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1.
Plant growth regulating activity of dehydrocostus lactone possessing an α-methylene-γ-lactone moiety has been compared with its two derived C-16 lactones, in which a trisubstituted double bond and a cyclopropane ring are conjugated with the lactone carbonyl. The results show that the two latter compounds are slightly more active than dehydrocostus lactone. 相似文献
2.
The aim was to review knowledge about the interface betweenplant growth regulators and molecular checkpoints of the cell cycle. Atwhat level of biochemical regulation of the cell cycle do plant growthregulators interface? Are there different levels of interfacingdependent on the plant growth regulator involved? As a preamble totackling these questions, we overview the eukaryotic cell cycle withparticular emphasis on checkpoints that regulate the transition fromG0-G1-S-phase and G2-M. Cytokinins feature strongly as activators ofcell division in plants both in vivo and in vitro.Recent research has shown that zeatin treatment led to the up-regulationof CycD3 in Arabidopsis. This is a D-type cyclin showing stronghomology with vertebrate D cyclins which themselves are up-regulated byextracellular growth factors. Benzyladenine treatment can also shortenthe duration of S-phase through recruitment of latent origins of DNAreplication. Kinetin is involved in the phosphoregulation of the G2-Mcheckpoint; the major cyclin-dependent kinase (Cdk) at this checkpointhas recently been shown to be dephosphorylated as a result of cytokinintreatment, an effect which can also be mimicked by the fission yeastCdc25 phosphatase. Hence, a picture emerges of a cytokinin-inducedcontinuum of cell cycle activation through the up-regulation of a plantD-type cyclin at the G1 checkpoint and the phosphoregulation of the Cdkat the G2/M checkpoint. During S-phase, we argue for a link betweencytokinins and the proteins associated with replication origins.Gibberellic acid (GA) treatment induces internode elongation. Indeepwater rice, this response is mediated, at least partly, by aGA-induced up-regulation of a cyclin-Cdk at the G2-M checkpoint. Recentevidence has also linked abscisic acid to a cyclin-dependent kinaseinhibitor. These, so-called CKIs are negative regulators of Cdks whichfits with ABA's general role in growth inhibition; we await news ofethylene interactions. We highlight two instances of plant growthregulator-cell cycle interfacing during development, arguing for aninvolvement in microtubule orientation as a prerequisite to leafinitiation, and suggest a link between IAA and the activation of celldivisions in the pericycle required for lateral root initiation. A newD-type cyclin, recently discovered in Arabidopsis, may have akey role in this process. Finally, a model is presented which features ageneralised cyclin-Cdk checkpoint exhibiting various interfaces with theplant growth regulators. 相似文献
3.
A total of 96 samples of fresh fruits were collected to investigate the residues of selected plant growth regulators and fungicides. The analyses utilized ultra-high-performance liquid chromatography coupled with tandem mass spectrometry after a modified QuEChERS method. The results were evaluated according to maximum residue limits (MRLs) by the Chinese regulation. All blueberries, litchis, and watermelons were pesticide-free, and only one (1%) fruit sample contained pesticide residues above MRLs. Concerning the most frequently detected pesticide residues, carbendazim was found in 20% of the samples analyzed. Data obtained were then used to estimate the potential health risks associated with the exposures to these pesticides. The short-term risks were below 3.25% and the long-term risks were also very low, ranging from 0.0005% for dimethomorph to 0.4302% for prochloraz. 相似文献
4.
P. Katapodis A. Kavarnou S. Kintzios E. Pistola D. Kekos B.J. Macris P. Christakopoulos 《Biotechnology letters》2002,24(17):1413-1416
Neutral and acidic oligosaccharides were obtained from birchwood xylan by treatment with an endoxylanase, family 10 class, from Thermoascus aurantiacus. The main acidic xylooligosaccharide (aldotetrauronic acid) was separated from the hydrolysate by anion-exchange and size-exclusion chromatography and the primary structure was determined by 13C NMR spectroscopy. The aldotetrauronic yield was 15% (w/w) of the total solubilised sugars. The addition of purified aldoterauronic acid at 1.6–16 mg l–1 growth medium, induced callus and somatic embryogenesis in culture explants of common mallow (Malva silvestris L.) and cotton (Gosssypium hirsutum). 相似文献
5.
Plant growth regulators and the orchid cut-flower industry 总被引:1,自引:0,他引:1
Research involving plant growth regulators (PGRs) and orchids in areas of orchid growth and development are reviewed. For all areas covered in the review — seed germination and seedling growth, lateral shoot production, root production, flower initiation and development, postharvest physiology, and photosynthate partitioning — it was concluded that further studies would assist in clarifying potential uses for PGRs in the orchid cut-flower industry. It is suggested that extra PGR research on orchids is justified at the present time because of favourable prospects facing the orchid cut-flower industry. 相似文献
6.
7.
N.O. Jangaard 《Phytochemistry》1974,13(9):1769-1775
The in vitro and in vivo effects of a number of herbicides and plant growth regulators on phenylalanine ammonia-lyase (PAL) activity were investigated. The most elective in vitro inhibitors were product analogs, t-cinnamic and p-coumaric acids, and carbonyl reagents, hydroxylamine and nitromethane. Application of the herbicides diuron, dalapon, amiben, and chloropropham, to plants resulted in a decrease in the intracellular concn of PAL. The inhibitory effect of alachlor was found to be dose-responsive and somewhat specific. A correlation between PAL inhibition and herbicidal activity was observed for hydroxylamine. The cytokinin, pyranyl benzyladenine, (PBA) increased PAL activity in pigweed. The possibility of developing herbicides acting through PAL inhibition is discussed. 相似文献
8.
Effects of the growth regulators Barleyquat B, New 5c Cyocel, Cerone, Terpal and paclobutrazol on Rhizobium trifolii in vitro, on growth and root nodulation of clover grown in pots and on symbiotic nitrogen fixation were measured.Paclobutrazol in soil markedly reduced the weight of clover plants but had no effect on nodulation in relation to plant size. Paclobutrazol decreased the amount of nitrogen fixed per plant (measured by acetylene reduction) when present in soil at a concentration which would result from a single direct application at 0.25 kg a.i. ha–1 remaining unchanged and evenly distributed in the top 5 cm of soil. A concentration in soil equal to that from an application at 0.125 kg a.i. ha–1 had no significant effect on the rate of nitrogen fixation per plant and the rate relative to plants weight increased. It was evident that the inhibitory effect on plant growth was greater than on symbiotic nitrogen fixation. The other PGR's had virtually no effect on clover.None of the compounds seems likely to affect nitrogen fixation by a subsequent clover crop in the field if previously applied to a cereal crop once at typical rates of application.
Zusammenfassung Die Einflüsse der Wachstumsregulatoren Barleyquat B, New 5c, Cyocel, Cerone, Terpal und Paclobutrazol auf das Wachstum von Rhizobium trifolii in vitro, auf das Wachstum und Knöllchenbildung des Klees und auf die symbiotische N2-Bindung wurden bestimmt.Paclobutrazol im Boden führte zu einer bedeutenden Gewichtsverminderung der Kleepflanzen, blieb aber ohne Einfluß auf die Knöllchenbildung in bezug auf die Pflanzengröße. Paclobutrazol reduzierte die Menge Stickstoff gebunden pro Pflanze, (gemessen mittels Acetylenreduktion) wenn er im Boden in einer Konzentration enthalten war, die anwesend wäre, wenn eine einzige Applikation von 0,25 kg Wirkstoff.ha–1 unverändert und gleichmäßig in den oberen 5 cm des Bodens verteilt bleiben würde. Eine Bodenkonzentration gleich jener von einer Applikation von 0,125 kg Wirkstoff.ha–1 hatte keine signifikante Wirkung auf die N2-Bindungsleistung pro Pflanze, und das Bindungstempo in bezug auf das Pflanzengewicht nahm zu. Offensichtlich war die Hemmwirkung auf das Pflanzenwachstum größer als auf die symbiotische N2-Bindung.Wahrscheinlich wird keine der Verbindungen die N2-Fixierung des Klees im Freiland beeinträchtigen, wenn sie einmal vorher auf eine Getreidearte in der üblichen Aufwandmenge appliziert werden.相似文献
9.
植物生长调节剂的研究与应用 总被引:16,自引:0,他引:16
主要综述了植物生长调节剂的概况、植物生长调节剂的种类及其作用、植物生长调节剂的应用以及植物生长调节剂的安全使用原则,并展望了植物生长调节剂的应用前景。 相似文献
10.
The effects of auxins and cytokinin on callus formation, growth and regeneration of Gracilaria tenuistipitata Chang et Xia and G. perplexa Byrne et Zuccarello (Gracilariales, Rhodophyta) are reported. Plant growth regulators (PGR) in concentrations ranging from 0.1 to 100.0 μmol of indole‐3‐acetic acid, 2,4‐dichlorophenoxyacetic acid (2,4‐D), and kinetin (K) were added to the ASP 12‐NTA solid medium (0.7% agar), and apical and intercalary segments (5 mm long) were inoculated as initial explants. K stimulated growth rates of intercalary segments of G. tenuistipitata in a linear relation, and 2,4‐D (1.0 μmol) and K (10.0 μmol) stimulated growth rates of apical and intercalary segments of G. perplexa, respectively. The simultaneous formation of apical, basal, and intermediate calluses is reported for the first time in axenic tissue cultures of red algae. With intercalary segments of G. tenuistipitata, basal callus induction rates were higher than those of apical and intermediate calluses in the majority of treatments, and auxins had stimulatory effects on the formation of all callus types. In apical segments of G. perplexa, intermediate callus formation was stimulated only by treatment with 1.0 μmol of K, while apical callus formation was stimulated by indole‐3‐acetic acid (1.0–10.0 μmol), 2,4‐D (10.0–100.0 μmol), or K (0.1 μmol). Intercalary segments of G. perplexa developed only intermediate calluses, and the majority of treatments with PGR stimulated higher rates than those presented by apical segments. Potential for regeneration (development of adventitious plantlets originated from callus cells) was higher in apical calluses than in basal and intermediate calluses developed in intercalary segments of G. tenuistipitata. Moreover, auxins and cytokinin were essential to the induction of regeneration in intermediate calluses, while specific concentrations stimulated regeneration from basal and apical calluses. Plant regeneration in G. perplexa was observed only after transferring calluses from solid to liquid medium, and the majority of treatments with PGR had stimulatory effects. Regenerating plants of G. perplexa developed tetrasporangia, and released tetraspores giving rise to adult gametophytes. Our results indicate that auxins and cytokinin have a regulatory role in the growth and morphogenesis in G. tenuistipitata and G. perplexa, and diversity of responses presented by both species is related to specific developmental systems. 相似文献
11.
NADP-isocitrate dehydrogenase (NADP-ICDH) activity of radish (Raphanus sativus L.) seedlings pretreated with various plant growth regulators: KiN, GA, and ABA and different nitrogen sources viz. KNO3, NH4Cl and NH4NO3 in light and dark was investigated. ICDH activity was significantly higher in light than in dark; addition of different nitrogen
sources reduced it to a greater extent in NO3 supplementation. Among hormonal treatment only KiN showed slight promotion with KNO3 and NH4NO3. On the other hand in light KNO3 and/or NH4NO3 promoted ICDH activity and among hormones, KiN significantly promoted the activity in KNO3 and NH4NO3 supplemented seedlings while ABA was effective in NH4CL. It is suggested that in non-photosynthetic tissues, NADP-ICDH provides both reductant and carbon skeleton for glutamate
synthesis. 相似文献
12.
AIMS: To investigate the ability of Azospirillum sp., a facultative endophitic diazotrophic bacterium, to release plant growth regulators (PGR) such as polyamines, ethylene, indoleacetic acid and amino acids in both combined-N and N-free cultures. METHODS AND RESULTS: The presence of those substances was analysed by HPLC. Azospirillum sp. is capable of releasing PGR and amino acids into the culture medium. CONCLUSIONS: The type and quantity of the released substances varied, depending on the presence of combined-N in the medium. SIGNIFICANCE AND IMPACT OF THE STUDY: A better knowledge of PGR produced by Azospirillum sp. has been gained. 相似文献
13.
不同生长调节物质对水稻生长及镉积累的影响 总被引:3,自引:0,他引:3
比较脱落酸(ABA)、乙烯利(ETH)、水杨酸(SA)和茉莉酸甲酯(MeJ A)4种植物生长调节物质(PGR)对水稻生长及籽粒镉(Cd)积累的影响差异。试验采用重金属污染土种植水稻,于分蘖期、灌浆期各进行1次PGR叶面喷施处理,分析灌浆期叶片光合指标,丙二醛(MDA)含量以及收获期各部位生物量和Cd含量。结果表明:(1)低浓度ABA(5mg/L)可维持水稻正常产量;高浓度ABA(15mg/L)则导致产量下降。ETH对水稻地上部生长和单株产量有显著抑制作用,SA和MeJ A(0.56mg/L)均可保证地上部正常生长,维持正常产量。(2)外施4种PGR均抑制灌浆期叶片气孔开放,降低蒸腾速率和光合速率,抑制效果最明显的是高浓度MeJ A(0.56mg/L)。(3)在供试浓度范围内SA、低浓度ABA(5mg/L)以及高浓度MeJ A均可降低灌浆期叶片MDA含量,减少质膜过氧化水平。(4)4种PGR均可降低水稻籽粒Cd含量,其中低浓度ABA(5mg/L)抑制籽粒Cd积累的效应最佳。相关性分析结果表明,PGR抑制籽粒积累Cd的效应与地上部向籽粒转运Cd的调控机制有关,与蒸腾速率无显著相关关系。(5)综上所述,低浓度ABA(5mg/L)处理对水稻产量无影响,且籽粒Cd含量降低程度最大。适当浓度的PGR可降低水稻籽粒Cd含量,在中低度重金属污染农田生态修复实践中具有一定的应用前景,但必须精确控制PGR的处理时间和处理浓度,避免出现抑制生长和降低产量的负效应。 相似文献
14.
为了研究内生真菌发酵提取物和植物生长调节剂对大豆根际细菌多样性的影响,采用PCR-DGGE技术分析了各处理中不同发育期的大豆根际细菌群落变化。结果发现发酵提取液和植物生长调节剂能增加部分优势菌群的数量,但对根际细菌类群结构影响并不明显;生育周期也是影响根际细菌数量的重要因素。另外割胶测序发现优势菌群主要是Proteobacteria(变形菌门)、Acidobacteria(酸杆菌纲)、Nitrospira(硝化螺旋菌属)、Bradyrhizobium(慢生根瘤菌属)等,这些也都是大豆根际比较常见的细菌类群。 相似文献
15.
Pilar Garcia-Jimenez Marta Rodrigo Rafael R. Robaina 《Journal of applied phycology》1998,10(1):95-100
The influence of the plant growth regulators 2,4-D, GA3, BA and kinetin, and the polyamines putrescine, spermidine and spermine were tested on axenic in vitro cultures of carposporelings
of Grateloupia doryphora. The auxin 2,4-D (10-3 M) and the polyamine spermine (10-6 M and 10-3 M) induced a callus (disorganised cell mass that arose from the organised tissue of the carposporeling, as demonstrated by
microscopic monitoring of the tissue). Putrescine and spermidine (10-3 M) transformed the carposporelings into cell masses that produced shoots. BA (10-3 M) and kinetin (10-6 M and 10-3 M) were inhibitory. In 10-1 M glycerol-containing culture medium, which is known to induce the formation of morphogenic cell masses, the addition of
GA3 M) resulted in the inhibition of the morphogenesis (i.e. shoot emission) in the cell mass. The kinetin at 10-6 M inhibited morphogenesis, whilst at 10-3 M inhibited even the formation of the cell masses. The combination of glycerol (10-1 M) and the auxin 2,4-D (10-6 and 10-3 M) or the polyamines putrescine, spermidine and spermine (10-6 and 10-3 M) resulted in a bigger size of the cell masses that led to a higher amount of shoots per cell mass than in glycerol alone.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
16.
Nair S. Yokoya 《Phycological Research》2000,48(3):133-142
Axenic cultures of Gracilariopsis tenuifrons (Bird et Oliveira) Fredericq et Hommersand (Gracilariales, Rhodophyta) were established in ASP12‐NTA solid medium (0.4% agar and 1.0% sucrose) supplemented with plant growth regulators to evaluate the effects on apical callus formation and plant regeneration. Indole‐3‐acetic acid (IAA), 2,4‐dichlorophenoxyacetic acid (2,4‐D) and 6‐benzylaminopurine (BA) were added individually or in combinations (IAA : BA) over a range of concentrations from 0.5 to 5 mg L?1. Growth of apical and intercalary segments was stimulated by high concentrations of 2,4‐D (5 mg L?1) and a high IAA to BA ratio (IAA : BA = 5:1 mg L?1) respectively. Apical calluses were originated from divisions of apical and cortical cells located at apical regions of thallus segments and lateral branches. Low concentration of IAA (0.5 mg L?1) or a high IAA to BA ratio (IAA : BA = 5:1 mg L?1) were the optimal treatments for inducing apical callus formation in apical segments, while high concentration of IAA (5 mg L?1) stimulated the highest callus induction rate in intercalary segments. Conversely, equal parts IAA and BA (IAA : BA = 1:1 mg L?1) and low concentration of 2,4‐D (0.5 mg L?1) stimulated growth of apical calluses from apical and intercalary segments, respectively. Two processes of regeneration were observed: direct regeneration (upright axis originated from cells of proximal region of intercalary segments) and indirect regeneration (adventitious plantlet originated from cells of apical calluses). Direct regeneration was promoted significantly by treatment with a low IAA to BA ratio (IAA : BA= 1:5 mg L?1), and treatments with IAA (0.5 mgL?1) or 2,4‐D (0.5 or 5 mg L?1) significantly stimulated the elongation of upright axis. Plant growth regulators are essential to inducing indirect regeneration, and a high concentration of IAA (5 mg L?1) and BA (5 mg L?1) were the optimal treatments for inducing the regeneration of plantlets from apical calluses in apical and intercalary segments, respectively. Regenerating plantlets grew into plants morphologically similar to those formed from germinating spores, and became fertile after 6 weeks. The results suggest that auxins and cytokinins are involved in developmental regulatory processes in G. tenuifrons. The regeneration process from calluses in species of Gracilariales was observed for the first time in the present study. The culture system described for G. tenuifrons could be useful for micropropagation and for biotechnological applications in agarophytic algae. 相似文献
17.
C. Kevers M. Filali G. Petit-Paly D. Hagège M. Rideau Th. Gaspar 《In vitro cellular & developmental biology. Plant》1996,32(3):204-209
Summary Fully habituated organogenic and nonorganogenic sugarbeet calluses reacted to application of the synthetic auxin [3-benzo(b)
selenienyl] acetic acid by changes in growth and ethylene production. Treatment of fully habituated cells of periwinkle with
2,4-dichlorophenoxyacetic acid led to the decrease of free cytokinin contents (isopentenyl adenine, zeatin riboside, and zeatin)
during the late exponential phase of growth. The polyamine contents were also modified and the capacity to biotransform secologanin
into ajmalicine was decreased. Treatment of the habituated periwinkle cells with zeatin greatly increased the amount of a
polypeptide of 16 kDa; this response was more marked than that displayed by the auxin-dependent line. These data show that
hormone-independent calluses and cell suspensions can retain some sensitivity to growth hormones. However, differences of
responses were observed between the auxin-dependent lines and the habituated lines. 相似文献
18.
Julieta Muñoz Armando C. Cahue-López Rodrigo Patiño Daniel Robledo 《Journal of applied phycology》2006,18(2):209-218
The effects of plant growth regulators on callus induction rate and regeneration of K. alvarezii explants was evaluated. K. alvarezii calluses were induced in vitro with kinetin (K), 6-benzylaminopurine (B), 1-naphtalene acetic acid (N) and spermine (S). After 30 days, K. alvarezii explants produced filamentous calluses and isolated crystalline filaments growing from the medullar region and from cortical cells at the cut edge. The plant growth regulators 1-naphtalene acetic acid (1 mg L−1) and 6-benzylaminopurine (1 mg L−1) and the 1-naphtalene acetic acid + kinetin + spermine (1, 1, 0.018 mg L−1 respectively) combination produced 85 to 129% more calluses, with significant differences versus the control (p<0.05). Spermine at 0.018 mg L−1 produced calluses in the apical, intercalary and basal regions of explants. Spermine also reduced callus induction time to 7 days, which is faster than previously reported induction times with other plant growth regulators. An airlift bioreactor was designed and characterized to micropropagate K. alvarezii calluses. The bioreactor had mixing times ranging from 4.6–10.3 s at T
90 and T
95, which is shorter than those for the Fernbach (5.2–13.4 s) and balloon flasks (6.3–17.3 s). Mixing time standard deviations were smaller for the bioreactor (1.1–4.6) than for the Fernbach (9.3–13.6) and balloon flasks (5.5–15.8), suggesting an adequate flow regime within the bioreactor. The results are useful for improving callus induction in K. alvarezii and propagating microplantlets in an airlift bioreactor, and provide baseline data for macroalgal bioreactor culture. 相似文献
19.
Sridevy Sriskandarajah Ivan R. Kennedy Daiguan Yu Yao-Tseng Tchan 《Plant and Soil》1993,153(2):165-178
Treatment of wheat seedlings with the synthetic auxin, 2,4-dichlorophenoxyacetic acid (2,4-d), induced nodule-like structures or tumours (termed para-nodules) where lateral roots would normally emerge. The formation of these structures promoted increased rates of acetylene reduction at reduced oxygen pressure (0.02–0.04 atm) in seedling inoculated with Azospirillum brasilense, compared to seedlings inoculated without auxin treatment. Fluorescent microscopy, laser scanning confocal microscopy and direct bacterial counts all showed that the 2,4-d treatment stimulated internal colonization of the root system with azospirilla, particularly in the basal region of the nodular structures. Both colonization with azospirilla and acetylene-reducing activity were further stimulated by simultaneous treatment with another synthetic auxin, naphthaleneacetic acid (NAA) and, less reliably, with indoleacetic acid (IAA) and indolebutyric acid (IBA). These auxins produced shortening of many initiated lateral roots, although 20 times the concentration of NAA was required to achieve rounded structures similar to those obtained with 2,4-d. Treatment with NAA, IAA or IBA alone also stimulated colonization with azospirilla and acetylene reduction rates at 0.02 atm oxygen, but less effectively than by treatment with 2,4-d. Such exogenous treatments of wheat seedlings with synthetic growth regulators provide an effective laboratory model for studies on the development of a N2-fixing system in cereals. 相似文献
20.
Plant growth regulators and virus infection: A critical review 总被引:6,自引:0,他引:6
Virus infection can severely inhibit plant growth and distort development. This article reviews changes in plant growth regulator metabolism caused by infection. In general, virus infection decreases auxin and gibberellin concentrations and increases abscisic acid concentration. Ethylene production is stimulated in necrotic or chlorotic reactions to infection, but not where the virus spreads systemically without necrosis. While these broad trends are true for most host-virus combinations studied, several situations are recorded where the virus had other effects on growth substance concentration. Cytokinin changes do not show any common pattern: both increases and decreases after infection have been reported.The extent to which virus-induced changes in growth substance concentration could be responsible for observed alterations in host growth and development is discussed. While changes in abscisic acid, gibberellin and ethylene production seem potentially important, the experimental evidence does not provide conclusive proof for control of growth by these changes.The numerous investigations of effects of exogenous regulators on virus multiplication and pathogenesis are reviewed. Different regulators, or the same regulator applied at different times or concentrations, had very diverse effects, and in some cases did significantly alter virus multiplication and pathogenesis. However, such studies seem to have yielded disappointingly little understanding of the biochemistry of the host-virus interaction, and the possible involvement of growth substances in this.Possible uses of plant growth regulators in chemotherapy of virus disease, and their possible involvement in natural or induced resistance mechanisms are discussed. 相似文献