共查询到20条相似文献，搜索用时 46 毫秒
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Multiple mutations of the first gene of a dimeric tRNA gene abolish in vitro tRNA gene transcription 总被引：3，自引：0，他引：3
M Nichols J Bell M S Klekamp P A Weil D S?ll 《The Journal of biological chemistry》1989,264(29):17084-17090
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RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent Km for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNA(Ser) genes is 20 nM; the apparent Vmax is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action. 相似文献
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A single base change in the intron of a serine tRNA affects the rate of RNase P cleavage in vitro and suppressor activity in vivo in Saccharomyces cerevisiae 总被引：15，自引：0，他引：15
I Willis D Frendewey M Nichols A Hottinger-Werlen J Schaack D S?ll 《The Journal of biological chemistry》1986,261(13):5878-5885
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