On the carrying behaviour of basic South American primates

InCallithrix, Saguinus, Aotus, andCallicebus other group members than the mother participate in infant care. Differences among these species are obvious in respect to the time of being off any caregiver, and in respect to the carrying position (Aotus andCallicebus). Alloparental care, a basic behaviour pattern in these species, has evolved from the parking behaviour of the prosimians. The infants are «parked» at other group members.     

Flocculation phenomenon of Candida albicans by lysozyme

Young colonies of Sabouraud's glucose agar room temperature culture ofCandida species from human isolation were suspended in distilled water. The suspension was mixed with a solution of lysozyme and incubated in a 37° C water bath. Within 3–5 hours, various species ofCandida cells showed flocculation to varying degrees which occurred at varying periods of onset. Among sevenCandida species,Candida albicans andCandida stellatoidea showed the strongest flocculation, earliest onset and most solution clarity than did any other species.Candida stellatoidea was indistinguishable fromCandida albicans in its degree of flocculation, and in the clarity of solution.Candida species may be arranged in the following order according to their decreasing positivity in flocculation:

1. Candida albicans
2. Candida stellatoidea
3. Candida tropicalis
4. Candida krusei
5. Candida pseudotropicalis
6. Candida parapsilosis
7. Candida guilliermondii
8. Saccharomyces species may be placed afterCandida guilliermondii.

It seems possible to separate theCandida species into 3 groups by the rate of flocculation, and clarity of solution. Group I.Candida albicans andCandida stellatoidea. Group II.Candida tropicalis, C. krusei andCandida pseudotropicalis. Group III.Candida parapsilosis andCandida guilliermondii. Saccharomyces specimens (S. cerevisiae and others) were placed after group III.     

Molecular evidence to reconcile taxonomic instability in mahseer species (Pisces: Cyprinidae) of India

The mahseers are an important group of fishes endemic to Asia with most species considered threatened. Conservation plans to save declining wild populations are hindered by unstable taxonomy, and detailed systematic review could form a solid platform for future management and conservation. D-loop and cytochrome c oxidase I (COI) mtDNA sequences were examined in nine mahseer species of Tor, Neolissochilus, and Naziritor. Pseudogenes amplified in a portion of the species limited the utility of the D-loop region. ABGD analysis, NJ, ML, and MP methods and genetic distance (TrN?+?I?+?G) using COI data revealed concordant species delimiting patterns. The three genera were monophyletic, separated as distinct clades (TrN?+?I?+?G 0.064 to 0.106), and Naziritor was flagged as a separate genus, distinct from Puntius (TrN?+?I?+?G 0.196). Out of seven nominal species known for Tor cogeners from India, only five were recovered with mtDNA data (TrN?+?I?+?G 0.000 to 0.037) and two species could not be distinguished with the molecular data set employed. Tor mosal, synonymized as Tor putitora, was rediscovered as a distinct species (TrN?+?I?+?G 0.031) based on its type locality. Tor mussulah was confirmed as a separate species (TrN?+?I?+?G 0.019 to 0.026). Two valid species, Tor macrolepis and T. mosal mahanadicus, were not distinct from T. putitora (TrN?+?I?+?G 0.00). The high divergence with mtDNA data failed to validate T. mosal mahanadicus as a subspecies of T. mosal (TrN?+?I?+?G 0.031). Morphological outliers discovered within the distribution range of Tor tor (TrN?+?I?+?G 0.022 to 0.025) shared the same lineage with T. putitora (TrN?+?I?+?G 0.002 to 0.005), indicating a new extended distribution of the Himalayan mahseer T. putitora in the rivers of the Indian central plateau. The findings indicate the need for integrating molecular and morphological tools for taxonomic revision of the Tor and Naziritor genera, so that taxa are precisely defined for accurate in situ and ex situ conservation decisions.     

Centric diatoms from Lake Frolikha (Transbaikal area) and peculiarities of distribution of some taxa in Asia

The species composition of centric diatoms from Lake Frolikha (Transbaikal area) has been studied. The lake is located on the eastern shore of Lake Baikal and connected with it by a river. Twenty-three species of Centrophyceae from 7 genera (Aulacoseira, Cyclostephanos, Cyclotella, Discostella, Handmania, Pliocaenicus, and Stephanodiscus) have been found. The most represented genus is Aulacoseira (11 species). Fifteen species and 4 genera are new for the lake record. All revealed species are known in other lakes of the Baikal region; however Baikal endemics are absent in Lake Frolikha. The flora of centric diatoms in Lake Frolikha can be divided into two groups. The first group includes taxa common in Lake Baikal, and the second group includes taxa not typical for Lake Baikal. The level of differences between flora in Lake Baikal and Lake Frolikha is high (43%) despite the close location and connection to the river. An analysis of distribution of interesting species in Asia is presented.     

Species ofIchneumonidae [Hym.], parasites of some lepidopterous pests of lucerne in Romania

Eleven species ofIchneumonidae were identified as parasites of lepidopterous pests of lucerne. All of the parasitized Lepidoptera are new host records for theseIchneumonidae andDiadegma variegata Szépl. is a species of parasite new to Romania.     

Past hybridization between Quercus macrocarpa and Quercus gambelii

Introgression between two allopatric species,Quercus macrocarpa andQ. gambelii, has been observed in two separate locations: northeastern New Mexico and the Black Hills of western South Dakota and adjacent Wyoming. The probability that this introgression is the result of long-range pollination appears remote. Presumably these two species hybridized during a period of past sympatric association. Further indication of past sympatry in the Black Hills is the presence of a common species of obligate parasite, i.e., wasp(Cynips insulensis) onQ. gambelii in the Rocky Mountains and onQ. macrocarpa of the Black Hills. The hybrid oaks in New Mexico probably reflect a westward migration ofQ. macrocarpa during pluvial periods of the Pleistocene.Quercus gambelii most likely reached the Black Hills during the warmer postglacial hypsithermal era. The hybridization reported here may reflect secondary sympatry, i.e., sympatric occurrence after the species, or their ancestors, became geographically separated.     

Seasonal density,distribution and interactions of predatory and scavenger arthropods in accumulating poultry wastes in coastal and interior Southern California

Seasonal predatory and scavenger arthropod densities were studied at interior and coastal southern California poultry ranches. Though some seasonal population clumping occurred with some species, the distribution of predators and scavengers was fairly uniform within each ranch. Correlation analyses of key predators in theHisteridae, Staphylinidae, Hydrophylidae andDermaptera with the potential hosts,Musca domestica L.,Tinea fuscipunctella Haworth, andFannia spp. suggested that predator activity was seasonally influenced. Possible periodic avoidance of a particular host's habitat was detected as significant negative correlations. The data tend to support the importance of different species of predators in different seasons and the need for natural enemy complexes rather than single species for biological control.     

Supplementary notes on American Labiatae. X

Notes on the taxonomy and distribution of various species of Labiatae of Mexico, and Central and South America, are presented, as part of a continuing series of supplements to the authors’ earlier monographs. Eight new species are described in the generaHyptis, Salvia, Satureja, andScutellaria. Two new combinations are made, one each inHarlanlewisia andSatureja. A key and revision are given for the six species ofLepechinia sect.Parviflorae.     

New genera and species of Chrysobalanaceae from Malesia and Oceania

Two new genera of Chrysobalanaceae are described.Hunga distributed in New Caledonia, the Loyalty Islands and Papua and New Guinea, contains eight species, three new, and five formerly placed in the generaAngelesia, Licania andParinari.Kostermanthus is described to accommodate two species with united filaments formerly placed inParinari andAcioa. Both new genera are quite as distinct as others of the family and have been overlooked by undue emphasis placed on the single character of a bilocular ovary.Licania palawanensis Prance is also described; it is the second species of its genus from Malesia.     

Description ofOceanospirillum kriegii sp. nov. andO. jannaschii sp. nov. and assignment of two species ofAlteromonas to this genus asO. commune comb. nov. andO. vagum comb. nov

Immunological studies of Fe-containing superoxide dismutase (FeSOD) and glutamine synthetase (GS) have established a close relationship betweenOceanospirillum linum (the type species of the genus),O. beijerinckii, Alteromonas communis, A. vaga, and two unnamed species of marine bacteria (groups H-1 and I-1). The four latter species have, consequently, been assigned to the genusOceanospirillum asO. commune comb. nov.,O. vagum comb. nov.,O. kriegii sp. nov. (group H-1; type strain 197, ATCC 27133), andO. jannaschii sp. nov. (group I-1; type strain 207, ATCC 27135). The phenotypic properties of these species are presented together with their distinguishing traits.     

Occurrence of Phytophthora plurivora and other Phytophthora species in oak forests of southern Poland and their association with site conditions and the health status of trees

Phytophthora plurivora and other Phytophthora species are known to be serious pathogens of forest trees. Little is known, however, about the presence of P. plurivora in Polish oak forests and their role in oak decline. The aims of this study were to identify P. plurivora in healthy and declining Quercus robur stands in southern Poland and to demonstrate the relationship between different site factors and the occurrence of P. plurivora. In addition, the virulence of P. plurivora and other Phytophthora species was evaluated through inoculations using 2-year-old oak seedlings. Rhizosphere soil was investigated from 39 oak stands representing different healthy tree statuses. The morphology and DNA sequences of the internal transcribed spacer regions (ITS) of the ribosomal DNA and the mitochondrial cox1 gene were used for identifications. P. plurivora, an oak fine root pathogen, was isolated from rhizosphere soil samples in 6 out of 39 stands. Additionally, Phytophthora cambivora, Phytophthora polonica and Phytophthora rosacearum-like were also obtained from several stands. The results showed a significant association between the presence of P. plurivora and the health status of oak trees. Similar relationships were also observed for all identified Phytophthora species. In addition, there was evidence for a connection between the presence of all identified Phytophthora species and some site conditions. Phytophthora spp. occurred more frequently in declining stands and in silt loam and sandy loam soils with pH?≥?3.66. P. plurivora and P. cambivora were the only species capable of killing whole plants, producing extensive necrosis on seedling stems.     

Freshwater Crustacea (Cladocera,Copepoda) of Iceland: taxonomy,ecology, and biogeography

Of the many types of freshwater bodies across Iceland (e.g., lakes, springs, rivers, and ponds), greater effort has been invested in researching the freshwater Crustacea (Cladocera, Copepoda) of more permanent, larger, and deeper water bodies than on smaller or more ephemeral sites. To address this, we described the fauna of 12 shallow freshwater bodies distributed from the coast to the central highlands of Iceland. We identified 16 species in 11 genera belonging to six families of Cladocera, and 12 species in eight genera belonging to four families of Copepoda. Five of the species we identified (Alona werestschagini, Cyclops vicinus, Daphnia cristata, Diacyclops abyssicola, and Heterocope borealis) represent new distribution records for Iceland, increasing the number of species now known from this region to 34 Cladocera and 38 Copepoda taxa. Additionally, we discuss the taxonomy of some species with doubtful taxonomical status. Six crustacean taxocenes are described, each characterized by the dominance of different taxa, with bottom sediment characteristics best explaining species assemblage structure. We evaluated the relationships between Icelandic Cladocera and Copepoda faunas and those of the adjacent Svalbard, Faroe, and Shetland Islands, Greenland, and the mainland Norway. The taxonomic composition of the Icelandic fauna is dominated by widely distributed Holarctic, and secondarily Palaearctic and Nearctic species, and is more similar to the faunas of the major North Atlantic islands (64.2–71.8 % similarity) than it is to that of continental Norway.     

Two new species of Rhynchospora section Psilocarya (Cyperaceae)

Two new species ofRhynchospora sectionPsilocarya are described and illustrated.Rhynchospora eburnea is known from Amazonian Venezuela and nearby Brazil whileRhynchospora waspamensis is known only from eastern Zelaya, Nicaragua. These species are distinguished from most of the species of sectionPsilocarya by means of a key to the species found in the Americas south to and including the Amazon Basin.     

The Indigo of Commerce in Colonial North America

It can be demonstrated that the Indigo of Commerce in Colonial North America consisted of three species in the genusIndigofera. One of these was a native plant,I. Caroliniana Mill, while the other two were introduced.Indigofera tinctoria L. (French Indigo), an Old World species, andI. Suffruticosa Mill. (Guatemala Indigo), a New World species, were both introduced into South Carolina in the late 17th and early 18th centuries. Their cultivation flourished until the American Revolution. Neither of the introduced species has become naturalized in the Carolinas.     

Leaf flavonoids ofGalium sect.Aparinoides (Rubiaceae)

13 taxa belonging to 4 “species groups” ofGalium L. sect.Aparinoides (Jord.)Gren. produce 15 leaf flavonoids: Apigenin-7-diglucoside, Luteolin-7-monoglucoside and 7-diglucoside, Diosmetin, Diosmetin-7-monoglucoside and 7-diglucoside; Kaempferol-3-rutinoside, Kaempferol-3,7-diglucoside, Quercetin, two Quercetin-3-monoglycosides, Rutin, Quercetin-3-rutinoside-7-glucoside, Quercetin-7-glycoside and an unidentified aglycone. TheG. trifidum, G. obtusum andG. palustre groups (with the exception of theG. tinctorium subspecies andG. elongatum) have similar flavone-flavonole patterns, while theG. antarcticum group produces a specific pattern. Leaf flavonoids of theG. trifidum andG. antarcticum group are inhomogenous, becauseG. tinctorium subsp.tinctorium andG. antarcticum lack flavones. For all taxa (with the exception of those of theG. antarcticum group) intraspecific variation is demonstrated, and 4 populations ofG. trifidum subsp.trifidum, G. tinctorium subsp.tinctorium,G. obtusum subsp.obtusum andG. labradoricum even exhibit intrapopulation variation. The implications of flavonoid data on the systematics and the astonishing intrapopulation and intraspecific variation are discussed.     

The Significance and Mechanism of Propofol on Treatment of Ischemia Reperfusion Induced Lung Injury in Rats

This study is aimed to investigate the efficacy and underlying the mechanism of propofol in treatment of ischemia reperfusion (IR)-induced lung injury in rats, providing a novel insight of therapeutic strategy for IR-induced lung injury. 120 healthy SD rats were selected and randomly divided into sham operation group, IR group, and propofol group (40 rats per group). Bronchoalveolar lavage fluid (BALF) protein content, serum protein content, lung permeability index, lung water content rate, methane dicarboxylic aldehyde (MDA) in lung tissue, superoxide dismutase (SOD), nitric oxide (NO), endothelin (ET-1), toll-like receptor 4 (TLR4), nuclear factor (NF-κB), and tumor necrosis factor-α (TNF-α) were examined and compared among different groups to evaluate the therapeutical effects of propofol on IR-induced lung injury and analyze the mechanism. In sham operation group, neither change in lung tissue nor pulmonary interstitial edema or alveolar wall damage was found under microscope; in IR group, marked pulmonary interstitial edema and alveolar wall damage complicated with inflammatory cell infiltration and hemorrhage were found; in propofol group, alveolar wall widening was observed, however, hemorrhage in alveolar cavity, inflammatory infiltration and tissue damage were less significant than in IR group. At 3 h after reperfusion, BALF protein content, lung permeability index, and lung water content rate were all significantly increased in IR group and propofol group, while the serum protein content was significantly lower than sham operation group (p < 0.05). Moreover, we found that the change of above parameters in propofol group was less significant than in IR group (p < 0.05). No statistically significant difference was found in ET-1 levels in different groups (p > 0.05). In contrast, MDA and NO in IR group and propofol group were significantly increased, while SOD activity was significantly decreased (p < 0.05). Furthermore, the change of above parameters in propofol group was less significant than in IR group (p < 0.05). In addition, mRNAs of TLR4, NF-κB, and TNF-α were significantly increased in IR group and propofol group (p < 0.05) with more significant change in IR group compared with propofol group (p < 0.05). Propofol has protective effects against IR-induced lung injury by improving activity of oxygen radical and restoring NO/ET-1 dynamic balance. Besides, regulation of TLR4, NF-κB, and TNF-α by propofol also play important role in alleviating IR-induced lung injury.     

Parasites ofSpodoptera exigua,S. eridania [Lep.: Noctuidae] andHerpetogramma bipunctalis [Lep.: Pyralidae] collected fromAmaranthus hybridus in field corn

Three species of lepidopterous larvae were collected fromAmaranthus hybridus L. growing in field corn during 1975 and 1976 at Hastings, Florida.Spodoptera exigua (Hubner) was the predominant species in May.Spodoptera eridania (Cramer) was predominant in June andHerpetogramma bipunctalis (F.) in July and August. Nine native species of parasites, representing theBraconidae, Eulophidae, Ichneumonidae andTachinidae, emerged from these larvae.Meteorus autographae Muesebeck emerged from bothS. exigua andS. eridania. TheTachinidae, Winthemia rufopicta (Bigot),Eucelatori rubentis (Coquillett) andLespensia sp., emerged from mixtures ofS. exigua andS. eridania. Apanteles marginiventris (Cresson),Temelucha sp., andChelonus texanus Cresson emerged from bothS. exigua andH. bipunctalis larvae, andEuplectrus platyhypenae Howard andOphion sp. emerged fromS. eridania. All the species of parasites from the lepidopterous larvae that feed onAmaranthus hybridus are also reported as parasites ofS. frugiperda, a serious pest of corn. Therefore these larvae onA. hybridus may be a source of the parasites found attackingS. frugiperda.     

Genetic population structure of Hemigrammocypris rasborella (Cyprinidae) inferred from mtDNA sequences

The genetic population structure of the small cyprinid Hemigrammocypris rasborella, distributed widely in lowlands of western Japan, was examined using partial sequence data of mitochondrial DNA (mtDNA). Molecular phylogenetic analysis revealed that the populations of the western Kyushu region were markedly differentiated from all eastern populations, such that the groups would be comparable to different species; their divergence was inferred to have occurred in the Late Miocene–Pliocene. Also, a largely divergent mtDNA group (with divergence in the early Pleistocene) was found in the Sanyo and northeastern Shikoku regions, forming a secondary contact zone in the western Kinki with the eastern mtDNA group. To date, these aspects of the population structure of H. rasborella appear to be unique among lowland fishes in western Japan. Deeper understanding of the formation processes of freshwater faunas in western Japan will require further comparisons of the phylogeographic patterns and ecological traits of constituent species.