共查询到20条相似文献,搜索用时 62 毫秒
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小黑杨花粉植株的诱导 总被引:6,自引:2,他引:4
1 植物名称 小黑杨 (Populussimonii×P .ni gra)。2 材料类别 花药 (anther)。3 培养条件 ( 1 )诱导愈伤组织培养基 :MS 2 ,4 D 4mg·L- 1 (单位下同 ) KT 2。 ( 2 )愈伤组织分化培养基 :MS 6 BA 1 NAA 0 .5。当愈伤组织分化出嫩芽后 ,将芽切下转入MH 6 BA0 .5 NAA 0 .1培养基上 ,其中MH培养基为MS的大量元素和有机物 ,MH培养基的微量元素 ,再加叶酸 (folacin)及生物素 (biotin)各 0 .1。( 3)生根培养基 :1 /2MS IBA 0 .4。上述培养基均添加 2 %… 相似文献
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何首乌愈伤组织诱导和植株再生 总被引:8,自引:0,他引:8
1 植物名称 何首乌 (Polygonummultiflorum)。2 材料类别 蒙山阳坡路旁野生植株上的嫩芽。3 培养条件 诱导愈伤组织培养基 :(1 )MS 6 BA 1mg·L-1(单位下同 ) NAA 1 ;(2 )MS 6 BA 2 NAA 1 ;(3) 1 /2MS NAA 0 .3;(4) 1 /2MS 2 ,4 D 0 .1 ;(5 ) 1 /2MS 2 ,4 D0 .3;(6 ) 1 /2MS 2 ,4 D 1 ;(7)MS 6 BA 3 2 ,4 D 1。芽分化培养基 :(8)MS 6 BA 1 ;(9)MS 6 BA 2 ;(1 0 )MS 6 BA 5 NAA 0 .1 ;(1 1 )MS 6 BA 2 NAA 0 .4;(1 2 … 相似文献
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星花绣线菊的组织培养及快速繁殖 总被引:11,自引:3,他引:8
1 植物名称 星花绣线菊 (Spiraeajaponicavar.stelleris)。2 材料类别 茎尖、茎段、叶片、叶柄。3 培养条件 ( 1 )愈伤组织诱导培养基 :MS 2 ,4 D 2 .0mg·L- 1 (单位下同 ) KT 0 .3;( 2 )愈伤组织继代培养基 :6,7 V 2 ,4 D 2 .0 KT 0 .2 5 NAA 1 .0 LH 2 0 0 0 ;( 3)芽诱导培养基 :MS 6 BA 2 .0 NAA 0 .1 ;( 4 )芽增殖培养基 :MS 6 BA0 .2 5 NAA 0 .1 ;( 5 )生根培养基 :1 /2MS 6 BA0 .2 5 NAA 0 .5。上述培养基的蔗糖含量为 3% ,琼脂为 0 .7% ,… 相似文献
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1 植物名称 分蘖洋葱 (Alliumcepavar multi plcansBaileysyn var AgrogatumDon)。2 材料类别 鳞茎茎尖。3 培养条件 基本培养基为MS。愈伤组织诱导培养基 :(1 )MS KT 0 5mg·L- 1 (单位下同 ) 2 ,4 D 2 0。愈伤组织分化培养基 :(2 )MS 6 BA3 0 NAA 1 0 ;(3 ) :MS 6 BA 0 4 NAA 0 1。生根培养基 :(4) 1 /2MS IBA1 5 NAA 0 0 1。上述培养基均加蔗糖 3 % ,琼脂 0 8% ,调pH值为 5 7~5 8,1 2 1℃高温高压灭菌 1 4min。培养温… 相似文献
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有棱丝瓜的组织培养及快速繁殖 总被引:1,自引:0,他引:1
1 植物名称 有棱丝瓜 (Luffaacutangula)品种“绿旺”。2 材料类别 真叶、下胚轴、上胚轴及胚芽。3 培养条件 基本培养基为 ( 1 )MS。诱导愈伤组织培养基 :( 2 )MS 6 BA 5mg·L- 1 (单位下同 ) NAA 0 .5。诱导胚性愈伤组织及分化培养基 :( 3)MS 6 BA 2 IAA 0 .2 IBA 0 .2。胚性愈伤组织继代增殖培养基 :( 4 )MS 6 BA 4 KT0 .5 NAA 1。生根培养基 :( 5 ) 1 /2MS IAA 0 .2。以上培养基 pH均为 5 .8。糖浓度在培养基 ( 1 )、( 2 )、( 4 )、( 5 )中为 3% ,在培养基 ( 3)… 相似文献
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1 植物名称 地被菊 (Dendranthemagrandiflorum)品种早小菊。2 材料类别 花蕾。3 培养条件 以MS为基本培养基。愈伤组织诱导及分化培养基 :(1 )MS 6 BA 2mg·L-1(单位下同 ) NAA 0 .2。继代培养基 :(2 )MS NAA 0 .2 ;(3 )MS 6 BA 0 .0 2 NAA 0 .2 ;(4)MS 6 BA 2 NAA 0 .2。生根培养基 :(5 ) 1 2MS 6 BA 0 .0 2 IBA 1 ;(6 ) 1 2MS IBA 1 ;(7) 1 2MS IBA 2 ;(8) 1 2MS IBA 3。上述培养基均添加 2 %蔗糖 ,0 .7%琼脂。培养基pH为 6 … 相似文献
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1 植物名称 黎豆 (Stizolobiumcochinchinensis) ,别名猫豆、狗爪豆。2 材料类别 无菌种子苗。3 培养条件 基本培养基为MS。种子萌发及壮苗培养基 :(1 )MS Vc 0 .5mg·L-1(单位下同 ) 0 .1 %活性炭 ;诱导丛芽培养基 :(2 )MS 6 BAl.0 NAA 0 .1 Vc 0 .5 ;诱导愈伤组织培养基 :(3 )MS 6 BA 0 .5 NAA 0 .0 5 ,(4)MS 2 ,4 D 0 .5 ,(5 )MS 2 ,4 D 1 .0 ,(6 )MS 2 ,4 D 2 .0 ;分化培养基 :(7)MS 6 A3 .0 Vc 0 .5 NAA 0 .0 5 ;芽体生长及诱导生根培养基… 相似文献
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In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980. 相似文献
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N. P. Vesselkin Yu. V. Natochin 《Journal of Evolutionary Biochemistry and Physiology》2010,46(6):592-603
Evolution of living organisms is closely connected with evolution of structure of the system of regulations and its mechanisms.
The functional ground of regulations is chemical signalization. As early as in unicellular organisms there is a set of signal
mechanisms providing their life activity and orientation in space and time. Subsequent evolution of ways of chemical signalization
followed the way of development of delivery pathways of chemical signal and development of mechanisms of its regulation. The
mechanism of chemical regulation of the signal interaction is discussed by the example of the specialized system of transduction
of signal from neuron to neuron, of effect of hormone on the epithelial cell and modulation of this effect. These mechanisms
are considered as the most important ways of the fine and precise adaptation of chemical signalization underlying functioning
of physiological systems and organs of the living organism 相似文献
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