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Ye Zhang Meng Lan Jin‐Peng Lü Jing‐Feng Li Kai‐Yue Zhang Hui Zhi Hui Zhang Jia‐Ming Sun 《化学与生物多样性》2020,17(1)
Chroogomphus rutilus is a rare fungal species that grows under pine trees and is now widely used as a functional food and pharmaceutical product. However, the chemical constituents and biological activities of Chroogomphus rutilus have been relatively limited. The present study aimed at determining the total polyphenols and flavonoids contents, biological activities and main phenolic compounds of Chroogomphus rutilus from different geographical origins at the stipe and pileus. The results suggested that Chroogomphus rutilus polyphenol extracts revealed a higher antioxidant, anti‐inflammatory, and cytotoxic activities, and there were significant differences between samples from different locations and regions. Correlation analysis showed that the contents of total polyphenols and flavonoids were significantly correlated with antioxidant and anti‐inflammatory activities. However, only the content of total flavonoids was significantly correlated with cytotoxicity, which means that the cytotoxicity of Chroogomphus rutilus polyphenol extracts may be regulated by flavonoids or other compounds. HPLC‐DAD analysis revealed that the main phenolic compound was protocatechuic acid, followed by baicalin, p‐hydroxyphenylacetic acid and p‐hydroxybenzoic acid, but comparing with the pileus extracts, the stipe extracts can be considered as a higher concentration of phenolic compounds. Therefore, antioxidant, anti‐inflammatory and cytotoxic activities of Chroogomphus rutilus polyphenol extracts could be due to the identified compounds. This study investigated a deep knowledge about the constituents and activities of Chroogomphus rutilus and provided the reference for its application in food and pharmaceutical. 相似文献
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Microalgae have been investigated for their ability to produce metabolites that exhibit antibacterial activity. However, as research on antibacterial activity progresses, the effect of microalgal extracts on mammalian cells needs to be also assessed. The in vitro effect of microalgal extracts with demonstrated antibacterial activity against the human opportunistic pathogen Staphylococcus aureus was examined on the viability of non‐malignant (MCF10A and 184B5 cells) and malignant human cell lines (A2780 and MCF7). Direct cell counts indicated that the MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) proliferation assay was found to under/overestimate cell viability when specific microalgal extracts and/or concentrations were tested. From direct cell counts, the viability of non‐malignant cells was not reduced after exposure to the extracts, whereas the viability of malignant cells was affected by specific microalgal extracts and concentrations. The results suggest that green microalgae are worthy of further investigation as potential sources of antibiotics, since they did not show a negative effect on the non‐malignant cell lines, MCF10A and 184B5. Additional studies should evaluate the compounds responsible for the anti‐proliferative activity of specific microalgal extracts observed on the malignant cells A2780 and MCF7. 相似文献
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A chemiluminescence (CL) assay for the determination of antioxidant capacity (AOC) has been optimized and applied to analyses of herbal extracts in the present study. The optimal concentrations of reagents (luminol, H2O2, horseradish peroxidase) have been determined, as well as the optimal reaction conditions (wavelength, pH, temperature, sample volume). All of the measurements were performed at the emission maximum of the oxidized form of luminol (425 nm). The optimal concentrations of the reagents were determined as follows: 1.6 mmol/L luminol, 7.5 mmol/L H2O2 and 0.14 U/mL horseradish peroxidase activity in the reaction mixture. Analyses were carried out in phosphate buffer, pH 7.4, at room temperature. With the optimized CL assay, the AOCs of various water and methanol herbal extracts were determined (dog rose hips, plantain leaves and coltsfoot and thyme flowers) and the results were compared to those obtained by other classical methods for the evaluation of antioxidants. Strong correlations (r > 0.9) with the Folin–Ciocalteau assay and the 2,2‐diphenyl‐1‐picrylhydrazyl radical (DPPH)● assay are confirmed, although there is no correlation between AOC and the concentration of ascorbic acid in the samples analysed. This optimized CL assay is simple, rapid and reliable, and it represents a good alternative to classical methods (Folin–Ciocalteau, DPPH●) for the determination of AOC of herbal extracts and other food samples. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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Jahir Antonio Barajas-Ramírez Angel Humberto Cabrera-Ramírez Victoria Guadalupe Aguilar-Raymundo 《化学与生物多样性》2023,20(1):e202200834
In Mexico, plants are commonly used to alleviate various ailments, including controlling some chronic degenerative diseases through the regular consumption of decoctions, infusions, and teas. However, there is little scientific evidence consolidating traditional medicine within health systems. Therefore, this work determined the phytochemical profile of the most used plants to treat various ailments (Cedro rojo, Cancerina, Ortiguilla, Hierba de la golondrina, Hierba de arlomo) and their general consumption as infusions. Aqueous and ethanolic extracts were generated, while the phytochemical compound content in the extracts obtained was quantified. The results indicate that the ethanolic extracts showed the highest phenolic compound and tannin content, with the highest contents for Cedro rojo (831.04 mg L−1) and Cancerina (864.80 mg L−1). The antioxidant activity was also determined, and a significant difference was observed (p<0.05). The extracts with the highest antioxidant capacity were the ethanolic extracts ranging from 250 to 907 μMET mL−1, while the aqueous extracts ranged from 112 to 390 μMET mL−1. The compounds identified by high-performance liquid chromatography characterization on the aqueous extracts highlighted the presence of chlorogenic acid>cinnamic acid>quercetin. In ethanolic extracts, the presence of chlorogenic acid>cinnamic acid>quercetin>gallic acid>ferulic acid>coumaric acid was highlighted. The correlation between bioactive compounds, type of extract, and antioxidant activity suggests a significant affinity of these phytochemical compounds for the ethanol solvent. The results indicate that these plants are good sources of antioxidant phenolics and can be incorporated for use as functional beverages. However, more studies are needed to corroborate their beneficial effect. 相似文献
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氢气在能源领域的优势已日渐凸显,其在医学领域同样是一种清洁、高效、经济的治疗手段。氢医学领域主要包括氢气对疾病的基础研究和临床研究,如氢气的使用方法、剂量、对健康的促进作用、对疾病的治疗效果以及作用机理等。氢分子可以清除羟基自由基和过氧亚硝酸盐,对氧化应激和炎症相关疾病具有显著的治疗效果,同时其作为一种内源性气体,无毒无害,对人体不会造成不良反应。通过直接摄入和控制释放等方式,可以实现对脑和神经系统疾病、心血管疾病、糖尿病和癌症等疾病的靶向治疗。介绍了释放氢气的不同方式及其在医学领域的研究进展,并对氢医学的科学和实践问题进行了展望,以期为氢气在生物医学领域的应用研究提供参考。 相似文献
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Ferredoxin-sulfite reductase (Fd-SiR) [hydrogen-sulfide: ferredoxin oxidoreductase, EC 1.8.7.1] from spinach leaves has been purified to homogeneity by a new procedure. Subunit analysis by sodium dodecyl sulfate gel electrophoresis yielded a single protein band with a molecular weight of 71,000. Gel electrophoresis in non-denaturing media at different acrylamide concentrations gave a molecular weight of 270,000, suggesting that the native enzyme was composed of four identical subunits. In the presence of 0.2 m sodium chloride, however, gel filtration produced a value of 136,000, indicating the presence of dimer in this ionic environment. A plot of substrate (sulfite) concentration versus enzymatic (Fd-SiR) activity yielded a sigmoidal curve, giving a Hill coefficient (n?) of 2.1. Purified Fd-SiR, in the oxidized form, had absorption maxima at 279, 385, 588 and 714nm. Thus the enzyme has the property of a siroheme-containing protein. 相似文献
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树脂酸及其衍生物的生物活性 总被引：1，自引：0，他引：1
树脂酸是松香的主要组分,在工业上具有广泛的应用。近年来,研究表明树脂酸及其衍生物具有重要的生物学功能,包括抗氧化、消炎等活性。本文综述了这些化合物的生物活性。 相似文献
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Hnia Chograni Leila Riahi Yosr Zaouali Mohamed Boussaid 《African Journal of Ecology》2013,51(2):343-347
Leaves and flowers from Tunisian Globularia alypum were assessed for their phenolic and flavonoid contents and antioxidant activity. Phenolic (15.5–22.30 mg GAE g?1 DW) and flavonoid (3.63–4.72 mg RE g?1 DW) contents varied according to plant part. Globularia alypum leaves exhibited the highest phenol and flavonoid contents, and displayed the highest antioxidant activity, based on radical‐scavenging activity (295 μmol TEAC g?1 DW) and ferric reducing power (24.27 mmol Fe2+/l). The obtained results demonstrated that some of the isolated compounds play an important role for the antioxidant activity of G. alypum and give a scientific basis to the use of this plant in traditional medicine. The methanolic extract of G. alypum could be considered as a source of potential antioxidants and will promote the reasonable usage of this plant in food technology and processing as well as for medical use. 相似文献
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为有效开发利用沙苑子,探究其抗氧化特性为深加工提供理论依据和经验参数,本文主要从沙苑子酚类提取物的总还原能力,对超氧阴离子自由基和羟基自由基的清除能力,及对Fe2+诱导的脂质过氧化的抑制作用等四个方面进行了研究并比较了三种不同沙苑子酚类提取物的抗氧化性能。结果表明沙苑子酚类提取物均具有一定的抗氧化能力,在实验浓度范围内呈现一定的量效关系。 相似文献
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甘草提取物的体外抗氧化活性 总被引：1，自引：1，他引：1
为研究甘草提取物的体外抗氧化作用,采用60%乙醇回流提取甘草(LF),甘草粗提物(LF0)通过AB-8吸附树脂纯化制取甘草精提物(LF1),对LF0和LF1主要活性成分的含量进行测定。分别采用DPPH法、2-脱氧-D-核糖法和NBT-NADH-PMS体系、β-胡萝卜素-亚油酸体系测定LF0和LF1对1,1-二苯基-2-苦苯肼自由基(DPPH.)、羟自由基(OH.)、超氧阴离子自由基(.O-2)的清除能力以及LF0和LF1的总抗氧化能力;通过对LF0和LF1还原能力的测定分析LF0和LF1的抗氧化活性。研究结果显示,LF0和LF1能有效清除.O2-、DPPH.和OH.,具有良好的总抗氧化能力和还原能力,且LF1的作用比LF0好。 相似文献
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The fatty acid, sterol and chlorophyll composition of the calcified, unicellular alga Thoracosphaera heimii (Lohmann) Kamptner are reported. The presence of 4,23,24-termethyl-5α-cholest-22E-en-3β-ol (dinosterol), 4,23,24-trimethyl-5α-cholest-22E-en-3-one (dinosterone) and the predominance of C18, C20 and C22 unsaturated fatty acids, including the acid 18:5ω3, indicates that T. heimii is a dinoflagellate. The fatty acid: sterol ratio (1.3), is typical of dinoflagellates. The geochemical significance of dinosterone, the high relative concentration of 4-desmethyl-5α-stanols and the role of 23-methyl-5α-cholest-22E-en-3β-ol in the biosynthesis of dinosterol in T. heimii are also discussed. 相似文献
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Azza A. M. AlKhathami Hosam A. Saad Fareed A. Fareed Eman S. El-Shafey Eslam S. Elsherbiny Mamdouh R. El Nahas Mohamed R. E. Aly 《化学与生物多样性》2023,20(2):e202200670
We previously reported that synthetic oleoyl chalcones had a favorable effect to alleviate metabolic consequences of obesity in male SD rats. In this work, we prepared and characterized by spectroscopic tools, a set of six oleoyl chalcones ( 5a–c , 10 and 11a,b ). The comparative effects of the previously prepared oleoyl chalcones and their new synthetic analogs on metabolic and histological changes in obese male SD rats were studied. It was found that the oleoyl chalcones IIIa and IV were the best in improving many metabolic parameters, e. g., FBG, FI, ISI, TG, and total cholesterol. They cured systemic inflammation, through inhibition of the TNF-α and induction of adiponectin production. Moreover, chalcones IIIa and IV alleviated the oxidative stress accompanying obesity through the induction of the antioxidant enzymes GPX, SOD and CAT besides, GSH. Interestingly, chalcones IIIa and IV exerted hepatoprotective potency and ameliorated the manifestations of NAFLD via inhibition of apoptosis and induction of autophagy of hepatic cells. In conclusion, the oleoyl chalcones IIIa and IV were the most effective candidates among the series of synthetic chalcones in correcting body weight and the consequent metabolic and histological changes in adiposity. 相似文献
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《Journal of enzyme inhibition and medicinal chemistry》2013,28(3):440-444
Conferin (1), a new isoflavone, has been isolated from the ethyl acetate soluble fraction of Caragana conferta Benth. along with seven known compounds, namely biochanin A (2), p-hydroxybenzoic acid (3), 3,5- dimethoxybenzoic acid (4), ursolic acid (5), erythrodiol (6), pinoresinol (7), and syringresinol (8), reported for the first time from this species. The structure of the new isoflavone was deduced on the basis of spectroscopic studies. Compounds 1 and 2 were investigated for biological activities and showed significant anti-inflammatory activity in carrageenan induced paw edema of rats. Evaluation of antioxidant activity by the radical scavenging method indicated that compound 1 is a potent antioxidant while 2 is moderately active. It was also shown that the reducing capability of compound 2 was remarkably increased in a concentration dependent manner as compared to 1. Compound 1 showed moderate inhibitory activity against the enzyme lipoxygenase, while 2 showed weak activity. 相似文献
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Khashayar Afshari Nazgol-Sadat Haddadi Arvin Haj-Mirzaian Mohammad Hosein Farzaei Mohammad Mojtaba Rohani Freshteh Akramian Rozita Naseri Antoni Sureda Negar Ghanaatian Amir Hossein Abdolghaffari 《Journal of cellular physiology》2019,234(12):21519-21546
Flavonoids comprise a group of natural polyphenols consisting of more than 5,000 subtypes mostly existing in fruits and vegetables. Flavonoids consumption could potentially attenuate the incidence and recurrence risk of colorectal cancers through their antiperoxidative, antioxidant, and anti-inflammatory effects. In addition, these compounds regulate the mitochondrial function, balance the bacterial flora and promote the apoptosis process in cancerous cells. However, some previous data failed to show the effectiveness of flavonoids in reducing the risk of colorectal cancer. In this study, we have reviewed the efficacy of different flavonoids subtypes on the risk of colon cancer and molecular mechanisms involved in this process in both clinical and animal studies. In addition, we tried to elucidate the potential synergy between these compounds and current colorectal cancer treatments. 相似文献
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不同来源中国蜂胶水提物的化学组成及抗氧化性 总被引：1，自引：0，他引：1
本文研究了季节和地理来源对中国蜂胶水提物的化学成分和抗氧化性的影响。分别采集了来自山东、湖北和海南三个省份不同季节的蜂胶,采用改进的提取方法,得到蜂胶水提物(Water extract of propolis,WEP)。分光光度法测定了WEP的主要化学组成,普鲁士兰法和清除DPPH自由基活性法评价了WEP的抗氧化性。研究结果表明,中国WEP含有总酚和总黄酮等化学成分,具有较好的抗氧化性。季节对中国WEP化学成分和抗氧化性有显著影响。山东和湖北WEP化学成分比较接近,但同海南WEP有显著差异,抗氧化活性也显著强于海南WEP。不同产地和不同采收季节的中国WEP存在化学组成及生物活性多样性,在生产实践中需注意这一特性。 相似文献
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杜香不同提取部位的镇痛抗炎作用研究 总被引：3，自引：0，他引：3
采用小鼠醋酸扭体法和角叉菜胶致小鼠足掌肿胀模型筛选杜香三种提取部位镇痛抗炎作用.结果显示,甲醇提取物(10.0、1.0 mg/kg)和水提物(10.0 mg/kg)能显著抑制醋酸引起的小鼠扭体反应和角叉菜胶引起的小鼠足趾水肿.水提物(10.0 mg/kg)在致炎后2～4 h内效果接近吲哚美辛.高效液相色谱结果提示甲醇提取物的镇痛抗炎效果可能通过其所含黄酮类化合物实现. 相似文献
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Ari S. O. Lemos Lara M. Campos Thalita F. Souza Priscila L. Paula João Victor G. Da Silva Elaine S. Coimbra Eugenio D. Hottz Paula R. B. Dib Jair A. K. Aguiar Richard M. Grazul Luciana M. Chedier Rodrigo L. Fabri 《化学与生物多样性》2023,20(1):e202200624
In recent years, natural products with biological activities have been increasingly researched. The elucidation of phytoconstituents is necessary for the development of drugs as a natural alternative for the treatment of various diseases. The work aimed to evaluate in vitro and in silico bioactivities of hexane (CCHE) and methanol (CCME) fractions of ethanolic extract from Centrosema coriaceum Benth (Fabaceae) leaves and elucidate their phytoconstituents. CCHE and CCME showed antifungal activity for Candida glabrata (MIC of 1000 μg/mL) with fungistatic effect and action in cell envelope by sorbitol and ergosterol assays. CCHE and CCME presented promising antioxidant activity against the DPPH radical with IC50 of 13.61±0.50 and 6.31±0.40 μg/mL, respectively, and relative antioxidant activity (RAA%) of 45.77±3.61/ 28.53±2.25 % for CCHE and 82.18±2.25/51.99±3.23 % for CCME when compared to rutin and quercetin, respectively. Moreover, these fractions demonstrated promising results for the inhibition of lipid peroxidation by β-carotene/linoleic acid assay. For anti-inflammatory and cytotoxicity activities, CCHE and CCME significantly inhibited the production of nitric oxide and TNF-α, without toxicity on murine intraperitoneal macrophages, respectively. Esters, alkanes, steroids, tocopherols, and terpenes were identified in CCHE by GC/MS. Flavonoids, phenolic acids, and disaccharides were detected in CCME by UFLC-QTOF-MS and FACE. Furthermore, rutin was purified from CCME. In silico predictions evidenced that compounds present in both fractions have high affinity to the fungal membrane besides antioxidant and anti-inflammatory activities. Based on these observations, CCHE and CCME have a noteworthy potential for the design of novel antifungal and anti-inflammatory agents that should be explored in future studies. 相似文献
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Veridiana V. De Rosso Faustino E. Morán Vieyra Adriana Z. Mercadante 《Free radical research》2013,47(10):885-891
The quenching of singlet molecular oxygen (1O2) by the flavylium cation form of six widespread anthocyanin derivatives: cyanidin 3-glucoside (CG), cyanidin 3-rutinoside (CR), cyanidin 3-galactoside (CGL), malvidin (M), malvidin 3-glucoside (MG) and malvidin 3,5-diglucoside (MDG) was studied in 1% HCl methanol solution by time-resolved phosphorescence detection (TRPD) of 1O2 and photostationary actinometry using perinaphthenone and methylene blue as sensitizers, respectively. The average value of the total (k0) and chemical (kc) quenching rate constants were ～ 4×108 m?1 s?1 and 3×106 m?1 s?1, respectively, indicating the good performance of the studied anthocyanins as catalytic quenchers of 1O2. The quenching efficiency was larger for malvidin than for cyanidin derivatives, probably by the extra electron-donating methoxy group in ring B of the malvidin derivatives; and it was also dependent on the number and type of glycosylated substitution. As observed for other phenolic-like derivatives, the quenching of 1O2 by anthocyanins was mediated by a charge-transfer mechanism, which was modulated by the total number of –OR substituents that increases the electron-donating ability of these compounds. 相似文献