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1.
An efficient synthesis of 12-hetero steroids was achieved via a Baeyer-Villiger oxidation and a photolysis as the key steps. We set out to describe in this paper the first synthesis of 12-aza steroids. The characteristic 1H and 13C NMR spectroscopic features of the synthesized compounds are reported. 相似文献
2.
The swelling of rat liver mitochondria observed after addition of Ca2+, phosphate or valinomycin under suitable experimental conditions is inhibited by 19-nor-ethynyl-testosterone acetate (NEA) in the concentration range from 3 to 60 μm. The inhibition is proportional to NEA concentration and occurs when swelling is supported by oxidation of NAD-linked substrates (malate-glutamate), or endogenous substrate. Little or no inhibition occurs when swelling is supported by succinate oxidation. These observations suggest a site-specific effect near the NADH-flavoprotein portion of the respiratory chain. NEA also inhibits slightly the ATP-dependent contraction of Ca2+ swollen mitochondria, indicating a secondary effect on the energy-transfer mechanism. In contrast to these effects, NEA does not significantly affect: (a) H+ ejection after Ca2+ uptake supported by succinate oxidation; (b) valinomycin-induced swelling supported by ATP addition; (c) Na-acetate-induced swelling, in which the permeability of membranes to Na+ is rate limiting; and (d) loss of endogenous mitochondrial pyridine nucleotide. Other steroids such as androsterone, 17β-estradiol, and testosterone derivatives affect mitochondrial swelling like NEA, though to a lesser extent. Effects (a) and (d) are at variance with a previously postulated increase of mitochondrial permeability by steroids, accompanied by swelling. The studies which led to this postulate were carried out at steroid concentrations above 200 μm, where nonspecific effects on membrane permeability may well occur. 相似文献
3.
《Journal of steroid biochemistry》1982,16(1):41-49
In human liver, the oxidation of corticosteroids to 20-hydroxy-21-oic acids proceeds via the formation and oxidation of aldol (20-hydroxy-21-aldehyde) intermediates. Human liver aldehyde dehydrogenases E1 and E2, which we have previously purified to homogeneity, catalyzed the oxidation of steroid aldols by NAD+ to 20-hydroxy-21-oic acids. The hydroxy acids formed after oxidation of the aldol isomer of cortisol (isocortisol) or of 11-deoxycorticosterone (isoDOC) by E1 and E2 respectively, were identified by the criteria of chromatographic mobility, derivatization, and reverse isotope dilution of 4-14C labeled acid end products. Both enzymes showed broad substrate specificity and oxidized both 17-hydroxy and 17-deoxy steroids, though at widely varying rates. Kinetic analysis of the course of oxidation of isocortisol and isoDOC by NAD+ gave intersecting initial velocity plots that conform with a sequential mechanism. The inhibition patterns for both enzymes with thionicotinamide adenine dinucleotide or chloral hydrate were consistent with random sequential behavior. 相似文献
4.
《Chemistry and physics of lipids》1986,40(1):57-69
Evidence is presented which shows that in the preparation of radiolabeled cholesterol and other Δ5-sterols via the technique of tritium exchange-labeling of keto steroids and their subsequent conversion to sterol, 3H is introduced primarily into positions 2 and 6 ([2,2,4.6-3H]-cholest-5-en-3β-ol) rather than exclusively at 2 and 4 as the literature claims. The stoppered-vial method using sodium methoxide versus column using basic alumina was the preferred method of labeling. It gave labeled keto steroids of reproducible specific activity indicative of the number of exchangeable protons. Because the initial oxidation step is critical to the preparation of 3H-sterols especially on the small scale, four common oxidants: pyridinium chlorochromate (Corey reagent), silver carbonate on Celite (Fetizon reagent), aluminum isopropoxide (modified Oppenhauer reagent) and chromic acid (Jones reagent) were compared using as starting materials, cholesterol, cholest-8-en-3β-ol, ergosterol, 24-methylenecholesterol, 24(25)-dihydrolanosterol, lanosterol, sitosterol and stigmasterol. The effectiveness of the oxidant was markedly influenced by the molecular features of the sterol. 相似文献
5.
《Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism》1990,1042(2):241-246
Glycyrrhetic acid, derived from a main component of liquorice, was converted to 3-ketoglycyrrhetic acid reversibly by rat liver homogenates in the presence of NADPH or NADP+. Glycyrrhetic acid-oxidizing and 3-ketoglycyrrhetic acid-reducing activities were localized in microsomes among the subcellular fractions of rat liver. Glycyrrhetic acid-oxidizing activity and 3-ketoglycyrrhetic acid-reducing activities showed pH optima at 6.3 and 8.5, respectively, and required NADP+ or NAD+ and NADPH or NADH, respectively, indicating that these activities were due to glycyrrhetinate dehydrogenase. The dehydrogenase was not solubilized from the membranes by the treatment with 1 M NaCl or sonication, indicating that the enzyme is a membrane component. The dehydrogenase was solubilized with detergents such as Emalgen 913, Triton X-100 and sodium cholate, and then separated from 3β-hydroxysteroid dehydrogenase (5β-androstan-3β-ol-17-one-oxidizing activity) by butyl-Toyopearl 650 M column chromatography. Partially purified enzyme catalyzed the reversible reaction between glycyrrhetic acid and 3-ketoglycyrrhetic acid, but was inactive toward 3-epiglycyrrhetic acid and other steroids having the 3β-hydroxyl group. The enzyme required NADP+ and NADPH for the highest activities of oxidation and reduction, respectively, and NAD+ and NADH for considerable activities, similar to the results with microsomes. From these results the enzyme is defined as glycyrrhetinate dehydrogenase, being quite different from 3β-hydroxysteroid dehydrogenase of Ruminococcus sp. from human intestine, which is active for both glycyrrhetic acid and steroids having the 3β-hydroxyl group. 相似文献
6.
Michael J. Kessler 《Steroids》1982,39(1):21-32
Rapid separation of gonadal steroids in the progesterone(Δ4) and pregnenolone pathway (Δ5) has been accomplished by the use of high performance liquid chromatography (HPLC). Two HPLC systems are utilized. The first requires the use of two separate radial compression columns (C-18 and C-8), with steroids being eluted with a methanol-water gradient. The second employs a stainless steel C-18 (reversed phase) column with a 12% octadecylsilane coating. The latter system separates seven of the eight steroids in the Δ4 and Δ5 pathways in thirty-five minutes. For the quantitation of steroids directly, integration of the peak areas, using 254 nm absorption for the Δ4 pathway steroids (5 ng minimum limit), and 210 nm absorption for the Δ 5 pathway steroids (25 ng minimum limit) is used. For the quantitation of radiolabeled metabolites resulting from incubation of gonadal tissue with radiolabeled steroid precursors, either one of two methods is used: (1) the eluent can be recovered from the HPLC using a fraction collector, and counted in liquid scintillation counter or (2) the entire eluent (or a portion of it) can be counted immediately by directing the flow through a radioactivity detector. 相似文献
7.
Takuya Makino Yohei Katsuyama Toshihiko Otomatsu Norihiko Misawa Yasuo Ohnishi 《Applied and environmental microbiology》2014,80(4):1371-1379
Cytochrome P450 monooxygenases (P450s), which constitute a superfamily of heme-containing proteins, catalyze the direct oxidation of a variety of compounds in a regio- and stereospecific manner; therefore, they are promising catalysts for use in the oxyfunctionalization of chemicals. In the course of our comprehensive substrate screening for all 27 putative P450s encoded by the Streptomyces griseus genome, we found that Escherichia coli cells producing an S. griseus P450 (CYP154C3), which was fused C terminally with the P450 reductase domain (RED) of a self-sufficient P450 from Rhodococcus sp., could transform various steroids (testosterone, progesterone, Δ4-androstene-3,17-dione, adrenosterone, 1,4-androstadiene-3,17-dione, dehydroepiandrosterone, 4-pregnane-3,11,20-trione, and deoxycorticosterone) into their 16α-hydroxy derivatives as determined by nuclear magnetic resonance and high-resolution mass spectrometry analyses. The purified CYP154C3, which was not fused with RED, also catalyzed the regio- and stereospecific hydroxylation of these steroids at the same position with the aid of ferredoxin and ferredoxin reductase from spinach. The apparent equilibrium dissociation constant (Kd) values of the binding between CYP154C3 and these steroids were less than 8 μM as determined by the heme spectral change, indicating that CYP154C3 strongly binds to these steroids. Furthermore, kinetic parameters of the CYP154C3-catalyzed hydroxylation of Δ4-androstene-3,17-dione were determined (Km, 31.9 ± 9.1 μM; kcat, 181 ± 4.5 s−1). We concluded that CYP154C3 is a steroid D-ring 16α-specific hydroxylase which has considerable potential for industrial applications. This is the first detailed enzymatic characterization of a P450 enzyme that has a steroid D-ring 16α-specific hydroxylation activity. 相似文献
8.
The electrical conductivity of sixteen steroids in the solid state was measured as a function of temperature. While too small to measure at room temperature, the exponential increase in conductivity with temperature can be determined at elevated temperatures (70° - 130°C). Most steroids tested had a conductivity of 10-13 to 10-15 ohm-1 cm-1 at 100°C and an activation energy of 2 to 4 ev. It it concluded that the observed conductivity is due to impurities. Exposure of keto steroids to iodine vapor results in unstable colored compounds, probably charge transfer complexes, which in some cases have a conductivity as high as 10-4 ohm-1 cm-1. 相似文献
9.
Sertoli cells from 17 day old rats were shown to convert [14C]acetate to [14C]-labelled cholesterol, pregnenolone and 17α-hydroxypregnenolone. Identification was by several systems of thin layer and gas chromatography of the extracted steroids and their sylil and acetyl derivatives and by recrystallizations with authentic and acetylated unlabelled steroids. Several other steroids formed from acetate were tentatively identified. No androstenedione or testosterone were formed. That the Sertoli cell cultures were free of Leydig cells was established by the absence of histochemically detectable 3β-hydroxysteroid dehydrogenase activity and the inability of the cultures to oxidize the 3β-hydroxyl group of [14C]pregnenolone. This is the first direct evidence that Sertoli cells have the capacity to synthesize steroids from acetate. 相似文献
10.
It is now generally accepted that non‐genomic steroids action precedes their genomic effects by modulation of intracellular signaling pathways within seconds after application. Ca2+ is a very potent and ubiquitous ion in all cells, and its concentration is precisely regulated. The most sensitive on Ca2+ increase is ATP‐consuming plasma membrane calcium pump (PMCA). The enzyme is coded by four genes, but isoforms diversity was detected in excitable and non‐excitable cells. It is the only ion pump stimulated directly by calmodulin (CaM). We examined the role of PMCA isoforms composition and CaM effect in regulation of Ca2+ uptake by estradiol, dehydroepiandrosterone (DHEA), pregnenolone (PREG), and their sulfates in a concentration range from 10?9 to 10?6 M, using the membranes from rat cortical synaptosomes, differentiated PC12 cells, and human erythrocytes. In excitable membranes with full set of PMCAs steroids apparently increased Ca2+ uptake, although to a variable extent. In most of the cases, CaM decreased transport by 30–40% below controls. Erythrocyte PMCA was regulated by the steroids somewhat differently than excitable cells. CaM strongly increased the potency for Ca2+ extrusion in membranes incubated with 17‐β‐estradiol and PREG. Our results indicated that steroids may sufficiently control cytoplasmic calcium concentration within physiological and therapeutic range. The response depended on the cell type, PMCA isoforms expression profile, CaM presence, and the steroids structure. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
11.
High Pressure liquid chromatographic procedures for the resolution of mixtures of C18 and C19 steroids of biologic importance are described. On-line monitoring of the absorbance and refractive index of the eluate was found to be suitable for the quantitative measurement of the amounts of eluted steroids. Using a high sensitivity absorbance detector, reliable measurements of as little as 5 ng Δ4?3 keto steroids were possible. 相似文献
12.
Ch.V. Rao 《Prostaglandins & other lipid mediators》1975,9(4):569-578
The specific binding of 3H-prostaglandin E1 (3H-PGE1) to bovine corpus luteum cell membranes was not affected by cholesterol or various progestins at concentrations of up to 9.0 × 10−6M. At concentrations above 2.5 × 10−6M; estrone, 17β-estradiol (but not 17α-estradiol or 17β-estradiol glucuronide), estriol, equilin, D-equilenin, 17-ethynyl estradiol, diethylstilbestrol, cortisol, corticosterone, deoxycorticosterone and aldosterone inhibited specific binding of 3H-PGE1. On the other hand, testosterone and dihydrotestosterone (DHT) (but not androstenedione) significantly enhanced 3H-PGE1 binding. These findings permitted the following correlations between steroid structure and modulation of 3H-PGE1 binding: steroids with a free phenolic ring and a 17β-hydroxyl or 17-keto group or C-21 steroids with a C-20 ketone and a C-21 hydroxy group decrease, whereas C-19 steroids with a C-17 hydroxy group enhance specific binding of 3H-PGE1. PGE receptors are heterogeneous with respect to affinity for 3H-PGE1. The steroids that decreased 3H-PGE1 binding caused a lowering to a complete loss of low affinity PGE receptors. Steroids that increased 3H-PGE1 binding caused appearance of new low affinity PGE receptors. Association rate constants for 3H-PGE1 binding were decreased by 17β-estradiol (61%) and increased by DHT (59%). 相似文献
13.
Charles L. Bevins Robert H. Kayser Ralph M. Pollack D.Belinda Ekiko Scott Sadoff 《Biochemical and biophysical research communications》1980,95(3):1131-1137
The spiro-17β-oxiranyl derivatives of d-equilenin, epiandrosterone, dehydroepiandrosterone, and Δ4-androsten-3,17-dione are active-site-directed irreversible inhibitors of Δ5-3-ketosteroid isomerase of . The 17β-oxiranyl steroids rapidly inhibit the isomerase in a time-dependent manner which exhibits saturation kinetics. The enzyme is protected against inactivation by the competitive inhibitor 19-nortestosterone. In addition, prolonged dialysis against neutral buffer leads to no regeneration of enzyme activity. Fluorescent spectral changes associated with the incubation of the enzyme with the 17β-oxirane derived from d-equilenin indicate that there are two modes of binding for steroids to the isomerase. These results suggest that 17β-oxiranes may inhibit the enzyme by a mechanism similar to that for the previously studied spiro-3β-oxiranyl steroids. 相似文献
14.
Substrate and inhibitor specificity of the cholesterol oxidase in bovine adrenal cortex 总被引:2,自引:2,他引:0
1. Cholesteryl 3β-sulphate is oxidized in vitro by preparations of bovine adrenal-cortex mitochondria to pregnenolone sulphate and isocaproic acid (4-methyl-pentanoic acid) without hydrolysis of the ester linkage. 2. Free cholesterol is the preferred substrate for adrenal-cortex cholesterol oxidase; the apparent Km for cholesteryl sulphate is 500μm and for free cholesterol 50μm under the same conditions. 3. Cholesteryl 3β-acetate is hydrolysed by bovine adrenal-cortex mitochondria in vitro to free cholesterol, which is subsequently oxidized to more polar steroids and isocaproic acid. Evidence was obtained that other cholesterol esters behave similarly. Cholesterol esters may thus act as precursors of steroid hormones. 4. Cholest-4-en-3-one is only poorly oxidized to isocaproic acid and more polar steroids and thus is probably not a significant precursor of steroid hormones. 5. Cholesteryl esters inhibit the oxidation of cholesterol competitively (Ki for cholesteryl phosphate 28μm, for cholesteryl sulphate 110μm, for cholesteryl acetate 65μm) but pregnenolone esters do not inhibit this system. 6. Pregnenolone and 20α-hydroxycholesterol (both metabolites of cholesterol in this system) inhibit the oxidation of cholesterol non-competitively. Ki for pregnenolone is 130μm and Ki for 20α-hydroxycholesterol is 17μm. 7. 25-Oxo-27-norcholesterol inhibits cholesterol oxidation non-competitively (Ki16μm). A number of other Δ5-3β-hydroxy steroids inhibit cholesterol oxidation and evidence was obtained that the 3β-hydroxyl group was necessary for inhibitory activity. 8. Pregnenolone, 20α-hydroxycholesterol and 25-oxo-27-norcholesterol inhibit oxidation of cholesteryl sulphate by this system but their sulphates do not. 9. 3β-Hydroxychol-5-enoic acid, 3α-hydroxy-5β-cholanic acid and 3β-hydroxy-22,23-bisnorchol-5-enoic acid stimulated formation of isocaproic acid from cholesterol. 10. No evidence was obtained that phosphorylation or sulphation are obligatory steps in cholesterol oxidation by adrenal-cortex mitochondria. 11. The cholesteryl 3β-sulphate sulphatase of bovine adrenal cortex was found mostly in the microsomal fraction and was inhibited by inorganic phosphate. 相似文献
15.
A. Christy Hunter Kieran R. Watts Cinzia Dedi Howard T. Dodd 《The Journal of steroid biochemistry and molecular biology》2009,116(3-5):171-177
A series of steroids (progesterone, testosterone acetate, 17β-acetoxy-5α-androstan-3-one, testosterone and androst-4-en-3,17-dione) have been incubated with the thermophilic ascomycete Myceliophthora thermophila CBS 117.65. A wide range of biocatalytic activity was observed with modification at all four rings of the steroid nucleus and the C-17β side-chain.This is the first thermophilic fungus to demonstrate the side-chain cleavage of progesterone. A unique fungal transformation was observed following incubation of the saturated steroid 17β-acetoxy-5α-androstan-3-one resulting in 4-hydroxy-3,4-seco-pregn-20-one-3-oic acid which was the product generated following the opening of an A-homo steroid, presumably by lactonohydrolase activity. Hydroxylation predominated at axial protons of the steroids containing 3-one-4-ene ring-functionality. This organism also demonstrated reversible acetylation and oxidation of the 17β-alcohol of testosterone.All steroidal metabolites were isolated by column chromatography and were identified by 1H, 13C NMR, DEPT analysis and other spectroscopic data. The range of steroidal modification achieved with this fungus indicates that these organisms may be a rich source of novel steroid biocatalysis which deserve greater investigation in the future. 相似文献
16.
Ch. V. Rao 《Prostaglandins & other lipid mediators》1975,9(4):579-584
The specific binding of 3H-prostaglandin E1 (3H-PGE1) to bovine corpus luteum cell membranes was not affected by cholesterol or various progestins at concentrations of up to 9.0 × 10−6M. At concentrations above 2.5 × 10−6M; estrone, 17β-estradiol (but not 17α-estradiol or 17β-estradiol glucuronide), estriol, equilin, D-equilenin, 17-ethynyl estradiol, diethylstilbestrol, cortisol, corticosterone, deoxycorticosterone and aldosterone inhibited specific binding of 3H-PGE1. On the other hand, testosterone and dihydrotestosterone (DHT) (but not androstenedione) significantly enhanced 3H-PGE1 binding. These findings permitted the following correlations between steroid structure and modulation of 3H-PGE1 binding: steroids with a free phenolic ring and a 17β-hydroxyl or 17-keto group or C-21 steroids with a C-20 ketone and a C-21 hydroxy group decrease, whereas C-19 steroids with a C-17 hydroxy group enhance specific binding of 3H-PGE1. PGE receptors are heterogeneous with respect to affinity for 3H-PGE1. The steroids that decreased 3H-PGE1 binding caused a lowering to a complete loss of low affinity PGE receptors. Steroids that increased 3H-PGE1 binding caused appearance of new low affinity PGE receptors. Association rate constants for 3H-PGE1 binding were decreased by 17β-estradiol (61%) and increased by DHT (59%). 相似文献
17.
HeLa cells grown in chemically defined medium lacking glucocorticoids synthesize metallothioneins, low molecular-weight heavy-metal binding proteins. Dexamethasone and hydrocortisone increase the rate of metal-lothionein synthesis five- to ten-fold. Maximal induction is achieved with 10–8M dexamethasone and 10–7M hydrocortisone. Half-maximal induction is achieved at 5 ± 10–9M dexamethasone and 5 ± 10–8M hydrocortisone. Although carried for many generations in the absence of any glucocorticoids, HeLa cells (clone S) contain 25,000 specific 3H-dexamethasone receptors that translocate into the nucleus after one hour of incubation. 3H-dexamethasone binds to a single class of receptors with an apparent Kd = 18.8 nM. A variety of steroids can be classified into three classes, based on their effect on metallothionein synthesis: (a) full agonists (optimal inducers), (b) intermediate effectors which have either partial agonist or antagonist activities, and (c) inactive steroids. There is a correlation between the effects on metallothionein synthesis of different steroids and their ability to compete with 3H-dexamethasone binding. We conclude that metallothionein is induced in HeLa cells by a glucocorticoid receptor mediated mechanism. 相似文献
18.
《Journal of steroid biochemistry》1982,16(2):237-243
A quantitative cytochemical method for the demonstration of 20α-hydroxysteroid dehydrogenase activity (20α-HSD) in the regressing corpora lutea of the adult rat ovary is described. The method employs unfixed tissue sections and relies upon the oxidation of 20α-hydroxy-4-pregnen-3-one (20α-OH-P) with nitro blue tetrazolium as the hydrogen acceptor. The enzyme was dependent upon NADP+ for its activity and was inactive when 20β-hydroxy-4-pregnen-3-one (20β-OH-P) was used as a substrate. The apparent Km values for 20α-OH-P and NADP+ were 3 × 10−4M and 2.5 × 10−5M respectively. Inhibition of 20α-HSD activity by steroids was demonstrable at pH 8. Androstenedione was by far the most potent inhibitor, followed by progesterone (the product of the enzyme activity) 17α-hydroxyprogesterone. Compound S and 20β-OH-P. At pH 6.8, a pH more favourable to the progesterone → 20α-OH-P reaction, only progesterone and 17α-hydroxyprogesterone were inhibitory. Testosterone was without demonstrable effect at either pH. 相似文献
19.
Antibody 1E9, which was elicited with a hexachloronorbornene derivative and catalyzes the Diels-Alder reaction between tetrachlorothiophene dioxide and N-ethylmaleimide with high efficiency, was successfully reengineered to bind a range of structurally diverse steroids with nanomolar affinities. Remarkably, two mutations (LeuH47Trp/ArgH100Trp) out of 36 total sequence differences suffice to switch the selectivity of 1E9 to that of the progesterone-binding antibody DB3. In contrast to the double mutant, which tightly binds multiple steroids with differently configured A-B ring junctions, the individual LeuH47Trp and ArgH100Trp single mutants both exhibit significantly greater specificity than DB3, preferentially binding 5α-pregnan-3β-ol-20-one (Kd ≈ 5 nM) over other steroids. These findings illustrate how easily differently shaped binding pockets can be created through subtle changes to the same primordial germ line template. 相似文献
20.
Dóra Ondré János Wölfling István Tóth Mihály Szécsi János Julesz Gyula Schneider 《Steroids》2009,74(13-14):1025-1032
17β-Oxazolinyl steroids 7a–g and 8a–g were synthesized. The Lewis acid-catalysed reactions of (20R)-3β-acetoxy-21-azidomethyl-20-hydroxypregn-5-ene with substituted aromatic aldehydes led to the formation of 3β-acetoxyandrost-5-enes substituted in position 17β with oxazolinyl residues (7a–g). Oppenauer oxidation of the 3β-hydroxy-exo-heterocyclic steroids yielded the corresponding Δ4-3-ketosteroids. The inhibitory effects (IC50) of both 3-hydroxy compounds 7a–g and their Δ4-3-keto counterparts 8a–g on rat testicular C17,20-lyase were investigated with an in vitro radioligand incubation technique. The 3-chlorophenyl- (8d), and the 4-bromophenyl-17β-(2-oxazolin-5-yl)androst-4-en-3-one derivatives (8f) were found to be modest inhibitors (IC50 = 4.8 and 5.0 μM, respectively). 相似文献