Coremiella cuboidea (Sacc. et Ell.) Cif. et Car. n. comb. isolata da feci umane

Riassunto Sono studiati nelle loro caratteristiche morfo-culturali e biologiche quattro ceppi omogenei diCoremiella isolati da feci umane. Questo genera è simile aGeotrichum ma oltre a cellule miceliche clamidosporo-simili presenta formazione apicali miceliche di tipo coremioide reptante.L'artrosporulazione è un processo endogeno come inGeotrichum, e come inGeorichum si hanno artrospore clamidosporioidi fertili opache e artrospore usuali non fertili e jaline, ma meno differenziate.I ceppi vengono riferiti aC. ulmariae e le caratteristiche vengono paragonate anche con le culture su legno diSpirea ulmaria.Il primo esemplare diCoremiella è stato pubblicato comeOospora cuboidea Sacc. etEll. come è stato accertato studiando l'esemplare autentico diEllis.In conclusione il genereCoremiella Bub. etKrieg. è stato emendato ed è stata creata la combinazioneC. cuboidea (Sacc. etEll.).

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Summary Four homogeneous strains ofCoremiella from human feces were studied from morpho-cultural and biological standpoint. This genus is likeGeotrichum, but in addition to some mycelial, chlamydospore-like cells, coremioid mycelial, reptant strands are formed.The arthrosporulation is an endogeneous process, and, likeGeotrichum, there are usual hyaline and duller chlamydosporioid, fertile arthrospores, but less differentiated.The strains are referred toC. ulmariae, and the characteristics were compared also on timber and woody European Meadowsweet cultures. But the first binomial ofCoremiella has been published asOospora cuboidea Ell. etSacc., as ascertained by the study of the authenticEllis specimen.In conclusion, the genusCoremiella Bub. etKrieg. has been emended, and the combinationC. cuboidea (Sacc. etEll.) established.

     

Appressorien und Chlamydosporen von Colletotrichum atramentarium (Berk. et Br.) Taub.

Zusammenfassung Colletotrichum atramentarium (Berk. et Br.) Taub. bildet neben den lange Zeit bekannten Appressorien auch Chlamydosporen, die erstmalig von Ettig (1958) beschrieben wurden. Beide Organe unterscheiden sich in ihrer Genese und Funktion, sowie in Form und Größe voneinander. Die Appressorien werden oft irrtümlich als Chlamydosporen bezeichnet.     

Variation in Copper Tolerance among Homokaryotic and Dikaryotic Mycelia of Polyporus palustris Berk. et Curt.

Investigations were made of the variation in copper toleranceamong homokaryons and dikaryons originating from one dikaryoticisolate of Polyporus palustris, as shown by their growth onnutrient agar containing copper sulphate, usually at 0.32 Mconcentration (c. 8 per cent of CuSO₄.5H₂O). Homokaryons obtainedby monobasidiospore isolation were crossed and further homokaryonsobtained in the same way. Selection for copper tolerance gavean increase in the second generation, but no further increasein the third; whilst selection for copper sensitivity gave substantialincreases in both second and third generations. It is concludedthat copper tolerance in this fungus is controlled by a numberof genes. It was found that, in this fungus, the copper toleranceof a dikaryon was substantially greater than that of eitherof its constituent homokaryons. It was also found that differentisolates of P. palustris varied very widely in copper tolerance.     

A simple technique for estimation ofDrechslera nodulosa (Berk. & Curt.) Subram. and Jain propagules in soil

Summary A baiting technique was developed to estimate the population ofDrechslera nodulosa (Berk. and Curt.) Subram. and Jain in soil by using susceptible ragi (Eleusine coracana Gaertn.) culms. The number of lesions developed on baited culms were reduced with the reduction in concentration ofD. nodulosa propagules in both sterilized and unsterilized soils. Based on this a standard correlation (concentrationvs infection probability) was established which was found to be quite efficient method to estimate the population in soil and to bait even when the inoculum level was 4 propagules per g of soil.     

Isolation ofGlomerella musae [teleomorph ofColletotrichum musae (Berk. & Curt.) Arx.] and segregation analysis of ascospore progeny

The sexual stage ofColletotrichum musae has been obtained under controlled laboratory conditions. Development of the sexual stage required light and suboptimal growth temperature. Meiosis was apparent as judged by the formation of perithecia typical of ascomycetes classified asGlomerella. Perithecia contained as many as 50–80 asci, each of which contained up to eight ascospores. The ascospores were larger than conidia and were easily dissected for segregation analysis of either random spores or tetrads. In order to determine if this meiotic system was homothallic or heterothallic, chlorate-resistant mutants were isolated and crossed with wild type. Chlorate resistance and susceptibility segregated in a 1:1 ratio, indicating that this system was heterothallic. Sexual recombination was observed by analyzing the segregation of several DNA markers identified by amplification of polymorphic fragments of genomic DNA using single oligonucleotide primers andTaq DNA polymerase. Segregation consistent with mendelian inheritance and sexual recombination of genetic markers indicated that it is feasible to construct a genetic map for this species. The complexity of sexual compatibility was analyzed by crossing several ascospore progeny which identified four distinct mating types.     

Characterization of an extracellular glycolipid from Lentinus edodes (Berk.) Sing [Lentinula edodes (Berk.) Pegler]

Submerged mycelium of a xylotrophic basidiomycete Lentinus edodes produces an extracellular glycolipid, S3, associated with a lectin. Galactose glycan residue, as well as the lipid pool composition, which includes nonhydroxylated short-chain fatty acids, is uncommon for basidiomycetes. The glycolipid consists of D-galactopyranose (15% of S3 contains galactose sulfate) acylated by octadecanoic and nonadecanoic fatty acid residues (28 and 72%, respectively). The glycolipid structure and composition are confirmed by physicochemical analysis. The glycolipid is assumed to be a regulator of lectin activity.     

Photosensitization in cattle grazing on pastures of Brahciaria decumbens Stapf infested with Pithomyces chartarum (Berk. & Curt.) M.B. Ellis

Aspects of photosensitization in bovines grazing on pastures of Brachiaria decumbens Stapf infested with Pithomyces chartarum (Berk. & Curt.) M.B. Ellis infested all pastures 45(2):117-136, 1978. This paper reports experimental studies on photosensitization in bovines grazing on different pastures of Brachiaria decumbens Stapf in the "Cerrados" region (Planaltina, DF). Climatic conditions, zinc content and occurence of fungi on pastures were investigated. Pithomyces chartarum (Berk. & Curt.) M.B. Ellis infested all pastures examined. Photosensitization was observed in one animal maintained on a pasture of B. decumbens formed with seeds from Australia. Clinical and necropsy data were similar to those related in literature for sporidesmin-intoxicated animals. An isolate of P. chartarum and samples of bovine bile were assayed for sporidesmin presence.     

Endophytic Fungi in Four Winter Wheat Cultivars (Triticum aestivum L.) Differing in Resistance Against Stagonospora nodorum (Berk.) Cast. & Germ. =Septoria nodorum (Berk.) Berk.

Four winter wheat cultivars with different levels of resistance to Septoria nodorum were investigated at four locations during two vegetation periods. Forty plants per cultivar and site were collected at random at seven defined growth stages from crop emergence to harvest. Samples from roots, culms, leaves, glumes and kernels were examined for the occurrence of endophytic fungi after surface sterilization. 83% of the 26944 isolates sporulated and were assigned to 213 species. The most frequent were: Septoria nodorum (20.1%), Alternaria tenuissima (9.8%), Epicoccum purpurascens (9.1%), Idriella bolleyi (6.9%), Fusarium graminearum (5.3%), Fusarium culmorum (4.0%), Cladosporium oxysporum (3.7%), Didymella exitialis (3.1%), Fusarium nivale (2.8%) and Rhizoctonia solani (2.1%). Each species occurred preferentially in one or more plant organs. A factorial analysis of variance showed that plant organ, sampling site, vegetation, period and cultivar in decreasing order of importance influenced the quantitative and qualitative composition of the fungal populations. No relationship between endophytic fungi was found to be constantly antagonistic or mutualistic. Septoria nodorum was isolated mainly from culms. The number of S. nodorum isolates differed significantly between cultivars in culms and glumes but not in flag leaves. The results are discussed in relation to resistance breeding and the effect endophytic fungi, might have on yield.     

一种含有单链RNA的香菇球状病毒

从生长不正常的香菇(Lentinus edodes(Berk.)Sing)菌株中分离到一种等轴对称含单链RNA的病毒颗粒。病毒颗粒在电镜下直径为33～34nm,在SDS-聚丙烯酰胺凝胶电泳中病毒外壳蛋白分子量为22000道尔顿。病毒核酸径DNase1和SI酶解试验及热变性紫外吸收曲线试验证明为单链RNA,在1.5％的琼脂糖凝胶电泳中,病毒核酸呈现一条带,分子量为2.38×10~6道尔顿。     

Wirkung von Fungiziden auf die Sporulation von Podosphaera leucotricha (Ell. et Ev.) Salm. und Venturia inaequalis (Cooke) Wint

This paper presents the results of 3 year‐long monitoring of crop fields in four ecologically different areas of Ukraine for several phytoviral infections. Various modifications of ELISA were used as main assay to evaluate the spreading of a virus. The technique allowing to determine the frequency of occurrence for a virus of interest is proposed. This technique is based on an identification of viral antigens in crops, accompanying weeds and soil. The differences between the investigated regions on a virus occurrence frequency as well as dynamics of the parameter in relation to crop‐rotation have been revealed. The possibility to use results obtained for predicting phytoviral disease spreading is discussed.     

Morphological differentiation ofFusarium sambucinum Fuckel sensu stricto,F. torulosum (Berk. & Curt.) Nirenberg comb. nov. andF. venenatum Nirenberg sp. nov.

WithinFusarium sambucinum Fuckel sensu lato three species were differentiated:F. sambucinum Fuckel s. str.,F. torulosum (Berk. et Curt.) Nirenberg comb. nov. andF. venenatum Nirenberg sp. nov. They are described and illustrated in detail.     

Beobachtungen anCordyceps sinensis (Berk.) Sacc. und verwandten Pilzen

Ohne Zusammenfassung