1):113-116
Successful surfactant removal from wastewater is often limited by the high concentration of the surfactant. The use of advanced oxidation processes can be the key to aid biological treatment of water containing high amounts of surfactants. The present study analyzes the biodegradation of the anionic surfactant sodium dodecylbenzenesulfonate (SDBS) and the effects of its combination with ozonation. SDBS pre‐ozonation favors the metabolism by microorganisms. Experimental results indicate that the application of a concentration of up to 60 μM of ozone for 60 min, prior to contact with microorganisms, increases the percentage of SDBS removed by biodegradation alone. These results demonstrate that the removal of SDBS and of the total organic carbon is increased by the consecutive use of ozonation and biodegradation. 相似文献
11.
百菌清(chlorothalonil)在水中的光化学降解 总被引:10,自引:0,他引:10
研究了光源种类、溶液pH、水温和表面活性剂对百菌清光解的影响.结果表明,百菌清水溶液在高压汞灯、紫外灯和太阳光照射下的光解半衰期分别为22.4、82.5和123.8 min;在太阳光和高压汞灯照射下,百菌清在碱性溶液中比中性和酸性溶液中光解快;随着水温的升高,百菌清光解速率加快,水温平均每升高10 ℃,光解速率大约增大1倍.表面活性剂十二烷基磺酸钠、Tween 60和Span 20对百菌清的光解表现出显著的光敏化效应,十六烷基三甲基溴化铵对百菌清光解有强烈的光猝灭效应. 相似文献
12.
The impact of surfactants on naphthalene and phenanthrene biodegradation and vice versa after surfactant flushing were evaluated using two anionic surfactants: sodium dodecyl sulfate (SDS) and sodium dodecyl benzene sulfonate (SDBS); and two nonionic surfactants: POE (20) sorbitan monooleate (T-maz-80) and octylphenol poly(ethyleneoxy) ethanol (CA-620). Naphthalene and phenanthrene biodegradation varied differently in the presence of different surfactants. Naphthalene biodegradation was not impacted by the presence of SDS. In the presence of T-maz-80 and CA-620, naphthalene biodegradation occurred at a lower rate (0.14 d-1 for T-maz-80 and 0.19 d-1 for CA-620) as compared to un-amended control (0.29 d-1). Naphthalene biodegradation was inhibited by the presence of SDBS. In the presence of SDS, phenanthrene biodegradation occurred at a lower rate (0.10 d-1 as compared to un-amended control of 0.17 d-1) and the presence of SDBS, CA-620 and T-maz-80 inhibited phenanthrene biodegradation. The surfactants also responded differently to the presence of naphthalene and phenanthrene. In the presence of naphthalene, SDS biodegradation was inhibited; SDBS and T-maz-80 depleted at a lower rate (0.41d-1 and 0.12 d-1 as compared to 0.48 d-1 and 0.22 d-1). In the absence of naphthalene, CA-620 was not degradable, while in the presence of naphthalene, CA-620 began to degrade at a comparatively low rate (0.12 d-1). In the presence of phenanthrene, SDS biodegradation occurred at a lower rate (1.2 d-1 as compared to 1.68 d-1) and a similar trend was observed for T-maz-80. The depletion of SDBS and CA-620 did not change significantly. The choice of SDS for naphthalene-contaminated sites would not adversely affect the natural attenuation of naphthalene, in addition, naphthalene was preferentially utilized to SDS by naphthalene-acclimated microorganisms. Therefore, SDS was the best choice. T-maz-80 was also found to be usable in naphthalene-contaminated sites. For phenanthrene contaminated sites, SDS was the only choice. 相似文献
13.
A novel optical activity of lutein was studied in dodecyltrimethylammonium bromide (DTAB) solution by the measurement of circular dichroism and absorbance. The surfactant was found to bring about the circular dichroism activity of the lutein below the critical micelle concentration (CMC) in a different way from that by sodium dodecyl sulfate (SDS). This phenomenon was interpreted by the card-pack model of the lutein aggregate in which lutein molecule was slightly shifted each other. The above optical activity abruptly became strong just before the CMC of DTAB. This seems to correspond to the transition from the polymeric aggregate of the lutein to the oligomeric one. Such an optical activity disappeared beyond the CMC on the incorporation of the lutein molecules into the surfactant micelles. The molar binding ratios of DTAB to the lutein were determined to be 130 to 210 on the basis of the lutein concentration dependence of the DTAB concentration showing the arbitrary ellipticity. These ratios were clearly larger than those for SDS. On the other hand, filtration measurement showed that the size of the lutein-DTAB complex was larger than 2 μm in diameter. These phenomena were discussed assuming the possible model of the aggregate as a comparative study of the anionic and cationic surfactants causing the novel optical activity of this aggregate. 相似文献
14.
Elena Hutchens Eugenia Valsami-Jones Najatte Harouiya Claire Chaïrat Eric H. Oelkers Sharron McEldoney 《Geomicrobiology journal》2013,30(3-4):177-182
Field observations suggest that some mineral dissolution rates can be enhanced by microbial activity indirectly, without direct contact with the mineral surface. A series of apatite dissolution experiments was performed to better understand this rate enhancement process. Far-from equilibrium abiotic apatite dissolution rates, measured in mixed-flow reactors at 25°C were enhanced by increasing concentration of aqueous organic acids and decreasing aqueous phosphate activity, demonstrating the existence of indirect pathways for microbial rate enhancement. Further apatite dissolution experiments were performed in closed-system reactors in the presence of Bacillus megaterium , a common heterotrophic aerobe. Experiments were designed to allow the bacteria to be either in direct contact or indirect contact with the apatite; in the latter case, the microbes were physically separated from the apatite using dialysis bags. Apatite dissolution in indirect contact with Bacillus megaterium was 50 to 900% faster than abiotic controls. Bacterial rate enhancement was, however, 3 to over 10 times lower when Bacillus megaterium was in direct contract versus indirect contact with the apatite surfaces. These results show that (1) bacteria can accelerate rates without being in physical contact with the dissolving mineral, and (2) microbially mediated dissolution may be less effective when bacteria are in direct contact with mineral surfaces. Supression of mineral dissolution is interpreted to stem from the preferential colonization of reactive sites on the mineral surface. 相似文献
15.
Francesca Sansone Alessandra Rossi Pasquale Del Gaudio Francesco De Simone Rita Patrizia Aquino Maria Rosaria Lauro 《AAPS PharmSciTech》2009,10(2):391-401
Gastroresistant microparticles for oral administration of hesperidin (Hd) were produced by spray-drying using cellulose acetate
phthalate (CAP) as enteric polymer in different polymer/Hd weight ratio (1:1, 3:1, and 5:1), and a series of enhancers of
the dissolution rate, such as sodium carboxymethylcellulose crosslinked (CMC), sodium dodecylbenzene sulfonate (SDBS), or
Tween85. The raw materials and the microparticles were investigated by differential-scanning calorimetry, X-ray diffraction,
infrared spectroscopy and imaged using scanning electron and fluorescence microscopy. In vitro dissolution tests were conducted using a pH-change method to investigate the influence of formulative parameters on the dissolution/release
properties of the drug. CAP/Hd microparticles showed a good gastro-resistance but incomplete drug dissolution in the simulated
intestinal fluid (SIF). The presence of the enhancers in the formulation produced well-formed microparticles with different
size and morphology, containing the drug well coated by the polymer. All the enhancers were able to increase the dissolution
rate of Hd in the simulated intestinal environment without altering CAP ability to protect Hd in the acidic fluid. The spray-drying
technique and process conditions selected were effective in microencapsulating and stabilizing the flavonoid giving satisfactory
encapsulation efficiency, product yield, and microparticles morphology, and a complete drug release in the intestine. 相似文献
16.
Mohammed R. Housaindokht Ali A. Moosavi-Movahedi Jaliel Moghadasi Malcolm N. Jones 《International journal of biological macromolecules》1993,15(6):337-341
The enthalpies of interaction of glucose oxidase at 25°C with a homologous series of n-alkyltrimethylammonium bromides (TABs) at pH 10 and a homologous series of n-alkylsulfates at pH 3.2 have been measured by microcalorimetry. For the n-dodecyl member of each series, DTAB and sodium n-dodecylsulfate (SDS), the binding of the surfactants to glucose oxidase as measured by equilibrium dialysis has been used in combination with the enthalpy data to obtain the Gibbs energy (
), enthalpy (
) and entropy (
) of binding per surfactant molecule as a function of the number of surfactant molecules bound (
). The thermodynamic parameters for the glucose oxidase interaction with DTAB at pH 10 and SDS at pH 3.2 are very similar and show that the interactions are entropically driven. The observed enthalpies of interaction of glucose oxidase with the homologous n-alkylsulfates have been analysed in terms of the interactions between the anionic surfactant head group and cationic sites on the protein, hydrophobic binding and the thermal contributions arising from protein unfolding. At surfactant concentrations of 0.5 c.m.c., the enthalpy of unfolding of glucose oxidase is estimated to be 3610 ± 560 kJ mol−1. 相似文献
17.
The role of poloxamer 188, water and binder addition rate, on retarding dissolution in immediate-release tablets of a model
drug from BCS class II was investigated by means of multivariate data analysis (MVDA) combined with design of experiments
(DOE). While the DOE analysis yielded important clues into the cause-and-effect relationship between the responses and design
factors, multivariate data analysis of the 40+ variables provided additional information on slowdown in tablet dissolution.
A steep dependence of both tablet dissolution and disintegration on the poloxamer and less so on other design variables was
observed. Poloxamer was found to increase dissolution rates in granules as expected of surfactants in general but retard dissolution
in tablets. The unexpected effect of poloxamer in tablets was accompanied by an increase in tablet-disintegration-time-mediated
slowdown of tablet dissolution and by a surrogate binding effect of poloxamer at higher concentrations. It was additionally
realized through MVDA that poloxamer in tablets either acts as a binder by itself or promotes binder action of the binder
povidone resulting in increased intragranular cohesion. Additionally, poloxamer was found to mediate tablet dissolution on
stability as well. In contrast to tablet dissolution at release (time zero), poloxamer appeared to increase tablet dissolution
in a concentration-dependent manner on accelerated open-dish stability. Substituting polysorbate 80 as an alternate surfactant
in place of poloxamer in the formulation was found to stabilize tablet dissolution. 相似文献
18.
Laís Bastos da Fonseca Márcio Labastie Valéria Pereira de Sousa Nadia Maria Volpato 《AAPS PharmSciTech》2009,10(4):1145-1152
The dissolution test for oral dosage forms has recently widened to a variety of special dosage forms such as suspensions.
For class II drugs, such as nimesulide (NMS), this study is very important because formulation problems may compromise drug
bioavailability. In the present work, tests with four brands of commercially available NMS (RA, TS, TB, and TC) have been
performed in order to study their dissolution at different conditions. The suspensions have been characterized relatively
to particle size, pH, and density besides NMS assay and the amount of drug in solution in the suspension vehicles. The dissolution
study was conducted using the following media: simulated intestinal fluid, pH 6.8, containing polysorbate 80 (P80) or sodium
lauryl sulfate (SLS); phosphate buffer, pH 7.4, with P80 and aqueous solution of SLS. Concerning the quantitative analysis,
the UV–VIS spectrophotometry could have been used in substitution to high-performance liquid chromatography since the methodology
had been adequately validated. The influence of the drug particle size distribution was significant on the dissolution profiles
of NMS formulations, confirming to be a factor that should be strictly controlled in the development of oral suspensions. 相似文献
19.
20.
Useful materials can be made from cycloamylose (CA) and the functional properties of CA could be improved by complexation with surfactants. Isothermal titration calorimetry (ITC) was used to investigate interactions between CA and surfactants in buffered solutions. Three surfactants with C12 non-polar tail groups and charged [anionic: sodium dodecyl sulfate (SDS); cationic: dodecyl trimethylammonium bromide (DTAB)] or non-charged headgroups [non-ionic: polyoxyethylene 23 lauryl ether (Brij35)] were used in this study. The effects of temperature, pH, and salt concentration were also studied. All three surfactants bound to CA; however, Brij35 binding to CA was negligible. Enthalpy changes associated with binding of surfactants to CA were exothermic except for interactions measured at 50 °C. There was no effect of pH on surfactant demicellization or CA binding. Salt concentration affected surfactant demicellization, but the amount of SDS bound to CA at saturation was unaffected by salt. When the titration curves obtained for CA with SDS and DTAB were fitted, it could be analyzed using a model based on a single set of identical sites. 相似文献