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1.
Study of cell wall growth in Bacillus megaterium by high-resolution autoradiography. 总被引:13,自引:10,他引:3
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Growth of the cell wall of Bacillus megaterium was studied by pulse-labeling the cell wall of a DAP- Lys- mutant for a very short time with tritium-labeled diaminopimelic acid. The distribution of radioactivity along the cell wall was examined by high-resolution autoradiography on isolated cell walls and thin sections of bacteria. The results indicate that cell wall elongation occurs by diffuse intercalation of newly synthesized murein into the expanding cell wall during exponential growth, as well as during germination, and that the only zone of highly localized diaminopimelic acid incorporation is found at the cross wall during its synthesis. This zone contains about 30% of the radioactivity incorporated into the cell wall. Analysis of autoradiographs of thin sections of bacteria shows that the total radioactivity incorporated per bacterium doubles during the life cycle. This doubling occurs in the cylindrical part of the cell wall but not in the polar caps. This seems to indicate that elongation of the bacterium is not constant during the life cycle but increases with the length of the cell. 相似文献
2.
Escherichia coli 173-25, whose cell wall was labelled with14C-diaminopimelic acid, was found to lose about 15% radioactivity during growth in a fresh medium, two thirds or more being
lost during the first two generations. Degradation products of the cell wall were mostly of low-molecular type. About 5% of
the cells lyzed as a result of transfer associated with filtration, washing and resuspension of the bacterial population in
a diaminopimelic acid (DAP) deficient medium. The degradation was very low during the first 20 min. The amount of wall material
released from the cells increased between 20–30 min and a sudden decrease of viability of the population was observed. The
degradation of murein triggered by starvation for DAP continued when supplementing the deficient medium with DAP and when
growth was resumed. About one-half of the cell wall material released into the medium under these conditions was macromolecular.
However, lysis of the cells and release of proteins into the medium were rapidly interrupted after DAP was added to the starving
culture and the differential rate of synthesis of the cell wall increased. Turnover of murein was not associated with protein
turnover. 相似文献
3.
The variable T model for gram-negative morphology 总被引:11,自引:0,他引:11
Gram-negative micro-organisms possess only a very thin murein sacculus to resist the stress caused by the internal hydrostatic pressure. The sacculus consists of at most one molecular layer of peptidoglycan in an extended conformation. It must grow by the insertion and cross-linking of new murein to the old before the selective cleavages of the stress-bearing murein are made which allow wall enlargement. Since insertion of new murein occurs all over the surface of Escherichia coli (even in completed poles), the internal pressure would tend to force the cells into a spherical shape and prevent both cylindrical elongation and cell division. Of course, Gram-negative bacteria do achieve a variety of shapes and do divide. Because prokaryote cells, unlike eukaryotic cells, do not have cytoskeletons and contractile proteins to transduce biochemical free energy into the mechanical work needed to achieve aspherical shapes and to divide, this paradox seems to be resolvable only by postulating that the details of the biochemical mechanism for wall growth vary in different regions of the surface, affecting the work required to enlarge the wall locally. Depending on the degree and rate of change in the biochemical energetics, it is possible to account for rod and the other more complex shapes of Gram-negative bacteria. Division occurs in Gram-negative organisms by the development of constrictions that progressively invade the cytoplasm. The work to cause these morphological processes must ultimately derive from the biochemical process of the stress-bearing wall formation. A biophysical basis for cell division in these prokaryotic organisms is proposed. 相似文献
4.
tetaine, a low molecular weight broad spectrum antibiotic of aminoacid derivative type and of low toxicity, is an inhibitor of cell wall synthesis in K-12 3000 HFr strain. Tetaine inhibits the incorporation of 3H DAP and 3H L-Ala to murein of E. coli. In the presence of tetaine nucleotide precursors of murein synthesis were radioactive mainly due to the 3H uridine and not 14C alanine, what indicates the lack of incorporation of alanine to UDP-Mur-NAc. It is postulated that tetaine inhibits the synthesis of murein nucleotide precursors at the level of incorporation of first alanine moiety. 相似文献
5.
Multiple deletions in murein hydrolases revealed that predominantly amidases are responsible for cleavage of the septum during cell division. Endopeptidases and lytic transglycosylases seem also be involved. In the absence of these enzymes E. coli grows normally but forms chains of adhering cells. Surprisingly, mutants lacking up to eight different murein hydrolases still grow with almost unaffected growth rate. Therefore it is speculated that general enlargement of the murein sacculus may differ from cell division by using transferases rather than the two sets of hydrolytic and synthetic enzymes as seems to be the case for the constriction process. A model is presented that describes growth of the murein of both Gram-positive and -negative bacteria by the activity of murein transferases. It is speculated that enzymes exist that catalyze a transpeptidation of the pre-existing murein onto murein precursors or nascent murein by using the chemical energy present in peptide cross-bridges. Such enzymes would at the same time cleave bonds in the murein net and insert new material into the growing sacculus. 相似文献
6.
Bacillus megaterium sporal peptidoglycan synthesis studied by high-resolution autoradiography 总被引:1,自引:1,他引:0
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Cells of a Dap- Lys- mutant strain of Bacillus megaterium were pulse labeled with [3H]diaminopimelic acid at different times of growth and sporulation. They were processed for radioactivity measurements and high-resolution autoradiography either just after the pulse or after a chase in a nonradioactive medium until refractile forespores started to appear at time (t)4,5. In the pulse-labeled cells, autoradiographs and radioactivity measurements showed that the radioactivity incorporated during a pulse decreased abruptly after t0 and stayed at a low level until t5, although the forespore wall and cortex were formed between t4 and t5. In the pulse-chased bacteria, the acid-insoluble radioactivity, as well as the number of silver grains on autoradiographs, increased during the chase in cells labeled at t1 to t2, whereas it decreased in those labeled before t0. Furthermore, analysis of silver grain distribution showed that, in stage IV bacteria, grains were distributed at the outside of the forespore, mostly on the sporangium cell wall, when pulse-labeling occurred before or at t0; they were located along the cortex and in the forespore cytoplasm when labeling was made at t1 or t2. These facts show that [3H]diaminopimelic acid necessary for spore envelope synthesis was incorporated before their morphological appearance. Free or small diaminopimelic acid precursors entered the sporangium between t1 and t2. The appearance of silver grains in the forespore cytoplasm suggests that the forespore is implicated in sporal peptidoglycan synthesis. 相似文献
7.
In vivo metabolism of 2,2'-diaminopimelic acid from gram-positive and gram-negative bacterial cells by ruminal microorganisms and ruminants and its use as a marker of bacterial biomass.
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Cells of Bacillus megaterium GW1 and Escherichia coli W7-M5 were specifically radiolabeled with 2,2'-diamino[G-3H]pimelic acid ([3H]DAP) as models of gram-positive and gram-negative bacteria, respectively. Two experiments were conducted to study the in vivo metabolism of 2,2'-diaminopimelic acid (DAP) in sheep. In experiment 1, cells of [3H]DAP-labeled B. megaterium GW1 were infused into the rumen of one sheep and the radiolabel was traced within microbial samples, digesta, and the whole animal. Bacterially bound [3H]DAP was extensively metabolized, primarily (up to 70% after 8 h) via decarboxylation to [3H]lysine by both ruminal protozoa and ruminal bacteria. Recovery of infused radiolabel in urine and feces was low (42% after 96 h) and perhaps indicative of further metabolism by the host animal. In experiment 2, [3H]DAP-labeled B. megaterium GW1 was infused into the rumens of three sheep and [3H]DAP-labeled E. coli W7-M5 was infused into the rumen of another sheep. The radioactivity contents of these mutant bacteria were insufficient to use as tracers, but the metabolism of DAP was monitored in the total, free, and peptidyl forms. Free DAP, as a proportion of total DAP in duodenal digesta, varied from 0 to 9.5%, whereas peptidyl DAP accounted for 8.3 to 99.2%. These data reflect the extensive metabolism of bacterially bound DAP within the gastrointestinal tracts of ruminant animals and serve as a serious caution to the uncritical use of DAP as a marker of bacterial biomass in the digesta of these animals. 相似文献
8.
Evidence for diffuse growth of the cylindrical portion of the Escherichia coli murein sacculus. 总被引:16,自引:7,他引:9
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High-resolution autoradiography of thin sections of Escherichia coli cells whose murein was pulse-labeled with [3H]diaminopimelic acid after a period of diaminopimelic acid deprivation indicated that elongation of the murein sacculus occurs by a multisite (diffuse) process. Upon chasing, radioactivity in polar murein was stable, whereas radioactivity in cylindrical murein was reduced, indicating that diffuse intercalation of new murein occurred during cell elongation. Elongation and septation were shown to be overlapping processes. 相似文献
9.
10.
Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli 总被引:27,自引:0,他引:27
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Joachim-Volker Hltje 《Microbiological reviews》1998,62(1):181-203
To withstand the high intracellular pressure, the cell wall of most bacteria is stabilized by a unique cross-linked biopolymer called murein or peptidoglycan. It is made of glycan strands [poly-(GlcNAc-MurNAc)], which are linked by short peptides to form a covalently closed net. Completely surrounding the cell, the murein represents a kind of bacterial exoskeleton known as the murein sacculus. Not only does the sacculus endow bacteria with mechanical stability, but in addition it maintains the specific shape of the cell. Enlargement and division of the murein sacculus is a prerequisite for growth of the bacterium. Two groups of enzymes, hydrolases and synthases, have to cooperate to allow the insertion of new subunits into the murein net. The action of these enzymes must be well coordinated to guarantee growth of the stress-bearing sacculus without risking bacteriolysis. Protein-protein interaction studies suggest that this is accomplished by the formation of a multienzyme complex, a murein-synthesizing machinery combining murein hydrolases and synthases. Enlargement of both the multilayered murein of gram-positive and the thin, single-layered murein of gram-negative bacteria seems to follow an inside-to-outside growth strategy. New material is hooked in a relaxed state underneath the stress-bearing sacculus before it becomes inserted upon cleavage of covalent bonds in the layer(s) under tension. A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template. 相似文献
11.
Peptidoglycan turnover during growth recovery after chloramphenicol treatment in a Dap-Lys-mutant of Bacillus megaterium 总被引:2,自引:0,他引:2
The study of diaminopimelic acid (DAP) incorporation and turnover during growth recovery in chloramphenicol-treated (CMP-treated) Bacillus megaterium cells showed that two kinds of turnover occurred. A low acid-soluble turnover appeared as soon as growth resumed in bacteria labeled before the CMP treatment and at the middle of the first generation in those labeled during the treatment. The acid-insoluble turnover appeared only at the beginning of the second generation of growth resumption in bacteria labeled before CMP addition and at the beginning of the third generation in those labeled during the CMP treatment. The acid-soluble release observed during the period of cell wall thinning is too low to account for the decrease of the wall thickness and the acid-insoluble loss appears after this period. When bacteria were transferred into partially spent medium instead of fresh culture medium the acid-insoluble release started to appear half a generation sooner. Electron microscopic observations showed that in this case, large scales detached from the cell wall. This activity of wall degradation was not observed when the partially spent medium was previously heated for 10 min at 100 degree C. The persistence of a thick wall on cell ends during the first generation does not reflect an absence of growth sites because their labeling on autoradiographs is high. Rather, it seems to be due to a low lytic activity at the poles. 相似文献
12.
We have studied the mechanisms by which amino acid starvation of Escherichia coli induces resistance against the lytic and bactericidal effects of penicillin. Starvation of E. coli strain W7 of the amino acids lysine or methionine resulted in the rapid development of resistance to autolytic cell wall degradation, which may be effectively triggered in growing bacteria by a number of chemical or physical treatments. The mechanism of this effect in the amino acid-starved cells involved the production of a murein relatively resistant to the hydrolytic action of crude murein hydrolase extracts prepared from normally growing E. coli. Resistance to the autolysins was not due to the covalently linked lipoprotein. Resistance to murein hydrolase developed most rapidly and most extensively in the portion of cell wall synthesized after the onset of amino acid starvation. Lysozymes digests of the autolysin-resistant murein synthesized during the first 10 min of lysine starvation yielded (in addition to the characteristic degradation products) a high-molecular-weight material that was absent from the lysozyme-digests of control cell wall preparations. It is proposed that inhibition of protein synthesis causes a rapid modification of murein structure at the cell wall growth zone in such a manner that attachment of murein hydrolase molecules is inhibited. The mechanism may involve some aspects of the relaxed control system since protection against penicillin-induced lysis developed much slower in amino acid-starved relaxed controlled (relA) cells than in isogenic stringently controlled (relA+) bacteria. 相似文献
13.
A defect in cell wall recycling triggers autolysis during the stationary growth phase of Escherichia coli.
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The first gene of a family of prokaryotic proteases with a specificity for L,D-configured peptide bonds has been identified in Escherichia coli. The gene named ldcA encodes a cytoplasmic L, D-carboxypeptidase, which releases the terminal D-alanine from L-alanyl-D-glutamyl-meso-diaminopimelyl-D-alanine containing turnover products of the cell wall polymer murein. This reaction turned out to be essential for survival, since disruption of the gene results in bacteriolysis during the stationary growth phase. Owing to a defect in muropeptide recycling the unusual murein precursor uridine 5'-pyrophosphoryl N-acetylmuramyl-tetrapeptide accumulates in the mutant. The dramatic decrease observed in overall cross-linkage of the murein is explained by the increased incorporation of tetrapeptide precursors. They can only function as acceptors and not as donors in the crucial cross-linking reaction. It is concluded that murein recycling is a promising target for novel antibacterial agents. 相似文献
14.
Inside-to-outside growth and turnover of the wall of gram-positive rods 总被引:17,自引:0,他引:17
Gram-positive, rod-shaped bacteria, given a pulse of peptidoglycan precursors, first exhibit a lag before the second or turnover phase of peptidoglycan commences. This is because new material is inserted on the inner face of the wall and gradually displaced through the wall. Based on this experimental observation, a mathematical model was constructed and compared with experimental data obtained in several laboratories for the first and second phases of wall turnover of Bacillus subtilis. The model allows the parameters of the process to be estimated for experiments with any labeling time. According to the surface stress theory the wall which is layed down immediately outside the cytoplasmic layer is in an unextended conformation. As subsequent additions of murein occur, the wall moves outward, becomes stretched, and bears the stress due to hydrostatic pressure. Ultimately, peptide and glycosyl bonds become cleaved. At the end of the lag phase the cleavage becomes so extensive that wall fragments are liberated into the medium. This strategy permits rod-shaped growth. In some experimental situations the half-life of wall radioactivity in this second phase roughly equals the doubling time; consequently, the exponential release probably does not represent random turnover but instead is the result of expansion of the underlying wall that continues to create strain which favors autolysis action. The slower turnover of the third phase, where there is a much slower loss, is also included in the analysis. 相似文献
15.
Diaminopimelic acid (DAP) uptake was studied in Bacillus megaterium. The K m and V max were determined for bacteria grown with or without DAP. The uptake of DAP was shown to be constitutive and unaffected by the presence of other amino acids (including cystine). The concentration of DAP and lysine in the amino acid pool was examined and a procedure for pulse-labelling cell walls developed. 相似文献
16.
Mode of Cell Wall Growth of Bacillus megaterium 总被引:13,自引:11,他引:2
The growth of the cell wall of Bacillus megaterium KM was examined by labeling the cell wall uniformly with (3)H-alpha, epsilon-diaminopimelic acid and examining by radioautography the distribution of labeled cell wall material in daughter cells. The results indicate that the label is distributed uniformly to all daughter cells and rule out a single growth point, either central or apical, for these cells. The results strongly suggest that cell wall growth occurs by uniform deposition of new wall over the whole cell surface. 相似文献
17.
Park JT 《Journal of bacteriology》2001,183(13):3842-3847
Turnover and recycling of the cell wall murein represent a major metabolic pathway of Escherichia coli. It is known that E. coli efficiently reuses, i.e., recycles, its murein tripeptide, L-alanyl-gamma-D-glutamyl-meso-diaminopimelate, to form new murein. However, the question of whether the cells also recycle the amino sugar moieties of cell wall murein has remained unanswered. It is demonstrated here that E. coli recycles the N-acetylglucosamine present in cell wall murein degradation products for de novo murein and lipopolysaccharide synthesis. Furthermore, E. coli also recycles the anhydro-N-acetylmuramic acid moiety by first converting it into N-acetylglucosamine. Based on the results obtained by studying mutants unable to recycle amino sugars, the pathway for recycling is revealed. 相似文献
18.
The cell wall, a major barrier protecting cells from their environment, is an essential compartment of both bacteria and archaea.
It protects the organism from internal turgor pressure and gives a defined shape to the cell. The cell wall serves also as
an anchoring surface for various proteins and acts as an adhesion platform for bacteriophages. The walls of bacteria and archaea
are mostly composed of murein and pseudomurein, respectively. Cell wall binding domains play a crucial role in the non-covalent
attachment of proteins to cell walls. Here, we give an overview of the similarities and differences in the biochemical and
functional properties of the two major murein and pseudomurein cell wall binding domains, i.e., the Lysin Motif (LysM) domain
(Pfam PF01476) and the pseudomurein binding (PMB) domain (Pfam PF09373) of bacteria and archaea, respectively. 相似文献
19.
Helmut König 《当今生物学》2016,46(6):374-383
Cell wall types of Bacteria and Archaea The acaryote microorganisms are divided into the two domains Bacteria and Archaea. The third domain represent the Eukarya. There is no universal cell wall polymer found in all Bacteria and Archaea. Due to their morphology several cell wall types can be identified, but the chemical diversity of the individual polymers is considerably greater. Certain cell wall polymers are limited to one of the two domains of Bacteria or Archaea like the murein of the Bacteria or the pseudomurein of some methanogens. Peptidoglycans (murein, pseudomurein) do not occur in eukaryotes. On the other hand individual cell wall polymers possess similarities to polymers of other domains. The structural principle of the methanochondroitin is also implemented in the eukaryotic connective tissue. The cell wall polymers consist frequently of glycoconjugates in which the amino acid content (glycoproteins) or the glycan moiety (proteoglycan‐like polymers) predominate. Both components (carbohydrates, amino acids) can also occur in similar amounts (peptidoglycan). There exist also cell wall polymers, which consist only of glycans (slimes, methanochondroitin) or amino acids (proteins, poly‐γ‐D‐glutamyl polymers). Cell wall‐free species (Mycoplasma) also occur. The chemical composition of the cell surface polymers was one of the first phenotypic characteristics that supported the 16 sRNA concept of Carl Woese to assign acaryote organisms into the two domains Bacteria and Archaea. A common feature of all Archaea is the lack of muramic acid and an outer membrane. The later occurs in the gramnegative Bacteria. During the evolution of Bacteria and Archaea a great variety of chemically different cell wall polymers has been developed which allow the growth and interaction of Bacteria and Archaea in different habitats. In this paper, some important surface polymers of Bacteria and Archaea are presented according to their chemical composition. 相似文献
20.
Uptake of cell wall peptides by Salmonella typhimurium and Escherichia coli. 总被引:21,自引:9,他引:12
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During bacterial growth, cell wall peptides are released from the murein and reused for the synthesis of new cell wall material. Mutants defective in peptide transport were unable to reutilize cell wall peptides, demonstrating that these peptides are taken up intact into the cytoplasm prior to reincorporation into murein. Furthermore, cell wall peptide recycling was shown to play an important physiological role; peptide transport mutants which were unable to recycle these peptides showed growth defects under appropriate conditions. Using mutants specifically defective in each of the three peptide transport systems, we showed that the uptake of cell wall peptides was mediated solely by the oligopeptide permease (Opp) and that neither the dipeptide permease (Dpp) nor the tripeptide permease (Tpp) played a significant role in this process. Our data indicate that the periplasmic oligopeptide-binding protein has more than one substrate-binding site, each with different though overlapping specificities. 相似文献