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Incorporation in vitro of labeled amino acids into proteins of rabbit reticulocytes 总被引:21,自引:0,他引:21
BORSOOK H DEASY CL HAAGENSMIT AJ KEIGHLEY G LOWY PH 《The Journal of biological chemistry》1952,196(2):669-694
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Chemical and biological diversity of protein structures and functions can be widely expanded by position-specific incorporation of non-natural amino acids carrying a variety of specialty side groups. After the pioneering works of Schultz's group and Chamberlin's group in 1989, noticeable progress has been made in expanding types of amino acids, in finding novel methods of tRNA aminoacylation and in extending genetic codes for directing the positions. Aminoacylation of tRNA with non-natural amino acids has been achieved by directed evolution of aminoacyl-tRNA synthetases or some ribozymes. Codons have been extended to include four-base codons or non-natural base pairs. Multiple incorporation of different non-natural amino acids has been achieved by the use of a different four-base codon for each tRNA. The combination of these novel techniques has opened the possibility of synthesising non-natural mutant proteins in living cells. 相似文献
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Incorporation of labelled amino acids into the enkephalins 总被引:6,自引:0,他引:6
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Incorporation of modified amino acids into proteins in vivo 总被引:4,自引:0,他引:4
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Summary Incorporation of three various amino acids ([3H]-tryptophan, [3H]-methionine or [3H]-leucine) into the non-histone chromatin proteins, synthesized in spleen cells of mice after immunization with IgG, is described.
Two new fractions of non-histone chromatin proteins (I-mol. wt. below 3 000 and B1-mol. wt. about 120 000), appearing during the immune reaction were labelled with [3H]-tryptophan and [3H]-methionine but not with [3H]-leucine. Synthesis of these fractions was observed only at the time of maximal RNA synthesis. A suggestion about the role
of tryptophan and methionine in non-histone chromatin proteins in the regulatory processes of gene activation is discussed
on the basis of their selective binding to DNA. 相似文献
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The role of microsomes in the incorporation of amino acids into proteins 总被引:20,自引:0,他引:20
KELLER EB ZAMECNIK PC LOFTFIELD RB 《The journal of histochemistry and cytochemistry》1954,2(5):378-386
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Shadia E. Ramadan A. A. Razak A. M. Ragab M. El-Meleigy 《Biological trace element research》1989,20(3):225-232
Aspergillus fumigatus, Aspergillus terreus, and Penicillium chrysogenum, a tellurium tolerant fungi, are able to grow on sulfur free medium amended with 0.2% (w/v) tellurite. Tellurium was incorporated into several types of low and high molecular weight proteins. The newly detected telluro-proteins contained an extraordinary high level of tellurium, as well as telluro-cysteine, telluro-cystine, telluro-methionine, and serine. 相似文献