Environment-dependent survival of Drosophila melanogaster: a quantitative genetic analysis

Summary Survival under starvation conditions was investigated in relationship to survival when food was present because these traits could be linked by evolutionary history. Recombinant inbred lines derived from natural populations of Drosophila melanogaster were used to test genetic correlations and architecture of these survival traits. Sexes were genetically correlated within traits and there was significant correlation between survival traits. A number of quantitative trait loci (QTLs) were present for starvation survival and/or survival on food. In general, the QTL effects were consistent for sexes and environments. QTL effects were found on each major chromosome, but the major effects were largely localized on the second chromosome. Importantly, the 'four-allele' progenitor of the recombinant inbred lines used in the present study allowed the sign and magnitude of effects to be assigned to linkage groups. One such linkage group on the second chromosome conferred starvation resistance and longevity, supporting the hypothesis of an association between starvation resistance and lifespan.     

Environmental sensitivity and heterosis for egg laying in Drosophila melanogaster

Summary Genotype × temperature interactions for egg laying were studied in Drosophila melanogaster using two sets of half diallel crosses: one between inbred lines of the same geographic origin, and the other between established laboratory, newly derived inbred lines from different geographic origins. The sensitivity of most genotypes to changes in temperature was adequately described as a linear regression of mean in temperature. The regression coefficients (linear sensitivities) were heterogeneous between genotypes. Hybrids were more affected by temperature variation than were inbreds. All the heterogeneity of linear sensitivities was accounted for by a linear function of the genotypic means, which strongly suggests that a scale effect is responsible for the differences in sensitivity to temperature. In contrast, no general relationship was found between standard error deviation (sensitivity to small environmental changes) and mean performance between genotypes, although hybrids tended to be less variable than inbreds. This shows that the sensitivity to environmental variation depends not only on the genotype, but also on the nature of the environmental variation. The variability within temperatures may be affected by the general homeostasis of individual genotypes, while the variability between temperatures could be the result of genes directly affecting the trait and their multiplicative interaction with the environment.     

Prediction of heterosis in crosses between inbred lines of Drosophila melanogaster

Summary The aim of the experiment was to determine if the estimated genetic distance between two populations could be used to predict the amount of heterosis that would occur when they were crossed. Eight lines of known relatedness to each other were produced by eight generations of sib mating and sub-lining. This produced lines that varied in coefficient of coancestry from zero to 0.78. Fourteen reciprocal crosses of these lines were used to measure heterosis for larval viability and adult fecundity. Gene frequencies at six polymorphic enzyme loci were used to estimate the genetic distances between lines, which were then compared with the known degrees of coancestry. The estimated genetic differences were poorly correlated with the known coancestry coefficients (r=0.4), possibly due to the small number of loci typed. Also genetic distances were only about 1/3 of what was expected. Selection acting on blocks of genes linked to the enzyme loci probably prevented the expected increase in homozygosity. Coancestry coefficient was correlated with heterosis (r=0.44–0.71). This level of correlation implied differences in heterosis among parent lines with the same level of coancestry. This variability is expected if a small number of loci explain most of the heterosis. The average level of heterosis was less than expected after eight generations of sib mating. This is most likely due to selection opposing the increase in homozygosity caused by inbreeding. The combination of these two imperfect correlations resulted in no significant correlation between genetic distance estimated from markers and heterosis.     

Characteristics of recombination of heterosis hybrids of Drosophila melanogaster in high temperature conditions

Spontaneous and high temperature-induced crossing over in regions b-cn and cn-vg of chromosome 2 was studied in Drosophila stocks and hybrids differing in fertility level and heat resistance. No essential distinction in recombination frequency was revealed in variants studied under the temperature 21-22 degrees C. The effect of temperature on 1- and 10-days imago during 4 or 7 h under 35 degrees C leads to increase in exchange frequency in the region b-cn. The hybrids show weaker reaction to this influence.     

Meiosis in Drosophila melanogaster

Individual bivalents or chromosomes have been identified in Drosophila melanogaster spermatocytes at metaphase I, anaphase I, metaphase II and anaphase II in electron micrographs of serial sections. Identification was based on a combination of chromosome volume analysis, bivalent topology, and kinetochore position. — Kinetochore microtubule numbers have been obtained for the identified chromosomes at all four meiotic stages. Average numbers in D. melanogaster are relatively low compared to reported numbers of other higher eukaryotes. There are no differences in kinetochore microtubule numbers within a stage despite a large (approximately tenfold) difference in chromosome volume between the largest and the smallest chromosome. A comparison between the two meiotic metaphases (metaphase I and metaphase II) reveals that metaphase I kinetochores possess twice as many microtubules as metaphase II kinetochores. — Other microtubules in addition to those that end on or penetrate the kinetochore are found in the vicinity of the kinetochore. These microtubules penetrate the chromosome rather than the kinetochore proper and are more numerous at metaphase I than at the other division stages.     

Meiosis in Drosophila melanogaster

Chromosome pairing during meiosis I in D. melanogaster males was investigated ultrastructurally by examining complete bivalents in electron micrographs of serial thin sections. The XY bivalent is characterized by the presence of unique material located between the two half-bivalents at the site of synapsis. The material has a fibrillar appearance and is less electron dense than the surrounding chromatin. YY bivalents in XYY males and XY bivalents containing the X chromosome, In(1)sc ^4Lsc^8R, where the pairing sites of the X chromosome are inverted and partially deleted also possess this material. The material is not associated with autosomal bivalents and may represent a morphological manifestation of the hypothetical cohesive elements (collochores) which are thought to function in conjunction of the X and Y chromosomes (Cooper, 1964).     

Neurophysiological Genetics in Drosophila melanogaster

Neurophysiological genetics is the study of the mechanisms bywhich genes control nervous function and behavior. The transductionof genetic information into neural information is studied atthe level of the neuron through genetic and physiological techniques. The neurons responsible for the leg-shaking action specificto a single-gene mutant of Drosophila melanogaster, Hk¹, havebeen located in three pairs of small regions in the thoracicganglion. The activity pattern of these neurons is coded bythe mutant Hk¹ gene. The center for the specifically patternedleg-shaking action is composed of several motor neurons whoseactivity is governed by the pacemaking activity of at leastone interneuron. As it is most likely that the mutant gene isexpressed autonomously in this interneuron, there is a possibilityof investigating ways in which genes may influence the propertiesof neurons. The activity of the mutant neuron was monitoredintracellularly, and the pattern formation mechanism was studied.The amplitude, duration, and periodicity of the pacemaker potentialand the spike initiation site determine the activity patternresulting in the specific leg-shaking action.     

Fat-body remodeling in Drosophila melanogaster

The remodeling of the larval fat body is observed in many insects during metamorphosis, but little is known about the physiological importance or the regulation of this process. In Drosophila melanogaster, fat-body remodeling involves the dissociation of the fat body into individual fat cells, which persist throughout pupal development but are later removed by cell death in the young adult. Inhibition of fat-body dissociation is associated with pharate adult lethality and thus is likely to be an essential developmental event. As a start toward understanding the role of fat-body remodeling in the life history of insects, we carried out a detailed study of fat-body disassociation in D. melanogaster using fluorescent microscopy, and tested whether this process is mediated by hemocytes as proposed for fat-body remodeling in Sarcophaga peregrina. We identified and correlated stereotypic events in fat-body dissociation with developmental changes during metamorphosis, and have demonstrated by cell ablation studies that fat-body remodeling in D. melanogaster is a hemocyte independent process.     

Tandem duplications in Drosophila melanogaster

In the tandem duplication Dp(1;1)Gr approximately one quarter of the euchromatic part of the X-chromosome is duplicated. Dp(1;1)Gr itself has no phenotypic effect, but it can be made visible by combining different alleles within the tandem duplication and the homologous X-chromosome. In heterozygous females crossing-over between the two X-chromosomes is strongly reduced while at the same time crossing-over in the distal regions of the autosomes is increased.