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1.
- A fully automated phototaxis monitoring device is described for measuring photo-topatactic responses of flagellated organisms.
- Photokinesis can be demonstrated in Chlamydomonas cells only after a dark period of about 72 hrs.
- Pre-darkening of a few hours duration raises the phototactic disposition, whereas pre-illumination has no significant effect.
- Circadian rhythms can be initiated by only one period of darkness or lower light intensity, whereas a period of higher intensity does not induce rhythms. The period length of the circadian rhythms is about 24 hrs.
2.
Genjirou Nishi 《Ichthyological Research》1990,37(2):170-181
Under controlled laboratory conditions, the locomotor activity rhythms of four species of wrasses (Suezichthys gracilis, Thalassoma cupido, Labroides dimidiatus andCirrhilabrus temminckii) were individually examined using an actograph with infra-red photo-electric switches in a dark room at temperatures of 21.3–24.3°C,
for 7 to 14 days. The locomotor activity ofS. gracilis occurred mostly during the light period under a light-dark cycle regimen (LD 12:12; 06:00-18:00 light, 18:00-06:00 dark).
The locomotor activity commenced at the beginning of the light period and continued until a little before the beginning of
dark period. The diel activity rhythm of this species synchronizes with LD. Under constant illumination (LL) this species
shows distinct free-running activity rhythms varying in length from 23 hrs. 39 min. to 23 hrs. 47 min. Therefore,S. gracilis appears to have a circadian rhythm under LL. However, in constant darkness (DD), the activity of this species was greatly
suppressed. All the fish showed no activity rhythms in DD conditions. After DD, the fish showed the diel activity rhythm with
the resumption of LD, but this activity began shortly after the beginning of light period. The fish required several days
to synchronize with the activity in the light period. Therefore,S. gracilis appeared to continue the circadian rhythm under DD. InT. cupido, the locomotor activity commenced somewhat earlier than the beginning of the light period and continued until the beginning
of the dark period under LD. The diel activity rhythm of this species synchronizes with LD. Under LL, four of the five specimens
of this species tested showed free-running activity rhythms for the first 5 days or longer varying in length from 22 hrs.
54 min. to 23 hrs. 39 min. Although the activity of this species was suppressed under DD, two of five fish showed free-running
activity rhythms throughout the experimental period. The lengths of such free-running periods were from 23 hrs. 38 min. to
23 hrs. 50 min. under DD. Therefore, it was ascertained thatT. cupido has a circadian rhythm. InL. dimidiatus, the locomotor activity rhythm under LD resembled that observed inT. cupido. The diel activity rhythm of this species synchronizes with LD. Under LL, four of seven of this species showed free-running
activity rhythms throughout the experimental period. The lengths of such free-running periods were from 23 hrs. 07 min. to
25 hrs. 48 min. Although the activity of this species was suppressed under DD, three of five fish showed free-running activity
rhythms throughout the experimental period. The lengths of such free-running periods were from 23 hrs. 36 min. to 23 hrs.
41 min. under DD. Therefore, it was ascertained thatL. dimidiatus has a circadian rhythm. Almost all locomotor activity of C.temminckii occurred during the light period under LD. The diel activity rhythm of this species coincides with LD. Under LL, two of four
of this species showed free-running activity rhythms throughout the experimental period. The lengths of such free-running
periods were from 23 hrs. 32 min. to 23 hrs. 45 min. Although the activity of this species was suppressed under DD, one of
the four fish showed free-running activity rhythms throughout the experimental period. The length of the free-running period
was 23 hrs. 21 min. under DD. Therefore,C. temminckii appeared to have a circadian rhythm. According to field observations,S. gracilis burrows and lies in the sandy bottom whileT. cupido, L. dimidiatus, andC. temminckii hide and rest in spaces among piles of boulders or in crevices of rocks during the night. It seems that the differences in
nocturnal behavior among the four species of wrasses mentioned above are closely related to the intensity of endogenous factors
in their locomotor activity rhythms. 相似文献
3.
G. Roblin 《Biological Rhythm Research》2013,44(2):89-109
Abstract Endogenous Rhythms in Mimosa pudica L. Leaf Movements. The rhythmic movements performed by the leaves of the “Sensitive plant”, Mimosa pudica L., observed by time lapse photography, result of periodical turgor variations taking place in the parenchymatous cells of specialized motor organs. These turgor variations are associated with membrane permeability changes and ionic movements. These leaf movements allow to specify the temporal organization of this plant. Statistical analysis of observed periodicities in leaf movement shows that, in alternating conditions of light and dark (L/D:14/10) three distinct rhythms exist: a circadian rhythm synchronized by the photoperiodic cycle (τ = 24 hrs), and two ultradian rhythms with mean period values 3.8 hrs and 0.5 hrs respectively. In constant conditions from germination (L/L), the leaf behavior is strongly modified, but the three period values are found again (mean period values of 25.1 hrs, 3.5 hrs and 0.6 hrs respectively). The occurence of many rhythms with various periods taking place in the same organ is discussed in reference to observations effected on other biological subjects. Then, it appears that the period value within 2 and 4 hrs may be considered as a characteristic one in plants. 相似文献
4.
Locomotor activity rhythms in the crayfish, Faxonella clypeata, were recorded under conditions of controlled light and temperature. In LD 12:12, dark active rhythms with a major activity onset at lights-off, and bimodally active rhythms with onsets at both lights-on and lights-off were recorded. In DD, most of the LD dark active rhythms retained the lights-off activity onset. However, among the majority of the LD bimodally active crayfish, only the lights-on activity peak persisted in DD. A small number of the LD bimodal rhythms remained bimodal in DD. DD records revealed free-running period lengths both greater and less than 24 hrs. An hypothesis stating that the two recorded LD rhythms represent two basically different rhythmic types is presented. The dark active crayfish appear to entrain to the lights-off position, but the bimodally rhythmic crayfish appear to entrain to the lights-on position. 相似文献
5.
G Hildebrandt 《Journal of physiology and pharmacology》1991,42(1):5-27
The spectrum of biological rhythms exhibits characteristic principles of biological time structure which also rule the functional behaviour. With increasing period lengths the rhythms become increasingly complex. In the long-wave section the rhythmic functions find their corresponding cycles in the environment, whereas the shorter waves represent only endogenous autonomous rhythms, which maintain an internal time order by means of frequency- and phase-coordination. Under resting conditions and in a state of complete adaptation only a few spontaneous rhythms dominate in the spectrum. However, under loading conditions as well as in pathological situations further periodicities come up. The spectrum of rhythms can be divided into certain blocks, with the period lengths predominating in each of these whole number frequency ratios forming a harmonic system. Frequency- and phase coordination establish a system of co-action which favours the functional economy of the organism. A tripartite organization of the autonomous rhythms involves different functional behaviours with regard to frequency, amplitude, and phase. Slower rhythms act upon the faster rhythms preferably by modulating their frequencies, while changes of the faster rhythms influence the slower ones by enhancing their amplitudes, multiplying their period lengths and shifting their phases. In principle the reactions of living systems are periodically structured. Reactive periodicity brings to appearance an endogenous time structure, which prefers whole number relationships with the spontaneous rhythms. The phase position of reactive periods depends on the stimulus. The amplitudes dampen down with increasing compensation. From the medical point of view so-called circaseptan (about 7 days) reactive periods are of predominant interest. This periodicity can be observed in numerous adaptive and compensating processes. It does not depend on the external week cycle and was already known to the antiquity. 相似文献
6.
Abstract To test the hypothesis that an oscillator located outside the suprachiasmatic nuclei (SCN) controls the circadian rhythm of body temperature, we conducted a study with 14 blinded rats, 10 of which receiving a SCN lesion. Body temperature was automatically and continuously recorded for about one month by intraperitoneal radio transmitters. Food intake, drinking and locomotor activity were also recorded. Periodograms revealed that 3 rats with histologically verified total bilateral SCN lesions did not exhibit any circadian rhythmicity. The 7 other rats appeared to have partial lesions. They showed shortening of period and severe amplitude reduction in all functions. Thus, no support was found for the hypothesis of a separate circadian ‘temperature oscillator’ located outside the SCN. Nevertheless, after large partial lesions body temperature showed more persistency than some of the other behavioral rhythms. Ultradian rhythms in temperature persisted after partial and total lesions. Other functions showed parallel ultradian rhythms. In intact rats the ultradian peaks were restricted predominantly to the subjective night. After total lesions these peaks became more or less homogeneously distributed in time but more heterogeneously after partial lesions. So the SCN plays a role in the temporal structure of ultradian rhythms but does not generate them. Non‐24‐hour actograms showed instabilities of period and phase of ultradian rhythms. Intact and lesioned rats were similar with respect to the mean (about 3.5 hrs) and standard deviation (about 1.5 hrs) of ultradian periods in temperature. These features indicate that a mechanism outside the SCN is underlying ultradian rhythmicity, capable of generating short‐term oscillations. Two approaches, homeostatic sleep‐wake relaxation oscillations and multiple circadian oscillators, are discussed. 相似文献
7.
S-adenosylmethionine and adenosine levels in the rat pineal gland were determined by high-performance liquid chromatography after fractionation of the pineal extracts. The concentration of S-adenosylmethionine follows a circadian rhythm and is about three times higher during the day (2.5 nmol/gland) than the night (1.1 nmol/gland). The variations in the level of adenosine are apparently more complex. Over the 24 hours period there are two maxima at 03.00 (120 pmol/gland) and 15.00 hrs (100 pmol/gland) and one minimum at 09.00 hrs (50 pmol/gland). In addition, only an ultradian rhythm with a period of 12 hrs and an acrophase of 3 hrs can be evinced by computer analysis. 相似文献
8.
In freely moving rats the diurnal variation in electrolyte excretion was studied. Food was available during either the dark or the light period. The lights were on from 0800–2000; the dark phase extended from 2000–0800 hrs. The electrolyte excretory rhythms were studied during a control period, in which the minerals were present in the food, and during experimental periods, when successive minerals were not present in the food but were instead given by constant intravenous infusion. For both groups the excretory rhythms of K, Mg and P persisted during continuous infusion but the times of maximum and minimum excretion differed. Day-fed animals exhibited a remarkable decrease in amplitude during the mineral infusion period. In contrast, the calcium excretory pattern was only influenced by the feeding period. 相似文献
9.
The circadian rhythms of excretion of sodium, potassium, calcium, magnesium, phosphorus and 17-hydroxycorticosteroid (17-ohcs) were determined in five normal subjects, in six patients with hyperthyroidism and five with hypothyroidism. Constant diets with identical 3-hourly feedings were employed, and urine collections were made every 3 hrs during a 3-day study period. The circadian patterns of urinary excretion of sodium, potassium and 17-OHCS were similar in all three groups with distinct daytime peaks and nighttime nadirs. The total quantities of the ions and 17-OHCS excreted were greater in hyperthyroid than in hypothyroid patients with the greatest difference noted with the 17-OHCS. The rhythms for calcium, magnesium and phosphorus excretion were accentuated in hyperthyroid patients but similar to those in normal subjects with early morning calcium and magnesium peaks and a phosphorus peak approximately 12 hrs later. While a similar although blunted circadian pattern for calcium and perhaps magnesium excretion was noted in hypothyroid patients, their phosphorus rhythms were distorted and rather flat. These latter results confirm the observation of MINTZ et al. and are compatible with their interpretation that thyroid hormone is permissibly necessary for the expression of a normal phosphaturic rhythm and that the circulating level of thyroid hormone influences the amplitude of the phosphaturic rhythm. 相似文献
10.
Heritable fragile sites on human chromosomes. XI. Factors affecting expression of fragile sites at 10q25, 16q22, and 17p12. 总被引:4,自引:2,他引:2
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The fragile sites at 10q25, 16q22, and 17p12 can all be induced in lymphocyte culture by BrdU or BrdC added 6-12 hrs prior to harvest. Without induction, fra(10)(q25) is rarely expressed spontaneously, whereas fra(16)(q22) is frequently expressed spontaneously. Fra(17)(p12) is frequently expressed spontaneously but is probably expressed only after induction in some individuals. Distamycin A, netropsin, and Hoechst 33258 induced high levels of expression of fra(16)(q22) and fra(17)(p12) but did not enhance expression of fra(10)(q25). The mechanisms of induction of fra(16)(q22) by BrdU and distamycin A appear to be different, since the time of induction by BrdU reaches a maximum about 12 hrs prior to harvest whereas induction by distamycin A requires much longer exposure. The fragile sites at 10q25 and 16q22 were both induced in fibroblast culture by BrdU. Fra(17)(p12) is accepted as a fragile site because preliminary studies show that it behaves similarly in lymphocyte culture to fra(16)(q22); however, there is only limited evidence for fragility at 17p12. 相似文献
11.
Effects of light on human circadian rhythms. 总被引:2,自引:0,他引:2
Blind subjects with defective retinal processing provide a good model to study the effects of light (or absence of light) on the human circadian system. The circadian rhythms (melatonin, cortisol, timing of sleep/wake) of individuals with different degrees of light perception (n = 67) have been studied. Blind subjects with some degree of light perception (LP) mainly have normally entrained circadian rhythms, whereas subjects with no conscious light perception (NPL) are more likely to exhibit disturbed circadian rhythms. All subjects who were bilaterally enucleated showed free running melatonin and cortisol rhythms. Studies assessing the light-induced suppression of melatonin show the response to be intensity and wavelength dependent. In contrast to ocular light exposure, extraocular light failed to suppress night-time melatonin. Thus, ocular light appears to be the predominant time cue and major determinant of circadian rhythm type. Optimisation of the light for entrainment (intensity, duration, wavelength, time of administration) requires further study. 相似文献
12.
Franz Pera 《Chromosoma》1968,25(1):21-29
The duration of DNA replication of eu- and heterochromatin in kidney epithelial cell cultures of female Microtus agrestis was determined with combined H3-thymidine pulse labelling and cytophotometric determination of Feulgen DNA. The average duration of the total cell cycle was 23.3 hrs, with a G1 period of 14.6 hrs, S period of 5 hrs, G2 period of 2.7 hrs, and mitosis of 1 hr. The replication time of eu- and heterochromatin was determined by the frequency of the different labelling patterns after pulse labelling. The time sequence of the labelling patterns was ascertained by DNA measurements. During the S period, euchromatin replicates at first alone for 3 hrs (60% of the length of S) and 1 hr (19.3%) together with heterochromatin. During the last hour (20.7%), only heterochromatic regions replicate. The sex chromatin part of the one X chromosome starts synthesis 20 minutes (7.3% of S) before the remainder of the heterochromatic X material and ends 30 minutes (9.7% of S) prior to the termination of the S period. Replication of euchromatin takes about 80% of the duration of the total S period, whereas that of heterochromatin takes only 40%.
Mit dankenswerter Unterstützung durch die Deutsche Forschungs-Gemein-schaft. 相似文献
Mit dankenswerter Unterstützung durch die Deutsche Forschungs-Gemein-schaft. 相似文献
13.
The effect of an asymmetrical skeleton photoperiod scheduleee was studied on the gonadal development in a tropical finch, the weaver bird (Ploceus philippinus). The schedul comprised a short nonstimulatory primary photoperiod of 6 hrs and a secondary much shorter lightperiod given as a 15-min light pulse at different times in the dark period. The light pulse 11 hrs after the basic period resulted in gonadal stimulation, while light pulse in contimuation with the basic period or 8, 14 or 21 hrs after the basicperiod was not stimulatory. The "photoinducible phase" was much more precisely outlined by shifting the birds from an 8-h to a 10-h pulse, and from an 11-h to 12-h pulse and was found to be very short, lasting about an hour falling between 11 and 12 hrs after the primary light period. The short photoinducible phase may be of an adaptive value since in the tropics the difference between the shortest and the longest daylength is also rather small (3 hrs and 15 min at Varanasi, 25degrees N). Clearly the weaver bird possesses a fine time-measuring device involving an endogenous circadian rhythm in photosensitivity. In nature, spermatogenesis in this bird also begins in March when the daylength exceeds 11 hrs (thus perhaps coinciding with the photoinducible phase). In rather small, photoperiod may not serve as a cue to trigger seasonal reproductive periodicity, it seems that photoperiod can act as a Zeitgeber for the initiation of spermatogenesis in the weaver bird at least. 相似文献
14.
Circadian rhythms in stomatal opening and photosynthesis had shorter free-running periods than circadian rhythms in leaflet movement in bean plants (Phaseolus vulgaris L.) transferred from 12-hr photoperiods to constant conditions. The rhythm in leaflet movement had a period close to 27 hr, whereas the rhythm in stomatal opening, measured as conductance to water vapor, had a period close to 24 hr. Photosynthesis, measured as net assimilation of CO2, also oscillated with a period close to 24 hr. The periods of these rhythms did not vary with increasing temperature, demonstrating temperature compensation of the controlling oscillators. The difference in free-running periods displayed by these rhythms is evidence that multiple oscillators with different intrinsic frequencies operate in bean plants. 相似文献
15.
M. Hau E. Gwinner 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1992,170(4):403-409
Summary We studied the potential zeitgeber qualities of periodic food availability on the circadian rhythms of locomotor and feeding activity of house sparrows. The birds were initially held in a LD-cycle of 12:12 h, with food restricted to the light phase. After transfer to constant dim light, the birds remained entrained by the restricted feeding schedule. Following an exposure to food ad libitum conditions, the rhythms could be re-synchronized by the feeding cycle. Shortening of the zeitgeber period to 23.5 h resulted in the loss of entrainment in most birds, whereas a longer zeitgeber period of 25 h re-entrained the rhythms of most birds. Although these results prove that periodic food availability can act as a zeitgeber for the circadian rhythms of house sparrows, several features of our data indicate that restricted feeding is only a weak zeitgeber. The pattern of feeding activity prior to the daily time of food access shown under some experimental conditions suggests that anticipation is due to a positive phase-angle difference of the birds' normal circadian system rather than being caused by a separate pacemaker. 相似文献
16.
The cercariae of Schistosoma haematobium showed a diurnal periodicity of emergence from Bulinus globosus in a twelve hour light/dark cycle. Peak emission occurred at 11.00 hrs with a smaller peak at 20.00 hrs, following the start of the period of darkness. In continuous illumination this second peak was not seen, indicating that only the morning peak is circadian in origin. The evening peak occurs in response to dark treatment and can be produced by periods of darkness ranging from eight seconds to one hour. The longer the period of dark treatment the longer the rise in output is maintained on return to light conditions. Subjection of snails to periods of dark treatment during the normal light period caused a reduction in the evening peak with the largest effect seen following the longest period of darkness. An increased output of cercariae was seen following fifteen minutes exposure to a range of light intensities, the largest increase occurring at 10,000 and 7000 lux and complete darkness. The rapidity of this reaction to variations in light intensity suggests that the cercariae of S. haematobium are showing emergence in response to shadows. 相似文献
17.
W. J. Van Der Steen 《Acta biotheoretica》1970,19(2):87-93
Conclusion The overt oviposition rhythm ofLymnaea stagnalis should be regarded as an endogenous one. Despite considerable deviations of its period from 24 hrs, entrainment by 24 hr. external periodicities is possible. Possibly, entrainment is effectuated via a second — covert — internal rhythm. The fact that similar rhythms have not been established before, is probably connected with the nature of the phenomena to which previous studies have generally been limited. 相似文献
18.
Three manic-depressives were studied longitudinally. Several times a day, the patients measured and recorded their mood, vigor, oral temperature, finger counting, blood pressure, pulse rate, and urine volume. Then the acrophases of their circadian rhythms were computed by a least-squares fit. These patients displayed rhythm phases that were grossly abnormal. Systematic acrophase changes over time supported the hypothesis that manic-depressives have circadian rhythms that free-run faster than one cycle per 24 hrs. Lithium appeared to slow these rhythms and help the environmental synchronizer force physiological functions to coordinate better with the usual 24-h environmental cycles. 相似文献
19.
20.
Wilhelm Nultsch 《Archives of microbiology》1977,112(2):179-185
1. The effects of carbon dioxide, oxygen and pH on the inversion intensity of phototaxis of Chlamydomonas reinhardtii have been investigated. 2. With decreasing with CO2 tension the inversion intensity is decreased. 3. The gassing with CO2 can be substituted by hydrogencarbonate only to a small extent (20%). On the other hand, the effect of decreasing CO2 tension can be prevented also only in part by adjusting the pH to about 6.5-7.0. Thus the effect of CO2 on the inversion intensity of phototaxis is obviously a composite of a true CO2 effect and an effect of the concomitant pH change. 4. Oxygen has only a slight effect. In presence of oxygen (air) the phototactic reaction values are somewhat lower than in its absence. 5. Under certain conditions circadian rhythms seem to be initiated by changing oxygen as well as CO2 tensions. 6. Based on these results some contradictory results of older investigations are discussed. 相似文献