未来食品的低碳生物制造

受到人口增长过快、社会经济发展水平不平衡、人口老龄化和不健康饮食方式等影响,人类面临着食品和营养缺乏、部分人群中营养相关疾病高发等问题。同时,社会低碳发展的需求呼唤一种可持续的食物供给模式。因此,既能满足消费者口感和营养需求,又是绿色可持续食物供给模式的技术,例如功能糖、人造肉等未来食品技术,受到了广泛的关注。近年,新兴的生物制造技术及产品得到了迅猛发展,将会支撑形成绿色、低碳的未来食品产业,引发传统生产模式的深刻变革,是新兴生物经济的重大战略发展方向。本文聚焦于未来食品——功能糖、微生物蛋白及人造肉等关键辅配料的生物制造技术研究,追踪其在细胞工厂构建、工业环境下菌种测试与过程优化和衍生产品开发等研究的最新进展,展望未来的发展趋势,旨在为微生物制造未来食品的产业发展提供指导。     

Potential applications and emerging trends of species of the genus Dietzia: a review

Interest in attractive biological sources with multicriteria applications has been increasing during recent years. This study scrutinized the applications of Dietzia bacteria for future prospects. Apart from such present and well-established applications—as in therapeutic biotreatments for adult paratuberculosis animals, production of carotenoid pigments, and animal feed additives—their uses in biosurfactants and biodemulsifiers production, the pollutants bioremediation, biodegradation of petroleum hydrocarbons and crude oil and also production of extracellular polymeric substances (EPSs) have been exploited. The use of these bacteria as a biotechnological tool may lead to improve the optimization and quality assurance of food ingredients and products, the capability of degradation and remediation of environmental pollutants, and the efficiency of bioconversion systems for energy recovery and bioprocessing of value-added products.     

新加坡农业生物技术年报（2020）

目前,新加坡国内还没有任何植物生物技术投入商业化生产。截至2020年10月,新加坡共有43种转基因（genetically engineered,GE）植物产品获准用作食品或食品成分。新加坡遗传改良咨询委员会（Genetic Modification Advisory Committee,GMAC）最近修订了关于基因叠加事件的法规,采用“高覆盖低”的方法,对来源于先前GMAC认可的较低阶组合的高阶叠加事件组合豁免监管评估。     

Closing the phosphorus cycle in a food system: insights from a modelling exercise

Mineral phosphorus (P) used to fertilise crops is derived from phosphate rock, which is a finite resource. Preventing and recycling mineral P waste in the food system, therefore, are essential to sustain future food security and long-term availability of mineral P. The aim of our modelling exercise was to assess the potential of preventing and recycling P waste in a food system, in order to reduce the dependency on phosphate rock. To this end, we modelled a hypothetical food system designed to produce sufficient food for a fixed population with a minimum input requirement of mineral P. This model included representative crop and animal production systems, and was parameterised using data from the Netherlands. We assumed no import or export of feed and food. We furthermore assumed small P soil losses and no net P accumulation in soils, which is typical for northwest European conditions. We first assessed the minimum P requirement in a baseline situation, that is 42% of crop waste is recycled, and humans derived 60% of their dietary protein from animals (PA). Results showed that about 60% of the P waste in this food system resulted from wasting P in human excreta. We subsequently evaluated P input for alternative situations to assess the (combined) effect of: (1) preventing waste of crop and animal products, (2) fully recycling waste of crop products, (3) fully recycling waste of animal products and (4) fully recycling human excreta and industrial processing water. Recycling of human excreta showed most potential to reduce P waste from the food system, followed by prevention and finally recycling of agricultural waste. Fully recycling P could reduce mineral P input by 90%. Finally, for each situation, we studied the impact of consumption of PA in the human diet from 0% to 80%. The optimal amount of animal protein in the diet depended on whether P waste from animal products was prevented or fully recycled: if it was, then a small amount of animal protein in the human diet resulted in the most sustainable use of P; but if it was not, then the most sustainable use of P would result from a complete absence of animal protein in the human diet. Our results apply to our hypothetical situation. The principles included in our model however, also hold for food systems with, for example, different climatic and soil conditions, farming practices, representative types of crops and animals and population densities.     

传统与未来的碰撞：食品发酵工程技术与应用进展

随着生物技术的飞速发展,作为食品生物工程的主要组成部分,食品发酵工程技术不断升级,在传统发酵食品的菌种、发酵过程、产品品质得到改善的同时,生物制造的功能食品组分、未来食品等新型产品也应运而生。首先概述了由生物技术和信息技术的进步带来的食品发酵研究手段与生产方式的多层面变革,并重点阐释了利用食品合成生物学设计构建细胞工厂的思路和方法,以及食品生物工程在微生物分析、过程工程和分离工程方面的智能化进程。其次,介绍了现代食品生物工程技术在改善传统发酵食品品质及安全性、生产功能食品组分、添加剂和酶制剂、创制未来食品和开发新型益生食品方面的应用进展。最后,对全球和我国食品发酵产业面临的挑战和未来发展趋势进行了总结和展望,以期为食品发酵的技术革新和工业化应用提供参考。     

Risk Associated with the Use of Selected Ingredients in Food Supplements

This review focuses on four new product categories of food supplements: pre-workout, fat burner/thermogenic, brain/cognitive booster, and hormone/testosterone booster. Many food supplements have been shown to be contaminated with unauthorized substances. In some cases, the ingredients in the new categories of dietary supplements were medicinal products or new synthetic compounds added without performing clinical trials. Some of the new ingredients in dietary supplements are plant materials that are registered in the pharmacopoeia as herbal medicines. In other cases, dietary supplements may contain plant materials that have no history of human use and are often used as materials to ‘camouflage’ stimulants. In the European Union, new ingredients of dietary supplements, according to European Food Safety Authority or unauthorized novel food. Furthermore, selected ingredients in dietary supplements may be prohibited in sports and are recognized as doping agents by World Anti-Doping Agency.     

An array of possibilities for pectin

Pectins are a major component of plant cell walls and have numerous roles in plant growth and development. Extracted pectins are widely used as functional food ingredients in products including ice creams, jams, jellies and milk drinks. Although all are based on a galacturonan-rich backbone, pectins are an immensely diverse family of polysaccharides, the functional properties of which are dictated by their fine structures. Understanding the biological roles of pectins and optimizing their industrial usage requires a detailed knowledge of their diversity and spatial and temporal distributions, and microarray technology is a promising tool for high throughput pectin analysis. This article discusses the technical aspects of the production of pectin microarrays and explores their current and potential future uses in the context of recent work in the field.     

Current Prospects on Production of Microbial Lipid and Other Value-Added Products Using Crude Glycerol Obtained from Biodiesel Industries

Production of microbial lipids using crude glycerol from the biodiesel industry is reviewed in this paper. Approximately 10 wt.% of crude glycerol is obtained for every batch of biodiesel. The crude glycerol accumulated contains various impurities and hence cannot be used for any commercial applications without further purification. Its conversion via biological and chemical routes into valuable products has been studied by different researchers. Varieties of fungal, yeasts, and algal species have been used to produce microbial lipids from crude glycerol. However, research focus on screening a robust industrial oleaginous strain capable of doing this is still on-going. Due to its chemical similarity to vegetable oils, microbial lipids are considered a potential renewable feedstock for biodiesel production and for applications in food and pharmaceutical industries. Its conversion to polyols and subsequently to biobased polymers is also being explored. The rising price of vegetable oils, increasing energy demands, growing environmental concerns, and availability of crude glycerol as a cheap carbon substrate result in considerable potential for the application of these processes in the future.     

Pectin methylesterase inhibitor

Pectin methylesterase (PME) is the first enzyme acting on pectin, a major component of plant cell wall. PME action produces pectin with different structural and functional properties, having an important role in plant physiology. Regulation of plant PME activity is obtained by the differential expression of several isoforms in different tissues and developmental stages and by subtle modifications of cell wall local pH. Inhibitory activities from various plant sources have also been reported. A proteinaceous inhibitor of PME (PMEI) has been purified from kiwi fruit. The kiwi PMEI is active against plant PMEs, forming a 1:1 non-covalent complex. The polypeptide chain comprises 152 amino acid residues and contains five Cys residues, four of which are connected by disulfide bridges, first to second and third to fourth. The sequence shows significant similarity with the N-terminal pro-peptides of plant PME, and with plant invertase inhibitors. In particular, the four Cys residues involved in disulfide bridges are conserved. On the basis of amino acid sequence similarity and Cys residues conservation, a large protein family including PMEI, invertase inhibitors and related proteins of unknown function has been identified. The presence of at least two sequences in the Arabidopsis genome having high similarity with kiwi PMEI suggests the ubiquitous presence of this inhibitor. PMEI has an interest in food industry as inhibitor of endogenous PME, responsible for phase separation and cloud loss in fruit juice manufacturing. Affinity chromatography on resin-bound PMEI can also be used to concentrate and detect residual PME activity in fruit and vegetable products.     

Bioconversions of maize residues to value-added coproducts using yeast-like fungi

Agricultural residues are abundant potential feedstocks for bioconversions to industrial fuels and chemicals. Every bushel of maize (approximately 25 kg) processed for sweeteners, oil, or ethanol generates nearly 7 kg of protein- and fiber-rich residues. Currently these materials are sold for very low returns as animal feed ingredients. Yeast-like fungi are promising biocatalysts for conversions of agricultural residues. Although corn fiber (pericarp) arabinoxylan is resistant to digestion by commercially available enzymes, a crude mixture of enzymes from the yeast-like fungus Aureobasidium partially saccharifies corn fiber without chemical pretreatment. Sugars derived from corn fiber can be converted to ethanol or other valuable products using a variety of naturally occurring or recombinant yeasts. Examples are presented of Pichia guilliermondii strains for the conversion of corn fiber hydrolysates to the alternative sweetener xylitol. Corn-based fuel ethanol production also generates enormous volumes of low-value stillage residues. These nutritionally rich materials are prospective substrates for numerous yeast fermentations. Strains of Aureobasidium and the red yeast Phaffia rhodozyma utilize stillage residues for production of the polysaccharide pullulan and the carotenoid astaxanthin, respectively.     

Application of Markov Chain Model to Calculate the Average Number of Times of Use of a Material in Society. An Allocation Methodology for Open-Loop Recycling. Part 1: Methodology Development (7 pp)

- Preamble. In this series of two papers, a methodology to calculate the average number of times a material is used in a society from cradle to grave is presented and applied to allocation of environmental impact of virgin material. Part 1 focuses on methodology development and shows how the methodology works with hypothetical examples of material flows. Part 2 presents case studies for steel recycling in Japan, in which the methodology is applied and allocation of environmental impact of virgin steel is conducted. - Abstract Goal, Scope and Background. It has been recognized that LCA has a limitation in assessing open cycle recycling of materials because of inevitable subjective judgments in setting system boundary. According with the enforcement of recycling laws, there has been a rapid increase in recycling ratio of materials at the end-of-life of products in many industrialized countries. So, materials' life cycle is getting more complicated, which makes it difficult to quantify the environmental impacts of materials used in a product in an appropriate way. The purpose of this paper is to develop a methodology to calculate the average number of times a material is used in a society from cradle to grave. The method developed in this paper derives the average number of times material is used; this value could be used for allocation of environmental burdens of virgin material as well as an indicator for assessing the state of material use in a certain year, based on material flow of material in that year. Main Features Our methodology is based on Markov chain model using matrix-based numerical analysis. A major feature of this method is that it creates transition probability matrices for a material from the way in which the material is produced, consumed, and recycled, making it possible to simply elicit indicators that assess the status of material use in products in society. Our methodology could be an alternative method to derive the average number of times material is used, which could be used for allocation of environmental burdens of virgin material. Results and Discussions The methodology was applied to hypothetical examples of material flows, in which a virgin material was produced and used in products, recycled and finally landfilled. In some cases, closed loop and open loop recycling of materials existed. The transition probability matrix was created for each material flow, and how many times a virgin material is used in products until all of the elements are ultimately landfilled. Conclusions This methodology is applicable to a complicated material flow if the status of residence of a material and its flow in a society can be figured out. All the necessary data are the amount of virgin material production, amount of the material used in products, recycling rate of the material at the end of life of each product, the amount of scrap of the material that are used for products. In Part 2 of this paper, case studies for steel were conducted.     

Proposal of a method for allocation in building-related environmental LCA based on economic parameters

Application and development of the LCA methodology to the context of the building sector makes several building specific considerations necessary, as some key characteristics of products in the building sector differ considerably from those of other industrial sectors. The largest difference is that the service life of a building can stretch over centuries, rather than decades or years as seen for consumer products. The result of the long service life is that it is difficult to obtain accurate data and to make relevant assumptions about future conditions regarding, for example, recycling. These problems have implications on the issue of allocation in the building sector, in the way that several allocation procedures ascribe environmental loads to users of recycled or reused products and materials in the future which are unknown today. The long service life for buildings, building materials and building components, is associated with the introduced concept of a virtual parallel time perspective proposed here, which basically substitutes historical and future processes and values with current data. Further, the production and refining of raw material as a parallel to upgrading of recycled material, normally contains several intermediate products. A suggestion is given for how to determine the comparability of intermediate materials. The suggested method for allocation presented is based on three basic assumptions: (1) If environmental loads are to be allocated to a succeeding product life cycle, the studied actual life cycle has to take responsibility for upgrading of the residual material into secondary resources. (2) Material characteristics and design of products are important factors to estimate the recyclable amount of the material. Therefore, a design factor is suggested using information for inherent material properties combined with information of the product context at the building level. (3) The quality reduction between the materials in two following product life cycles is indicated as the ratio between the market value for the material in the products. The presented method can be a good alternative for handling the problem of open-loop recycling allocation in the context of the building sector if a consensus for the use of the fictive parallel time perspective and the use of the design factor can be established. This as the use of the time perspective and design factor is crucial to be able to deal with the problem of long service lives for buildings and building materials and the specific characteristics of the same building materials and components built into different building contexts.     

Global Expanded Nutrient Supply (GENuS) Model: A New Method for Estimating the Global Dietary Supply of Nutrients

Insufficient data exist for accurate estimation of global nutrient supplies. Commonly used global datasets contain key weaknesses: 1) data with global coverage, such as the FAO food balance sheets, lack specific information about many individual foods and no information on micronutrient supplies nor heterogeneity among subnational populations, while 2) household surveys provide a closer approximation of consumption, but are often not nationally representative, do not commonly capture many foods consumed outside of the home, and only provide adequate information for a few select populations. Here, we attempt to improve upon these datasets by constructing a new model—the Global Expanded Nutrient Supply (GENuS) model—to estimate nutrient availabilities for 23 individual nutrients across 225 food categories for thirty-four age-sex groups in nearly all countries. Furthermore, the model provides historical trends in dietary nutritional supplies at the national level using data from 1961–2011. We determine supplies of edible food by expanding the food balance sheet data using FAO production and trade data to increase food supply estimates from 98 to 221 food groups, and then estimate the proportion of major cereals being processed to flours to increase to 225. Next, we estimate intake among twenty-six demographic groups (ages 20+, both sexes) in each country by using data taken from the Global Dietary Database, which uses nationally representative surveys to relate national averages of food consumption to individual age and sex-groups; for children and adolescents where GDD data does not yet exist, average calorie-adjusted amounts are assumed. Finally, we match food supplies with nutrient densities from regional food composition tables to estimate nutrient supplies, running Monte Carlo simulations to find the range of potential nutrient supplies provided by the diet. To validate our new method, we compare the GENuS estimates of nutrient supplies against independent estimates by the USDA for historical US nutrition and find very good agreement for 21 of 23 nutrients, though sodium and dietary fiber will require further improvement.     

四君茶与猴头菇健脾养胃的研究进展

四君茶与猴头菇含有丰富的营养物质与活性成分,是具有较高保健价值的药膳食疗原材料。在越来越提倡"治未病"及"回归自然"的背景下,药膳食疗产品的市场需求和潜力将会得到极大提升。本研究以健脾养胃为靶点,围绕其代表方四君茶与猴头菇的主要成分、药理作用、研究现状、发展前景作一综述,为今后相关药膳产品深入研发提供思路与依据。     

盐肤木生物质炼制工程

盐肤木为我国乡土植物,分布范围广,资源量丰富。盐肤木果实含有油脂,但含油量低,因此盐肤木是一种非典型油料植物,不能单独作为油料植物进行产业化开发。作者根据多年对盐肤木果实和枝桠梢头物料特性的研究,提出了以蒸汽爆破为核心技术的盐肤木生物质炼制新模式,提取的盐肤木果油申请了国家卫生部新资源食品并获得了新资源食品许可批文。以盐肤木资源为原材料,以蒸汽爆破技术为依托,集成不同组分分离技术,各组分分别进行功能转化,形成了盐肤木果油、生物柴油、蛋白饲料、黄酮、本色面巾纸、酚醛树脂、生物质成型燃料、沼气八大产品的盐肤木生物质炼制技术体系和生态产业链集成,为自然界中非单一经济型野生植物资源的开发提供了新的模式。     

Antibiotic residues in food animals: Public health concern

Antibiotics are used to treat disease and improve animal production. These antibiotics might result in deposition of residues in meat, milk and eggs which are not permitted in food intended for human consumption. This review report some health hazards of antibiotic residues in food. There are many factors influencing the occurrence of residues in animal products such as drug’s properties and their pharmacokinetic characteristics, physicochemical or biological processes of animals and their products. The use of antibiotics is necessary in the prevention and treatment of animal diseases. Moreover, these antibiotics also improve the performance of growth and feed efficiency, synchronizing the reproductive cycle and breeding performance. These may also lead to harmful residual effects. For this to be minimized, withdrawal periods must be observed. This withholding periods makes the residues to be negligible or no longer detected in foods. However, withdrawal period is established to safeguard human from exposure of antibiotics added food. Failure to respect this period, could result in one which produces potential threat to direct toxicity in human. Moreover, low levels of antibiotic exposure would result in alteration of microflora, causing disease and the possible development of resistant strains causing failure of antibiotic therapy. The regulation of these residues in food of animal origin is necessary to prevent the health of humans.     

Microbial Content of Nonsterile Therapeutic Agents Containing Natural or Seminatural Active Ingredients

The relationship was investigated between various chemical or pharmaceutical production processes and the extent of microbial contamination, of natural origin, of the resulting products. The products contained active ingredients of vegetable, enzymatic, or animal origin. It was concluded that (i) vegetable products practically free from microbes can be produced if the proper manufacturing steps are taken; (ii) sterilization of the media used to manufacture antibiotics, etc., produces products with little contamination; and (iii) products containing extracts of animal organs require careful refrigeration and addition of preservatives to produce acceptable levels of microbial contamination.     

Potential Applications of the Cyclic Peptide Enterocin AS-48 in the Preservation of Vegetable Foods and Beverages

Bacteriocins are antimicrobial peptides produced by bacteria. Among them, the enterococcal bacteriocin (enterocin) AS-48 stands for its peculiar characteristics and broad-spectrum antimicrobial activity. AS-48 belongs to the class of circular bacteriocins and has been studied in depth in several aspects: peptide structure, genetic determinants, and mode of action. Recently, a wealth of knowledge has accumulated on the antibacterial activity of this bacteriocin against foodborne pathogenic and spoilage bacteria in food systems, especially in vegetable foods and drinks. This work provides a general overview on the results from tests carried out with AS-48 in different vegetable food categories (such as fruit juices, ciders, sport and energy drinks, fresh fruits and vegetables, pre-cooked ready to eat foods, canned vegetables, and bakery products). Depending on the food substrate, the bacteriocin has been tested alone or as part of hurdle technology, in combination with physico-chemical treatments (such as mild heat treatments or high-intensity pulsed electric fields) and other antimicrobial substances (such as essential oils, phenolic compounds, and chemical preservatives). Since the work carried out on bacteriocins in preservation of vegetable foods and drinks is much more limited compared to meat and dairy products, the results reported for AS-48 may open new possibilities in the field of bacteriocin applications.