共查询到20条相似文献,搜索用时 15 毫秒
1.
Christopher A. Mitchell Miranda D. Grounds John M. Papadimitriou 《Cell and tissue research》1995,280(2):407-413
This study determined whether the genotype of bone marrow-derived inflammatory cells contributes to the more pronounced leukocytic exudation and extensive new muscle formation seen in SJL/J compared with BALB/c mice after a crush-injury (Mitchell et al. 1992). Female SJL/J mice were whole-body irradiated and reconstituted with male bone marrow from the BALB/c strain, and irradiated BALB/c females reconstituted with male SJL/J bone marrow. The mice were allowed to recover for 3 weeks and the tibialis anterior muscle (in a leg which had been protected from irradiation) was injured by crushing. At 3 and 10 days after injury the extent of necrotic debris, mononuclear leukocytic infiltration and new muscle formation was assessed in the muscles. The SJL/J mice reconstituted with BALB/c bone marrow showed extensive mononuclear leukocytic infiltration and clearance of necrotic debris when compared with BALB/c mice reconstituted with SJL/J bone marrow, and these strain-specific differences mirrored those seen with control bone marrow reconstituted hosts and non-irradiated hosts. The results show that the genotype of the bone marrow-derived macrophages is not responsible for the superior regeneration of crush-injured skeletal muscle in SJL/J mice, and it appears that factors intrinsic to the muscle tissue may be of central importance. 相似文献
2.
The patterns of skeletal muscle precursor cell replication after crush injury were compared by the use of autoradiographic techniques, in young (4-week-old) and old (39-week-old) BALBc and SJL/J mice. Similar comparisons were made between cut and crush lesions in old BALBc muscle. Muscle precursor cell replication commenced at 18–24 h after injury in both young and old muscles from both strains of mice. In young BALBc muscle the peak of myogenic activity at 60 h was 36 h earlier than in old mice. SJL/J muscle responded more rapidly than did BALBc: in young SJL/J the peak myogenic activity was at 46 h (14 h earlier than in young BALBc muscle), and in old SJL/J muscle the peak activity at 72 h was 24 h earlier than in old BALBc muscle. In all mice (both young and old) myogenic cell replication was substantially reduced by 120 h after injury. A comparison of the timing of muscle precursor cell replication in cut and crush lesions in old BALBc mice revealed a more rapid response in the cut lesion: this difference between the lesions in comparable with data from identical lesion in 6-8-week-old BALBc mice (McGeachie and Grounds 1987). However, the peak of myogenic replication in the older mice in the present study was some 26–36 h later than in the younger 6-8-week-old mice. These experiments show that, whilst muscle precursor cell replication commences at approximately the same time (about 24 h) after injury in young and old mice, the peak level of activity is delayed by some 24–36 h in old mice. In addition, the SJL/J mouse strain responds more rapidly and prolifically to muscle injury than does the BALBc strain. 相似文献
3.
Summary We test the proposal (McGeachie and Grounds 1985) that myogenesis following severe (crush) injury is prolonged compared with minor (cut) injury. Forty-four mice were injured with a cut and a crush lesion on different legs, and tritiated thymidine was injected at various times after injury (0 to 120 h), samples of regenerated muscle were taken 9d after injury and autoradiography was used to determine the initiation of muscle precursor replication, and duration of proliferation after the two different injuries.In both lesions replication of potential myoblasts was initiated 30 h after injury. Myogenesis was essentially completed in cut lesions by 96 h after injury, although the peak was finished by 60 h. In contrast, significant muscle precursor replication in crush lesions was still occurring 96 h after injury, and myogenesis was almost finished by 120 h. The pronounced difference in duration of myogenesis in different lesions strongly supports the original proposalThe extended duration of myogenesis in crush lesions, in conjunction with tritiated thymidine reutilisation, appears to account for conflicting experimental results in support of the concept of a circulating muscle precursor cell. 相似文献
4.
Summary Two different aspects of tritiated thymidine (3H-Tdr) reutilisation in skeletal muscle were examined. Injection of a high dose (7 Ci/g) of 3H-Tdr into mice prior to crush injury of skeletal muscle resulted in heavy labelling (grain counts) of myotube nuclei 9 d later. In contrast, myotube nuclei were essentially unlabelled when a low dose (1 Ci/g) of 3H-Tdr was injected at similar times with respect to injury. It was concluded that labelling seen after the high dose was due to reutilisation of 3H-Tdr. (Such 3H-Tdr reutilisation can account for the results of Sloper et al. (1970) which previously supported the concept of a circulating muscle precursor cell.) When replicating muscle precursors were labelled directly with 3H-Tdr 48 h after injury, the percentages of labelled myotube nuclei and the distribution of nuclear grain counts were similar with either high or low dose.We also investigated whether the light labelling seen in regenerated myotube nuclei after 9 d, when 3H-Tdr had been injected before the onset of myogenesis (as found by McGeachie and Grounds 1987), was due to 3H-Tdr reutilisation or, alternatively, to proliferation of local cells in the wound which subsequently gave rise to muscle precursors. Labelling of myotube nuclei was compared in mice injected with 3H-Tdr either 2 h before, or 2 h after injury. In another experiment, mice were injected 12 h after injury and lesions sampled 1, 12 or 36 h later, to see whether local cells were replicating 12 h after injury, and what labelled cells subsequently entered to wound. No difference was found in myotube labelling between mice injected before or after injury, and no cells replicating locally in the wound at 12 h after injury were observed. The results clearly show that the light labelling was due to 3H-Tdr reutilisation. 相似文献
5.
6.
Hwan-Wun Liu Shou-Terng Shang Chung-Faye Chao Charles Muller 《Cell and tissue research》1991,265(3):409-414
Summary A well-developed Golgi apparatus and rough and smooth endoplasmic reticulum in the principal cells of the mouse epididymis indicate active protein synthesis. Studies have shown that epididymal secretions are essential for sperm maturation. In a previous study, two wheat-germ agglutinin (WGA)-binding glycoproteins, GP-49 and GP-83, were identified on the surface of mature mouse sperm. In this study, synthesis and secretion of these two glycoproteins were investigated. Apparent WGA-binding was found on the stereocilia and in the apical region of principal cells in the corpus and cauda of epididymis. Post-fixation and pre-embedding cytochemical localization revealed that WGA-binding sites were situated in the Golgi apparatus, multivesicular bodies and stereocilia of principal cells. GP-49 and GP-83 were identified in the Nonidet P-40 homogenates of corpus and cauda epididymidis. In the epididymides of which ductuli efferentes had been ligated for more than 4 weeks, no sperm were found in the lumina of epididymal tubules. WGA-binding sites were present in the corpus and cauda; GP-49 and GP-83 were identified in tissue homogenates of the corpus and cauda as well. These findings suggest that GP-49 and GP-83 of mature sperm may be secreted by the principal cells of the corpus and cauda. These two molecules apparently conjugate to sperm whilst sperm transit through the epididymis. 相似文献
7.
Mikel H. Snow Ph.D. 《Cell and tissue research》1977,185(3):399-408
Summary Myosatellite cells were examined and quantified at the fine structural level of resolution during aging of skeletal muscles in mice and rats. Satellite cells in the soleus and gastrocnemius muscles of animals between eight and 30 months of age appeared, according to morphological criteria, metabolically less active than those examined in immature muscles. In the soleus muscle of the mouse, satellite cells decreased in number from 4.6% at eight months of age to 2.4% at 30 months. This decrease appeared to be due to the passage of some satellite cells into the interstitial space as a result of the formation of external lamina material around the entire satellite cell surface.This study was supported by NIH grant No. 5 S01-RR05356-13Appreciation is extended to Ms. Amy Erisman and Mr. Monroe Sprague for their excellent technical assistance on this project 相似文献
8.
Dr. John K. McGeachie 《Cell and tissue research》1989,257(2):455-457
Summary Cellular proliferation in skeletal muscle was measured throughout the first 4 weeks after denervation. Twenty four mice had one leg denervated, and 4 groups of 6 of these mice were injected with tritiated thymidine once daily for 7 days, either during the first, second, third or fourth week after denervation. Autoradiographic labelling of muscle and connective tissue nuclei in denervated muscles was compared with innervated muscles from the opposite innervated legs of the same mice. Labelling of connective tissue and muscle (myonuclear and satellite cell) nuclei was significantly higher in denervated muscles, compared with innervated muscles on the unoperated side. There were no significant differences among labelling of nuclei in muscles denervated for 1, 2, 3 or 4 weeks. However, connective tissue labelling after 1 week of denervation was significantly higher than at later times. This study shows that nuclei of muscle and connective tissue cells proliferate and turnover at high levels for at least one month after denervation. 相似文献
9.
Subpatent persistence of parasitemia was detected for up to 7 weeks after infection of BALB/c mice with Plasmodium yoelii. Serum taken from recovered mice maintained parasitemias in recipient mice at a subpatent level when transferred repeatedly at 2-day intervals. Single doses of serum from convalescent donors delayed the course of infection in recipients. Small doses of transferred hyperimmune serum had the same effect, whereas large doses (>0.5 ml) totally suppressed parasitemia. Only a single secondary challenge of recovered mice was required in order to produce a maximally protective hyperimmune serum. Mice completely protected from a primary challenge with P. yoelii by transfer of hyperimmune serum were not at all resistant to a second challenge given some weeks later. After transfer of hyperimmune serum into mice with established P. yoelii infection, parasitemia fell to subpatent levels within 48 hr. During the first 21 hr after serum transfer, a progressive reduction in the proportion of ring forms present in blood smears was observed. 相似文献
10.
Summary A mouse mammary tumor cell line, desingated JC, has been established from a spontaneously developed primary adenocarcinoma
of an aged virgin female BALB/c mouse. Isoenzyme analyses including glucose-6-phosphate dehydrogenase, lactate dehydrogenase,
and peptidase proved that this cell line is of murine origin and devoid of contamination from other species. Karyotyping revealed
that the number of chromosome ranged from 26 to 100, with a modal number of 40. Electron microscopic examination detected
the presence of tonofilament and desmosomes confirming its epithelial nature. In addition, no type B or C virus particle was
detected, although intracysternal A particle was observed occasionally. Tumorigenicity in immunocompetent syngeneic hosts
was easily established by s.c., i.p., and i.v. injection of viable JC tumor cells. A very weak immunogenicity of the JC tumor
was demonstrated through its immunization-challenging on syngeneic immunocompetent hosts. Although no rejection of JC tumor
was noted, a significant prolongation for the incubation period before an obvious and palpable tumor growth was detected between
the experimental and the control animals. Development of a concomitant immunity was also detected. The JC tumor represents
a valuable murine mammary tumor model which is different from other available models because of its unique origin, absence
of virus particles, very weak immunogenicity, and high tumorigenicity in syngeneic hosts. The cell line has been maintained
for more than 5 yr and has been used for experimental immunotherapy in our laboratory.
This work was supported by a research grant IM-416, awarded by the American Cancer Society, Atlanta, Georgia. 相似文献
11.
Summary We have recently shown that myogenesis following severe injury is prolonged compared with minor injury (McGeachie and Grounds 1987). In this previous autoradiographic study 44 mice were injected with tritiated thymidine at various times after muscle injury (0 to 120 h), and samples were taken 9d after injury to determine the percentage of labelled myotube nuclei. In the present study the same experimental data are analysed in detail to reveal how many times labelled muscle precursors divided before fusing to form myotubes.Additional mice were prepared and samples removed 1 h after injection of tritiated thymidine to determine the maximum grain counts of premitotic nuclei. When a labelled premitotic nucleus divides, each of the two daughter nuclei will contain half of the original label. The grain counts of nuclei resulting from sequential divisions of a maximally labelled premitotic nucleus, forms the basis for our detailed analysis which can reveal how many times a muscle precursor has divided after labelling.Nine days after injury the autoradiographic grain counts of labelled myotube nuclei were analysed in detail. The results describe an in vivo model of myogenesis which we use to evaluate quantitatively observations derived from tissue culture studies. The analysis shows that, at the onset of myogenesis in regenerating muscle (30 h after injury), muscle precursors divide only twice before fusing to form myotubes. This observation challenges the concept of quantal mitosis as defined by the tissue culture studies of Quinn et al. (1984, 1985). 相似文献
12.
Hai LI Hu REN Lei CAO Jinyuan GUO Jingjing SONG Yan ZHANG Wenbo XU 《Experimental Animals》2022,71(4):500
Human respiratory syncytial virus (HRSV) is a major cause of lower respiratory tract infection in infants. The lack of ideal animal models is one of the major obstacles in evaluateing the efficacy of HRSV vaccines. In this study, HRSV-50 was obtained from Hep-2 cells at the 50th passage of the original Long strain (ATCC VR-26). BALB/c mice (6 weeks) were challenged with different titers of HRSV-50. Shockingly, all mice died after 4 days of challenge (6 × 106 PFU/mouse). Whole-genome sequencing revealed 7 amino acid mutations compared with the original Long strain. To verify whether the lethal model can be used to effectively evaluate the efficacy of HRSV candidate vaccines, we studied the protective effect of FRBD protein (Pre-F of HRSV and S receptor binding domain of SARS-CoV-2) with Adju-phos or MA103 adjuvant. All mice in the PBS group died after the HRSV-50 challenge, whereas Adju-phos provided partial protection. These results suggest that we have successfully established a lethal model of HRSV in BALB/c mice. 相似文献
13.
目的分析SPF级封闭群KM小鼠及近交系BALB/c小鼠的肠道菌群总菌多样性,比较两个不同遗传背景肠道总菌的丰富度、Shannon-Wiener指数和均匀度。方法收集SPF级KM小鼠和BALB/c小鼠新鲜粪便,提取粪便总菌DNA,用基于细菌16S rDNA序列的变性梯度凝胶电泳(PCR-DGGE)分析粪便总菌多样性。结果 SPF级KM小鼠及BALB/c小鼠粪便总菌多样性差异无统计学意义(P0.05),品系内不同性别之间粪便总菌多样性差异亦无统计学意义(P0.05)。结论选择SPF级小鼠进行微生态学相关研究时,封闭群KM小鼠及近交系BALB/c小鼠均可作为选择对象,同时可忽略菌群的性别差异。 相似文献
14.
Hwan-Wun Liu Guang-Huan Sun Shyi-Ren Shy Hann-Yeh Shyu 《Cell and tissue research》1992,269(2):189-194
Summary The secretion of sperm maturation-related molecules by the epididymis is subjected to developmental and hormonal regulation. In the BALB/c mouse, we found that GP-83 and GP-49, two sperm maturationrelated glycoproteins, were secreted by the epididymis. The present study investigated the postnatal development and testosterone-dependence of these two molecules. Histochemical localization in paraffin sections revealed that wheat-germ agglutinin (WGA)-binding sites were first present in the epididymis of 4-week-old mice. The distribution of WGA-binding sites was the same as that of more mature mice, i.e., it was first found in the principal cells of the corpus epididymidis, and gradually appeared in the contents of epididymal tubules. On WGA blots, GP-83 and GP-49 were identified in the corpus, and GP-83 was identified in the cauda of the epididymis. In mice that had received unilateral orchiectomy at 4 weeks of age, GP-83 and GP-49 were present in both intact and orchiectomized epididymides 4 weeks after the operation. In the epididymides of mice that had received bilateral orchiectomy, GP-83 and GP-49 were barely identifiable. However, the presence of these two molecules was restored if testosterone was supplemented immediately after orchiectomy. These results indicate that GP-83 and GP-49 are secreted de novo in the epididymis, and that the secretion of these two molecules is developmentally regulated and androgen-dependent. 相似文献
15.
Expression of caveolin-1 and -3 in mouse smooth muscle cells in vivo was examined by immunohistochemistry. Caveolin-1 was detected in almost all smooth muscles examined, except for the pupillary dilator muscle, whereas caveolin-3 was present only in smooth muscles of some specific tissues. In the eye, the pupillary sphincter muscle was intensely positive for caveolin-3, whereas the ciliary muscle and pupillary dilator muscle were negative. In the gastrointestinal tract, caveolin-3 was detected in the inner circular layer, but not in the outer longitudinal layer. Vascular smooth muscle cells of the resistance-sized artery in the uterus and corpus cavernosum were intensely positive for caveolin-3, whereas those of the aorta were only weakly positive and those of the vena cava were negative. Caveolin-3 was also detected in smooth muscle cells of the urinary bladder, ureter, prostatic vas deferens, and seminal vesicle. The different levels of caveolin-3 expression among various smooth muscle tissues were confirmed by Western blot analysis. Even within the same muscle, the relative expression levels of caveolin-1 and -3 were variable among neighboring cells, suggesting distinct fine regulation of expression of these two caveolins. Moreover, even in the same cell, caveolin-1 and -3 showed different distributions. These results indicate that the two caveolins form distinct caveolae in smooth muscles, and that caveolin-1 and -3 serve different functions. Their differential expression may therefore be related to the functional diversity of smooth muscles. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of the Japanese Government. 相似文献
16.
【背景】膳食纤维被认为是第七类营养素,主要在单胃动物后肠被微生物利用。【目的】研究典型可溶性膳食纤维燕麦β-葡聚糖和典型不可溶性膳食纤维微晶纤维素(MCC)对小鼠结肠细菌群落结构和组成的影响。研究结果可为动物含纤维饲粮的科学配制提供参考,并为人类食品中不同类型膳食纤维的合理利用提供一定借鉴。【方法】选用27只6周龄健康雄性BALB/c小鼠(18.13±0.95 g),按体重无差异原则随机分为3组,分别饲喂含20%MCC(纯度≥99%,M组),28%燕麦β-葡聚糖(纯度为70%,G组)和不含膳食纤维(对照组)的饲粮,试验期为21 d。试验结束后每个处理随机选取3只小鼠处死,收集结肠食糜,利用PCR-DGGE(Polymerase chain reaction-denaturing gradient gel electrophoresis)和高通量测序技术比较分析各组小鼠结肠食糜细菌群落结构的差异。【结果】3组小鼠结肠细菌PCR-DGGE图谱条带丰富度和Shannon指数存在明显差异,表现为G组低于M组和对照组(P=0.027,0.035);聚类分析发现,3组小鼠各有2个样品聚于一簇,各组条带相似性为:G组71%,M组55%,对照组67%。高通量测序发现,3组小鼠结肠细菌Shannon指数和β-多样性指数存在显著差异(P=0.047,0.035);Bacteroidetes、Firmicutes和Proteobacteria为小鼠结肠中的优势细菌门类,占总比例的95.9%-99.4%。与对照组相比,G组小鼠结肠Bacteroidetes相对丰度升高26.78%,M组降低15.62%,其中S27_4科属水平未分类细菌和Bacteroides属细菌对这种差异的贡献最大(P=0.099,0.051);G组Firmicutes相对丰度较对照组降低28.99%,而M组比对照组高15.82%,且该差异主要由Clostridiales目某属细菌、Ruminococcaceae科某属细菌和Lactobacillus属细菌造成(P=0.027、0.061和0.079)。【结论】两种类型的膳食纤维均对小鼠结肠细菌群落结构产生影响,饲粮中添加高水平燕麦β-葡聚糖降低了小鼠结肠细菌群落的多样性;小鼠结肠存在特异性利用两种纤维的菌群;S27_4科细菌更偏好于利用燕麦β-葡聚糖等植物性多糖,Clostridiales目可能存在特异性利用纤维素的细菌种群。 相似文献
17.
Summary Disc formation of rod photoreceptor cells in developing BALB/c mice retinas was studied by rapid freeze, freeze-substitution, freeze-etching, immunocytochemistry, and myosin S-1 decoration methods. Freeze-substituted photoreceptor cells contained variously shaped vesicles in the apical swelling of the connecting cilium or the base of the outer segment during postnatal development. Rapid freezing successfully arrested pinocytosis; the fusion of small vesicles to give large ones, and the compression of certain vesicles (0.3–0.6 m) appears to lead gradually to the formation of the so-called discs. We therefore propose that membranous discs are formed by the fusion of small pinocytotic vesicles and their subsequent compression. Discs formed in this way were partially stacked, but were ordered at random during the early developmental stages. During development, a partial stack of discs was progressively rearranged to a regular form as seen in mature outer segments. Cytoskeletal actin was expected to be involved in the disc formation; it was demonstrated in the distal axoneme of the connecting cilium during development and showed no change in its distribution. However, the polarity of the actin filaments, as revealed by myosin S-1 decoration in early developmental stages, was much more variable than in the adult. Barbed ends of actin filaments were associated with the plasma membrane or the membrane of vesicles. We also found actin filaments coiled up helically on ciliary microtubules. 相似文献
18.
The kinetics of primary and secondary infections with Taenia crassiceps larvae and the effects of immune serum on T. crassiceps larvae were studied in BALB/c and BDF1 mice. In both strains of mice a substantial degree of resistance to reinfection comparable to that previously reported in C3H mice can be induced by subcutaneous injection of three larvae 3 weeks prior to intraperitoneal challenge infection. Both early immune damage in the absence of adherent host cells and encapsulation by host cells are involved in rejection of larvae by BALB/c and BDF1 mice, but in both of these strains early immune damage is less pronounced and the cellular encapsulation response considerably more prominent than in the C3H mice studied previously. This difference is also reflected in the effect of immune serum on T. crassiceps metacestodes in vitro: immune serum from BALB/c and BDF1 mice is less effective than immune serum taken from C3H mice at comparable times after challenge infection in mediating damage to T. crassiceps larvae in vitro in the absence of host cells. These results suggest that genetically determined differences in immune capability can alter the state of equilibrium existing among different immune effector mechanisms without producing measurable effects upon overall host resistance to reinfection. 相似文献
19.
BALB/c小鼠金属硫蛋白-1 cDNA基因在乳酸菌质粒表达载体pNZ2103上的克隆 总被引:1,自引:0,他引:1
目的用乳酸菌质粒表达载体pNZ2103克隆BALB/c小鼠金属硫蛋白(MT)-1 cDNA基因。方法设计一对含有限制酶Pst1和HindⅢ位点的引物。以质粒pET21a-MT为模板,采用PCR法扩增BALB/c小鼠MT-1cDNA。用限制性内切酶Pst1和HindⅢ将MT-1 cDNA克隆于质粒pNZ2103形成重组质粒pNZ2103-MT。将pN2103-MT转化进入大肠埃希菌DH5α。采用PCR法和Pat1、HindⅢ双酶切方法鉴定含有pNZ2103-MT的转化子。12% SDS-PAGE鉴定pNZ2103-MT在大肠埃希菌DH5α的表达水平。结果获得含有pNZ2103-MT的DH5α转化子。结论以乳酸菌质粒表达载体pNZ2103构建的pNZ2103-MT在大肠埃希菌中表达水平较低,采用SDS-PAGE难以检测到表达水平的金属硫蛋白。 相似文献
20.
Faussone-Pellegrini MS Vannucchi MG Ledder O Huang TY Hanani M 《Cell and tissue research》2006,325(2):211-217
Ablation of the myenteric plexus in mouse colon with the detergent benzalkonium chloride (BAC) is followed by considerable recovery of the nerves, indicating that this plexus is capable of regeneration and has plasticity. Interstitial cells of Cajal (ICC) are closely associated with enteric nerves, and the acquisition and maintenance of their adult phenotype are nerve-dependent. Little is known about the regenerative processes of ICC or about the possible dependence of these processes on neurons. To address these questions, we ablated the myenteric plexus in the mouse colon with BAC and followed changes in the adjacent ICC (ICC-MP) from day 2 to day 70 after treatment, by using c-kit-immunohistochemistry and electron microscopy. In the untreated area, c-kit-positive cells and ICC-MP with normal ultrastructural features were always present. The region partially affected by BAC contained some c-kit-positive cells, and either normal or vacuolated ICC-MP were observed by electron microscopy. Moreover, at days 60–70, ICC-MP with particularly extended rough endoplasmic reticulum were present in this area. In the treated area, either denervated or reinnervated, c-kit-positive cells were always absent. By day 14 after BAC treatment, nerve fibers had started to grow back into the treated region and, in the reinnervated area, cells with fibroblast-like features appeared and were seen to contact both nerve endings and smooth muscle cells and to acquire some typical ICC features. Thus, ICC are vulnerable to external insult but appear to have some ability to regenerate.This work was supported by the US-Israel Binational Science Foundation (BSF, 98-00185; to M.H.) and University funds “quota di ateneo ex 60%” (M.-S. F.-P.). 相似文献