共查询到20条相似文献,搜索用时 31 毫秒
1.
Alan W. White 《Journal of phycology》1974,10(3):292-300
The facultative, heterotrophs Cyclotella cryptica (sclone WT-1-8) and Coscinodiscus sp. were selectively isolated from coastal waters by dark incubation of organically enriched solid medium. C. cryptica grows in the dark with glucose and galactose, and Coscinodiscus sp. with glucose. Clone WT-1-8 of C. cryptica grows about twice as fast with glucose as a previously studied clone (0-3A). In the dark with, 5 × 10?6 M glucose C. cryptica divides every 3 days, while with 5 × 10?5 M glucose Coscinodiscus sp. divides every 10 days. Heterotrophic growth of either diatom for 1 year does not cause a major reduction in carbon, nitrogen, chlorophyll a, and chlorophyll c contents, or in photosynthetic ability, compared to light-grown cells. It is possible that facultative heterotrophy is of ecological benefit to these diatoms, probably for slow growth and survival during extended periods of dim light or darkness. 相似文献
2.
Johan A. Hellebust 《Journal of phycology》1971,7(1):1-4
Growth rate as well as rate of glucose uptake of C. cryptica depends on glucose concentration in the medium according to saturation kinetics. The K g for growth is 1.9 × 10?5 M, and the K t, for glucose transport is 5.8 × 10?5 M. The maximum growth rate in the dark on glucose is considerably slower than the light-saturated growth rate at the same temperature, and does not appear to be determined by the capacity of the cell for glucose uptake. The glucose transport process is highly specific, and depends on energy metabolism. The Q 10 for the process is 2.2 (15–2.5 C). Glucose taken up by the cells is almost, quantitatively phosphorylated within 10 min, either through the transport process itself or by a high affinity kinase system in the cells. 相似文献
3.
The glucose uptake of a species of Chlamydomonas was studied at various concentrations of d -glucose plus glucose-1-14C (0.003–10.0 mg/liter) and at various light levels (0–220 ft-c). The alga grows at 4 C either in the light or in the dark with added glucose, cellobiose, maltose, or fructose. Uptake of glucose could be described by the Michaelis-Menten equation, and both the maximum velocity of uptake and the half-saturation constant increased when the cells were exposed to glucose in the dark. However, the high value of the half-saturation constant (5 mg glucose/liter) compared with the low levels of glucose in nature (5–10 μg/liter) makes it unlikely that a transport system is effective under natural conditions. Even if a total of 10.0 mg/liter of glucose plus other organic compounds were available as substrate, the rate of photosynthesis would still be more than 10 times higher (at 220 ft-c) than the rate of organic substrate uptake. Light had no effect on the total uptake of glucose but did reduce the percentage of 14CO2 evolved from 61% of the total 14CO taken up in the dark to 0% at 220 ft-c. This decrease could be due to either preferential use of the 14CO2 in photosynthesis or of the photosynthate in respiration. 相似文献
4.
Johan A. Hellebust 《Journal of phycology》1971,7(4):345-349
Glucose transport capacity of C. cryptica increases in an exponential manner over 24 hr after transfer of the cells from light to complete darkness with little simultaneous increase in cell number. The transport system is rapidly inactivated when cells are transferred back to continuous light. Most of the inactivation takes place while there is still little changes in cell number. When grown on a continuous light regime, the capacity for glucose transport per cell depends on the light intensity. At intensities sufficient to saturate photosynthesis the glucose transport system is only about 5% that of dark-grown cells, while cells grown at intensities close to the light compensation point have about 30% of the capacity of dark-grown cells. The action spectrum for inactivation of glucose transport is identical to that for photosynthesis. Cells, whether grown under continuous light, in the dark in the presence of glucose, or kept in the dark without glucose, contain high levels of glucokinase and phosphofructokinase. The glucose transport system is highly specific for glucose; only galactose inhibits the uptake of glucose by about 50% when present at 10 times the concentration of glucose. The glucokinase is even more specific for glucose and is not inhibited by galactose. The phosphofructokinase is inhibited by high concentrations of ATP in cells grown under all conditions. cycloheximide inhibits the induction of glucose transport in the dark, but not the inactivation of the system in the light. 相似文献
5.
Melosira nummuloides, clone Mel-3, shows a very high specificity with regard to its ability to take up organic substrates. Amino acids supplied in the medium at 1 X 10-4 M are taken up at initial rates of the same order of magnitude as that of photoassirnilation of COj. However, sugars, sugar alcohols, or organic acids supplied at the same concentration are not taken up. The mechanism for uptake of amino acids appears to require energy, since tlie uptake of the amino acid analog α-aminoisobutyric acid is strongly inhibited by 2 f-dinitrophenul. The uptake mechanism does not appear to be inducible. The ability of M. numinuloides to utilize amino acids as a nitrogen source is quite restricted. Arginine, ghttamine, asparagine, proline, and glutamic acid were good nitrogen sources. Seventeen other amino acids, including α-aminoisobutyric acid, were unsatisfactory for growth, although they were rapidly taken up from the medium. 相似文献
6.
1. Planktothrix rubescens is the dominant photoautotrophic organism in Lake Zürich, a prealpine, deep, mesotrophic freshwater lake with an oxic hypolimnion. Over long periods of the year, P. rubescens accumulates at the metalimnion and growth occurs in situ at irradiance near the photosynthesis compensation point. Experiments were conducted to evaluate the contribution of photoheterotrophy, heterotrophy and light‐dependent uptake of nitrogenous organic compounds to the carbon and nitrogen budget of this cyanobacterium under conditions of restricted availability of light quanta. 2. We used both purified natural populations of P. rubescens from the depth of 9 m and an axenic culture grown under low irradiance at 11 μmol m?2 s?1 on a light : dark cycle (10 : 14 h) to determine the uptake rates of various amino acids, urea, glucose, fructose, acetate and inorganic carbon. The components were added to artificial lake water in low amounts that simulated the naturally occurring potential concentrations. 3. The uptake rates of acetate and amino acids (glycine, serine, glutamate and aspartate) were strongly enhanced at low irradiance as compared with the dark. However, no difference was observed in the uptake of arginine, which was taken up at high rates under both treatments. The uptake rates of glucose, fructose and urea were very low under all conditions. Similar results were obtained for both axenic P. rubescens and for purified natural populations of P. rubescens that were separated from bacterioplankton and other phytoplankton. 4. Metalimnetic P. rubescens that was stratified at low irradiance for weeks exhibited much higher uptake rates than filaments that were entrained in the deepening surface mixed layer and experienced higher irradiance. The added organic compounds contributed up to 62% to the total carbon uptake of metalimnetic P. rubescens. On the basis of a molar C : N ratio of 4.9, the nitrogen uptake as organic compounds satisfied up to 84% of the nitrogen demand. 5. The experiments indicate that photoheterotrophy and light‐dependent uptake of nitrogenous organic compounds may contribute significantly to the carbon and nitrogen budget of filaments at low irradiance typical for growth of P. rubescens in the metalimnion and at the bottom of the surface mixed layer. 相似文献
7.
Glucose, galactose and galacturonic acid were taken up at different rates by the fungus Rhynchosporium secalis and were intracellularly converted to other forms of carbohydrate at different rates. These differences explain why, when there is only a single source of nutrient carbon in the growth medium, development of the fungus is greatest when glucose is present and least when galactose is present. Glucose and galactose were taken up by the same mechanism for their uptake showed a reciprocal competitive inhibition. Uptake mechanisms had a high affinity for glucose (apparent Km 2.76 mM) and galacturonic acid (apparent Km 3.10 mM) and a low affinity for galactose (apparent Km 29.67 mM). After uptake, galactose accumulated in the mycelium, whereas glucose and galacturonic acid were rapidly converted to other soluble carbohydrates, principally trehalose and mannitol. The insoluble carbohydrates within the mycelium were little affected by the type of carbohydrate that was supplied to the fungus. 相似文献
8.
Summary A mutant of Aspergillus nidulans lacking galactose phosphate-UDP glucose transferase could not grow on galactose but incorporated this sugar into cell constituents when supplied with another carbon source. 75% of the radioactivity taken up was found in the galactose and glucose monomers of the hyphal wall. Most of the remaining label was in a cytoplasmic polysaccharide and in free galactose and galactose phosphate. The composition of the cytoplasmic polysaccharide resembled that of the wall polymers. These findings are taken to indicate that enzymes not connected with the Leloir pathway can activate and epimerise galactose and that polymeric wall precursors may be present in the cytoplasm. The specific labelling obtained with galactose was combined with radioautography to show that glucose and galactose containing polymers are incorporated into the hyphal wall at the growing tip. 相似文献
9.
Prototheca zopfii (12 strains) is able to use glucose, fructose, propanol, glycerol, and acetate as sources of carbon for growth. One of the strains is biochemically (utilization also of galactose and mannose), and two strains are morphologically slightly different.Two strains can be identified as P. wikerhamii. They exhibit good growth with glucose, fructose, galactose, trehalose, propanol, glycerol, acetate, and glutamate as sources of carbon.
P. spec. 263-2 grows only with glucose and acatate. P. zopfii and P. wickerhamii are able to use urea, glycine, and glutamate as sources of nitrogen. P. spec. 263-2, on the other hand, cannot utilize these organic nitrogen compounds for growth.Four strains of Chlorella protothecoides are able to use glucose, fructose, galactose, and acetate as sources of carbon for growth in the dark. Three of them utilize also mannose, trehalose, and glutamate. Two strains can grow with glycerol, and one is able to use lactose. — Urea and glycine can serve as sources of nitrogen for the four strains of C. protothecoides. Glutamate supports growth of three strains, and one strain is able to use nicotinamide. 相似文献
10.
Johan A. Hellebust 《Journal of phycology》1978,14(1):79-83
The uptake of glucose and amino acids by the euryhaline diatom Cyclotella cryptica Reimann, Lewin & Guillard does not appear to be related to proton gradients. Instead, the transport systems for these organic solutes show a strong requirement for the presence of NaCl. The relationship between uptake and NaCl concentration is hyperbolic, with optimal uptake rates being approached at 100 mM NaCl. High concentrations of KCl cause strong reductions in uptake rates. The (Na+, K+)-stimulated ATPase inhibitor ouabain has no effect on glucose uptake, whereas the diphenolic glucoside phlorizin and its aglucone phloretin are strongly inhibitory. The proton translocating uncoupler CCCP (carbonylcyanide m-chlorophenyl hydrazone) and the ATPase inhibitor DCCD (dicyclohexylcarbodiimide) both almost completely abolish glucose transport, and low concentrations of the ionophares monensin and valenomycin strongly inhibit glucose uptake by the diatom. The requirement of high external NaCl concentrations for glucose transport, and the inhibitory effect an transport of the Na+-specific ionophore monensin are consistent with a coupling of Na+ and organic substrate transport, but could also be explained by a Na+ requirement for glucose binding to a transport carrier, and/or a possible interference with energy producing reactions associated with a monensin-induced collapse of the normal Na+ gradient. 相似文献
11.
Florida's red tide organism, Gymnodinium breve, utilized exogenous glucose in the light for the synthesis of cellular components. Glucose was not taken up in the dark. Kinetic parameters for glucose uptake include a KFD of 11 μM and a Vmax of 1 × 10?10 mol of glucose taken up/mg cellular protein/hr. Glucose uptake was competitively inhibited by phloridzin (Ki = 40 μM), mannose (Ki = 12O μM), and 2-deoxy-d-glucose (Ki = 190 μM) and non-competitively inhibited by galactose (Ki = 125 μM). Kinetics and inhibition of glucose uptake are consistent with a facilitated diffusion transport system. 相似文献
12.
The green colonial alga Botryococcus braunii is characterized by the ability to produce and accumulate large amounts of hydrocarbons. We isolated and established an axenic
clonal strain of B. braunii B70 and investigated the effects of organic carbon sources, including glucose, mannose, fructose, galactose, or acetate,
on growth under light and dark conditions. This algal strain had the capacity to grow photo-, mixo-, or heterotrophically.
Growth was promoted substantially following exposure of the algae to glucose or mannose under light exposure. Cells could
grow under continuous darkness with glucose or mannose. In the presence of glucose under light or dark conditions, cell and
colony size, and the intracellular granules containing oil, were markedly larger than those cultured without glucose. 相似文献
13.
Chlorella sorokiniana strain 211-40c, a symbiotic Chlorella isolated from a freshwater sponge, excreted between 3% and 5% of assimilated 14CO2 as glucose in the light, with a pH optimum around 5. This percentage increased when the illuminance was lowered (to 15% at 20 lx). Release of [14C]glucose continued in the dark and could be inhibited by the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Net efflux of glucose occurred even at a concentration ratio of extracellular/intracellular glucose of 4. This, together with the sensitivity to FCCP, is taken as evidence for active transport. Exogenous [14C]glucose was taken up by the cells under conditions of net glucose efflux, showing uptake and excretion to take place simultaneously.Abbreviations FCCP
carbonyl cyanide p-trifluoromethoxyphenylhydrazone
- p.c.
packed cells 相似文献
14.
Srdjan Novak Tony D'Amore Inge Russell Graham G. Stewart 《Journal of industrial microbiology & biotechnology》1990,6(2):149-155
Summary A number of 2-deoxy-d-glucose (2-DOG) resistant mutants exhibiting resistance to glucose repression were isolated from variousSaccharomyces yeast strains. Most of the mutants isolated were observed to have improved maltose uptake ability in the presence of glucose. Fermentation studies indicated that maltose was taken up at a faster rate and glucose taken up at a slower rate in the mutant strains compared to the parental strains, when these sugars were fermented together. When these sugars were fermented separately, only the 2-DOG resistant mutant obtained fromSaccharomyces cerevisiae strain 1190 exhibited alterations in glucose and maltose uptake compared to the parental strain. Kinetic analysis of sugar transport employing radiolabelled glucose and maltose indicated that both glucose and maltose were transported with higher rates in the mutant strain. These results suggested that the high affinity glucose transport system was regulated by glucose repression in the parental strain but was derepressed in the mutant. 相似文献
15.
Dynamics in the production of extracellular polymeric substances (EPS) were investigated for the benthic diatoms Cylindrotheca closterium (Ehrenberg) and Nitzschia sp. The effect of growth phase and light:dark conditions were examined using axenic cultures. Two EPS fractions were distinguished. Soluble EPS was recovered from the culture supernatant and represented polysaccharides that were only loosely associated with the cells. Bound EPS was extracted from the cells using warm (30° C) water and was more closely associated with the diatom aggregates. Concentrations of EPS exceeded internal concentrations of sugar throughout growth, indicating that EPS production is important in these organisms. Soluble and bound EPS revealed distinct differences in daily dynamics during the course of growth. Soluble EPS was produced continuously once cultures entered the stationary phase. During the stationary phase, chl a‐normalized EPS production rates equaled 6.4 and 3.4 d ? 1 for C. closterium and Nitzschia sp., respectively. In contrast, production of bound EPS occurred only in the light and was highest during the exponential phase. Up to 90% of the attached EPS that was produced in the light was degraded during the subsequent dark period. The monosaccharide distribution of EPS was constant during the course of the experiment. The soluble EPS consisted of high amounts of galactose and glucuronic acid, relative to rhamnose, glucose, xylose/mannose, and galacturonic acid. In contrast, glucose was the dominant monosaccharide present in the bound EPS. These differences suggest that the production of the two distinct EPS fractions is under different metabolic controls and probably serves different cellular functions. 相似文献
16.
Anders Torstensson Jodi N. Young Laura T. Carlson Anitra E. Ingalls Jody W. Deming 《Journal of phycology》2019,55(3):663-675
Wide salinity ranges experienced during the seasonal freeze and melt of sea ice likely constrain many biological processes. Microorganisms generally protect against fluctuating salinities through the uptake, production, and release of compatible solutes. Little is known, however, about the use or fate of glycine betaine (GBT hereafter), one of the most common compatible solutes, in sea‐ice diatoms confronted with shifts in salinity. We quantified intracellular concentrations and used [14C]‐labeled compounds to track the uptake and fate of the nitrogen‐containing osmolyte GBT and its precursor choline in three Antarctic sea‐ice diatoms Nitzschia lecointei, Navicula cf. perminuta, and Fragilariopsis cylindrus at ?1°C. Experiments show that these diatoms have effective transporters for GBT, but take up lesser amounts of choline. Neither compound was respired. Uptake of GBT protected cells against hyperosmotic shock and corresponded with reduced production of extracellular polysaccharides in N. lecointei cells, which released 85% of the retained GBT following hypoosmotic shock. The ability of sea‐ice diatoms to rapidly scavenge and release compatible solutes is likely an important strategy for survival during steep fluctuations in salinity. The release and recycling of compatible solutes may play an important role in algal–bacterial interactions and nitrogen cycling within the semi‐enclosed brines of sea ice. 相似文献
17.
Effects of hydrocarbons on the marine diatomCyclotella cryptica have been studied in laboratory experiments. Low hydrocarbon concentrations (100 g· l–1) stimulate growth whereas higher concentrations (l mg· l–1) inhibit growth. The aromatics are most toxic among the hydrocarbons, whereas the paraffins do not seem to have any serious effect on growth or photosynthesis of the algae. Both aromatic and paraffin fractions affected the chlorophyll a, protein, and sugar contents of the cells. Toxicity levels were affected by the presence of dissolved organic material in the medium. Studies on the ultrastructure ofC. cryptica have shown that paraffins affect the thickness of the cell wall. 相似文献
18.
Voitsekhovskaya O. V. Heber U. Wiese C. Lohaus G. Heldt H.-W. Gamalei Yu. V. 《Russian Journal of Plant Physiology》2002,49(1):44-53
Solutions of sucrose, glucose, raffinose, and stachyose were fed via the petiole to detached leaves of plant species known to transfer sugars during photosynthesis into the phloem using either the apoplastic or the symplastic pathway of phloem loading. Symplastic phloem loaders, which translocate raffinose-type oligosaccharides and sucrose in the phloem, and apoplastic plants, translocating exclusively sucrose, were selected for this study. As the sugars arrived with the transpiration stream in the leaf blade within little more than a minute, dark respiration increased. Almost simultaneously, fluorescence of a potential-indicating dye, which had been infiltrated into the leaves, indicated membrane depolarization. Another fluorescent dye used to record the apoplastic pH revealed apoplastic alkalinization that occurred with a slight lag phase after respiration and membrane depolarization responses. Occasionally, alkalinization was preceded by transient apoplastic acidification. Whereas membrane depolarization and apoplastic acidification are interpreted as initial responses of the proton motive force across the plasma membrane to the advent of sugars in the leaf apoplast, the following apoplastic alkalinization showed that sugars were taken up from the apoplast into the symplast in cotransport with protons. This was true not only for glucose and sucrose, but also for raffinose and stachyose. Similar observations were made for sugar uptake not only in leaves of plants known to export sugars by symplastic phloem loading but also of plants using the apoplastic pathway. Increased respiration during sugar uptake revealed tight coupling between respiratory ATP production and ATP consumption by proton-translocating ATPase of the plasma membrane, which exports protons into the apoplast, thereby compensating for the proton loss in the apoplast when protons are transported together with sugars into the symplast. The extent of stimulation of respiration by sugars indicated that sugar uptake was not limited to phloem tissue. Ratios of the extra CO2 released during sugar uptake to the amounts of sugars taken up were variable, but lowest values were lower than 0.2. When a ratio of 0.2 is taken as a basis to calculate rates of sugar uptake from observed maxima of sugar-dependent increases in respiration, rates of sugar uptake approached 350 nmol/(m2 leaf surface s). Sugar uptake rates were half-saturated at sugar concentrations in the feeding solutions of about 10–25 mM indicating a low in vivo affinity of sugar uptake systems for sugars. 相似文献
19.
Andreas Nicklisch 《International Review of Hydrobiology》1999,84(3):233-241
The nutrient saturated growth and the competition between Limnothrix redekei and some spring diatoms for phosphate were studied in semi-continuous cultures at 10°C with a 6/18 h light/dark cycle and at 15 °C with a 12/12 light/dark cycle. Under these conditions in unialgal cultures, the maximum specific growth rate of Limnothrix redekei was two to three time lower than that of the diatoms. However, Limnothrix redekei was the superior competitor for phosphate under both conditions in mixed cultures. The diatoms in mixed cultures from each species with Limnothrix redekei were competitively displaced but at a different rate. This allowed the diatoms to be ranked in terms of their competitive ability for phosphate at 10°C as follows: Synedra acus > Asterionella formosa > Stephanodiscus minutulus > Nitzschia acicularis > Cyclotella meneghiniana > Stephanodiscus neoastrea > Stephanodiscus hantzschii. The competitive ability of Limnothrix redekei at 15 °C was increased and the rank order of diatoms slightly changed. The hypothesis that cyanobacteria are promoted by phosphate because they are inferior competitors for phosphate is incorrect for Limnothrix redekei. 相似文献
20.