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1.
Plant rgeneration occurred on leaf-and stem-derived callus of Cuphea ericoides Cham. & Schlechtd obtained in Murashige and Skoog medium supplemented with auxins [indole-3-acetic acid (IAA), -naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-d)] plus cytokinins [6-benzyladenine (BA) or kinetin]. These calluses were subcultured and showed vigorous growth. When subcultured on medium containing 2.22 or 4.44 M BA, the calluses showed profuse regeneration of shoots whereas those subcultured on medium supplemented with 2.69 M NAA or 0.226 M 2,4-d produced numerous roots. Isolated shoots rooted on Murashige and Skoog medium lacking growth regulators or containing 0.54 M NAA or 0.49 M indole-3-butyric acid (IBA). Plantlets were acclimatized to greenhouse conditions.Abbreviations BA
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- MS
Murashige & Skoog medium
- NAA
1--naphthaleneacetic acid 相似文献
2.
Stem explants obtained from a mature tree of Ziziphus mauritiana Lamk were grown on modified Murashige and Skoog medium containing 3800 mg l-1 potassium nitrate, 2475 mg l-1 ammonium nitrate, 11 M benzyladenine and 0.5 M indole-3-acetic acid. During successive subcultures 15–20 shoots per inoculum were produced. Rooting was induced by pretreatment with 50 M indolebutyric acid or 1-naphthaleneacetic acid for 24 h followed by transfer to auxin-free White's medium. Plantlets grew well in a soil and vermiculite mixture.Abbreviations IAA
Indole-3-acetic acid
- NAA
1-naphthaleneacetic acid
- BA
benzyladenine
- MS
Murashige and Skoog 相似文献
3.
Plants were regenerated from leaflet-derived callus of Aeschynomene sensitiva, A. americana and A. villosa. Explants were induced to form callus when aseptically cultured on Murashige and Skoog medium solidified with 0.8% agar and containing 0.5 or 0.05 M naphthaleneacetic acid and 4.4 or 13.3 M benzyladenine. Shoot regeneration was readily achieved. Roots were induced when shoots were transferred to medium devoid of growth regulators or with 0.05, 0.5 or 5.4 M naphthaleneacetic acid. Plantlets were successfully transplanted to soil. Callus from A. falcata failed to regenerate shoots. Explants from leaflets of A. fluminensis did not produce callus when cultured in vitro.Abbreviations BA
benzyladenine
- MS
Murashige and Skoog (1962) medium
- NAA
naphthaleneacetic acid 相似文献
4.
J. L. Vandemoortele J. P. Billard J. Boucaud Th. Gaspar 《Plant Cell, Tissue and Organ Culture》1996,44(1):25-30
A micropropagation protocol of parsley,Petroselinum crispum (Mill.) Nyman (curled type) has been developed. Surface-sterilized axillary buds cultured on Murashige and Skoog medium supplemented with benzyladenine, kinetin, or thidiazuron developed axillary shoots (rosettes). Kinetin resulted in only a low proliferation rate. The concentrations of thidiazuron or benzyladenine that were optimal for shoot proliferation, resulted in shoots with a low capability to root. During the rooting treatment, these shoots showed wilting signs. Rooting was increased significantly by using a two-week inductive stage with 2.5 M naphthaleneacetic acid directly followed by acclimatization. Two proliferation media (5 M benzyladenine and 0.5 M naphthaleneacetic acid or 5 M kinetin and 2.5 M naphthaleneacetic acid) resulted in moderate proliferation but produced shoots that were easy-to-root. These media have been tested by repeated axillary proliferation on the same medium. The medium with 5 M benzyladenine and 0.5 M naphthaleneacetic acid was optimal.Abbreviations BA
6-Benzyladenine
- IBA
Indole-3-butyric acid
- MS
Murashige & Skoog
- NAA
-Naphthaleneacetic acid 相似文献
5.
Massimo H. M. Sanago Vern I. Shattuck Judith Strommer 《Plant Cell, Tissue and Organ Culture》1996,45(2):165-168
A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 M thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 M -naphthaleneacetic acid or 1.0–2.0 M indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.Abbreviations BAP
6-benzyladenine
- 2,4-d
2,4-dichlorophenoxy acetic acid
- GA3
gibberellic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic acid
- TDZ
thidiazuron (N-phenyl-N-1,2,3-thiadiazol-5-ylurea) 相似文献
6.
Leontochir ovallei Phil., an endangered Chilean species in the Alstroemericeae, was micropropagated on Murashige & Skoog medium supplemented with 4 M benzyladenine, 1 M indolebutyric acid and 146 mg l-1 glutamine. Over 88% of the shoots rooted in vitro when treated with 10 M naphthaleneacetic acid and micropropagated plantlets were successfully transplanted into the greenhouse.Abbreviations BA
benzyladenine
- IBA
indolebutyric acid
- 2iP
isopentenyladenine
- NAA
naphthaleneacetic acid
- MS
Murashige and Skoog (1962) medium 相似文献
7.
Xanthium strumarium L. was micropropagated by rooting shoots proliferated from shoot-tip explants. The best shoot proliferation was obtained from explants growing on Murashige and Skoog medium supplemented with 4.4 to 8.9 M benzyladenine (BA) and 1.1 to 2.1 M naphthaleneacetic acid (NAA). The micropropagated plants were transferred to potting media and maintained under high humidity conditions in the greenhouse. The media that produced best shoot proliferation from shoot-tip explants also produced the most callus from hypotocotyl, cotyledon and shoot-tip explants, whereas more callus was produced on leaf explants with a lower BA concentration (1.1 M) and 1.1 M NAA.Abbreviations BA
benzyladenine, 2 4-d-2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- NAA
naphthaleneacetic acid
- MS
Murashige and Skoog
Technical contribution No. 3319 of the South Carolina Agricultural Experiment Station, Clemson University. 相似文献
8.
Explants from aseptically germinated seeds of Thymus piperella L. were induced to form shoots on modified Murashige and Skoog medium, the best yield being 5.1 shoots per explant when the medium contained 6.6 M BA plus 2.8 M IAA. Shoots could be rooted on the same basal medium supplemented with 2.8 M IAA, and 71% of the plantlets were successfully acclimatized.Abbreviations BA
benzyladenine
- CMS
modified MS culture medium
- IAA
indoleacetic acid
- MS
Murashige & Skoog (1962) culture medium
- NAA
-naphthaleneacetic acid 相似文献
9.
Plant regeneration via somatic embryogenesis in ginger 总被引:5,自引:0,他引:5
A. Kackar S. R. Bhat K. P. S. Chandel S. K. Malik 《Plant Cell, Tissue and Organ Culture》1993,32(3):289-292
Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 M was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved when embryogenic cultures were transferred to Murashige & Skoog medium containing 8.9 M benzyladenine. Histological studies revealed various stages of somatic embryogenesis characteristic of the monocot system. The in vitro-raised plants have been established in soil.Abbreviations BA
benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog
- NAA
naphthaleneacetic acid 相似文献
10.
A method for long-term plant regeneration of Phaseolus coccineus L, is described. Shoot-tips and cotyledonary nodes cultured on a Murashige and Skoog medium supplemented with N6-benzylaminopurine, 10 M, and -naphthaleneacetic acid, 1M, formed multiple bud-shoots. These shoots were transferred to medium containing BAP 1 M, NAA 0.1 M, and gibberellic acid 3 M to promote shoot growth and further shoot multiplication. Rooting was achieved in medium with 11 M indole-3-acetic acid. Rooted plants grew to maturity and were fertile. Cultures have maintained their ability to regenerate plants for more than two years. A sample of 30 regenerated plants (R0) was tested for chromosome number, all of them being diploid; seven isozymatic systems were electrophpretically analyzed in 82 R0 regenerated plants. No differences were observed in their electrophoretic patterns in comparison with those shown by seedlings. Histological studies revealed the origin of buds from calluses via organogenesis.Abbreviations BAP
N6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic acid
- ADH
alcohol dehydrogenase
- GOT
glutamic-oxaloacetic transaminase
- MDH
malate dehydrogenase
- 6PGD
6-phosphogluconate dehydrogenase
- PGI
Phosphoglucose isomerase
- PGM
phosphoglucose mutase
- SK
shikimate dehydrogenase 相似文献
11.
Initiation and propagation of Glycine max L. Merr.: Plants from tissue-cultured epicotyls 总被引:1,自引:0,他引:1
M. S. Wright M. H. Williams P. E. Pierson M. G. Carnes 《Plant Cell, Tissue and Organ Culture》1987,8(1):83-90
A successful technique for the initiation and proliferation of shoots from epicotyl tissue of soybean, Glycine max, has been developed and is described. Fertile plants were recovered. Seeds were germinated on Murashige and Skoog medium containing 5 M benzyladenine. Explanted epicotyl sections were induced to form callus and shoots on Schenk and Hildebrandt medium containing 5.2 mM monobasic ammonium phosphate, 74 M 3-aminopyridine, and 20 M kinetin for five weeks. Shoot proliferation was maintained on N6 medium containing 1.75 mM ammonium sulfate, 2.1 nM picloram, and 0.1 M benzyladenine. Shoots rooted on Gamborg's B5 medium without growth regulators. Shoot-forming cultures were maintained for 60 months. Although all varieties tested produced shoots, some variation in numbers of shoots obtained was observed. 相似文献
12.
Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower 总被引:4,自引:0,他引:4
A. K. A. Mandal A. K. Chatterji S. Dutta Gupta 《Plant Cell, Tissue and Organ Culture》1995,43(3):287-289
Somatic embryos were induced directly on adaxial surface of cotyledonary leaves within 8–10 days of culture on Murashige and Skoog medium containing 5.37 to 10.74 M 1 — napthaleneacetic acid and 2.22 M benzyl adenine. Germinated embryos with shoot axes developed into complete plants after transfer onto half stength Murashige and Skoog medium containing 1.07 M 1 — napthaleneacetic acid. Histological studies suggested direct origin of somatic embryos with broad-base attachment.Abbreviations BA
benzyl adenine
- MS
Murashige and Skoog
- NAA
1-napthaleneacetic acid
- RH
relative humidity 相似文献
13.
Micropropagation of Actinidia kolomikta 总被引:3,自引:0,他引:3
Jaroslav Kovae 《Plant Cell, Tissue and Organ Culture》1993,35(3):301-303
Nodal segments of female A. kolomikta shoots were cultured on Murashige and Skoog modified medium with different growth regulator concentrations. The highest multiplication rate (9.5) was achieved on a medium with 10 M benzyladenine and 0.1 M indolebutyric acid. Over 90% of shoots rooted in vivo after pulse stimulation with indolebutyric acid.Abbreviations BA
benzyladenine
- IBA
indole-3-butyric acid
- NAA
1-naphthaleneacetic acid 相似文献
14.
A procedure for rapid in vitro propagation of the aromatic and medicinal plant Hemidesmus indicus (L.) R.Br. (Family Asclepiadaceae) from nodal explants is described. The highest shoot multiplication rate of 8.2 ± 0.4 shoots/explant with a 95% frequency was achieved in S weeks culture period on Murashige and Skoog medium supplemented with 1.15 M kinetin and 0.054 M -naphthaleneacetic acid. Excised shoots were rooted on the same basal medium supplemented with 1.15 M kinetin and 7.35 M indole-3-butyric acid. Shoots derived from subcultures exhibited better rooting response than those from primary cultures. After a hardening phase of 2 weeks, there was a 70% transplantation success in the field.Abbreviations MS
Murashige and Skoog (1962) medium
- BA
N6 benzyladenine; KN kinetin
- NAA
a-naphthaleneacetic acid
- IBA
indole-3-butyric acid
- IAA
indole-3-acetic acid 相似文献
15.
Factors effecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga) 总被引:2,自引:0,他引:2
A procedure for adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga Mill.) using thidiazuron (TDZ) was developed. Excised leaves of cultures grown on Murashige and Skoog (MS) medium containing 5 M benzyladenine (BA) and 0.9% Gibco Phytagar were used. Several experiments were conducted to determine optimum concentrations of thidiazuron, -naphthaleneacetic acid (NAA) and sucrose. When the medium contained 1.5 M TDZ and 2.5 M NAA, 85% of the discs regenerated shoots with an average of eight shoots per leaf disc. An incubation period of three weeks in the dark was necessary for optimum shoot regeneration. Leaves excised from four to six-week-old cultures gave a higher percent shoot regeneration than leaves from cultures older than six weeks. Regeneration percentages were significantly reduced when sucrose concentration in the medium was less than 3%. A significantly higher percentage of shoots regenerated when leaf discs were placed on the regeneration medium abaxial side down as compared to the adaxial side.Regenerated shoots were cultured on MS medium containing 5 M BA and rooted on half-strength MS medium containing 10 M NAA. Rooted plantlets were acclimatized to greenhouse conditions for evaluation of any somaclonal variation. The importance of these findings are discussed in relation to in vitro improvement of plants.Abbreviations BA
benzyladenine
- MS
Murashige & Skoog (1962) salt mixture
- NAA
-naphthaleneacetic acid
- TDZ
thidiazuron (N-phenyl-N'-1,2,3,-thiadiazol-5-ylurea)
Approved for publication by the Director, West Virginia Agric. and For. Expt. Sta. as Scientific Article No.2346 相似文献
16.
I. O. Ahn Bui Van Le C. Gendy K. Tran Than Van 《Plant Cell, Tissue and Organ Culture》1996,45(3):237-243
Direct somatic embryos were differentiated on cotyledon transverse Thin Cell Layers (tTCLs) of Panax ginseng after 9 weeks in the Murashige and Skoog basal (MS) medium containing 2,4-d (5M). When MS medium containing 2,4-d (5M) was used for seedling pretreatment and for tTCLs culture, somatic embryos were observed 2 weeks earlier, i.e. after 7 weeks of culture. On the tTCLs from seedlings pretreated with 2,4-d (5M) combined with benzyladenine and zeatin at 0.1 M (BZ), somatic embryos were observed after 6 weeks of culture and the percentage of embryogenesis was higher (62%) than when 2,4-d was used alone for pretreatment (40%). Similar results were also obtained from pretreatment with combinations of 2,4-d (5M) and thidiazuron (TDZ) (0.01, 0.1M). When a combination of 2,4-d (5M) and BZ (0.1M) was used both for seedling pretreatment and for tTCLs culture, both somatic embryos and shoots were observed after only 3 weeks. As the concentration of BZ increased, the percentage of somatic embryogenesis decreased but the percentage of organogenesis increased. Similar responses were obtained with a combination of 2,4-d (5M) and TDZ (0.01M). On the medium containing both NAA (0.3M) and BZ (1M), globular- and heart- stage embryos developed after 4 weeks of culture into cotyledonary-staged embryos which remained dormant after a short elongation of the embryo axis. The importance of seedling pretreatment by growth substances in enhancing somatic embryogenesis is reported.Abbreviations BA
6-benzyladenine
- BZ
combination of BA and zeatin
- 2,4-d
2,4-dichlorophenoxyacetic acid
- MS medium
Murashige and Skoog basal medium
- NAA
a-naphthaleneacetic acid
- TDZ
thidiazuron
- tTCLs
transverse thin cell layers
- TCL
longitudinal thin cell layer 相似文献
17.
A system for in vitro clonal propagation has been developed in tea plants. Shoots obtained from primary explants were induced from terminal buds and axillary buds of mature field-grown plants. Cultures were initiated from both types of explants on Murashige and Skoog (MS) medium supplemented with 10% coconut milk (CM), 200 mg l-1 of yeast extract (YE), 1.4 M indoleacetic acid (IAA) and 17.8 M benzyladenine (BA). The shoot tips were multiplied on 1/2 strength MS medium containing 10% CM, 2.9 M IAA and 17.8 M BA. The larger shoots were separated after multiplication and rooted on 1/2 MS medium supplemented with 11.4 M ascorbic acid and 34.5 M indolebutyric acid (IBA). A pretreatment of the plants with an aqueous solution of 493 M IBA greatly increased the frequency of rooting. More than 60% of the rooted plants have been transferred to soil successfully.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- YE
yeast extract
- CM
coconut milk
- MS
Murashige and Skoog medium (1962) 相似文献
18.
Miguel Carlos Sánchez-Gras Maria del Carmen Calvo 《Plant Cell, Tissue and Organ Culture》1996,45(3):259-261
Cultures of Lavandula latifolia Medicus were established from axillary buds of mature field-grown plants. Explants were initially cultured on media with two different macronutrient combinations and benzyladenine or kinetin added either individually or with naphthaleneacetic acid. Subsequently, explants were subcultured in Murashige and Skoog medium supplemented with 20% coconut milk, 0.57 M indoleacetic acid and 8.88 M benzyladenine. Shoot proliferation from axillary buds was not affected by seasonal fluctuations in the stock plants but depended on the macronutrient composition and on the type and concentration of cytokinin tested. Best results were obtained in explants initially cultured in media with Murashige and Skoog constituents and supplemented with 5 M benzyladenine. In vitro-grown shoots were used to induce multiple shooting by transferring them to subculture medium. Shoots were rooted on Murashige and Skoog medium with macronutrients at half-strength. Plantlets were transferred to soil and grown to maturity. 相似文献
19.
Axillary shoot induction and plant regeneration were obtained in Plantago ovata. The optimum medium for inducing axillary shoots was Murashige & Skoog (MS) medium [5] supplemented with 4.6 M kinetin and 0.05 M NAA. Rooting of shoots was best on half-strength MS medium containing 5.0 M IBA and 0.05 M kinetin. The regenerated plants were similar to the control plants in karyotypic and phenotypic details. 相似文献
20.
M. Hossain M. R. Karim R. Islam O. I. Joarder 《Plant Cell, Tissue and Organ Culture》1993,34(2):199-203
A protocol for organogenesis from nucellar explants excised from fertilized ovules of immature fruits of Aegle marmelos Corr. was developed. Adventitious buds were initiated on Murashige and Skoog's (MS) medium containing various combinations of 6-benzyladenine (BA), -naphthalene-acetic acid (NAA), 3-indoleacetic acid and gibberellic acid. Medium containing 4.4 m BA and 2.7 M NAA produced the maximum number of adventitious buds per explant. Shoots were elongated by transferring explants with shoot buds to medium with a low concentration of BA (0.44 M). Rooting of in vitro-regenerated shoots was obtained in half-strength MS medium with 4.9 M indole-3-butyric acid. This is the first report of plant regeneration from nucellar explants of A. marmelos.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- NAA
naphthaleneacetic acid
- GA3
gibberellic acid 相似文献