共查询到20条相似文献,搜索用时 109 毫秒
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目的基于小型猪的生理特点及循环系统与人类极为相似的特征,通过药物对其造模,建立一种新型的脉诊动物教学模型并对其进行评价。方法选用中华实验用成年小型猪6只(体重20±1.6kg,选取心率〈100次/min)。采用戊巴比妥钠麻醉,定位取脉位置,切脉后,用NX-8型脉诊仪同步采取正常脉图、二导心电图,血流动力学参数,血流变学指标。耳大静脉心得安注射液及葡聚糖T-500静脉给药进行造模,观察造模后一般情况(心率、皮肤颜色)及指下脉形变化,同步描记脉图及二导心电图,涩变后记录数值:不同时间点的心率变化;主波高(HB)、升支斜率(Sup)、降支斜率(Sdown)、降中峡位置(即降中峡高,主波高HE/HB)、升支时间,脉波周期(TAB/TAG)、主波峰时间,脉波周期(TB/TAG);并采集造模后血流动力学及血流变学各项参数。结果6头小型猪均造模成功,指下感觉涩变明显;脉图变化显著;与造模前相比HB、Sup、Sdown、HE/HB、TAB/TAG、TB/TAG差异均有显著性(除SdownP〈0.05外,其余均P〈0.01)。血流动力学及血流变学相关参数差异也均有显著。讨论通过运用小型猪建立涩脉模型成功、方法可靠,小型猪应用于脉诊教学实验方法简便、损伤小、可重复运用,有充分的可行性,以此为基础可以建立多种脉诊模型,以补充脉诊实验教学中的空白。 相似文献
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分析了当前外科手术权限管理方法和存在的问题。作为心血管病专科医院,尝试引入外科术前危险评分,对手术进行分级,建立手术准入控制系统和手术医师档案系统,全面记录医师外科手术情况,为评价医师技术能力和科学地审核医师手术权限申请提供了量化依据。 相似文献
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K. Hausmann 《Protoplasma》1979,100(2):199-213
Summary The membranes of the pellicle of the ciliatePseudomicrothorax dubius are investigated using thin section electron microscopy and freeze-fracture replicas. The plasma membrane is covered by a surface coat and is connected to the outer alveolar membrane by short, sometimes branched, bridges. The inner alveolar membrane is coated on both sides. The epiplasm lies in intimate contact with the cytoplasmic surface of this membrane, and there is a corresponding deposit on the other surface. This deposit is regularly striated.The epiplasmic layer and the alveoli are interrupted at sites of cytotic activity,e.g., the attachment sites of trichocysts, the cytoproct, and the parasomal sacs. The striated deposit ends where the epiplasm ends, indicating a direct relationship between these two epimembranous layers.There is a deposit along the sides of the first part of the tip of the trichocysts, and in this region the trichocyst membrane is free of intramembranous particles.The membrane of the parasomal sacs has a coat on both surfaces. That on the extraplasmic surface is similar to the surface coat of the plasma membrane. The origin of the cytoplasmic coat is unknown. The cytotic activity of these sacs is indicated by their highly irregular profiles. 相似文献
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Summary The differentiation of the spermatid, especially in reference to the formation of the flagellum, and transformation of the shape of the nucleus was investigated in the domestic fowl.In the early stage of the spermatid, a prominent Golgi apparatus appears around the centrioles. The Golgi vesicles then surround the axial-filament complex which develops from the distal centriole. These vesicles fuse to form continuous membrane at the earliest stage of flagellar formation, and in the succeeding stage Golgi lamellae are attached to the plasma membrane of the developing flagellum. From these observations, it is assumed that Golgi apparatus may be a source of the membrane system of the flagellum.The microtubules distributed around the nucleus form the circular manchette. The anterior region of the nucleus with the manchette is cylindrical in shape and the posterior region without it remains irregular in shape. When the circular manchette has been completed, the whole nucleus acquires a slender cylindrical shape. The circular manchette then changes into the longitudinal manchette. The nuclei of spermatids without a longitudinal manchette are abnormal in shape. In view of these observations it is assumed that the nuclear shaping of the spermatid may be accomplished by circular manchette and the maintenance of shape of the elongated nucleus by longitudinal manchette.The authors wish to thank Mr. Takayuki Mori for his helpful suggestions and technical advices 相似文献
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Panova IG Sharova NP Dmitrieva SB Levin PP Tatikolov AS 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2008,151(4):676-681
This study aimed to analyze the aqueous humor (AH) and the vitreous body (VB) of the eye of the adult frog Rana temporaria L. as a representative species of amphibians, which lead a semi-terrestrial life. The presence of collagen, albumin, uric acid and electron donors was shown in both media; however, there are slight differences in their concentrations. To determine collagen, a spectral-fluorescent probe, cyanine dye, was used. The presence of collagen in AH of the frog was found at the first time. The total content of electron donors (ascorbic and uric acids, tryptophan, and tyrosine) in VB and HA was roughly estimated at ~ 1.5 × 10− 4 mol/L. Both VB and AH absorb light in similar UV regions. The total protein and albumin contents in AH were found to be somewhat higher than those in VB. The uric acid content was at an equally low level in both intraocular media. It is supposed that the similarity of VB and AH compositions shown in this work is due to some exchange between VB and AH contents in the course of accommodation. The role of intraocular fluids in physiological functions of the eye and in protecting the retina against UV light is discussed. 相似文献
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Summary The choriocapillaris is a fenestrated capillary bed located posterior to the retinal pigment epithelium. It serves as the main source of supply to the photoreceptors, retinal pigment epithelium, and other cells of the outer retina. The permeability of these capillaries to intravenously injected ferritin (MW — approx. 480,000; mol. diam. 11 nm) was examined in the mouse, rabbit, and guinea pig, each of which is characterized by a different type of retinal vascularization. In all three species, the bulk of the ferritin remained in the capillary lumina, where it appeared to be blocked at the level of the diaphragmed fenestrae. Some ferritin was present in endothelial cell vacuoles. The results confirm previous work on the rat choriocapillaris and indicate that the barrier function of the choriocapillary endothelium is present even among species in which the retinal circulation differs significantly.Supported by NIH grant EY03418 相似文献