Sequence stratigraphy of the Early–Middle Devonian succession (Padeha Formation) in Tabas Block,East-Central Iran: Implication for mixed tidal flat deposits

The facies and sequence stratigraphic analyses of the Padeha Formation (Early–Middle Devonian) in the East-Central Iran are based on two measured stratigraphic sections in the Ozbak-Kuh (type section) and Deranjal (Dahaneh-Kalot section) Mountains in the northern Tabas Block, with a thickness of 492 and 320 m respectively. The field observations and laboratory studies were used to identify sixteen lithofacies and four architectural elements. The facies associations were classified into five categories including sandstone (St, Sp, Sr, Sh, Sl), fine grained (siltstone–claystone) or mud rock (Fl), interbedded sandstone–mud rock (Sr(Fl), Sr/Fl, Fl(Sr)), dolomite (Dl and Ds), and evaporate (El, Efl, Efm, Edl). Structural (wave and interference ripples, planar cross-bedding and herringbone, flaser-wavy and lenticular beddings, tepee and stromatolite structures, entrolothic foldings, shrinkage and syneresis cracks, rain drop imprints, and salt casts) and textural (mature–supermature quartzarenite) features, as well as predominant bimodal pattern of paleocurrents in most lithofacies, show that sediments of the Padeha Formation have been deposited in a tidal flat environment. The sandstone, interbedded sandstone–mud rock, and dolomite facies (SB and LA architectural elements) are related to subtidal and intertidal settings, and fine-grained sediments and evaporate facies (consisting of FF and E architectural elements) were deposited in the supratidal and sabkha settings. Detail sequence stratigraphic study led to identification of two 3rd order complete depositional sequences (DS₂ and DS₃) with SB₁ sequence boundaries and two incomplete sequences (DS₁ and DS₄). Supratidal and sabkha facies were formed during the fall of relative sea level and are related to the lowstand systems tract (LST). The intertidal facies were formed at a rise of relative sea level and are interpreted as transgressive (TST) and highstand (HST) systems tracts.     

Quantitative calcareous nannofossil biostratigraphy of the subsurface Pliocene sequence,north-east Nile Delta,Egypt

Detailed quantitative calcareous nannofossil biostratigraphy was carried out on a subsurface lower–upper Pliocene succession (Kafr El Sheikh Formation) in three offshore wells (Ras El Barr-1, Bougaz-1 and Bougaz East-1), north-east Nile Delta area, Egypt. The standard zonations are easily applied in these sediments and additional biohorizons have been adopted in this study. For early to late Pliocene interval, the following five bioevents are considered reliable: last occurrences (LOs) of Discoaster surculus, D. tamalis, Reticulofenestra pseudoumbilicus and Amaurolithus tricorniculatus and first occurrence of D. asymmetricus. The LO of R. pseudoumbilicus was used to detect the early/late Pliocene boundary in the three studied wells. In Bougaz-1-Well, the LO of D. intercalcaris was used to approximate the Miocene/Pliocene boundary. The calcareous nannofossils are moderate to good preserved. Lower–upper Pliocene sediments are dominated by small-sized reticulofenestrids (R. minuta), Pseudoemiliania lacunosa, Helicosphaera carteri and H. kamptneri. Large size (>7 μm) R. pseudoumbilicus is recorded. Discoaster species is rarely represented. No nannofossils were found in the top part of the Kafr El Sheikh and the overlying Wastani Formations.     

Sequence stratigraphic analysis and sea-level history of the Upper Jurassic deposits (Mozduran Formation), south of Aghdarband,NE Iran

Abstract

The Kopet-Dagh Basin is a large sedimentary basin in northeastern Iran that host the giant Khangiran and Gonbadli gas fields. The Mozduran Formation with its various sedimentary facies is an important reservoir widely distributed in the basin. A sedimentological analysis of Upper Jurassic Mozduran Formation resulted in an accurate reconstruction of the sedimentary environments and the sequence stratigraphic framework south of Aghdarband. The strata consist of six different facies associations including 12 carbonate, one evaporate and two siliciclastic subfacies. On the basis of their various components, structural and textural characteristics, these facies were deposited on a homoclinal ramp in tidal flat to open marine environments ranging from supratidal to subtidal settings. Facies A1 and A2 represent open marine, B1-B4 Shoal, C1-C4 lagoonal and D1, D2, E, T1 and T2 tidal flat and Salina environments. In addition, based on detailed field and laboratory studies on the facies architecture, several large-scale (long-term) depositional sequences could be distinguished in the stratigraphic sections of the study area. These sequences are composed of LST, TST and HST that are separated by a SB1 and SB2 sequence boundaries. The paleogeography of the study area during the Late Jurassic time is reconstructed in five block diagrams.     

Stratigraphy and microfacies of the oligocene sequence at Gabal Bu Husah, Marada Oasis, South Sirte Basin, Libya

Summary Stratigraphic and microfacies investigations carried out on the Oligocene sequence exposed at Gabal Bu Husah, northwest of Marada Oasis, south of the Sirte Basin, Libya, showed that the Oligocene sequence conformably overlies the Late Eocene Thamat Formation and unconformably underlies the Early to Middle Miocene Marada Formation (Qarat Jahannam Member). The lithostratigraphic studies of the Oligocene sequence revealed the presence of two rock units, from base to top: 1- Umm Ad Dahiy Formation (Early Oligocene, Rupelian) and 2- Bu Hashish Formation (Late Oligocene, Chattian). The Oligocene sequence yields a rich foraminiferal assemblage with fifty-one benthonic and large formaminiferal species. The foraminiferal analysis allowed to subdivide the sequence into three local foraminiferal assemblage zones, arranged stratigraphically from base to top: 1)Elphidium minutum zone comprising the Umm Ad Dahiy Formation. 2)Miogypsinoides complanatus/Nonion granosus assemblage zone covering the lower two thirds of the Bu Hashish Formation. 3) Zone with abundantNummulites spp., including the upper part of the Bu Hashish Formation. The paleoenvironmental significance of the recorded species is described and discussed. The microfacies studies led to the recognition of eleven microfacies types. These microfacies indicate that the lower part of the Umm Ad Dahiy Formation was deposited in a shallow warm marine environment, but the conditions changed to fluviomarine in the uppermost part. The Bu Hashish Formation was deposited in a shallow-marine, inner-shelf environment (as indicated by the operculinid limestone) but a probable hiatus in its middle part is indicated by the presence of a nummulitic coquina and gypsum beds formed in a lagoonal environment. After returning to a shallow marine environment at the end of Oligocene the marine sedimentation ended.     

Les kystes de dinoflagellés du Crétacé moyen de la marge atlantique brésilienne, le bassin de Santos

Organic dinoflagellate cysts are studied from the sedimentary sequence of hole 1-SPS-14A, drilled during oil exploration in the Santos Basin, Brazilian continental margin. The Ariri and Florianópolis Formations (Transitional sequence) do not contain any dinocysts. The oldest found dinocysts occur at the base of the Drift sequence in sediments, within platform carbonates of the Guarujá Formation. Continuous sea-level rise throughout the late Albian and Cenomanian submerged the carbonate platform with the terrigenous input of the Itanhaém Formation. The transgressive phase reached its peak during the Cenomanian/Turonian transition. Pelites were deposited during oceanic anoxic event (OAE-2), consisting the lower part of the Itajaí-Açu Formation. Normal oceanic conditions re-established in the late Turonian. The Brazilian dinocyst assemblage has tethyan affinities. Some species (i.e., Dinopterygium cladoides, Litosphaeridium arundum, Odontochitina rhakodes and Systematophora cretacea) suggest a middle Albian age for the carbonate platform of the Guarujá Formation. The lower part of the Guarujá Formation was not dated by other microfossils. An uppermost Albian or lower Cenomanian age is suggested for the base of the Itanhaém Formation on the basis of species Palaeohystrichophora infusorioides and Ovoidinium verrucosum. The Cenomanian-Turonian boundary cannot be characterized by dinocysts. Species Atopodinium iuvene, which is known from Turonian sediments in Europe, was found at the top of the Itajaí-Açu Formation. The observed dinocyst bioevents (i.e., last occurrence) are correlated with known foraminiferal, nannofossil and other palynological bioevents. The diversity of the assemblages remains constant throughout the various palaeoenvironments as these are reflected by the Guarujá and Itajaí-Açu Formations, but relative abundances of taxa are variable. Genera Coronifera, Florentinia, Ovoidinium, Spiniferites and Trichodinium are abundant in the carbonate platform assemblages (Guarujá Formation). Genera Cribroperidinium and Cyclonephelium are abundant in detrital sediments (Itajaí-Açu Formation). Only one species (Subtilisphaera guarujaensis n. sp.) is restricted to the carbonate platform environment.     

Molecular Insights Into the Evolutionary Pathway of Vibrio cholerae O1 Atypical El Tor Variants

Pandemic V. cholerae strains in the O1 serogroup have 2 biotypes: classical and El Tor. The classical biotype strains of the sixth pandemic, which encode the classical type cholera toxin (CT), have been replaced by El Tor biotype strains of the seventh pandemic. The prototype El Tor strains that produce biotype-specific cholera toxin are being replaced by atypical El Tor variants that harbor classical cholera toxin. Atypical El Tor strains are categorized into 2 groups, Wave 2 and Wave 3 strains, based on genomic variations and the CTX phage that they harbor. Whole-genome analysis of V. cholerae strains in the seventh cholera pandemic has demonstrated gradual changes in the genome of prototype and atypical El Tor strains, indicating that atypical strains arose from the prototype strains by replacing the CTX phages. We examined the molecular mechanisms that effected the emergence of El Tor strains with classical cholera toxin-carrying phage. We isolated an intermediary V. cholerae strain that carried two different CTX phages that encode El Tor and classical cholera toxin, respectively. We show here that the intermediary strain can be converted into various Wave 2 strains and can act as the source of the novel mosaic CTX phages. These results imply that the Wave 2 and Wave 3 strains may have been generated from such intermediary strains in nature. Prototype El Tor strains can become Wave 3 strains by excision of CTX-1 and re-equipping with the new CTX phages. Our data suggest that inter-chromosomal recombination between 2 types of CTX phages is possible when a host bacterial cell is infected by multiple CTX phages. Our study also provides molecular insights into population changes in V. cholerae in the absence of significant changes to the genome but by replacement of the CTX prophage that they harbor.     

An aberrant hemolysin of Vibrio cholerae non-O1.

An aberrant hemolysin produced by a Vibrio cholerae non-O1 strain N037 (N037-hly) was purified and characterized. N037-Hly was antigenically very similar to El Tor hemolysin but differed in molecular weight (48,000 vs. 60,000), interaction with glucose, and hemolytic activity. Of 100 V. cholerae non-O1 strains other than the N037 strain examined, none produced this aberrant hemolysin. The N-terminal amino acid sequence of N037-hly was highly homologous to that of El Tor hemolysin.     

Protective Effects of Gallium, Germanium, and Strontium Against Ovariectomized Osteoporosis in Rats

The effects of trace elements of gallium (Ga), germanium (Ge), and strontium (Sr) on ovariectomized (OVX) osteopenic rats were studied in this paper. The urine calcium content, serum calcium, and phosphorus contents, bone mineral content, mineral dissolution, and mechanical strength of the osteopenic rats were analyzed respectively. After the rats were fed with Ga, Ge, and Sr diet for 8 weeks, respectively, the urine calcium content decreased (P?<?0.01). Plasma calcium and phosphate concentrations decreased in the order of OVX group?>?Ge group?>?Sr group?>?Ga group?>?Sham group. Mineral content increased in the order of OVX group?<?Ge group?<?Sr group?<?Ga group?<?Sham group. A dramatic decrease in calcium solubility was found both in the gallium and strontium treated animals (P?<?0.05). However, the same result did not occur in germanium treated groups. The data provide an important proof of concept that gallium and strontium might be a new potential therapy for the management of postmenopausal osteoporosis in humans.     

Purification of El Tor cholera enterotoxins and comparisons with classical toxin.

In 55 clinical isolates of Vibrio cholerae biotype El Tor, cholera toxin (CT) production was higher after growth in liquid medium first under relatively anaerobic conditions followed by excessive aeration (AKI conditions) as compared with growth under the optimal conditions for CT production from V. cholerae of classical biotype (median toxin level being 400 ng ml-1 and 1 ng ml-1 respectively, for the two different growth conditions). Large growth volumes further enhanced El Tor toxin production to levels at or above 3-5 micrograms ml-1 from several strains, which allowed for easy purification of toxin by salt precipitation, aluminium hydroxide adsorption and/or GM1 ganglioside affinity chromatography. However, such purified El Tor CT completely lacked the A subunit when examined by SDS-PAGE or by monoclonal anti-A subunit antibody GM1-ELISA. In contrast, when El Tor CT was prepared from bacteria grown in the presence of specific antiserum against soluble haemagglutinin/protease it contained the A subunit (unnicked) in the same proportion to the B subunit (1A:5B) as classical CT. Immunodiffusion-in-gel tests revealed that the B subunits of El Tor and classical CTs share major epitopes but also have one or more weaker biotype-specific epitopes. The two types of toxin were practically indistinguishable in various GM1-ELISA tests, and antisera raised against El Tor and classical CT, respectively, could also completely neutralize the heterologous as well as the homologous toxin activity in vivo. The results indicate that CTs from El Tor and classical V. cholerae, despite demonstrable epitope differences, are predominantly cross-reactive and give rise to antisera with strong cross-neutralizing activity.     

PCR-based identification of Vibrio cholerae and the closely related species Vibrio mimicus using the large chromosomal ori sequence of Vibrio cholerae

The bacterial chromosomal replication origin (ori) sequences are a highly conserved essential genetic element. In this study, the large chromosomal replication origin sequence of Vibrio cholerae (oriCIVC) has been targeted for identification of the organism, including the biotypes of serogroup O1. The oriCIVC sequence-based PCR assay specifically amplified an 890 bp fragment from all the V. cholerae strains examined. A point mutation in the oriCIVC sequence of the classical biotype of O1 serogroup led to the loss of a BglII site, which was utilized for differentiation from El Tor vibrios. Interestingly, the PCR assay amplified a similarly sized ori segment, designated as oriCIVM, from V. mimicus strains, but failed to produce any amplicon with other strains. Cloning and sequencing of the oriCIVM revealed high sequence similarity (96%) with oriCIVC. The results indicate that V. mimicus is indeed very closely related to V. cholerae. In addition, the BglII restriction fragment length polymorphism (RFLP) between oriCIVM and oriCIVC sequences allowed us to differentiate the two species. The ori sequence-based PCR-RFLP assay developed in this study appears to be a useful method for rapid identification and differentiation of V. cholerae and V. mimicus strains, as well as for the delineation of classical and El Tor biotypes of V. cholerae O1.     

Genetic lesions within the 3a gene of SARS-CoV

Phage N5 is one of the phages of Vibrio cholerae serovar O1 biotype El Tor (Ghosh, A. N., Ansari, M. Q., and Dutta, G. C. Isolation and morphological characterization of El Tor cholera phages. J. Gen. Virol. 70: 2241–2243, 1989). In the present communication the growth curve, molecular weight and confirmation of the genome, partial denaturation map and restriction endonuclease digestion pattern have been determined. Partial denaturation map indicates that the genome has non-permuted / invariant sequence. Presence of cohesive ends has also been documented.     

Genome Sequence of Hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and Comparative Genomics with V. cholerae

The genomes of Vibrio cholerae O1 Matlab variant MJ-1236, Mozambique O1 El Tor variant B33, and altered O1 El Tor CIRS101 were sequenced. All three strains were found to belong to the phylocore group 1 clade of V. cholerae, which includes the 7th-pandemic O1 El Tor and serogroup O139 isolates, despite displaying certain characteristics of the classical biotype. All three strains were found to harbor a hybrid variant of CTXΦ and an integrative conjugative element (ICE), leading to their establishment as successful clinical clones and the displacement of prototypical O1 El Tor. The absence of strain- and group-specific genomic islands, some of which appear to be prophages and phage-like elements, seems to be the most likely factor in the recent establishment of dominance of V. cholerae CIRS101 over the other two hybrid strains.Vibrio cholerae, a bacterium autochthonous to the aquatic environment, is the causative agent of cholera, a life-threatening disease that causes severe, watery diarrhea. Cholera bacteria are serogrouped based on their somatic O antigens, with more than 200 serogroups identified to date (6). Only toxigenic strains of serogroups O1 and O139 have been identified as agents of cholera epidemics and pandemics; serogroups other than O1 and O139 have the potential to cause mild gastroenteritis or, rarely, local outbreaks. Genes coding for cholera toxin (CTX), ctxAB, and other virulence factors have been shown to reside in bacteriophages and various mobile genetic elements. In addition, V. cholerae serogroup O1 is differentiated into two biotypes, classical and El Tor, by a combination of biochemical traits, by sensitivity to biotype-specific bacteriophages, and more recently by nucleotide sequencing of specific genes and by molecular typing (5, 17, 19).There have been seven pandemics of cholera recorded throughout human history. The seventh and current pandemic began in 1961 in the Indonesian island of Sulawesi and subsequently spread to Asia, Africa, and Latin America; the six previous pandemics are believed to have originated in the Indian subcontinent. Isolates of the sixth pandemic were almost exclusively of the O1 classical biotype, whereas the current (seventh) pandemic is dominated by the V. cholerae O1 El Tor biotype as the causative agent, a transition occurring between 1923 and 1961. Today, the disease continues to remain a scourge in developing countries, confounded by the fact that V. cholerae is native to estuaries and river systems throughout the world (8).Over the past 20 years, several new epidemic lineages of V. cholerae O1 El Tor have emerged (or reemerged). For example, in 1992, a new serogroup, namely, O139 of V. cholerae, was identified as the cause of epidemic cholera in India and Bangladesh (25). The initial concern was that a new pandemic was beginning; however, the geographic range of V. cholerae O139 is currently restricted to Asia. Additionally, V. cholerae O1 hybrids and altered El Tor variants have been isolated repeatedly in Bangladesh (Matlab) (23, 24) and Mozambique (1). Altered V. cholerae O1 El Tor isolates produce cholera toxin of the classical biotype but can be biotyped as El Tor by conventional phenotypic assays, whereas V. cholerae O1 hybrid variants cannot be biotyped based on phenotypic tests and can produce cholera toxin of either biotype. These new variants have subsequently replaced the prototype seventh-pandemic V. cholerae O1 El Tor strains in Asia and Africa, with respect to frequency of isolation from clinical cases of cholera (27).Here, we report the genome sequence of three V. cholerae O1 variants, MJ-1236, a Matlab type I hybrid variant from Bangladesh that cannot be biotyped by conventional methods, CIRS101, an altered O1 El Tor isolate from Bangladesh which harbors ctxB of classical origin, and B33, an altered O1 El Tor isolate from Mozambique which harbors classical CTXΦ, and we compare their genomes with prototype El Tor and classical genomes. From an epidemiological viewpoint, among the three variants characterized in this study, V. cholerae CIRS101 is currently the most “successful” in that strains belonging to this type have virtually replaced the prototype El Tor in Asia and many parts of Africa, notably East Africa. This study, therefore, gives us a unique opportunity to understand why V. cholerae CIRS101 is currently the most successful El Tor variant.     

Whole genome PCR scanning reveals the syntenic genome structure of toxigenic Vibrio cholerae strains in the O1/O139 population

Vibrio cholerae is commonly found in estuarine water systems. Toxigenic O1 and O139 V. cholerae strains have caused cholera epidemics and pandemics, whereas the nontoxigenic strains within these serogroups only occasionally lead to disease. To understand the differences in the genome and clonality between the toxigenic and nontoxigenic strains of V. cholerae serogroups O1 and O139, we employed a whole genome PCR scanning (WGPScanning) method, an rrn operon-mediated fragment rearrangement analysis and comparative genomic hybridization (CGH) to analyze the genome structure of different strains. WGPScanning in conjunction with CGH revealed that the genomic contents of the toxigenic strains were conservative, except for a few indels located mainly in mobile elements. Minor nucleotide variation in orthologous genes appeared to be the major difference between the toxigenic strains. rrn operon-mediated rearrangements were infrequent in El Tor toxigenic strains tested using I-CeuI digested pulsed-field gel electrophoresis (PFGE) analysis and PCR analysis based on flanking sequence of rrn operons. Using these methods, we found that the genomic structures of toxigenic El Tor and O139 strains were syntenic. The nontoxigenic strains exhibited more extensive sequence variations, but toxin coregulated pilus positive (TCP+) strains had a similar structure. TCP+ nontoxigenic strains could be subdivided into multiple lineages according to the TCP type, suggesting the existence of complex intermediates in the evolution of toxigenic strains. The data indicate that toxigenic O1 El Tor and O139 strains were derived from a single lineage of intermediates from complex clones in the environment. The nontoxigenic strains with non-El Tor type TCP may yet evolve into new epidemic clones after attaining toxigenic attributes.     

EARLY DEVONIAN FISHES FROM GUIJIATUN AND XUJIACHONG FORMATIONS OF QUJING,YUNNAN,AND RELATED BIOSTRATIGRAPHIC PROBLEMS

Pale0nt0IogicStudiesSubclassGaleaspidaHalsteadTarl0,l9670rderHuanana8piformesJanvier,l975FamilyGantar0stra8pidaeWangetWang,l992GenusCantarostraspisWangetWang,l992CQntarostra8pisgeniWangetWang,l992(Fig.l3Pl-II,4-5)1992,Gantarostraspisgeni,Wang,J.Q.etWang,N.Z.,Fig-l,Pl.l.HolotypeAdorsalshieldV9758,LowerDevonianPosongchongF0rmation,Gu-mu,Wenshandistrict,Yunnan-ReferredspeeimensAdorsalshieldVlO494.1;arostra1pr0cessV1O494.2.Loca1ityandh0riz0nXujiach0ng,Xishan,Qujing,Yunnan,Lowe…     

Antimicrobial susceptibility and molecular characterization of <Emphasis Type="Italic">Vibrio cholerae</Emphasis> from cholera outbreaks in Chennai

The genotype and antibiotic resistance pattern of the toxigenic Vibrio cholerae strains associated with cholera outbreaks vary frequently. Fifty-one V. cholerae strains isolated from cholera outbreaks in Chennai (2002–2005) were screened for the presence of virulence and regulatory genes by multiplex polymerase chain reaction (PCR) assay. Genotyping of the isolates was done by VC1 primers derived from enterobacterial repetitive intergenic consensus (ERIC)-related sequence in V. cholerae. All the isolates possessed toxigenic genes, such as ctxA, ctxB, tcpA, ace, ompU, toxR and zot. Two different El Tor genotypes and one O139 genotype could be delineated by VC1-PCR. One of the El Tor genotypes was similar to the El Tor strains isolated from Bhind district and Delhi during 2004. Antibiotic susceptibility testing revealed greater variability among the isolates tested. All the isolates were found to be susceptible to norfloxacin, ciprofloxacin and tetracycline. Thiry-three per cent of the isolates were found to be resistant to more than 4 antibiotics and could be termed as multiple antibiotic resistant. Coexistence of O139 serogroup along with the El Tor biotype could be identified among the strains recovered during the period 2002–2004. The O139 isolates were found to be more susceptible to the antibiotics tested when compared to the El Tor isolates.     

Biotype-specific tcpA genes in Vibrio cholerae

Abstract The tcpA gene, encoding the structural subunit of the toxin-coregulated pilus, has been isolated from a variety of clinical isolates of Vibrio cholerae , and the nucleotide sequence determined. Strict biotype-specific conservation within both the coding and putative regulatory regions was observed, with important differences between the El Tor and classical biotypes. V. cholerae O139 Bengal strains appear to have El Tor-type tcpA genes. Environmental O1 and non-O1 isolates have sequences that bind an E1 Tor-specific tcpA DNA probe and that are weakly and variably amplified by tcpA -specific polymerase chain reaction primers, under conditions of reduced stringency. The data presented allow the selection of primer pairs to help distinguish between clinical and environmental isolates, and to distinguish El Tor (and Bengal) biotypes from classical biotypes from classical biotypes of V. cholerae . While the role of TcpA in cholera vaccine preparations remains unclear, the data strongly suggest that TcpA-containing vaccines directed at O1 strains need include only the two forms of TcpA, and that such vaccines directed at (O139) Bengal strains should include the TcpA of El Tor biotype.     

Detection and differentiation of the gene for toxin co-regulated pili (tcpA) in Vibrio cholerae non-O1 using the polymerase chain reaction

Abstract The polymerase chain reaction has been used to differentiate the gene which encodes the toxin co-regulated pili ( tcpA ) of the El Tor and classical biotypes of Vibrio cholerae O1. The same PCR primers were applied to strains belonging to non-O1 serogroups that produced cholera toxin. The size of fragment amplified was either identical to the tcpA of biotype El Tor (471 bp) or to the tcpA of biotype classical (617 bp). All strains belonging to the novel epidemic serogroup O139 generated a 471-bp fragment identical to El Tor tcpA . The present study suggests that there may be an association between non-O1 serogroup and tcpA type.     

天津张贵庄晚二叠世孢粉植物群

天津张贵庄SR13号钻孔原归为石盒子组的地层近顶部发现了丰富的孢粉化石,这一组合兼具上石盒子组和孙家沟组孢粉组合的特征。一方面,它与上石盒子组（特别是其上部）组合有不少共同分子,在蕨类孢子和裸子植物花粉（包括具肋花粉）的含量比例上也可以与之对比,但缺乏一些标志分子。另一方面,在属种组成上,当前组合与已知的孙家沟组合也颇可比较,而且出现了一些中生代色彩的分子。这一组合的揭示与研究有助于提高人们对华北晚二叠世晚期孢粉植物群特征的认识。依据当前组合与已知相关孢粉组合的对比讨论,将其时代定为晚二叠世,并倾向于晚二叠世晚期（对应于孙家沟组）。     

Lower Emsian biostratigraphy and event stratigraphy of Ha Giang Province,northern Vietnam

A new species of rugose coral, Sanidophyllum dubium n. sp., and the typical Emsian (Early Devonian) rugose coral Xystriphylloides nobilis are described from the Mia Le Formation in northern Vietnam. The lower Emsian index conodonts ranging from the Polygnathus excavatus zone to the P. nothoperbonus zone are illustrated. The biostratigraphic correlation between northern Vietnam and South China shows that the Mia Le Formation in northern Vietnam is early Emsian in age, and its upper part can be correlated with the lower part of the Shizhou Member of the Yukiang Formation in Liujing, Guangxi and its equivalents in South China. Based on the study of the lower Emsian biostratigraphic sequence, the disappearance of Xystriphylloides nobilis fauna in the overlying bed of the uppermost Mia Le Formation and the extinction of the “tonkinensis fauna” (sensu lato) in the interval between the basal Si Phai Formation and the uppermost Mia Le Formation demonstrate the influence of the Yujiang Event in northern Vietnam.