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MicroRNA(MiRNA,miR)通过调节信使RNA(mRNA)的表达,广泛参与心血管系统细胞的增殖、迁移、分化、凋亡等病理生理过程,在心血管系统疾病的发生发展过程中起着重要的调控作用。越来越多的研究表明,针对缺血性心血管疾病的发病机制,通过特异性调节miRNA的活性,抑制相关蛋白的表达,对各种缺血性心脏病具有显著的治疗作用。但目前开发miRNA靶向治疗药物尚缺乏大规模的临床试验研究,其有效性和安全性需进一步证实。本文旨在综述MicroRNA在靶向治疗缺血性心脏病中的研究进展,以期为开发MiRNA靶向治疗药物治疗缺血性心脏病提供更多的理论依据。 相似文献
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方舟 《上海生物医学工程》2003,24(1):56-57
20多年来,介入心脏学得到了快速发展。据统计,美国每年冠心病介入手术量大约为100万例,我国在2001年超过1.6万例。介入心脏病学发展的每一个阶段,都面临不同的挑战。在第十三届长城国际心脏病学会议上,美国佛罗里达大学医学院教授、国际老年心脏病学会主席Vlietstra将加入心脏病学的发展分为四个阶段,即诞生阶段、发展阶段、改进阶段、成熟阶段,对介入心脏病学的发展进行了系统的回顾。 相似文献
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目的 了解江苏省医疗质量安全事件发生概况及影响因素。方法 选取2011年5月—2014年12月江苏省医疗质量安全事件的信息数据进行实证研究,分析存在的问题。结果 (1)医疗质量安全事件高发地点主要为住院病房、门诊和急诊;(2)高发科室为骨科、普外科、呼吸内科、心血管内科以及妇产科。结论 完善报告制度,强化主动上报意识;丰富报告系统功能,提升统计分析水平;加强高危科室风险防范,提高医疗机构管理水平。 相似文献
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The parameters estimated from traditional A/C
i curve analysis are dependent upon some underlying assumptions that substomatal CO2 concentration (C
i) equals the chloroplast CO2 concentration (C
c) and the C
i value at which the A/C
i curve switches between Rubisco- and electron transport-limited portions of the curve (C
i-t) is set to a constant. However, the assumptions reduced the accuracy of parameter estimation significantly without taking
the influence of C
i-t value and mesophyll conductance (g
m) on parameters into account. Based on the analysis of Larix gmelinii’s A/C
i curves, it showed the C
i-t value varied significantly, ranging from 24 Pa to 72 Pa and averaging 38 Pa. t-test demonstrated there were significant differences in parameters respectively estimated from A/C
i and A/C
c curve analysis (p<0.01). Compared with the maximum ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation rate (Vcmax), the maximum electron transport rate (Jmax) and Jmax/Vcmax estimated from A/C
c curve analysis which considers the effects of g
m limit and simultaneously fits parameters with the whole A/C
c curve, mean Vcmax estimated from A/C
i curve analysis (Vcmax-C
i) was underestimated by 37.49%; mean Jmax estimated from A/C
i curve analysis (Jmax-C
i) was overestimated by 17.8% and (Jmax-C
i)/(Vcmax-C
i) was overestimated by 24.2%. However, there was a significant linear relationship between Vcmax estimated from A/C
i curve analysis and Vcmax estimated from A/C
c curve analysis, so was it Jmax (p<0.05). 相似文献
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Purified cytochrome from bovine adrenocortical mitochondria was incorporated into liposomes by the cholate-dilution method utilizing either dialysis or Sephadex gel filtration. Among synthetic phospholipids tested, dioleoylglycerophosphocholine showed the best stability during the incorporation of into liposomes. A maximum amount of heme was incorporated into liposomes at a molar ratio of phospholipid to the cytochrome of approx. 200. When was incorporated into the dioleoylglycerophosphocholine liposomes by the cholate-filtration method, the showed two major populations on the elution pattern of the Sepharose 4B gel filtration, and were seen at a diameter of 200–600 Å and its aggregated forms. When the cytochrome was incorporated into dioleoylglycerophosphocholine liposomes or cholesterol-free adrenocortical mitochondrial liposomes, was less stable than in aqueous solution. Cholesterol or adrenodoxin markedly stabilized the liposomal . Liposomal required cholesterol for its optimum reduction with adrenodoxin, adrenodoxin reductase, and NADPH in the presence of CO. About 70% of the total heme in the dioleoylglycerophosphocholine liposomes was reduced by the enzymatic reduction in the presence of cholesterol, indicating that 70% of the total molecules are exposed to the surface of the outer monolayer. In order to see the location of the heme in membrane, the dioleoylglycerophosphocholine-liposomal was subjected to treatment. This reagent destroyed the liposomal . These results suggest that the heme is located in the proximity of the reacting sites which are exposed to the surface, or located on the vincinity of polar heads of the membrane. 相似文献
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Tadashi Ogawa Mitsuru Fukuda Kei Sasaoka 《Biochimica et Biophysica Acta (BBA)/General Subjects》1973,297(1):60-69
One of the ninhydrin-negative alanine conjugates isolated from pea seedlings was identified as .The identification of this conjugate was carried out by a comparison of its gas-liquid chromatographic and mass spectrometric properties, and its nuclear magnetic resonance and infrared spectra with those of synthetic . The alanine in the conjugate was shown to be present as the D-isomer by enzymatic and chromatographic analyses. 相似文献
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Background
The evolution of alternatively spliced exons (ASEs) is of primary interest because these exons are suggested to be a major source of functional diversity of proteins. Many exon features have been suggested to affect the evolution of ASEs. However, previous studies have relied on the K A /K S ratio test without taking into consideration information sufficiency (i.e., exon length > 75 bp, cross-species divergence > 5%) of the studied exons, leading to potentially biased interpretations. Furthermore, which exon feature dominates the results of the K A /K S ratio test and whether multiple exon features have additive effects have remained unexplored. 相似文献15.
The ATP/ADP exchange is shown to be a partial reaction of the by the absence of measurable nucleoside diphosphokinase activity and the insensitivity of the reaction to , -di(adenosine-5′) pentaphosphate, a myokinase inhibitor. The exchange demonstrates an absolute requirement for Mg2+ and is optimal at an ADP/ATP ratio of 2. The high ATP concentration () required for maximal exchange is interpreted as evidence for the involvement of a low affinity form of nucleotide site. The ATP/ADP exchange is regarded as evidence for an ADP-sensitive form of the phosphoenzyme. In native enzyme, pre-steady state kinetics show that the formation of the phosphoenzyme is partially sensitive to ADP while modification of the enzyme by pretreatment with 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) in the absence of Mg2+ results in a steady-state phosphoenzyme population, a component of which is ADP sensitive. The ATP/ADP exchange reaction can be either stimulated or inhibited by the presence of K+ as a function of pH and Mg2+. 相似文献
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Flaviano S. Martins Aparecida A. Silva Angélica T. Vieira Flávio H. F. Barbosa Rosa M. E. Arantes Mauro M. Teixeira Jacques Robert Nicoli 《Archives of microbiology》2009,191(8):623-630
The present work investigates some probiotic properties of four different microorganisms (Bifidobacterium animalis var. lactis BB-12, Escherichia coli EMO, Lactobacillus casei and Saccharomyces boulardii). In vitro and in vivo tests were carried out to compare cell wall hydrophobicity, production of antagonistic substances,
survival capacity in the gastrointestinal tract of germ-free mice without pathological consequence, and immune modulation
by stimulation of Küpffer cells, intestinal sIgA and IL-10 levels. In vitro antagonism against pathogenic bacteria and yeast
was only observed for the probiotic bacteria B. animalis and L. casei. The hydrophobic property of the cell wall was higher for B. animalis and E. coli EMO, and this property could be responsible for a better ability to colonize the gastrointestinal tract of germ-free mice.
Higher levels of sIgA were observed mainly for S. boulardii, followed by E. coli EMO and B. animalis, and only S. boulardii induced a significant higher level of IL-10. In conclusion, for a probiotic use, S. boulardii presented better characteristics in terms of immunomodulation, and B. animalis and L. casei for antagonistic substance production. The knowledge of the different probiotic properties could be used to choice the better
microorganism depending on the therapeutic or prophylactic application. 相似文献
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P. K. Golovatenko-Abramov E. A. Zhirkova E. G. Kolokolchikova V. S. Bocharova E. S. Platonov 《Russian Journal of Developmental Biology》2010,41(4):240-246
One of the phenotypic effects of mutation in the Hr gene in mice is disintegration of hair follicles and their degeneration into open funnel-shaped structures (utricles) opened
on skin surface and cysts located in the depth of the dermis. The aim of the current study consists in analysis of the process
of reparative regeneration of skin in homozygotous mice with one of the mutant alleles of the Hr gene—Hr
hr
. It is shown that epithelial cells that constitute the inner pavement of cysts take part in the process of epithelization
of deep skin wounds. This indicates that the competence of ectodermal cells in relation to inductive signals from injured
skin remains in Hr
hr
homozygote mice, in spite of the significant anatomic abnormalities of the hair follicles. 相似文献
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Taras K. Antal 《Biophysical journal》2009,96(11):4672-4680
The oxygen-evolving complex of Photosystem II cycles through five oxidation states (S0-S4), and dark incubation leads to 25% S0 and 75% S1. This distribution cannot be reached with charge recombination reactions between the higher S states and the electron acceptor QB−. We measured flash-induced oxygen evolution to understand how S3 and S2 are converted to lower S states when the electron required to reduce the manganese cluster does not come from QB−. Thylakoid samples preconditioned to make the concentration of the S1 state 100% and to oxidize tyrosine YD were illuminated by one or two laser preflashes, and flash-induced oxygen evolution sequences were recorded at various time intervals after the preflashes. The distribution of the S states was calculated from the flash-induced oxygen evolution pattern using an extended Kok model. The results suggest that S2 and S3 are converted to lower S states via recombination from S2QB− and S3QB− and by a slow change of the state of oxygen-evolving complex from S3 and S2 to S1 and S0 in reactions with unspecified electron donors. The slow pathway appears to contain two-electron routes, S2QB → S0QB, and S3QB → S1QB. The two-electron reactions dominate in intact thylakoid preparations in the absence of chemical additives. The two-electron reaction was replaced by a one-electron-per-step pathway, S3QB → S2QB → S1QB in PS II-enriched membrane fragments and in thylakoids measured in the presence of artificial electron acceptors. A catalase effect suggested that H2O2 acts as an electron donor for the reaction S2QB → S0QB but added H2O2 did not enhance this reaction. 相似文献
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Showdomycin inhibited pig brain ( with pseudo first-order kinetics. The rate of inhibition by showdomycin was examined in the presence of 16 combinations of four ligands, i.e., Na+, K+, Mg2+ and ATP, and was found to depend on the ligands added. Combinations of ligands were divided into five groups in terms of the magnitude of the rate constant; in the order of decreasing rate constants these were: (1), (2) , , and none, (3) , and , (4) and , , , and ATP. The highest rate was obtained in the presence of Na+, Mg2+ and ATP. The apparent concentrations of Na+, Mg2+ and ATP for half-maximum stimulation of inhibition () were 3 mM, 0.13 mM and 4μM, respectively. The rate was unchanged upon further increase in Na+ concentration from 140 to 1000 mM. The rates of inhibition could be explained on the basis of the enzyme forms present, including E1, E2, ES, E1-P and E2-P, i.e., E2 has higher reactivity with showdomycin than E1, while E2-P has almost the same reactivity as E1-P. We conclude that the reaction of ( proceeds via at least four kinds of enzyme form (E1, E2, E1 · nucleotide and EP), which all have different conformations. 相似文献
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The partial purification of from pig lens has been achieved by treatment with deoxycholate followed by density gradient centrifugation. The specific activity of the final preparation, ranging from 300 to 500 nmol/h per mg protein, is increased approx. 100-fold compared to the homogenate. A parallel increase in activity is also observed. Sodium dodecyl sulfate (SDS) gel electrophoresis reveals six major protein bands, one of which is the 93 kDa α subunit of which can be phosphorylated by reaction with [γ-32P]ATP. A second band contains a glycoprotein which displays an apparent molecular weight of 51 000 and thus appears to be the β subunit of the enzyme. The enzyme is sensitive to ouabain with the for and inhibition being 1.2 and 1.3 μM, respectively. Several agents which inhibit from other tissues such as oligomycin, Ca2+, vanadate, , (PCMBS) and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) also inhibit the lens enzyme. Monovalent cations other than K+ are partially effective in activating the ()-ATPase and activities. The K+ congeners were relatively more effective in supporting compared to activity. Other kinetic properties of the lens enzyme are also comparable to those of the enzyme from other tissues. Utilizing the partially purified membrane bound enzyme, discontinuities in Arrhenius plots of activity, activity and fluoresence polarization of the fluidity probe, 1,6-diphenyl-1,3,5-hexatriene (DPH), are observed near the physiological temperature of lens. The possible significance of these observations for the mechanism of cataract formation are discussed. 相似文献