Generator locations of movement-related potentials with tongue protrusions and vocalizations: subdural recording in human

Movement-related potentials (MRPs) associated with tongue protrusions and vocalizations were recorded from chronically implanted subdural electrodes over the lower perirolandic area in 7 patients being evaluated for epilepsy surgery. In 3 patients, tongue protrusions elicited a clearly defined, well localized slow negative Bereitschaftspotential (BP) at the motor tongue area, and a positive BP at the sensory tongue area. At the motor tongue area the negative BP was followed by a negative slope (NS′) and a motor potential (MP), and at the sensory tongue area the positive BP and a positive reafferent potential (RAP) were seen but no NS′ and MP could be identified. In the other 4 patients, tongue protrusions elicited positive BP, NS′ and MP at the motor and sensory tongue area, and positive RAP at the sensory area. It was concluded that BPs, NS′ and MPs are mainly generated in the motor cortex involving the crown as well as the anterior bank of the central fissure. The sensory cortex (areas 3a and 3b) also participated in the generation of BPs but to a lesser degree. Different degree of involvement of these multiple generators most likely explains the interindividual variability of polarity and distribution of the MRPs. RAPS most likely arise from primary sensory areas 1 and 2. Brain potentials were also recorded at the motor (2 patients) and sensory (2 patients) language areas, but no specific language-related potentials could be identified.Evoked potentials to lip stimulation were investigated in 4 patients. In 3 patients, the responses at the sensory tongue area (P16, N21 and P30) had the same latency but opposite polarity to those at the motor tongue area. In the other patient, the responses (P16, N21 and P30) at the motor and sensory tongue areas were of the same polarity. The MRPs to tongue protrusions in those 4 patients revealed the same polarity relationship between the pre- and postcentral potentials. However, the maximal amplitude of evoked potentials and MRPs was seen at almost the same electrodes, suggesting that the main generators for these MRPs and evoked potentials must be located at contiguous areas in the anterior and posterior bank, respectively, of the central fissure.     

Influence of concurrent tactile stimulation on somatosensory evoked potentials following posterior tibial nerve stimulation in man

A topographical study was made of SEPs following stimulation of the right posterior tibial nerve at the ankle, with and without concurrent tactile stimulation of the soles of either foot or the palm of the right hand. Effects of the interfering stimulus were best demonstrated by subtracting the wave forms to derive ‘difference’ potentials.The majority of SEP components were significantly attenuated by tactile stimulation of the ipsilateral foot, and the difference wave form was of similar morphology to the control response. Components of opposite polarity peaking at 39 msec were consistent with the field of a cortical generator with dipolar properties, situated in the contralateral hemisphere just posterior to the vertex with the positive poles oriented towards the ipsilateral side. By analogy with median SEP findings, these potentials were believed to originate in the foot region of area 3b where neurones are mainly concerned with cutaneous sensory processing.When the tactile stimulus was applied to the contralateral foot, difference potentials maximally recorded just posterior to the vertex were of smaller amplitude but similar morphology to ipsilateral foot difference components. This suggested the possibility that input from the two lower extremities may converge at cortical or subcortical level, the effect being manifested in the response of certain neurones in area 3b. With both contralateral foot and ipsilateral hand stimulation, other difference potentials were present which suggested that there may be cortical regions responding to combinations of sensory stimuli applied to various parts of the body surface.     

Does the supplementary motor area play a part in modifying motor cortex reflexes?

Neuronal activity in the supplementary motor area was recorded from a monkey performing a trained motor task that required readiness for proper usage of sensory inputs. Thirty-two neurons exhibited activity changes, which supports the hypothesis that the SMA is part of the system involved in modulating responsiveness of the motor cortex to sensory inputs in association with learned movements.     

Dynamic Changes in Brain Activations and Functional Connectivity during Affectively Different Tactile Stimuli

In the present study, we compared brain activations produced by pleasant, neutral and unpleasant touch, to the anterior lateral surface of lower leg of human subjects. It was found that several brain regions, including the contralateral primary somatosensory area (SI), bilateral secondary somatosensory area (SII), as well as contralateral middle and posterior insula cortex were commonly activated under the three touch conditions. In addition, pleasant and unpleasant touch conditions shared a few brain regions including the contralateral posterior parietal cortex (PPC) and bilateral premotor cortex (PMC). Unpleasant touch specifically activated a set of pain-related brain regions such as contralateral supplementary motor area (SMA) and dorsal parts of bilateral anterior cingulated cortex, etc. Brain regions specifically activated by pleasant touch comprised bilateral lateral orbitofrontal cortex (OFC), posterior cingulate cortex (PCC), medial prefrontal cortex (mPFC), intraparietal cortex and left dorsal lateral prefrontal cortex (DLPFC). Using a novel functional connectivity model based on graph theory, we showed that a series of brain regions related to affectively different touch had significant functional connectivity during the resting state. Furthermore, it was found that such a network can be modulated between affectively different touch conditions.     

Differential Effects of Motor Efference Copies and Proprioceptive Information on Response Evaluation Processes

It is well-kown that sensory information influences the way we execute motor responses. However, less is known about if and how sensory and motor information are integrated in the subsequent process of response evaluation. We used a modified Simon Task to investigate how these streams of information are integrated in response evaluation processes, applying an in-depth neurophysiological analysis of event-related potentials (ERPs), time-frequency decomposition and sLORETA. The results show that response evaluation processes are differentially modulated by afferent proprioceptive information and efference copies. While the influence of proprioceptive information is mediated via oscillations in different frequency bands, efference copy based information about the motor execution is specifically mediated via oscillations in the theta frequency band. Stages of visual perception and attention were not modulated by the interaction of proprioception and motor efference copies. Brain areas modulated by the interactive effects of proprioceptive and efference copy based information included the middle frontal gyrus and the supplementary motor area (SMA), suggesting that these areas integrate sensory information for the purpose of response evaluation. The results show how motor response evaluation processes are modulated by information about both the execution and the location of a response.     

Do bimanual motor actions involve the dorsal premotor (PMd), cingulate (CMA) and posterior parietal (PPC) cortices? Comparison with primary and supplementary motor cortical areas

Single neuronal activity was recorded from the dorsal premotor cortex (PMd), the cingulate motor area (CMA) and the posterior parietal cortex (PPC) in two Macaca fascicularis trained to perform a delayed conditional sequence of coordinated pull and grasp movements. The monkey had to perform three types of trials instructed in a random manner: (i) bimanually, using the two hands in a coordinated sequence of movements; (ii) unimanually, using the left hand only; (iii) unimanually, using the right hand only. The aim of this study was first to assess the bilateral relationships of the three cortical areas for unimanual motor control. Second, to establish whether the three cortical areas contain units reflecting bimanual synergy. A total of 255 task-related neurons were recorded from the PMd, CMA and PPC, where most neurons exhibited a significant modulation of activity in both contralateral and ipsilateral unimanual trials (bilateral neurons: 85, 77 and 61%, respectively). Lower proportions of neurons in PMd (7%), CMA (16%) and PPC (6%) were active in unimanual contralateral trials, but not in unimanual ipsilateral trials. The reverse (modulation of activity in ipsilateral but not contralateral unimanual trials) represented 5% of neurons in PMd, 7% in CMA and 3% in PPC. When comparing unimanual and bimanual trials to search evidence for bimanual coordination, 57% of PMd task-related neurons were classified as bimanual, defined as units in which the activity observed in bimanual trials could not be predicted from that associated with unimanual trials when comparing the same events related to the same arm. The proportion of bimanual neurons in CMA (56%) was comparable to that found in PMd (55%), whereas PPC exhibited a higher proportion of bimanual neurons (74%). Furthermore, comparison of the present data with our previous results regarding the supplementary (SMA) and primary (M1) motor cortical areas shows that there is no statistically significant difference between PMd, CMA, SMA and M1 with respect to the proportions of bimanual neurons. Altogether, these results suggest that the five cortical areas PMd, CMA, PPC, SMA and M1 are participating to the control of sequential bimanually coordinated movements. Inter-limb coordination may thus be controlled by a widely distributed network including several cortical and sub-cortical areas.     

Do bimanual motor actions involve the dorsal premotor (PMd), cingulate (CMA) and posterior parietal (PPC) cortices? Comparison with primary and supplementary motor cortical areas

Single neuronal activity was recorded from the dorsal premotor cortex (PMd), the cingulate motor area (CMA) and the posterior parietal cortex (PPC) in two Macaca fascicularis trained to perform a delayed conditional sequence of coordinated pull and grasp movements. The monkey had to perform three types of trials instructed in a random manner: (i) bimanually, using the two hands in a coordinated sequence of movements; (ii) unimanually, using the left hand only; (iii) unimanually, using the right hand only. The aim of this study was first to assess the bilateral relationships of the three cortical areas for unimanual motor control. Second, to establish whether the three cortical areas contain units reflecting bimanual synergy. A total of 255 task-related neurons were recorded from the PMd, CMA and PPC, where most neurons exhibited a significant modulation of activity in both contralateral and ipsilateral unimanual trials (bilateral neurons: 85, 77 and 61%, respectively). Lower proportions of neurons in PMd (7%), CMA (16%) and PPC (6%) were active in unimanual contralateral trials, but not in unimanual ipsilateral trials. The reverse (modulation of activity in ipsilateral but not contralateral unimanual trials) represented 5% of neurons in PMd, 7% in CMA and 3% in PPC. When comparing unimanual and bimanual trials to search evidence for bimanual coordination, 57% of PMd task-related neurons were classified as bimanual, defined as units in which the activity observed in bimanual trials could not be predicted from that associated with unimanual trials when comparing the same events related to the same arm. The proportion of bimanual neurons in CMA (56%) was comparable to that found in PMd (55%), whereas PPC exhibited a higher proportion of bimanual neurons (74%). Furthermore, comparison of the present data with our previous results regarding the supplementary (SMA) and primary (M1) motor cortical areas shows that there is no statistically significant difference between PMd, CMA, SMA and M1 with respect to the proportions of bimanual neurons. Altogether, these results suggest that the five cortical areas PMd, CMA, PPC, SMA and M1 are participating to the control of sequential bimanually coordinated movements. Inter-limb coordination may thus be controlled by a widely distributed network including several cortical and sub-cortical areas.     

Scalp topography and dipolar source modelling of potentials evoked by CO2 laser stimulation of the hand

CO₂ laser evoked potentials to hand stimulation recorded using a scalp 19-channel montage in 11 normal subjects consistently showed early N1/P1 dipolar field distribution peaking at a mean latency of 159 ms. The N1 negativity was distributed in the temporoparietal region contralateral to stimulation and the P1 positivity in the frontal region. The N1/P1 response was followed by 3 distinct components: (1) N2a reaching its maximal amplitude at the vertex and ipsilaterally to the stimulated hand, (2) N2b mostly distributed in the frontal region, and (3) P2 with a mid-central topography. Brain electrical source analysis showed that this sequence was explained, with a residual variance below 5%, by a model including two dipoles in the upper bank of the Sylvian fissure of each hemisphere, a frontal dipole close to the midline, and two anterior medial temporal dipoles, thus suggesting a sequential activation of the two second somatosensory areas, anterior cingulate gyrus and the amygdalar nuclei or the hippocampal formations, respectively. This model fitted well with the scalp field topography of grand average responses to stimulation of left and right hand obtained across all subjects as well as when applied to individual data. Our findings suggest that the second somatosensory area contralateral to the stimulation is the first involved in the building of pain-related responses, followed by ipsilateral second somatosensory area and limbic areas receiving noxious inputs from the periphery.     

Serial recording of sensory,corticomotor, and brainstem-derived motor evoked potentials in the rat

A method is presented for serial recording of corticomotor evoked potentials (CMEPs), brainstem-derived motor evoked potentials (BMEPs), and somatosensory evoked potentials (SEPs) via permanently implanted cranial screws. One screw was positioned posterior to lambda (posterior screw), and two screws were positioned over the cortical hind limb areas (cortical screws). SEPs were elicited by stimulation of the hind paw and recorded from the contralateral cortex. BMEPs were stimulated via the posterior screw and recorded from both hind limbs, whereas CMEPs were elicited by repeated bipolar stimulation of the cortex and recorded from the contralateral hind limb. BMEPs and CMEPs differed in several points and can be considered as completely separate motor evoked potentials. While BMEPs consisted of a prominent negative peak with short latency (5–7.5 ms), CMEPs were represented by polyphasic signals with long latencies (17–22 ms). The cortical origin of the CMEPs was confirmed by transecting the corticospinal tracts, which abolished the CMEPs but spared the BMEPs. SEPs consisted of three consecutive peaks with mean latencies of the initial peak ranging between 15 and 17 ms. Dorsal column transection also abolished SEPs. In healthy rats, all three signals were recorded for six consecutive weeks. Signal parameters did not change significantly within this observation period. Rats tolerated the screws and the repeated measurements very well and no negative affect on animal behavior was noted. Thus, this method allows serial recording of SEPs, CMEPs, and BMEPs in chronic rat models.     

Serial recording of sensory, corticomotor, and brainstem-derived motor evoked potentials in the rat

A method is presented for serial recording of corticomotor evoked potentials (CMEPs), brainstem-derived motor evoked potentials (BMEPs), and somatosensory evoked potentials (SEPs) via permanently implanted cranial screws. One screw was positioned posterior to lambda (posterior screw), and two screws were positioned over the cortical hind limb areas (cortical screws). SEPs were elicited by stimulation of the hind paw and recorded from the contralateral cortex. BMEPs were stimulated via the posterior screw and recorded from both hind limbs, whereas CMEPs were elicited by repeated bipolar stimulation of the cortex and recorded from the contralateral hind limb. BMEPs and CMEPs differed in several points and can be considered as completely separate motor evoked potentials. While BMEPs consisted of a prominent negative peak with short latency (5-7.5 ms), CMEPs were represented by polyphasic signals with long latencies (17-22 ms). The cortical origin of the CMEPs was confirmed by transecting the corticospinal tracts, which abolished the CMEPs but spared the BMEPs. SEPs consisted of three consecutive peaks with mean latencies of the initial peak ranging between 15 and 17 ms. Dorsal column transection also abolished SEPs. In healthy rats, all three signals were recorded for six consecutive weeks. Signal parameters did not change significantly within this observation period. Rats tolerated the screws and the repeated measurements very well and no negative affect on animal behavior was noted. Thus, this method allows serial recording of SEPs, CMEPs, and BMEPs in chronic rat models.     

Neuronal activity in the primate supplementary motor area and the primary motor cortex in relation to spatio-temporal bimanual coordination

Single neuronal activity was recorded from the supplementary motor area (SMA-proper and pre-SMA) and primary motor cortex (M1) in two Macaca fascicularis trained to perform a delayed conditional sequence of coordinated bimanual pull and grasp movements. The behavioural paradigm was designed to distinguish neuronal activity associated with bimanual coordination from that related to a comparable motor sequence but executed unimanually (left or right arm only). The bimanual and unimanual trials were instructed in a random order by a visual cue. Following the cue, there was a waiting period until presentation of a "go-signal", signalling the monkey to perform the instructed movement. A total of 143 task-related neurons were recorded from the SMA (SMA-proper, 62; pre-SMA, 81). Most SMA units (87%) were active in both unimanual contralateral and unimanual ipsilateral trials (bilateral neurons), whereas 9% of units were active only in unimanual contralateral trials and 3% were active only in unimanual ipsilateral trials. Forty-eight per cent of SMA task-related units were classified as bimanual, defined as neurons in which the activity observed in bimanual trials could not be predicted from that associated with unimanual trials when comparing the same events related to the same arm. For direct comparison, 527 neurons were recorded from M1 in the same monkeys performing the same tasks. The comparison showed that M1 contains significantly less bilateral neurons (75%) than the SMA, whereas the reverse was observed for contralateral neurons (22% in M1). The proportion of M1 bimanual cells (53%) was not statistically different from that observed in the SMA. The results suggest that both the SMA and M1 may contribute to the control of sequential bimanual coordinated movements. Interlimb coordination may then take place in a distributed network including at least the SMA and M1, but the contribution of other cortical and subcortical areas such as cingulate motor cortex and basal ganglia remains to be investigated.     

SEP topographies elicited by innocuous and noxious sural nerve stimulation. III. Dipole source localization analysis

The dipole source localization method was used to determine which of the brain areas known to be involved in somatosensation are the best candidate generators of the somatosensory evoked potential evoked by sural nerve stimulation. The ipsilateral central negativity and contralateral frontal positivity which occurred between 58 and 90 msec post stimulus (stable period 1) were best represented by a single source located in the primary somatosensory cortex (SI). The symmetrical central negativity and bilateral frontal positivity which occurred between 92 and 120 msec post stimulus (stable period 2) was best represented by 3 sources. One of these sources was located in SI and the other 2 were located bilaterally in either the frontal operculum or near the second somatosensory cortex (SII). The widespread negativity whose minimum was located in the contralateral fronto-temporal region and which occurred between 135 and 157 msec post stimulus (stable period 3) was also best represented by 3 sources. Two of these sources may be located bilaterally in the hippocampus. We cannot, however, eliminate the possibility that multiple sources in the cortex overlying the hippocampus (e.g., SII and frontal cortex) are responsible for these potentials. At innocuous stimulus levels the third source for stable period 3 was located near the vertex, possibly involving the supplementary motor cortex, whereas at noxious levels this source appears to be located in the cingulate cortex. We were unable to achieve any convincing source localization for the widespread positivity which occurred between 178 and 339 msec post stimulus (stable periods 4–6). Available evidence suggests that more sources were active during this interval than the three we could reliably test under these conditions.     

Cerebral cortical representation of reflexive and volitional swallowing in humans

The purpose of this study was to compare cerebral cortical representation of experimentally induced reflexive swallow with that of volitional swallow. Eight asymptomatic adults (24-27 yr) were studied by a single-trial functional magnetic resonance imaging technique. Reflexive swallowing showed bilateral activity concentrated to the primary sensory/motor regions. Volitional swallowing was represented bilaterally in the insula, prefrontal, cingulate, and parietooccipital regions in addition to the primary sensory/motor cortex. Intrasubject comparison showed that the total volume of activity during volitional swallowing was significantly larger than that activated during reflexive swallows in either hemisphere (P < 0.001). For volitional swallowing, the primary sensory/motor region contained the largest and the insular region the smallest volumes of activation in both hemispheres, and the total activated volume in the right hemisphere was significantly larger compared with the left (P < 0.05). Intersubject comparison showed significant variability in the volume of activity in each of the four volitional swallowing cortical regions. We conclude that reflexive swallow is represented in the primary sensory/motor cortex and that volitional swallow is represented in multiple regions, including the primary sensory/motor cortex, insular, prefrontal/cingulate gyrus, and cuneus and precuneus region. Non-sensory/motor regions activated during volitional swallow may represent swallow-related intent and planning and possibly urge.     

大鼠脊髓诱发电位与脊髓损伤的关系——Ⅲ.脊髓局部损毁后电位的变化及电位来源分析

本文描述了大鼠脊髓L_1节段后柱、后索、侧索和前角的诱发电位及其损伤后的变化,并观察了切断L_4、L_5脊神经背、腹根与横断高位颈髓对电位的影响,以进行行电位来源分析。结果可见,上述四个区域的诱发电位基本由早反应三相波和晚反应组成。分别电解损毁这些部位后,电位波幅均普遍降低,晚期反应较早反应降低明显。后柱或后索受损对电位影响最大。局部损毁后可见L_1及T_(13)水平的硬膜上电位改变明显,尤其晚反应减弱、波峰平坦。反应时值与潜伏时未见明显改变。切断L_4脊神经背、腹根后、电位基本消失。去大脑对电位未见明显影响。结果表明,刺激坐骨神经诱发的脊髓电位起源于低位腰段传入神经和脊髓内多通路的兴奋传导,在一定程度上受腹根逆行活动的影响,与大脑及脊髓下行传导束活动无直接联系。脊髓诱发电位的幅度与波形改变可作为脊髓损伤的判断指标之一。     

Levodopa Effects on Hand and Speech Movements in Patients with Parkinson’s Disease: A fMRI Study

Levodopa (L-dopa) effects on the cardinal and axial symptoms of Parkinson’s disease (PD) differ greatly, leading to therapeutic challenges for managing the disabilities in this patient’s population. In this context, we studied the cerebral networks associated with the production of a unilateral hand movement, speech production, and a task combining both tasks in 12 individuals with PD, both off and on levodopa (L-dopa). Unilateral hand movements in the off medication state elicited brain activations in motor regions (primary motor cortex, supplementary motor area, premotor cortex, cerebellum), as well as additional areas (anterior cingulate, putamen, associative parietal areas); following L-dopa administration, the brain activation profile was globally reduced, highlighting activations in the parietal and posterior cingulate cortices. For the speech production task, brain activation patterns were similar with and without medication, including the orofacial primary motor cortex (M1), the primary somatosensory cortex and the cerebellar hemispheres bilaterally, as well as the left- premotor, anterior cingulate and supramarginal cortices. For the combined task off L-dopa, the cerebral activation profile was restricted to the right cerebellum (hand movement), reflecting the difficulty in performing two movements simultaneously in PD. Under L-dopa, the brain activation profile of the combined task involved a larger pattern, including additional fronto-parietal activations, without reaching the sum of the areas activated during the simple hand and speech tasks separately. Our results question both the role of the basal ganglia system in speech production and the modulation of task-dependent cerebral networks by dopaminergic treatment.     

Effect of electrical stimulation of the hippocampus and neocortex on cingulate cortical neurons in rabbits

Extracellular unit activity of the anterior and posterior zones of the cingulate cortex and also of the anterior and posterior cortical association areas was analyzed in unanesthetized rabbits. In the posterior zone considerably more cells (60%) responded to hippocampal stimulation than in the anterior zone (18%). In 43% of these cells in the posterior zone but only in 5% in the anterior zone, the responses followed the frequency of stimulation. Unit responses in the posterior zone could be divided into two discrete groups: those with short (12.3 ± 6.5 msec) and those with long (50.2 ± 10.0 msec) latent periods. Inhibitory phenomena also were well marked during hippocampal stimulation. More than one-third of cells of the cingulate cortex responded to stimulation of the posterior association zones by spikes which followed the stimulus, and by subsequent inhibition. Responses of this kind to stimulation of the anterior association zones were found in only a few cells in the anterior zone of the cingulate cortex. The results are discussed in the light of data from morphological investigations relating to connections between these structures.Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino-on-Oka. Translated from Neirofiziologiya, Vol. 14, No. 3, pp. 270–277, May–June, 1982.     

Response properties of diencephalic neurons to visual, acoustic and hydrodynamic stimulation in the goldfish, Carassius auratus

We studied the responses to sensory stimulation of three diencephalic areas, the central posterior nucleus of the dorsal thalamus, the anterior tuberal nucleus of the hypothalamus, and the preglomerular complex. Units sensitive to acoustic (500 Hz tone burst), hydrodynamic (25 Hz dipole stimulus) and visual (640 nm light flash) stimuli were found in both the central posterior and anterior tuberal nucleus. In contrast, unit responses or large robust evoked potentials confined to the preglomerular complex were not found. In the central posterior nucleus, most units were unimodal. Many units responded exclusively to visual stimulation and exhibited a variety of temporal response patterns to light stimuli. In the anterior tuberal nucleus of the hypothalamus, most units responded to more than one modality and showed a stronger response decrement to stimulus repetitions than units in the central posterior nucleus. Our data suggest that units in the central posterior nucleus are primarily involved in the unimodal processing of sensory information whereas units in the anterior tuberal nucleus of the hypothalamus may be involved in multisensory integration.     

Analysis of evoked discharges in hypothalamic neurons to somatosensory,acoustic, and photic stimulation

Single unit activity in the supramammillary, mammillary, and anterior hypothalamic areas in response to acoustic, photic, and sciatic nerve stimulation was recorded in cats anesthetized with chloralose and immobilized with succinylcholine. In response to sensory stimulation the spontaneous firing rate was increased or decreased, and silent neurons were activated. Evoked potentials of the silent neurons had the shortest latent period to acoustic and somatosensory stimulation (15 msec), and rather longer to photic stimulation (30 msec); in some cases their latent period was 200 msec. Histograms of interspike interval distribution showed a maximum for intervals of up to 50 msec. Histograms of spike distribution relative to the beginning of stimulation showed maximal density between 100 and 200 msec. A high degree of convergence of excitation was found on units of the anterior as well as the posterior hypothalamus. Unit responses in the hypothalamus to sensory stimuli of all three modalities are regarded as being of secondary, nonspecific type.L. A. Orbeli Institute of Physiology, Academy of Sciences of the Armenian SSR, Erevan. Translated from Neirofiziologiya, Vol. 3, No. 6, pp. 592–598, November–December, 1971.     

Mirroring Pain in the Brain: Emotional Expression versus Motor Imitation

Perception of pain in others via facial expressions has been shown to involve brain areas responsive to self-pain, biological motion, as well as both performed and observed motor actions. Here, we investigated the involvement of these different regions during emotional and motor mirroring of pain expressions using a two-task paradigm, and including both observation and execution of the expressions. BOLD responses were measured as subjects watched video clips showing different intensities of pain expression and, after a variable delay, either expressed the amount of pain they perceived in the clips (pain task), or imitated the facial movements (movement task). In the pain task condition, pain coding involved overlapping activation across observation and execution in the anterior cingulate cortex, supplementary motor area, inferior frontal gyrus/anterior insula, and the inferior parietal lobule, and a pain-related increase (pain vs. neutral) in the anterior cingulate cortex/supplementary motor area, the right inferior frontal gyrus, and the postcentral gyrus. The ‘mirroring’ response was stronger in the inferior frontal gyrus and middle temporal gyrus/superior temporal sulcus during the pain task, and stronger in the inferior parietal lobule in the movement task. These results strongly suggest that while motor mirroring may contribute to the perception of pain expressions in others, interpreting these expressions in terms of pain content draws more heavily on networks involved in the perception of affective meaning.