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1.
Rongtao Fu Cheng Chen Jian Wang Yongxia Ke Daihua Lu 《Journal of Phytopathology》2019,167(7-8):440-444
Severe brown leaf spot disease was observed on Paris polyphylla var. chinensis in Sichuan Province, China, in 2017 and 2018. The initial symptoms were many light‐brown small spots with necrotic centres, round or irregular in shape, becoming dark brown, gradually enlarging and eventually coalescing, causing extensive leaf senescence. A fungus was isolated from diseased leaves showing typical symptoms of brown leaf spot. The isolates were cultured on potato sucrose agar, and their morphological characteristics of the causal pathogen were observed under a light microscope. Pathogenicity tests revealed that this fungus was the causal pathogen of the disease. Molecular analyses of the sequences of the ribosomal DNA internal transcribed spacer (ITS) region, translation elongation factor 1‐alpha (TEF) and the RNA polymerase II second largest subunit (RBP2) gene were conducted to confirm the identity of the pathogen. The multi‐gene phylogeny indicated that the causal agent was Alternaria tenuissima. To our knowledge, this is the first report of A. tenuissima causing brown leaf sports on P. polyphylla var. chinensis in China. 相似文献
2.
Vijya Rabari Purvi Rakhashiya Pooja Patel Vrinda Thaker 《Journal of Phytopathology》2016,164(4):286-289
A leaf spot disease was observed on Mangifera indica L. located in the region of Rajkot, Gujarat, India. The symptoms included the appearance of small, circular to oval dark brown necrotic sunken spots on the leaves. The fungus formed grey to dark grey on potato dextrose agar and conidia were single‐celled, ovoid or oblong. On the basis of mycological characteristics, pathogenicity test and molecular identification using 28S rDNA sequence and internal transcribed spacer (ITS), the fungus was identified as Botryosphaeria dothidea. To the best of our knowledge, this is the first report of leaf spot on M. indica tree caused by Botryoshaeria dothidea in Gujarat, India. 相似文献
3.
Pavel Dodonov Andreza L. Braga Karen A. Harper Dalva M. Silva Matos 《Austral ecology》2017,42(2):187-197
Edge influence, characterized by differences in ecosystem characteristics between the edge and the interior of remnants in fragmented landscapes, affects a variety of organisms and ecosystem processes. An important feature that may be affected by edges is the amount of plant litter, which provides important habitat for a large variety of organisms and influences ecological processes such as fire dynamics. We studied edge influence on plant litter and fine woody debris in the cerrado of São Paulo state, south‐eastern Brazil. We collected, sorted, dried and weighed plant litter along 180 m‐long transects perpendicular to three savanna and eleven forest edges adjacent to different anthropogenic land uses, with four to five transect per edge. There tended to be less biomass of the finer portions of fine woody debris at both savanna and forest edges. Graminoid litter at savanna edges was greater than in the corresponding interior areas, whereas other litter portions were either unaffected by edges or did not show consistent patterns in either savanna or forest. Edge influence was usually restricted to the first 20 m from the edge, was not influenced by edge characteristics and exhibited no clear differences between savanna and forest areas. Several mechanisms may have led to the variable patterns observed including variation in the plant community, plant architecture, and invasive species. The edge‐related variation in plant litter may putatively lead to, for example, increased fire frequency and intensity at the savanna edges and altered trophic dynamics at forest edges; the mechanisms and consequences of this edge influence should be addressed in future studies. 相似文献
4.
In 2012, dark brown spots were observed on leaves of Ledebouriella seseloides (Fang Feng) in several research plots located at the Goseong Agricultural Research Extension services in Gyeongam Province, Republic of Korea. A fungus was isolated from the infected plants which produced pink‐coloured spores in mucilage on PDA and conidial morphology suggested that the causal agent was Colletotrichum gloeosporioides. Internal transcribed spacer sequences of the pathogen showed 99% identity to those of C. gloeosporioides. Pathogenicity of the isolate was proved by Koch's postulates. This is the first report of anthracnose in L. seseloides caused by C. gloeosporioides. 相似文献
5.
Characterization of Colletotrichum orchidophilum,the agent of black spot disease of vanilla
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Carine Charron Jacqueline Hubert Janice Minatchy Véronique Wilson Fabiola Chrysot Scholastie Gerville Renaud Ioos Céline Jeandel Michel Grisoni 《Journal of Phytopathology》2018,166(7-8):525-531
Black spot symptoms were reported on vanilla plants in Reunion Island in 2011. They have repeatedly reduced annual pod production by 10% to 30%. The disease is characterized by dark spots that appear in slight depressions on flowers, pods, leaves and stems. The spots then develop into broad clearly depressed necrotic plaques. Morphological and molecular analyses, as well as pathogenicity tests, identified the fungus Colletotrichum orchidophilum (Ascomycota) as the causal agent of the disease. Inoculation tests in controlled conditions confirmed that the two C. orchidophilum strains isolated from fruit lesions are pathogenic on the leaves and fruits of Vanilla planifolia (accessions CR0001 and CR0020). However, these strains induced symptoms only when the epidermis of leaves and fruits had been punctured by a needle. In the absence of injury, no symptom appeared. Colletotrichum arxii and Fusarium proliferatum (Ascomycota) are fungal species that are also frequently isolated from black spot lesions. However, they are not pathogenic to vanilla. This is the first report of C. orchidophilum in Reunion Island. It is also the first demonstration of C. orchidophilum's pathogenicity to an orchid. Simple preventive control measures were proposed to reduce the incidence of black spot disease in vanilla plots. 相似文献
6.
New Host Record of Myrothecium roridum Causing Leaf Spot on Abutilon megapotamicum from China
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Hai‐Yan Ben Wei Gao Hong‐Yun Qu A‐Li Chai Yan‐Xia Shi Xue‐Wen Xie Bao‐Ju Li 《Journal of Phytopathology》2016,164(7-8):563-566
During September 2010, Abutilon megapotamicum plants with dark‐brown concentric spots on leaves were observed in a commercial glasshouse located in Beijing, China. This study was carried out to identify the causal agent of this disease based on Koch's postulates and morphological characteristics. Pathogenicity tests in the glasshouse showed that Myrothecium roridum Tode ex Fr. caused the leaf spot on A. megapotamicum plants, which were the same as those observed in naturally infected plants in the field. Moreover, to confirm the pathogen to species, the rDNA internal transcribed spacer (ITS) of isolate was PCR‐amplified using ITS1 and ITS4 primer pairs and sequenced. DNA analysis revealed a 100% species identity index for M. roridum. To the best of our knowledge, this is the first report of M. roridum on A. megapotamicum in China. 相似文献
7.
Marcelo Rocha Felipe Rossoni Alberto Akama Jansen Zuanon 《Journal of fish biology》2020,96(1):243-250
An expedition to the middle Rio Purus basin uncovered a remarkable new species of the genus Spinipterus. The new species has a very distinct and conspicuous colour pattern resembling a jaguar and it is almost four times larger than Spinipterus acsi, a small specimen (32 mm LS) from Caño Santa Rita, a right bank tributary of Río Nanay in Peru and a second specimen was reported from Rio Juruá, Amazonas State, Brazil. Although the new species is more similar in size and colour pattern to Liosomadoras, it shares the synapomorphies for Spinipterus. The new species differs from the congener by the following characters: (a) colour pattern with large black rosette-like spots over a light yellow to brown background (v. brown background with small dark blotches over the body); (b) adult body size reaching 104.5 mm LS (v. maximum known size 37.1 mm LS); (c) posterior process of cleithrum short, never reaching vertical through the dorsal-fin origin (v. posterior process long, surpassing vertical through the dorsal-fin origin); (d) seven soft pectoral-fin rays (v. six); (e) caudal fin truncated (v. caudal fin rounded). 相似文献
8.
Occurrence of Grey Leaf Spot of Sedum erythrostictum Caused by Cercospora cf. pseudokalanchoes in China
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Yue Yuan Rujun Zhou Haijiao Xu Junfan Fu Ning Hao Hongbo Li 《Journal of Phytopathology》2015,163(11-12):997-1001
Sedum erythrostictum is a perennial herb in the Crassulaceae family, which is a traditional Chinese medicine used for the treatment of hepatitis, dysentery, herpes zoster and swellings. In 2014, a grey leaf spot disease causing significant damage to plants of S. erythrostictum occurred in the Medicinal Herb Garden of Jilin Agricultural University, Jilin Province, China. The fungus mainly infected the leaves. The necrotic lesions on the leaves were circular or elliptical, amphigenous, greyish brown to brown, slightly concave and surrounded by a dark brown distinct margin. The causal agent from symptomatic tissues was identified as Cercospora cf. pseudokalanchoes based on the symptoms, morphological characteristics, molecular identifications and pathogenicity tests. To our knowledge, this is the first formal report of grey leaf spot of S. erythrostictum caused by C. cf. pseudokalanchoes in China. 相似文献
9.
A leaf spot and leaf blight disease was observed on Aloe vera plants as small, circular to oval dark brown necrotic sunken spots on leaves. Infected tissues collected from different sites in diseased fields were cultured on potato carrot agar medium, and the pathogen was identified as Alternaria alternata on the basis of morphological and cultural characteristics. The conidiophores were branched, straight, golden brown, smooth‐walled, measuring up to μm long by 3 μm wide with one conidial scar. The conidia were golden brown in colour and produced in long branched chains, obclavate in shape and in short conical flask. Pathogenicity tests conducted on healthy potted aloe plants in a glasshouse showed typical leaf spot symptoms after 4–7 days. The optimal temperature for the growth of A. alternata was 25°C. 相似文献
10.
Matthew H. Koski Andrea E. Berardi Laura F. Galloway 《Journal of evolutionary biology》2020,33(4):388-400
Colour phenotypes are often involved in communication and are thus under selection by species interactions. However, selection may also act on colour through correlated traits or alternative functions of biochemical pigments. Such forms of selection are instrumental in maintaining petal colour diversity in plants. Pollen colour also varies markedly, but the maintenance of this variation is little understood. In Campanula americana, pollen ranges from white to dark purple, with darker morphs garnering more pollinator visits and exhibiting elevated pollen performance under heat stress. Here, we generate an F2 population segregating for pollen colour and measure correlations with floral traits, pollen attributes and plant‐level traits related to fitness. We determine the pigment biochemistry of colour variants and evaluate maternal and paternal fitness of light and dark morphs by crossing within and between morphs. Pollen colour was largely uncorrelated with floral traits (petal colour, size, nectar traits) suggesting it can evolve independently. Darker pollen grains were larger and had higher anthocyanin content (cyanidin and peonidin) which may explain why they outperform light pollen under heat stress. Overall, pollen‐related fitness metrics were greater for dark pollen, and dark pollen sires generated seeds with higher germination potential. Conversely, light pollen plants produce 61% more flowers than dark, and 18% more seeds per fruit, suggesting a seed production advantage. Results indicate that light and dark morphs may achieve fitness through different means—dark morphs appear to have a pollen advantage whereas light morphs have an ovule advantage—helping to explain the maintenance of pollen colour variation. 相似文献
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12.
Thiago Maia Jorge Luis Badel Michelle Bayer Fernandes Carlos Augusto Dórea Bragança Eduardo Seiti Gomide Mizubuti Sérgio Hermínio Brommonschenkel 《Journal of Phytopathology》2017,165(3):174-188
The Pucciniomycete fungus Hemileia vastatrix causes leaf rust on coffee trees. The pathogen is responsible for considerable yield losses in susceptible coffee cultivars if appropriate management strategies are not implemented. Rapid spread and epidemics of rust fungi are usually associated with the emergence of new races of the pathogen that overcome resistance or with the emergence of more aggressive populations of the pathogen. In Brazil, coffee production is dominated by susceptible cultivars of Coffea arabica and Coffea canephora. We assessed aggressiveness in 46 populations of H. vastatrix from Minas Gerais and Espírito Santo, two of the most important coffee‐producing states in Brazil. We observed a significant difference in the incubation period between the populations from Minas Gerais and Espírito Santo when 183 single‐pustule isolates were inoculated onto Catuaí Vermelho IAC 44, a susceptible C. arabica cultivar. Variation in aggressiveness components was observed between and within localities. Isolates with longer incubation periods also tended to have longer latent periods, although there was only a low correlation between these two aggressiveness components (r2 = 0.34, P = 2.2 × 10?16). Low‐sporulating isolates also had significantly longer incubation and latent periods. The H. vastatrix population from Minas Gerais and Espírito Santo is structured by the formation of groups of individuals with differential level of aggressiveness. Our results indicate that the variation in aggressiveness of the Brazilian H. vastatrix population may be associated with the geographic coffee‐producing areas. 相似文献
13.
Maysa S. Areas Ricardo M. Gonçalves José M. Soman Renate K. Sakate Ricardo Gioria Tadeu A. F. da Silva Júnior Antonio C. Maringoni 《Journal of Phytopathology》2015,163(11-12):1050-1054
Bacterial spot, caused by Xanthomonas spp., is one of the major diseases of pepper in Brazil, causing considerable losses to crop productivity. The efficient management of the disease is hampered by the high variability of the causal agents. In Brazil, there is no knowledge of which species of Xanthomonas occurs on pepper. In this study, 59 strains of Xanthomonas spp. isolated from different pepper‐producing regions of Brazil were characterized by biochemical and molecular techniques. Results showed the prevalence of X. euvesicatoria as the causal agent of bacterial spot on pepper in Brazil. 相似文献
14.
Alexandra Průchová Oldřich Nedvěd Petr Veselý Bára Ernestová Roman Fuchs 《Entomologia Experimentalis et Applicata》2014,151(2):128-134
Previous studies have suggested that spotted patterns are important in the protection of ladybirds against attack by avian predators. Nevertheless, these studies were based on the comparison of several ladybird species differing in colouration, but also in other traits (e.g., chemical protection). We presented natural as well as artificial colour modifications (using brown, red, and black paint) of the ladybird Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) – an invasive alien species for Europe – to an avian predator, the great tit, Parus major L. (Passeriformes: Paridae). All forms were considered to be equal in size, but differed in colouration and in the presence of spots. The chemical protection was equal except for one form. The birds displayed strong avoidance of all forms with red and black colouration; beetles with artificially removed red colouration (painted brown) were attacked more often. The beetles painted brown with black spots were slightly better protected than the painted beetles without spots. We can sum up that spots are of some importance in the protection of ladybirds; nevertheless, red and black colouration is the main part of the visual signal. 相似文献
15.
Graciliano G. A. Santos Bráulio A. Santos Henrique E. M. Nascimento Marcelo Tabarelli 《Biotropica》2012,44(6):771-778
Although tropical forests have been rapidly converted into human‐modified landscapes, tree species response to forest edges remains poorly examined. In this study, we addressed four pioneer tree species to document demographic shifts experienced by this key ecological group and make inferences about pioneer response to forest edges. All individuals with dbh ≥ 1 cm of two short‐lived (Bellucia grossularioides and Cecropia sciadophylla) and two long‐lived species (Goupia glabra and Laetia procera) were sampled in 20 1‐ha forest edge plots and 20 1‐ha forest interior plots in Oiapoque and Manaus, Northeast and Central Amazon, respectively. As expected, pioneer stem density with dbh ≥ 1 cm increased by around 10–17‐fold along forest edges regardless of species, lifespan, and study site. Edge populations of long‐lived pioneers presented 84–94 percent of their individuals in sapling/subadult size classes, whereas edge populations of short‐lived pioneers showed 56–97 percent of their individuals in adult size classes. These demographic biases were associated with negative and positive net adult recruitment of long‐ and short‐lived pioneers, respectively. Our population‐level analyses support three general statements: (1) native pioneer tree species proliferate along forest edges (i.e., increased density), at least in terms of non‐reproductive individuals; (2) pioneer response to edge establishment is not homogeneous as species differ in terms of demographic structure and net adult recruitment; and (3) some pioneer species, particularly long‐lived ones, may experience population decline due to adult sensitivity to edge‐affected habitats. 相似文献
16.
Influence of shell morphometry,microstructure, and thermal conductivity on thermoregulation in two tropical intertidal snails
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Tropical intertidal organisms tolerate large fluctuations in temperature and high desiccation rates when exposed during low tide. In order to withstand the short‐term heat stress, intertidal organisms adopt behavioral responses to maximize their survival. Our previous research showed that tropical littorinids found at the upper and lower intertidal shores in Singapore exhibited different behavioral adaptations during low tide. Most of the upper‐shore Echinolittorina malaccana kept a flat orientation, with the aperture against the substrate and the long axis of the shell towards the sun, whereas a majority of the lower‐shore individuals of Echinolittorina vidua stood with the edge of the aperture perpendicular to the substrate on the rocky shore during low tide. This prompted analyses of the shells of these two species to determine whether the differences in the shell morphometry, microstructure, and thermal conductivity of shells of E. malaccana and E. vidua were associated with their respective behavioral responses to thermal stress. Analyses of shell morphometry and thermal conductivity showed that shells of E. malaccana were more likely to minimize heat gain, despite having a higher thermal conductivity on the outer surface, due to their light‐gray, elongated shell. By contrast, the dark‐colored, globose shells of E. vidua probably gain heat more readily through solar radiation. Scanning electron microscopy images of the shells of both littorinid species further revealed that they have cross‐lamellar structure; however, only individuals of E. vidua showed the presence of disjointed rod layers and a pigmented inner shell surface. Individuals of E. malaccana had a rough outer shell surface with holes that inter‐connect to form water‐trapping channels that probably aid cooling. Individuals of E. vidua, however, had a smooth outer surface with rows of kidney‐shaped depressions as microsculptures which probably help to stabilize shell shape. In both Echinolittorina species, behavioral responses were used to overcome thermal stress during low tide that was associated with shell morphometry and shell thermal conductivity. Such combined adaptations increase survivability of the littorinids at their respective tidal levels. 相似文献
17.
Outbreak of Rhizopus Rot Caused by Rhizopus oryzae on Seedlings of Grafted Cucumber on Pumpkin Rootstock in South Korea
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Jin‐Hyeuk Kwon Dong‐Wan Kang Sang‐Dae Lee Jinwoo Kim 《Journal of Phytopathology》2015,163(7-8):670-674
In 2013, an outbreak of Rhizopus rot caused by Rhizopus oryzae occurred in cucumber grafted onto pumpkin rootstock sampled from seedling farms in Changnyeong, South Korea. A water‐soaked appearance of the affected tissue was the first symptom of this soft fungal rot in the seedling stems of grafted cucumber. Lesions at the graft sites softened and rapidly, rotted, and turned brown or dark brown. Measurements and taxonomic characteristics were most similar to R. oryzae. DNA sequencing and phylogenetic analysis of the internal transcribed spacer rRNA gene region confirmed that the isolates were indeed R. oryzae. Koch's postulates were supported by pathogenicity tests conducted on healthy plants. Based on mycological characteristics, pathogenicity test, and molecular analysis, the causal fungus was identified as R. oryzae Went & Prinsen Geerligs. To our knowledge, this is the first report of Rhizopus rot caused by R. oryzae in seedlings of grafted cucumber on pumpkin rootstock in South Korea. 相似文献
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19.
Sun‐Hee Hong Ji‐Hyun Park Sung‐Eun Cho Hyeon‐Dong Shin 《Journal of Phytopathology》2014,162(5):338-341
Burcucumbers (Sicyos angulatus) showing necrotic leaf spots were found in several locations in Korea during 2008–2012. The causal agent was a fungus that was identified as Cercospora citrullina, based on morphological characteristics as well as sequences of the ribosomal DNA internal transcribed spacer region. Although C. citrullina has been known to attack various cucurbitaceous plants, this is the first report of this fungus on S. angulatus. 相似文献