共查询到20条相似文献,搜索用时 46 毫秒
1.
2.
3.
4.
5.
6.
7.
C Kücherer H Lother R K?lling M A Schauzu W Messer 《Molecular & general genetics : MGG》1986,205(1):115-121
8.
9.
Autoregulation of the dnaA gene of Escherichia coli K12 总被引:31,自引:0,他引:31
Tove Atlung Erik S. Clausen Flemming G. Hansen 《Molecular & general genetics : MGG》1985,200(3):442-450
10.
11.
12.
13.
14.
15.
Initiation of Escherichia coli minichromosome replication at oriC and at protein n'' recognition sites. Two modes for initiating DNA synthesis in vitro. 总被引:6,自引:2,他引:4 下载免费PDF全文
The start sites for leading and lagging DNA strands were determined in vitro with minichromosomes as templates. Fragments from replication intermediates were analyzed by hybridization to single-stranded probes. Leading strand synthesis in the counterclockwise direction was found to originate in or close to (position 248 to -44) the minimal origin. Complementary lagging strand synthesis started several positions to the left outside of oriC. The results suggest in addition a concerted synthesis of leading and lagging strands following the dnaA directed assembly of initiation proteins at double-stranded oricC DNA (pre-replisome). In addition, DNA synthesis could initiate at protein n' recognition sequences located within and clockwise to the asnA gene. Initiation at n' sites was dependent on protein i activity, whereas leading and lagging strand initiation in the oriC region was not affected by protein i. Our results argue against an involvement of the phi X174-type primosome in the initiation of discontinuous DNA synthesis at oriC. An alternative function is suggested. 相似文献
16.
17.
18.
W Messer W Seufert C Schaefer A Gielow H Hartmann M Wende 《Biochimica et biophysica acta》1988,951(2-3):351-358
19.
20.