响应面优化伏毛铁棒锤总生物碱微波辅助提取工艺

目的：优化微波辅助提取伏毛铁棒锤总生物碱工艺,并比较不同产地伏毛铁棒锤中总生物碱含量。方法：以单因素试验为基础,结合响应面法,以总生物碱的含量为评价指标,考察温度、料液比、时间和体积分数对提取得率的影响。结果：最佳微波辅助提取工艺参数（条件）为：温度48℃,料液比1∶19(g/mL),时间12 min,甲醇体积分数39%。结论：该提取方法稳定可靠,操作简便,可用于优化伏毛铁棒锤总生物碱提取工艺。比较不同地区伏毛铁棒锤中总生物碱含量,贵南县伏毛铁棒锤总生物碱含量最高,为0.290%。     

响应面法优化铁棒锤根中一种咪唑生物碱的超声提取工艺

采用响应面法优化铁棒锤根中一种咪唑生物碱Imidazole-2-Carboxylic acid butyl ester(ICABE)的超声提取工艺。在单因素试验基础上筛选出液料比、提取温度、提取时间三个主要因素,并通过响应面分析得到的最佳工艺条件为:液料比23 mL/g、提取温度58℃、提取时间51 min,在此条件下的理论提取率为0.081%,实际测得值为0.079%,两者较接近。验证试验表明,所得模型方程能较好地预测试验结果。     

响应面设计法优化仙鹤草总多酚的超声提取工艺

在单因素实验的基础上,采用响应面分析法对影响超声辅助提取仙鹤草总多酚得率的主要因素(超声温度、料液比和乙醇浓度)进行优化,建立了影响因素与总多酚之间的函数关系。获得最佳工艺条件为:71%乙醇,料液比1∶24,超声提取温度60℃,提取时间20 min。在此最佳条件下,总多酚得率为3.56%,试验结果与模型预测值相符。     

响应面法优化铁棒锤根中一种咪唑生物碱的超声提取工艺北大核心CSCD

采用响应面法优化铁棒锤根中一种咪唑生物碱Imidazole-2-Carboxylic acid butyl ester(ICABE)的超声提取工艺。在单因素试验基础上筛选出液料比、提取温度、提取时间三个主要因素,并通过响应面分析得到的最佳工艺条件为:液料比23 mL/g、提取温度58℃、提取时间51 min,在此条件下的理论提取率为0.081%,实际测得值为0.079%,两者较接近。验证试验表明,所得模型方程能较好地预测试验结果。     

响应面法优化超声提取苜蓿皂苷工艺条件

目的:利用响应面法对超声提取苜蓿皂苷的工艺条件进行了优化.方法:研究了超声波条件下影响提取的几个因素,包括超声时间、超声温度、超声功率、固液比等,并通过响应面法优化工艺条件.结论:根据中心组合设计原理采用四因子三水平的响应面分析法,通过对各因子显著性和交互作用的分析,得出了超声提取苜蓿皂苷的最佳工艺条件为:超声时间22min,超声温度43℃,超声功率403W,固液比1:51(g/ml),此时苜蓿皂苷的得率为4.53%.     

Box-Behnken响应面法优化余甘子总多酚超声提取工艺

以余甘子中总多酚的提取量为评价指标,通过单因素试验结合Box-Behnken响应面法,研究乙醇浓度、超声时间、超声温度对余甘子中总多酚提取工艺的影响,并优化提取工艺。结果表明：影响余甘子总多酚提取的因素主次为乙醇浓度>超声温度>超声时间,最佳提取工艺为乙醇浓度64%、超声时间39 min、提取温度43 ℃。验证试验中总多酚提取量为186.17 mg/g,与模型预测值190.33 mg/g较接近。Box-Behnken响应面回归方程拟合度良好,适合用于余甘子总多酚提取工艺的回归分析和参数优化。     

响应面法优化胡芦巴种子多糖提取工艺

利用单因素实验及响应面法优化确定胡芦巴种子多糖的提取工艺。通过单因素实验筛选出料液比、提取时间、提取温度三个主要因素,以胡芦巴种子多糖提取得率为响应值进行Box-Behnken中心组合试验设计,建立胡芦巴种子多糖提取得率的二次回归方程,得到优化组合条件。响应面法分析结果表明,当料液比为1∶28(g:mL),提取时间1.2 h,提取温度85℃时验证优化工艺得胡芦巴种子多糖最大提取得率19.89%,接近于模型预测值20.24%。     

响应面法优化超声提取苦瓜皂苷工艺条件的研究

利用响应面法对超声提取苦瓜皂苷的工艺条件进行了优化。在对超声时间、超声温度、超声功率、料液比等单因子实验的基础上,根据中心组合设计原理采用四因子三水平的响应面分析法,通过对各因子显著性和交互作用的分析,得出了超声提取苦瓜皂苷的最佳工艺条件为:超声时间41 min,超声温度62℃,超声功率402 w,料液比1:23,此时苦瓜皂苷的实际得率为3.03%。     

响应面法优化黑土豆色素提取工艺研究

以黑土豆为原料提取色素,在乙醇浓度、回流温度、回流时间和料液比四个单因素实验基础上,通过响应面法优化提取条件,利用 Box-Benhnken 设计模型,研究四个自变量之间的影响.结果表明,得到最佳提取工艺为回流温度47℃,回流时间47．5 min,料液比1∶90,乙醇浓度42．5％.在此条件下,理论上测得黑土豆中色素的吸光度值为0．413,实验测得黑土豆中色素的吸光度值为0．416,理论值与实验值的相对误差为0．726％.该提取工艺简便、具有较高的重现性和可行性.     

响应面法优化超声提取绿茶茶多酚工艺

利用响应面法对超声提取绿茶荼多酚的工艺条件进行优化,在单因素试验的基础上,根据中心组合设计原理采用三因素三水平的响应面分析法,依据回归分析确定最优提取工艺条件。结果表明,其最佳工艺条件为：液料比为40．2mL／g,超声功率为476W,提取时间为15．1min,采用该工艺条件,茶多酚的提取得率达到10．312％,通过响应面法得到一个能较好预测试验结果的模型方程。     

响应面法优化南方山荷叶中鬼臼毒素的提取工艺

鬼臼毒素（Podophyllotoxin）是一种常用的临床天然药物。本研究以鬼臼类药材南方山荷叶（Diphylleia sinensis H.L.Li）为材料,以提取物中鬼臼毒素的含量为控制指标,应用响应面法优化南方山荷叶中鬼臼毒素的提取工艺。采用高效液相色谱法测定提取物中鬼臼毒素的含量,在单因素实验筛选的基础上,选择出对鬼臼毒素提取率影响较大的3个因素：乙醇浓度、提取温度和提取时间,然后利用Design-expert软件设计3因素3水平响应面实验,最终获得鬼臼毒素提取率最高时的提取条件为：乙醇浓度48.18%、提取温度62.66℃、提取时间16.15 h,此时鬼臼毒素的理论提取率可达7.636%。进一步对该条件进行实验验证,3次实验平均值为7.54%,与理论值差异较小,表明该模型拟合良好,实验方法可靠。     

响应面法优化粉被虫草中虫草素的提取工艺

【背景】粉被虫草是民间常用药用真菌,具有抗辐射等多种药理作用,虫草素是其重要的活性物质。【目的】探究粉被虫草中提取虫草素的影响因素,为制取虫草素提供参考。【方法】以虫草素得率为指标,用响应面法优化超声提取工艺。分别考察超声时间、液料比和超声功率对提取效率的影响,在单因素实验基础上进行3因素3水平的响应面分析实验。【结果】最优提取条件为超声时间606 s、液料比45.9:1、超声功率400 W,在此条件下虫草素得率为6.136 mg/g,与模型预测值接近,方程拟合良好。【结论】首次对粉被虫草中虫草素的提取条件进行了研究,将对制取虫草素提供参考,有利于粉被虫草的深度开发利用。     

Optimization of extraction process of Glycyrrhiza glabra polysaccharides by response surface methodology

Experimental design was used to investigate the effect of three parameters (extraction time, extraction number and ratio of water to raw material) on polysaccharides yields. The ranges of the factors investigated were 3.5–4.5 h for extraction time (X₁), 4–6 for extraction number (X₂), and 25–35 for ratio of water to raw material (X₃). The statistical analysis of the experiment indicated that extraction time and ratio of water to raw material had significant effect on Glycyrrhiza glabra polysaccharides yields. The central composite design showed that polynomial regression models were in good agreement with the experimental results with the coefficients of determination of 0.924 for Glycyrrhiza glabra polysaccharides yield. The optimal condition for Glycyrrhiza glabra polysaccharides yield within the experimental range of the variables studied was at 4.3 h, 6, and 35. At this condition, the predicted yield of polysaccharides extracted was 3.6%.     

Optimization of ultrasonic-assisted extraction process of Poria cocos polysaccharides by response surface methodology

Polysaccharides production from Poria cocos was carried out using aqueous NaOH with the assistance of ultrasonic. Experimental design was used to investigate the effect of three parameters (extraction time, extraction concentration of NaOH, and ratio of aqueous NaOH to raw material) on polysaccharides yields. The ranges of the factors investigated were 1–3 min for extraction time (X₁), 0.5–1.0 mol/L for extraction concentration of NaOH (X₂), and 30–50 for ratio of aqueous NaOH to raw material (X₃). The statistical analysis of the experiment indicated that extraction concentration of NaOH had significant effect on P. cocos polysaccharides yields. The central composite design showed that polynomial regression models were in good agreement with the experimental results with the coefficients of determination of 0.9935 for P. cocos polysaccharides yield. The optimal condition for P. cocos polysaccharides yield within the experimental range of the variables studied was at 2.44 min, 0.789 mol/L, and 53.0. At this condition, the predicted yield of polysaccharides extracted was 82.3%.     

Optimization of ultrasonic-stimulated solvent extraction of sinigrin from Indian mustard seed (Brassica Juncea L.) using response surface methodology

Introduction – Sinigrin, a major glucosinolate present in Indian mustard (Brassica juncea L.) seeds as the precursor of the anticancer compound allyl isothiocyanate, shows a wide range of biological activities. It's necessary to optimize the extraction methods and conditions, in order to improve the extraction productivity and save raw material. Objective – To systemically investigate and optimize the most important factors affected the productivity of sinigrin in the process of extraction using response surface methodology. Methodology – The ranges of three main factors including the ethanol concentration, extraction time and extraction temperature were selected by the one‐factor‐at‐a‐time method. The conditions of ultrasonic‐stimulated extraction of sinigrin from defatted Indian mustard seed powder were optimized by Box‐Behnken design to obtain the maximum productivity. Result – The predicted productivity (3.81%) was obtained using 57% ethanol concentration at 81°C for 60 min, with the coefficient of the model R² > 0.96 (n = 17). The actual productivity (3.84 ± 0.02%) of sinigrin under the optimized condition was increased by 70.67% compared with the result of conventional extraction. Meanwhile, HPLC, UV and IR were applied to examine if there is a difference between the ultrasonic‐stimulated solvent extraction and conventional extraction, and the improvement of productivity of sinigrin depended on the destruction of cell wall caused by the elimination of outer pectinous material was explained by SEM and composition content analysis. Conclusion – The ultrasonic‐stimulated solvent extraction was suggested to be a promising method to improve the productivity of sinigrin. And the results demonstrated that sinigrin productivity may be related to pectinous materials existed in the seeds. Copyright © 2010 John Wiley & Sons, Ltd.     

Optimization of extraction process by response surface methodology and preliminary structural analysis of polysaccharides from defatted peanut (Arachis hypogaea) cakes

In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from defatted peanut cake. A central composite design including independent variables, such as extraction temperature (x₁), extraction time (x₂), and ethanol concentration (x₃) was used. Selected response which evaluates the extraction process was polysaccharide yield, and the second-order model obtained for polysaccharide yield revealed coefficient of determination of 97.81%. The independent variable with the largest effect on response was ethanol concentration (x₃). The optimum extraction conditions were found to be extraction temperature 48.7 °C, extraction time 1.52 h, and ethanol concentration of 61.9% (v/v), respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 25.89%. The results of structural analysis showed that the main composition of defatted peanut cake polysaccharide was α-galactose.     

Application of reverse micelle extraction process for amylase recovery using response surface methodology

The effect of different process variables of reverse micelle extraction process like pH, addition of surfactant (AOT) concentration and potassium chloride (KCl) concentration on amylase recovery has been studied and analysed. Solid-state fermentation was used for the production of amylase enzyme. Response surface methodology (RSM) using central composite rotatable design (CCRD) was employed to analyse and optimize the enzyme extraction process. The regression analysis indicates that the effect of AOT concentration, and KCl concentration were significant, whereas the effect of pH was non-significant on enzyme recovery. For the maximum recovery of enzyme, the optimum operating condition for pH, AOT concentration (M) and KCl concentration were 10.43, 0.05 and 1.00, respectively. Under these optimal conditions, the enzyme recovery was 83.16%.     

Optimization of polysaccharides (ABP) extraction from the fruiting bodies of Agaricus blazei Murill using response surface methodology (RSM)

Response surface methodoloty (RSM) was used to optimize the extraction conditions of polysaccharides (ABP) from the fruiting body of Agaricus blazei. A central composite design (CCD) was used for experimental design and analysis of the results to obtain the optimal processing parameters. Four independent variables such as extraction temperature (°C), ratio of water to raw material, number of extraction, and extraction time (h) were investigated. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. The 3-D response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. The optimum extraction conditions were as follows: extraction temperature 91 °C, ratio of water to raw material 14, number of extraction 6, and extraction time 2.1 h. Under these conditions, the experimental value was 65.8 ± 1.42, which is well in close agreement with value predicted by the model.