一株重组乙型肝炎病毒的全基因克隆和序列分析

通过血清学和PCR方法对广西地区乙型肝炎病毒感染者样本进行检测,发现一株乙型肝炎病毒基因序列与其余病毒差异较大,利用PCR的方法,扩增出该株病毒的全长cDNA序列,并将其克隆到T载体,进行序列测定,结果显示基因全长为3 215bp,血清型为adr.将测定序列与网上公布的标准基因序列进行比对分析,发现该株病毒全基因组序列进化分析结果与C型基因比较接近,而对其全基因组进行分析时发现1 630bp～2 880bp间基因起源与和C型基因最为接近,而其余基因序列则与A型基因更接近,提示这是一株C型和A型重组的乙型肝炎病毒,首次在国内发现这种类型的基因重组病毒,丰富了我国乙型肝炎病毒研究内容,并对基因型别研究和病毒进化研究提供了参考.     

PTTG1通过P53促进HBV的复制

摘要:【目的】探究垂体瘤转化基因1对乙型肝炎病毒复制的影响。【方法】通过酶联免疫吸附反应、实时定量PCR、双荧光报告系统检测、免疫印迹分析,研究垂体瘤转化基因1对乙型肝炎病毒的复制影响及其机制。【结果】发现垂体瘤转化基因1 促进乙型肝炎病毒复制,是通过降低P53的水平,削弱P53对HBV增强子I和II的抑制作用实现的。【结论】垂体瘤转化基因1通过抑制P53,促进乙型肝炎病毒的复制。     

乙型肝炎病毒基因分型的研究进展

对乙型肝炎病毒的研究发现,病毒变异导致的不同基因型具有不同的临床意义。病毒基因型间的氨基酸差异可导致病毒复制水平的不同,会影响患者的抗病毒治疗效果以及耐药现象的产生,故及时检测患者感染的病毒基因型对指导临床工作、预测感染的结果尤为重要。乙型肝炎病毒基因分型技术已成为迫切需要进行研究和普及的重要内容;     

在转化动物细胞中表达乙型肝炎核心抗原与e抗原

本文报告用包含乙型肝炎病毒(HBV)核心抗原基因的重组质粒与带有单纯疱疹病毒胸苷激酶(HSV-TK)基因的质粒pX1共转化小鼠LTK~-细胞,乙型肝炎核心抗原(HBcAg)与e抗原(HBeAg)在小鼠L细胞中获得了表达。 重组质粒HBc-54中包含乙型肝炎病毒ayw亚型BamHI 1.5kb DNA片段,内含全部乙     

microRNA在乙型肝炎病毒感染及相关疾病中的作用

乙型肝炎病毒(hepatitis B virus,HBV)是一种嗜肝性DNA病毒,感染后可导致急性和慢性肝炎,而慢性感染是导致肝硬化、肝癌和肝衰竭的主要病因。在乙型肝炎病毒复制、转录和相关疾病进程中,microRNA(miRNA)扮演着重要的角色。乙型肝炎病毒感染肝细胞后能引起细胞内microRNA表达谱的改变:一方面,microRNA能促进乙型肝炎病毒的转录和诱导宿主细胞向肿瘤细胞转化;另一方面,microRNA也能抑制乙型肝炎病毒包装和复制。重要的是,乙型肝炎病毒的感染能影响宿主血清microRNA的表达。因此,这类特殊的microRNA今后可成为乙型肝炎病毒相关疾病诊断的潜在生物标记物。将对乙型肝炎病毒与宿主microRNA之间相互作用及其相关生物学效应作一综述。     

乙型肝炎病毒e抗原的生物学功能及血清学转换的免疫学基础

目前在临床乙型肝炎的治疗中,乙型肝炎病毒e抗原(HBeAg)消失及其抗体的出现已成为重要的疗效指标.本文回顾了HBeAg的发现及其生物学和医学意义,对HBeAg与乙型肝炎病毒核心抗原(HBcAg)的免疫原性进行了比较,阐述了HBeAg血清学转换的免疫学基础,并对出现HBeAg血清学转换的意义作了分析.     

乙型肝炎病毒前表面抗原(pre-S)编码区基因的功能

编码乙型肝炎病毒表面抗原蛋白质的基因能直接合成389～400个氨基酸的蛋白质。起始的108～119个氨基酸由pre-S2基因编码,其后的55个氨基酸由pre-S1基因编码,最后为S基因编码的226个氨基酸。 对于由pre-S基因所编码的蛋白质的功能,以前研究甚少,直至最近一、二年才对其发生较为广泛的兴趣。在1986年5月冷泉港乙型肝炎分子生物学会议上提出了几篇报告。     

乙型肝炎病毒动物模型研究进展

乙型肝炎病毒感染是全球范围影响人类健康的重要问题,慢性感染人群存在肝硬化和肝细胞癌的高患病风险。尽管乙型肝炎病毒疫苗有效,但是在世界范围内慢性感染人数超过了3.5亿,占全球人数的5%。乙型肝炎病毒的生物学研究及新治疗发展进展缓慢是由于缺乏合适的动物模型,每一种动物模型都有其优势和特殊弊端。简要综述了各种动物模型在乙型肝炎病毒研究中的应用进展。     

乙型肝炎病毒的表面抗原126位Ilie→Ser变异株

乙型肝炎病毒的表面抗原１２６位Ｉｌｉｅ→Ｓｅｒ变异株房德兴,甘人宝,李载平（中国科学院上海生物化学研究所,２０００３１）段恕诚（上海医科大学附属儿科医院,２０００３２）关键词乙型肝炎病毒,Ｓ基因,表面抗原,变异株乙型肝炎病毒（ＨＢＶ）为嗜肝ＤＮＡ病毒...     

HBVX蛋白与基因调控和肿瘤形成的关系

乙型肝炎病毒Ｘ蛋白是乙型肝炎病毒基因组的四个开读框架之一,Ｘ－ＯＲＦ编码一分子量约为１７ｋＤ,约含有１５４个氨基酸的蛋白质。实验证实ＰＸ是一种反式作用因子,能与细胞内多种与转录、基因调节有关的因子结合,广泛激活病毒和细胞的启动子,参与基因调控,与ＨＢＶ慢性感染后所形成的肝细胞肿瘤密切相关。ＰＸ本身不具有直接结合双链ＤＮＡ的能力,它主要是通过蛋白与蛋白间的相互作用来行使功能,其中包括直接与一般转录因     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.