共查询到20条相似文献,搜索用时 15 毫秒
1.
Relative to nematicides with greater fuming capabilities, 1,2-dibromo-3-chloropropane (DBCP) moved nonuniformly through soil. DBCP concentrations in soil were reduced by low soil temperature and the presence of lime or roots within the soil profile, Applications by either water or chisel injection provided DBCP movement to 120 cm and below. Concentrations were least persistent in the upper 15 cm of the field surface and in one situation where application was not followed by irrigation. Values for Henry''s Constant are reported for DBCP at a range of solution temperatures. Certain advantages and disadvantages of soil atmosphere sampling of DBCP are discussed. 相似文献
2.
Gas-liquid chromatography was used to detect movement of the nematicide, 1,2-dibromo-3-chloropropane (DBCP), in soil columns containing top- and subsoil of Astatula fine sand. Topsoil contained 1.4-1.6% organic matter and subsoil 0.20-0.25%. DBCP was applied at various rates as aqueous drenches. Depth of penetration was controlled by organic matter in topsoil and varied with the amount of water applied. Maximum DBCP penetration after 14 days was 28 cm; maximum water infiltration, 115 cm. Maximum depth of penetration was obtained with a water emulsion of 30 μg/ml of DBCP applied in 15 cm of water. DBCP applied in 5 cm of water to soils containing 2.0% and 0.125% organic matter penetrated 6 cm and 60 cm, respectively. 相似文献
3.
Yields of ''McNair 800'' soybeans, Glycine max (L.) Merr., were significantly increased with ethylene dibromide + chloropicrin, DBCP, phenamiphos, and aldicarb applied at-planting and with phenamiphos, aldicarb, and DBCP applied postplant to soil infested with Meloidogyne incognita (Kofoid and White) Chitwood. Yields of ''GaSoy 17'' were significantly increased with ethylene dibromide + chloropicrin, DBCP, phenamiphos, and aldicarb applied, preplant and with DBCP, carbofuran, phenamiphos, aldicarb, and DBCP applied postplant to soil infested with Hoplolaimus columbus Sher. In several instances, preplant or at-planting treatments plus postplant treatments with the same or different chemicals were more effective than either treatment alone. Generally, the fumigants were more effective than the nonfumigants when they were applied at-planting to M. incognita-infested soil and preplant to H. columbus-infested soil. Phenamiphos, aldicarb, and DBCP were about equally effective when they were applied postplant in M. incognita-infested soil, but DBCP was more effective than carbofuran. Carbofuran, phenamiphos, aldicarb, and DBCP were about equally effective when applied postplant to H. columbus-infested soil. 相似文献
4.
Movement and persistence of 1,2-dibrotno-3-chloropropane (DBCP) in a Coastal Plain soil containing a sandy plow-pan were enhanced in each of 2 years by subsoiling, increased depth of application, and increased rate of application. DBCP was extracted from the soil with hexane and analyzed by gas chromatography. Subsoiling at a 35-cm depth gave the greatest increase in lateral movement and downward penetration of DBCP in 1975 (a wet year), but bad less effect in 1976 (a dry year). An increased application rate (10 kg/ha vs. 13.5) improved coverage moderately in 1975 by increasing lateral movement, but had little effect in 1976. Increased application depth (18 vs. 35 cm) improved coverage in both years though more in 1976. Deep placement extended DBCP retention time. Rainfall in 1975 probably decreased the number and size of air-filled pores, slowing loss of DBCP to the atmosphere. Because of reduced porosity, the plow-pan was impervious to the passage of DBCP unless disrupted by subsoiling. 相似文献
5.
Genetic toxicology of 1,2-dibromo-3-chloropropane (DBCP) 总被引:1,自引:0,他引:1
1,2-Dibromo-3-chloropropane (DBCP) is a nematocide, which has been used extensively as a soil fumigant in agriculture. Since sterility was found among male workers involved in the manufacture of DBCP, great concern has been focused on the genetic hazards of DBCP. DBCP gave positive results in many tests such as microbial, in vitro cytogenetics, and Drosophila studies. In mammalian test systems, DBCP caused chromosomal aberrations in the bone marrow cells and dominant-lethal mutations in germ cells in rats. In mice, there were no signs of DBCP-induced heritable mutation in germ cells, although point mutations were detected in somatic cells. The occurrence of Y-chromosomal non-disjunction was indicated in DBCP-exposed male workers by an increased number of sperm containing 2 Y-chromosomes. 相似文献
6.
广西北部罗汉果根结线虫病研究 总被引:7,自引:0,他引:7
罗汉果根结线虫是罗汉果的一个重要病害。在室内盆栽接种条件下,该线虫年发生6代。本文叙述了线虫的生物学及其寄主植物。防治试验结果表明。穴施灭克磷等是有效的。土壤翻晒也能消灭大部分土中线虫,病薯用热处理也有很好的效果。 相似文献
7.
S Palmero M Leone M Prati M Costa M Messeni Leone E Fugassa L De Cecco 《Hormones et métabolisme》1990,22(12):622-626
The effect of L-acetylcarnitine (LAC) on some parameters of male reproductive function was studied on rats made oligoasthenospermic with dibromochloropropane (DBCP). DBCP depresses sperm count and motility. After one injection of the drug, LAC induces a recovery of both sperm count and motility but after two injections it is ineffective. This effect is also shown visually by microscopic examination of seminiferous tubules. Among the enzymatic activities evaluated as biochemical markers of testicular function both lactate dehydrogenase and NADPH-cytochrome P 450-reductase increased significantly (P less than 0.05) after treatment with LAC in normal rats. LAC also stimulates testosterone production. It is suggested that LAC may affect testicular function. 相似文献
8.
Four methods of placement of DBCP (l,2-dibromo-3-chloropropane) and a single method of application of ethoprop (0-ethyl S,S-dipropyl phosphorodithioate) wexe compared in each of two areas for control of nematodes on soybeans. One area was a Marlboro sand infested with Hoplolaimus columbus. The other area was a Fuquay loamy sand infested with Meloidogne incognita. Soybean yields were increased and numbers of H. columbus in the row 0-20 cm deep were decreased similarly by all methods of DBCP application in Marlboro soil. All DBCP treatments increased the average soybean yields and decreased numbers of M. incognita larvae in the row 0-20 cm deep in the Fuquay soil. Average root-knot indices were reduced by all DBCP treatments except with placement 40 cm deep beneath the row. Similarly, placement of all or part of the DBCP 20 cm deep and 13 cm to either side of the row resulted in greater average yields than placement of the DBCP 40 cm deep. Apparently, control of M. incognita is more critical 0-20 cm deep than 20-40 cm deep for increasing soybean yields. DBCP did not control H. columbus as effectively as it did M. incognita. Control of H. columbus and M. incognita was not obtained at 0-20-cm and 20-40-cm depths 30 cm and 45 cm from the row regardless of the method used to apply DBCP. H. columbus and M. incognita were controlled more effectively and soybean yields were higher with DBCP at 13.6 kg a.i./ha than with ethoprop at 4.5 kg a.i./ha. 相似文献
9.
The nematocide and soil fumigant 1,2-dibromo-3-chloropropane (DBCP) is a carcinogen and a mutagen and displays target-organ toxicity to the testes and the kidney. It has been proposed that both cytochrome P-450 mediated activation and glutathione (GSH) conjugation pathways are operative in DNA damage and organotropy induced by DBCP. To determine the chemical mechanisms involved in the bioactivation of DBCP and to assess a role for an episulfonium ion intermediate, the mechanism of formation of GSH conjugate metabolites of DBCP was investigated. Five biliary GSH conjugates of DBCP were isolated from rats and identified by fast atom bombardment tandem mass spectrometry: S-(2,3-dihydroxy-propyl)glutathione (I), S-(2-hydroxypropyl)glutathione (IIA), S-(3-chloro-2-hydroxypropyl)glutathione (III), 1,3-di(S-glutathionyl)propan-2-ol (IV), and 1-(glycyl-S-cysteinyl)-3- (S-glutathionyl)propan-2-ol (V). The mechanisms of conjugate formation were addressed by assessing deuterium retention in conjugates derived from [1,1,2,3,3-2H5] DBCP (D5-DBCP). GSH conjugates I, III, IV, and V displayed quantitative retention of deuterium, an observation consistent with the formation of an episulfonium ion intermediate. GSH conjugate IIA, however, retained three atoms of deuterium, thus invoking a P-450 mechanism in its genesis. The involvement of glutathione transferase (GST) and sequential episulfonium ion intermediates in the formation of metabolites I, III, and IV was demonstrated in vitro. Upon incubation of DBCP with GST, metabolites I, III, and IV were identified by tandem mass spectrometry and were found to arise with quantitative retention of deuterium when D5-DBCP was employed as a substrate. An additional GSH conjugate, 1,2,3-tri(S-glutathionyl)propane (VI), was observed as the major metabolite in incubations of GST with DBCP. When the incubations of DBCP with GST were performed in H2(18)O, metabolite I incorporated two atoms of 18O, and metabolites III and IV incorporated one atom of 18O. The ability of GST to catalyze the formation of the four GSH conjugates observed in vivo, with quantitative retention of deuterium and incorporation of 18O from H2(18)O, may be rationalized by a mechanism invoking the initial formation of S-(2-bromo-3-chloropropyl)glutathione. Rearrangement of this unstable conjugate via several reactive episulfonium ions, with either hydrolysis by water or alkylation of GSH at various stages, would account for the pattern of metabolites and their status of isotopic enrichment observed under various incubation conditions.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
10.
Laboratory experiments were conducted by applying 1,2-dibromo-3-chloropropane (DBCP) to sealed vials of soil infested with Meloidogyne javanica. A minimum initial concentration of 0.25 μg of DBCP/g of oven-dry soil killed all nematodes within 35 days. A concentration of 1.0 μg/g killed all nematodes within 28 days. The rate of degradation of this chemical was determined by treatment of steamed and nonsteamed dry soil in open and sealed vials. Extraction of tile chemical, followed by quantification by gas chromatography, showed approximately 100% of the amount applied recovered after 14 days in sealed vials without soil. With soil present, approximately 10% of the amount of chemical applied was recovered. 相似文献
11.
Samir A. Kouzi Erik J. Sderlund Erik Dybing John H. N. Meerman Sidney D. Nelson 《Chirality》1995,7(5):359-364
The haloalkane 1,2-dibromo-3-chloropropane (DBCP), an environmental pollutant that was widely used as a soil fumigant, is a carcinogen and a mutagen and displays target-organ toxicity to the testes and the kidneys. Because little is known about effects of stereochemistry on the metabolism and toxicity of halogenated alkyl compounds and because DBCP, which has a chiral center at C-2, may show enantioselectivity in its metabolism and/or toxicities, the optically pure enantiomers of DBCP were tested in vivo in rats for organ toxicity as well as for bacterial mutagenicity. Organ toxicity studies showed that (S)-DBCP was slightly more renal toxic than (R)-DBCP but was not significantly more toxic than the racemate, and that no significant differences were observed in the extents of testicular necrosis and atrophy caused by either enantiomer or the racemate. In contrast, (R)-DBCP was more mutagenic than either (S)-DBCP or the racemate to Salmonella typhimurium (S. typhimurium) strains TA 100 and TA104. However, there was little or no enantioselectivity in glutathione S-transferase (GST)-catalyzed conjugation reactions of glutathione with DBCP based on the lack of selectivity in the rates of disappearance of the enantiomers of DBCP in the presence of glutathione (GSH) and GSTs as monitored by chiral gas chromatography (GC). © 1995 Wiley-Liss, Inc. 相似文献
12.
Twelve soil drenches over a period of 30 days with DBCP concentrations of 40 μg/ml did not completely prevent infection of tomato plants by root-knot nematode juveniles. Repeated DBCP drenches of 40 μg/ml halted gall development during the drenches, but 10 days after drenching was discontinued galls were apparent. DBCP drenches at 200 μg/ml prevented tomato root development, and 40 μg/ml slowed it. Ten μg/ml increased the height of root-knot-infected plants, but not their top weights. Treated plants were lanky. Protective drenches of 2.5 to 40 μg/ml of DBCP decreased nematode populations and increased fruitfulness. DBCP as a therapeutant reduced the incidence of galling on new roots and halted increases in gall size on previously infected roots but did not improve fruitfulness or plant size significantly. 相似文献
13.
Dr. Jørn A. Holme Erik J. Søderlund Gunnar Brundorg Marit Låg Sidney D. Nelson Erik Dybing 《Cell biology and toxicology》1991,7(4):413-432
1,2-Dibromo-3-chloropropane (DBCP) and a number of halogenated propane analogs induced DNA damage in rat hepatocytes in vitro measured by an automated alkaline elution method. Short-term (2 hrs) cytotoxic effects of DBCP were not observed until the DBCP concentration exceeded 1 mM. The short-term cytotoxicity of all the DBCP analogs occurred in the same concentration range. Significant membrane damage, measured as cell detachment, was observed after extended exposure to lower concentrations of DBCP (100 M) for 20 hrs. The relative, delayed cytotoxic effect of DBCP and analogs correlated with their ability to cause DNA damage. In general, the halogenated propanes with more bromines relative to chlorines were the more potent compounds. Propane analogs lacking the third halogen had little cytotoxic activity. The addition of the proposed specific poly(ADP-ribosyl)transferase inhibitor 3-aminobenzamide (3-ABA) protected against DBCP-induced cytotoxic effects and NAD+ depletion. However, 3-ABA also reduced DBCP-induced DNA damage, DBCP metabolic loss, and the formation of water soluble and covalently bound DBCP metabolites. Thus, 3-ABA may block DBCP-induced cell death by decreasing the formation of reactive DBCP-metabolites.Abbreviations 3-ABA
3-aminobenzamide
- 3-AB acid
3-aminobenzoic acid
- Asc
ascorbate
- BSA
bovine serum albumin
- DBCP, 1,2-diB-3-CP
1,2-dibromo-3-chloropropane
- DMSO
dimethylsulfoxide
- DPPD
N,N-diphenyl-p-phenylenediamine
- GSH
glutathione
- Hoechst 33258
[2(2-(4-hydroxy-phenyl)-6-benzimidazole-6-(1-methyl-4-piperazyl)-benzimidazole trihydrochloride)]
- 1,2,3-triBP
1,2,3-tribromopropane
- 1,3-diB-2-CP
1,3-dibromo-2-chloropropane
- 1,3-diC-2-BP
1,3-dichloro-2-bromopropane
- 1,2,3-triCP
1,2,3-trichloropropane
- 1,2-diBP
1,2-dibromopropane 相似文献
14.
An automated alkaline elution system: DNA damage induced by 1,2-dibromo-3-chloropropane in vivo and in vitro 总被引:2,自引:0,他引:2
G Brunborg J A Holme E J S?derlund J G Omichinski E Dybing 《Analytical biochemistry》1988,174(2):522-536
An automated alkaline elution system for the detection of DNA damage has been developed. After manual application of samples, which is completed within 5 min, the subsequent supply of liquids, changes in flow rates, and temperature are controlled automatically. The system operates 16 filters and may easily be expanded. The sensitivity of the fluorometric DNA determinations with the Hoechst 33258 dye is increased by using an elution buffer (20 mM Na2EDTA, pH 12.50) with low background fluorescence. DNA is determined using an automated setup similar to the one recently presented by Sterzel et al. (1985, Anal. Biochem. 147, 462-467). The most significant modification is the use of a neutralization buffer which allows variations in the pH of eluted fractions. This change increases the sensitivity of the DNA measurements. The automated alkaline elution system was evaluated using the nematocide 1,2-dibromo-3-chloropropane (DBCP) in a study of its genotoxic effects in the testes and the kidneys. Significant DNA damage was induced in testicular cells by 2.5 microM DBCP (1 h) in vitro and 85 mumol/kg DBCP ip (3 h) in vivo. The damage appeared after short treatment times (10 min in vivo). Variations in the observed DBCP response in vivo were largely due to interanimal variations. The automated alkaline elution system proved to be a sensitive assay also for the detection of DNA damage in kidney nuclei prepared from rats exposed to DBCP. Provided that kidney nuclei from untreated rats, mice, or hamster were kept ice-cold until lysing, 85-100% of their DNA was retained after 16 h of elution, indicating highly intact DNA. Under the same conditions, guinea pig DNA was rapidly degraded unless the nuclei were prepared in a buffer with a higher concentration of Na2EDTA (20 mM). 相似文献
15.
The pesticide 1,2-dibromo-3-chloropropane (DBCP) is a known male reproductive toxin. Previous studies employed production-grade DBCP containing allyl chloride and epichlorohydrin, both capable of producing effects similar to DBCP. The purpose of this study was to determine if purified DBCP caused the same effects as DBCP containing allyl chloride. Rats were injected for 6 mo with varying doses of pure or production-grade DBCP. Very few differences were observed in the parameters measured between pure and production-grade DBCP-treated animals at any one dose level. Treatment with 25 mg/kg DBCP, pure or production grade, reduced body weight as well as the weight of the testes, prostate glands and seminal vesicles, and elevated serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This dose of pure DBCP reduced serum testosterone. Treatment with 5 mg/kg of either grade DBCP caused decreases only in body and testis weights. Animals treated with 1 mg/kg did not show any major differences from controls. These results indicate that DBCP, and probably not a contaminant, is responsible for the effects observed on male reproduction. Furthermore, DBCP appears to affect either androgen action or production since multiple androgenic indices are affected by DBCP administration. 相似文献
16.
M L?g E J S?derlund J G Omichinski S D Nelson E Dybing 《Chemico-biological interactions》1989,69(1):33-44
In vitro bromide release and in vivo glutathione (GSH) depletion in rat liver, kidney and testis by 1,2-dibromo-3-chloropropane (DBCP) and selectively methylated and deuterated DBCP analogs were studied. With liver microsomes from phenobarbital-pretreated rats the bromide release from the C1-C3-D4- and the perdeuterated DBCP analogs were 54% and 26% of that of DBCP, respectively. Inhibitors of P-450 reduced the bromide release to 10-20% of that without additions. This correlated with the effects of deuterium substitution and additions of P-450 inhibitors on DBCP-induced bacterial mutagenicity as reported elsewhere by this laboratory. To study the importance of GSH-dependent metabolism in DBCP toxicity, bromide release was assayed in cytosolic preparations using methylated analogs of DBCP. With the C1-methyl-derivative, bromide release was markedly reduced compared to that with DBCP in cytosols from liver, kidney and testis. A similar reduction in in vivo nephrotoxicity and testicular damage has recently been reported. The obtained correlation between in vitro GSH-dependent metabolism of methylated DBCP analogs and their in vivo organ damaging potential, points to an involvement of GSH-dependent metabolism in DBCP-induced in vivo toxicity. Both DBCP and the methylated analogs (360 mumol/kg i.p.) depleted the GSH levels in liver after 1 and 3 h and in kidney after 1 h, whereas in the testis no significant depletion of GSH was obtained. As kidney and testis are reported to be the primary target organs for DBCP, there was an apparent lack of correlation between tissue depletion of GSH and organ toxicity. 相似文献
17.
Soil application of DBCP (l,2-dibromo-3-chloropropane) and foliar applications of oxamyl (methyl N'',N''-dimethyl-N-[(methylcarbamoyl)oxy]-l-thiooxamimidate) were compared for control of Tylenchulus semipenetrans in a grapefruit (Citrus paradisi) orchard, DBCP reduced nematode populations and increased fruit growth rate, fruit size at harvest, and yield compared to the untreated controls in the 2 years following treatments. Foliar applications of oxamyl reduced nematode populations and increased fruit growth rate slightly the first year, but not in the second. Foliar applications of oxamyl did not improve control attained by DBCP alone. Soil application of aldicarb [2-methyl-2-(methylthio)propionaldehyde-O-(methylcarbamoyl)oxime] or DBCP to an orange (C. sinensis) orchard reduced T. semipenetrans populations in the 3 years tested and increased yield in 1 of 3 years. Aldicarb treatment reduced fruit damage caused by the citrus rust mite, Phyllocoptruta oleivora. Aldicarb, applied at 5.7 or 11.4 kg/ha, by disk incorporation or chisel injection, was equally effective in controlling nematodes, improving yields, fruit size, and external quality. In a grapefruit orchard, chisel-applied aldicarb reduced nematode populations and rust mite damage and increased yields in both years and increased fruit size in one year. The 11.4-kg/ha rate was slightly more effective than the 5.7-kg/ha rate. Aldicarb appears to be an adequate substitute for DBCP for nematode control in Texas citrus orchards and well-suited to an overall pest management system for Texas citrus. 相似文献
18.
1,2-Dibromo-3-chloro-2-methylpropane (DBCMP) and 1,2,3- tribromo-2-methylpropane (TBMP) are contaminants formed during the manufacture of bromobutyl rubber. These chemicals are structurally similar to 1,2-dibromo-3-chloropropane (DBCP), a known genotoxin and rodent carcinogen. The present study compared the genotoxic properties of DBCMP and TBMP to those of DBCP. In the Salmonella assay, DBCP was positive in strains TA98, TA-100 and TA-1535 in the presence of exogenous activation; DBCP was weakly active in TA-1535 in the absence of activation. Neither DBCMP nor TBMP produced reproducible evidence of mutagenic activity in the Salmonella assay despite the use of several different variations of this test. In the mouse lymphoma gene mutation assay DBCP and TBMP were positive in the presence and absence of activation, while DBCMP was positive only in the absence of activation. All three test compounds were active in the Syrian hamster embryo morphologic transformation assay. The results indicated that both DBCMP and TBMP exhibited some genotoxic activity as did DBCP. The presence of the methyl group on the 2-carbon position essentially eliminated the mutagenicity of DBCMP and TBMP in the Salmonella assay.abbreviations CHO
Chinese hamster ovary cells
- DBCMP
1,2-dibromo-3-chloro-2-methylpropane
- DBCP
1,2-dibromo-3-chloropropane
- DMEM
Dulbecco's Eagle's minimal E medium
- MNNG
N-methyl-N'-nitro-N-nitrosoguanidine
- S-9
microsomal fraction from rodent liver
- TBMP
1,2,3-tribromo-2-methylpropane
- TBP
1,2,3-tribromopropane
- TFT
trifluorothymidine 相似文献
19.
《Bioscience, biotechnology, and biochemistry》2013,77(5):1090-1094
The production of digested bacterial cell powder (DBCP) as a source of reduced-form folates for pigs was studied. Trimethoprim-resistant mutants of Brevibacterium lactofermentum ATCC 13869 accumulated a significantly higher amount of the reduced form of folate in the cells than the wild-type strain. DBCPs were prepared from the resistant mutant strain and the wild-type strain. The utilization of the reduced-form of folate in DBCP was evaluated by measuring the plasma folate level after orally administering DBCP to Göttingen minipigs. The folates in both DBCPs proved to have equally high bioavailability in the pigs. 相似文献
20.
Y Toride M Tominaga H Takeuchi S Miyashiro Y Mizuno E Kokue 《Bioscience, biotechnology, and biochemistry》2001,65(5):1090-1094
The production of digested bacterial cell powder (DBCP) as a source of reduced-form folates for pigs was studied. Trimethoprim-resistant mutants of Brevibacterium lactofermentum ATCC 13869 accumulated a significantly higher amount of the reduced form of folate in the cells than the wild-type strain. DBCPs were prepared from the resistant mutant strain and the wild-type strain. The utilization of the reduced-form of folate in DBCP was evaluated by measuring the plasma folate level after orally administering DBCP to G?ttingen minipigs. The folates in both DBCPs proved to have equally high bioavailability in the pigs. 相似文献