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同色兜兰的组织培养与快速繁殖 总被引:4,自引:0,他引:4
1植物名称同色兜兰[Paphiopedilum concolor(Bateman)Pfitz.]。
2材料类别种子。
3培养条件种子萌发培养基:(1)MS+100mL·L^-1香蕉汁;(2)1/2MS+100mL·L^-1香蕉汁。原球茎继代增殖培养基:(3)1/2MS+NAA0.2mg·L^-1(单位下同)+100mL·L^-1香蕉汁;(4)1/2MS+NAA0.4+100mL·L^-1香蕉汁;(5)1/2MS+6-BA0.5+NAA0.2+100mL·L^-1。 相似文献
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1植物名称蚊子花(Lopezia cordata Hornem.)。
2材料类别茎段和顶芽。
3培养条件基本培养基为MS。(1)芽诱导培养基:1/2MS(MS大量元素用量减半);(2)增殖培养基:MS+6-BA0.2mg·L^-1(单位下同)+NAA0.2;(3)生根培养基:I/2MS+NAA0.5。以上培养基中均加入6g·L^-1琼脂和30g·L^-1蔗糖,pH5.9—6.0。培养室中温度为(24+1)℃,光照时间为16h·d^-1,光照强度约为60μmol·m^-2·s^-1。 相似文献
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1植物名称欧洲小叶椴(Tilia cordata Mill.)。
2材料类别成熟胚。
3培养条件(1)/3动培养基:MS+6-BA2.0mg·L^-1(单位下同)+NAA0.1;(2)增殖培养基:MS+6.BA1.0+NAA0.1;(3)壮苗生根培养基:1/2MS+IBA1.0+NAA0.3。以上培养基均加入0.55%琼脂和20g·L^-1。蔗精,pH5.8-6.0。培养温度为(25±2)℃;光照培养时间12h·d^-1,光照强度40.50μmol·m^-2·s^-1。 相似文献
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以石龙尾(Limnophila sessiliflora BI.)沉水枝带节茎段为外植体进行离体培养,研究外植体灭菌方法以及培养基中不同生长调节剂的浓度对其增殖、生根的影响。结果表明:以0.1%的HgCl2为灭菌剂,采用4min+4min、间歇4h的间歇灭菌法,可以获得成活的无菌外植体15%;在1/2MS+6.BA2.0mg·L^-1+NAA 0.1~0.2mg·L^-1的增殖培养基上培养35d,试管苗的增殖系数可达30.8以上;在1/2MS+6-BA 0.3mg·L^-1+NAA 0.5mg·L^-1的生根培养基上培养28d后,可获得具3~5个侧枝的生根苗,平均每株生根数4.8条;炼苗后移植成活率100%。 相似文献
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Although the importance of the endosperm as a food store inmany angiosperm seeds is well known, its significance duringearly embryogenesis has been neglected. In many interspecifichybrids, and in some other situations, embryos do not developfully and abort. It has often been stated that this is causedby the endosperm failing to conduct sufficient nutrients tothe embryo, but seldom has it been suggested that the endospermactively controls most of the early stages of morphogenesisof the embryo. Information gleaned from a broad survey of theliterature, combined with additional evidence presented here,obtained fromSolanum incanumand interspecific hybrids, indicatethat the endosperm is dynamic and very active in regulatingearly embryo development. This requires highly integrated geneticcontrol of rapidly changing metabolism in the endosperm. Ininterspecific hybrids, lack of coordination may cause unbalancedproduction of growth regulating substances by the endospermand hence abortion of the embryo, or even unregulated productionof nucleases and proteases resulting firstly in autolysis ofthe endosperm and then digestion of the embryo. The endospermmay thus serve to detect inappropriate hybridization of speciesor ploidy levels and so prevent waste of resources by producingseeds that would result in sterile hybrids or unthrifty subsequentgenerations. This discriminatory function of the endosperm hasdiminished during evolution and domestication of the crop plantSolanummelongenaL.Copyright 1998 Annals of Botany Company Solanum, embryo morphogenesis, endosperm, hybrid, seed development. 相似文献
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Höpfl G Ogunshola O Gassmann M 《American journal of physiology. Regulatory, integrative and comparative physiology》2004,286(4):R608-R623
For most organisms oxygen is essential fo life. When oxygen levels drop below those required to maintain the minimum physiological oxygen requirement of an organism or tissue it is termed hypoxia. To counter act possible deleterious effects of such a state, an immediate molecular response is initiated causing adaptation responses aimed at cell survival. This response is mediated by the hypoxia-inducible factor-1 (HIF-1), which is a heterodimer consisting of an alpha- and a beta-subunit. HIF-1 alpha protein is stabilized under hypoxic conditions and therefore confers selectivity to this response. Hypoxia is characteristic of tumors, mainly because of impaired blood supply resulting from abnormal growth. Over the past few years enormous progress has been made in the attempt to understand how the activation of the physiological response to hypoxia influences neoplastic growth. In this review some aspects of HIF-1 pathway activation in tumors and the consequences for pathophysiology and treatment of neoplasia are discussed. 相似文献
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Michele Rivkin-Flsh 《Medical anthropology quarterly》2000,14(1):119-120
Pragmatic Women and Body Politics. Margaret Lock and Patricia A. Kaufert. eds. Cambridge, UK: Cambridge University Press, 1998. xii +364 pp.
Maternities and Modernities: Colonial and Postcolonial Experiences in Asia and the Pacific. Kalpana Ram and Margaret Jolly. eds. Cambridge, UK: Cambridge University Press, 1998. xiv. 305 pp. 相似文献
Maternities and Modernities: Colonial and Postcolonial Experiences in Asia and the Pacific. Kalpana Ram and Margaret Jolly. eds. Cambridge, UK: Cambridge University Press, 1998. xiv. 305 pp. 相似文献
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Studies of human constitution and mental features are traditionally among the main concepts of anthropological research. In
this research, we attempted to discover connections between morphological and constitutional features and self-esteem in children
and adolescents. Such a connection was discovered in some studied age groups. The obtained results could serve as a basis
for further studies in this field. 相似文献