共查询到20条相似文献,搜索用时 31 毫秒
1.
Pérez-Vich B Fernández-Martínez JM Grondona M Knapp SJ Berry ST 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):338-349
The genetic control of the synthesis of stearic acid (C18:0) and oleic acid (C18:1) in the seed oil of sunflower was studied
through candidate-gene and QTL analysis. Two F2 mapping populations were developed using the high C18:0 mutant CAS-3 crossed to either HA-89 (standard, high linoleic fatty
acid profile), or HAOL-9 (high C18:1 version of HA-89). A stearoyl-ACP desaturase locus (SAD17A), and an oleoyl-PC de-saturase
locus (OLD7) were found to cosegregate with the previously described Es1 and Ol genes controlling the high C18:0 and the high C18:1 traits, respectively. Using linkage maps constructed from AFLP and RFLP
markers, these loci mapped to LG1 (SAD17A) and to LG14 (OLD7) and were found to underlie the major QTLs affecting the concentrations
of C18:0 and C18:1, explaining around 80% and 56% of the phenotypic variance of these fatty acids, respectively. These QTLs
pleiotropically affected the levels of other primary fatty acids in the seed storage lipids. A minor QTL affecting both C18:0
and C18:1 levels was identified on LG8 in the HAOL-9×CAS-3 F2. This QTL showed a significant epistatic interaction for C18:1 with the QTL at the OLD7 locus, and was hypothesized to be
a modifier of Ol. Two additional minor C18:0 QTLs were also detected on LG7 and LG3 in the HA-89×CAS-3 and the HAOL-9×CAS-3 F2 populations, respectively. No association between a mapped FatB thioesterase locus and fatty acid concentration was found.
These results provide strong support about the role of fatty acid desaturase genes in determining fatty acid composition in
the seed oil of sunflower.
Received: 7 December 2000 / Accepted: 21 May 2001 相似文献
2.
Pérez-Vich Begoña Knapp Steven J. Leon Alberto J. Fernández-Martínez José M. Berry Simon T. 《Molecular breeding : new strategies in plant improvement》2004,13(4):313-322
Increased stearic acid (C18:0) content in the seed oil of sunflower would improve the oil quality for some edible uses. The sunflower line CAS-20 (C18:0 genotype Es1Es1es2es2), developed from the high C18:0 mutant line CAS-3 (C18:0 genotype es1es1es2es2; 25% C18:0), shows increased C18:0 levels in its seed oil (8.6%). The objective of this research was to map quantitative trait loci (QTL) conferring increased C18:0 content in CAS-20 in an F2 mapping population developed from crosses between HA-89 (wild type Es1Es1Es2Es2; low C18:0) and CAS-20, which segregates independently of the macromutation Es1 controlling high C18:0 content in CAS-3. Seed oil fatty acid composition was measured in the F2 population by gas-liquid chromatography. A genetic linkage map of 17 linkage groups (LGs) comprising 80 RFLP and 19 SSR marker loci from this population was used to identify QTL controlling fatty acid composition. Three QTL affecting C18:0 content were identified on LG3, LG11, and LG13, with all alleles for increased C18:0 content inherited from CAS-20. In total, these QTL explained 43.6% of the C18:0 phenotypic variation. Additionally, four candidate genes (two stearate desaturase genes, SAD6 and SAD17, and a FatA and a FatB thioesterase gene) were placed on the QTL map. On the basis of positional information, QTL on LG11 was suggested to be a SAD6 locus. The results presented show that increased C18:0 content in sunflower seed oil is not a simple trait, and the markers flanking these QTL constitute a powerful tool for plant breeding programs. 相似文献
3.
Elsa M. Vera-Ruiz Leonardo Velasco Alberto J. Leon Jose M. Fernández-Martínez Begoña Pérez-Vich 《Molecular breeding : new strategies in plant improvement》2006,17(3):291-296
Tocopherols are a family of fat soluble antioxidants of great value for both nutritional and technological properties of seed
oils. The four naturally occurring tocopherols (alpha-, beta-, gamma- and delta-tocopherol) widely differ for their relative
in vivo (vitamin E) and in vitro antioxidant properties. Sunflower (Helianthus annuus L.) seeds mainly contain alpha-tocopherol (95% of the total tocopherols), which has a great vitamin E value but a low in vitro activity. Conversely, beta-tocopherol shows more balanced in vitro and in vivo antioxidant properties, which is desired for specific uses of the oil. The sunflower line T589 is characterised by an increased
beta-tocopherol content in the seeds ( >30%), which is determined by the single gene Tph1. The objectives of this study were
to map the Tph1 gene by molecular markers (SSRs) and to develop a linkage map of the Tph1-encompassing region. High performance
liquid chromatography (HPLC) was used to phenotype 103 F2 and 67 F3 progeny from the mapping population CAS-12 × T589, which segregates for Tph1. Bulk segregant analysis identified two SSR
markers on linkage group (LG) 1 linked to Tph1. A large linkage group was constructed by genotyping additional SSRs and INDEL
markers. Tph1 mapped to the upper end of LG 1 and cosegregated with the SSR markers ORS1093, ORS222, and ORS598. The availability
of tightly linked PCR-based markers and the location of the Tph1 gene on the sunflower genetic map will be useful for marker-assisted
selection in sunflower and provides a basis for the physical mapping and positional cloning of this gene. 相似文献
4.
B. Pérez-Vich L. del Moral L. Velasco B. S. Bushman S. J. Knapp A. Leon J. M. Fernández-Martínez S. T. Berry 《Molecular breeding : new strategies in plant improvement》2016,36(4):43
Sunflower (Helianthus annuus L.) seed oil with high palmitic acid content has enhanced thermo-oxidative stability, which makes it well suited to high-temperature uses. CAS-5 is a sunflower mutant line that accumulates over 25 % palmitic acid in its seed oil, compared to 5–8 % in conventional cultivars. The objective of this study was to investigate the molecular basis of the high-palmitic acid trait in CAS-5 through both candidate gene and QTL mapping approaches. An F2 population derived from the cross between CAS-5 and the conventional line HA-89 was developed. A 3-ketoacyl-ACP synthase II (KASII) locus on a telomeric region of linkage group (LG) 9 of the sunflower genetic map was found to co-segregate with palmitic acid content in this population. The KASII locus explained the vast majority of the phenotypic variation (98 %) of the trait. Two minor QTL affecting palmitic acid content were also found on the lower half of LG 9 and on LG 17. Additionally, QTL associated with other major fatty acids (stearic, oleic, and linoleic acid) were identified on LG 1, 6, and 10. This result may reflect untapped genetic variation that could exist among sunflower cultivars for genes determining fatty acid composition. In addition to demonstrating the major role of a KASII locus in the accumulation of high levels of palmitic acid in CAS-5 seeds, this study stressed the importance of characterizing genes with minor effects on fatty acid profile in order to establish optimal breeding strategies for modifying fatty acid composition in sunflower seed oil. 相似文献
5.
B. Pérez-Vich R. Garcés J. M. Fernández-Martínez 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):105-111
Two sunflower (Helianthus annuus L.) mutants with high concentrations of saturated fatty acids in their seed oil have been identified and studied extensively.
The mutant line CAS-5 has high concentrations of palmitic acid (C16:0) (>25% compared with 7% in standard sunflower seed oil)
and low-C18:0 values (3%). CAS-3 is characterized by its high levels of stearic acid (C18:0) (>22% compared with 4% in standard
sunflower seed oil) and a low-C16:0 content (5%). CAS-5 also possesses elevated levels of palmitoleic acid (C16:1) (>5%),
which is absent in standard sunflower seed oil. The objective of this study was to determine the relationships between the
loci controlling the high-C16:0 and the high-C18:0 traits in these mutants. Plants of both mutants were reciprocally crossed.
Gas chromatographic analyses of fatty acids from the seed oil of F1, F2, F3 and the BC1F1 to CAS-5 generations indicated that the loci controlling the high-C16:0 trait exerted an epistatic effect over the loci responsible
for the high-C18:0 character. As a result, the phenotypic combination containing both the high-C16:0 levels of CAS-5 and the
high-C18:0 levels of CAS-3 was not possible. However, phenotypes with a saturated fatty acid content of 44% (34.5% C16:0+9.5%
C18:0) were identified in the F3 generation. These are the highest saturated (C16:0 and C18:0) levels reported so far in sunflower seed oil. When F3 C16:0 segregating generations in both a high- and a low-C18:0 background were compared, the high-C16:1 levels were not expressed
as expected in the high-C18:0 background (CAS-3 background). In this case, the C16:1 content decreased to values below 1.5%,
compared with >5% in a low-C18:0 background. As the stearoyl-ACP desaturase has been reported to catalyze the desaturation
from C16:0-ACP to C16:1-ACP, these results suggested that a decrease in its activity was involved in the accumulation of C18:0
in the high-C18:0 mutant CAS-3.
Received: 10 March 1999 / Accepted: 16 June 1999 相似文献
6.
《Plant Physiology and Biochemistry》2000,38(5):377-382
Three high stearic acid sunflower (Helianthus annuus L.) mutants, CAS-3, CAS-4 and CAS-8, accumulating 28, 15 and 14 % of stearic acid in the seed lipids have been biochemically characterised. In vivo conversion rate of palmitic acid into stearic acid is not altered in the mutants but the conversion rate of stearic acid into oleic acid shows a reduction that correlated with the total stearic acid content of seed lipid mutants. Two enzymatic activities are found to be involved in the mutant phenotype, the acyl-ACP thioesterase (EC 3.1.2.14) and the stearoyl-ACP desaturase (EC 1.12.99.6). Our data suggest that the high stearic phenotype is due to the combined effect of a reduced stearoyl-ACP desaturase activity and an acyl-ACP thioesterase with higher activity on stearoyl-ACP. The same thioesterase activity increment, found on stearoyl-ACP, was also found on palmitoyl-ACP, suggesting that the affected thioesterase activity could be a FatB type. 相似文献
7.
B. Pérez-Vich R. Garcés J. M. Fernández-Martínez 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):663-669
A sunflower mutant, CAS-3, with about 25% stearic acid (C18:0) in the seed oil was recently isolated after a chemical-mutagen
treatment of RDF-1-532 seeds (8% C18:0). To study the inheritance of the high C18:0 content, CAS-3 was reciprocally crossed
to RDF-1–532 and HA-89 (5% C18:0). Significant reciprocal-cross differences were found in one of the two crosses, indicating
possible maternal effects. In the CAS-3 and RDF-1–532 crosses, the segregation patterns of the F1, BC1, and F2 populations fitted a one-locus (designated Es1) model with two alleles (Es1, es1) and with partial dominance of low over high C18:0 content. Segregation patterns in the CAS-3 and HA-89 crosses indicated
the presence of a second independent locus (designated Es2) with two alleles (Es2, es2), also with partial dominance of low over high C18:0 content. From these results, the proposed genotypes (C18:0 content)
of each parent were as follows: CAS-3 (25.0% C18:0) =es1es1es2es2; RDF-1–532 (8.0% C18:0) =Es1Es1es2es2; and HA-89 (4.6% C18:0) =Es1Es1Es2Es2. The relationship between the proposed genotypes and their C18:0 content indicates that the Es1 locus has a greater effect on the C18:0 content than the Es2 locus. Apparently, the mutagenic treatment caused a mutation of Es1 to es1 in RDF-1–532.
Received: 20 September 1998 / Accepted: 1 February 1999 相似文献
8.
L. L. Qi T. J. Gulya B. S. Hulke B. A. Vick 《Molecular breeding : new strategies in plant improvement》2012,30(2):745-756
Sunflower rust, caused by the fungus Puccinia helianthi Schwein., was not a serious problem for many decades because of successful deployment of effective resistance genes in commercial sunflower (Helianthus annuus L.) hybrids in North America. In the 1980s and early 1990s, however, a shift in virulence of the rust race population in North America rendered most of the commercial hybrids susceptible to new virulent races. A germplasm line, HA-R2, carrying the rust resistance gene R 5 was released as a multi-race rust-resistant line in 1985 but has not been widely used in commercial hybrid production. R 5 remains effective against the prevalent rust races of sunflower in North America. This gene was previously reported to be associated with two simple sequence repeat (SSR) markers, ORS316 and ORS630, which were mapped to linkage group (LG) 13 of sunflower. However, out of the 63 markers of LG13 screened in the present study, only 18, including ORS316 and ORS630, were polymorphic. These markers, which covered all of LG 13, were assayed in 94 individual F2 progenies derived from the cross of HA 89 with HA-R2. All failed to detect any locus in LG13 associated with the gene R 5 . Subsequently, a bulked segregant analysis was employed with an additional 510 SSR markers selected from the remaining 16 LGs of the sunflower genome. This analysis demonstrated that the LG2 markers showed association with rust resistance. Genotyping of the 94 F2 individuals with 23 polymorphic SSR markers from LG2 confirmed the R 5 location on LG2, flanked by two SSR markers, ORS1197-2 and ORS653a, at 3.3 and 1.8?cM of genetic distance, respectively. The markers for R 5 developed in this study will provide a useful tool for speeding up deployment of the R 5 gene in commercial sunflower hybrid production. 相似文献
9.
Zhao Liu Sujatha Mulpuri Jiuhuan Feng Brady A. Vick Chao-Chien Jan 《Molecular breeding : new strategies in plant improvement》2012,29(2):275-284
The inheritance of a previously identified dominant Rf gene in the confection sunflower line RHA 280 has been determined and designated as Rf
3
. This study reports the mapping of the Rf
3
locus using an F2 population of 227 individuals derived from CMS HA 89-3149 × RHA 280. Bulked segregant analysis with 624
pairs of simple sequence repeat (SSR) primers and sequence tagged site (STS) primers identified two polymorphic SSR markers
each of linkage groups (LGs) 7 and 11 from a previous map. Results on 90 F2 individuals with 42 polymorphic markers of LGs
7 and 11 indicated that the Rf
3
gene was linked with eight markers on LG 7, including five SSR markers (ORS328, ORS331, ORS928, ORS966, and ORS1092) and
three expressed sequence tag (EST)-SSR markers (HT619-1, HT619-2, and HT1013). Further analysis of the total F2 population
of 227 individuals identified a co-dominant marker, ORS328, linked to Rf
3
at a genetic distance of 0.7 cM on one side, and a female-dominant marker HT1013 at 12.6 cM proximal to Rf
3
on the other side; a genetic distance of 47.1 cM for LG 7 was covered. This is the first report of an Rf gene from the confection sunflower. The closely linked marker to Rf
3
will facilitate marker-assisted selection, and provide a basis for cloning of this gene. 相似文献
10.
Sujatha Mulpuri Zhao Liu Jiuhuan Feng Thomas J. Gulya Chao-Chien Jan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(5):795-803
The inheritance of resistance to sunflower downy mildew (SDM) derived from HA-R5 conferring resistance to nine races of the
pathogen has been determined and the new source has been designated as Pl
13
. The F2 individuals and F3 families of the cross HA-R5 (resistant) × HA 821 (susceptible) were screened against the four predominant SDM races 300,
700, 730, and 770 in separate tests which indicated dominant control by a single locus or a cluster of tightly linked genes.
Bulked segregant analysis (BSA) was carried out on 116 F2 individuals with 500 SSR primer pairs that resulted in the identification of 10 SSR markers of linkage groups 1 (9 markers)
and 10 (1 marker) of the genetic map (Tang et al. in Theor Appl Genet 105:1124–1136, 2002) that distinguished the bulks. Of these, the SSR marker ORS 1008 of linkage group 10 was tightly linked (0.9 cM) to the Pl
13
gene. Genotyping the F2 population and linkage analysis with 20 polymorphic primer pairs located on linkage group 10 failed to show linkage of the
markers with downy mildew resistance and the ORS 1008 marker. Nevertheless, validation of polymorphic SSR markers of linkage
group 1 along with six RFLP-based STS markers of linkage group 12 of the RFLP map of Jan et al. (Theor Appl Genet 96:15–22,
1998) corresponding to linkage group 1 of the SSR map, mapped seven SSR markers (ORS 965-1, ORS 965-2, ORS 959, ORS 371, ORS 716,
and ORS 605) including ORS 1008 and one STS marker (STS10D6) to linkage group 1 covering a genetic distance of 65.0 cM. The
Pl
13
gene, as a different source with its location on linkage group 1, was flanked by ORS 1008 on one side at a distance of 0.9 cM
and ORS 965-1 on another side at a distance of 5.8 cM. These closely linked markers to the Pl
13
gene provide a valuable basis for marker-assisted selection in sunflower breeding programs. 相似文献
11.
12.
Li Gong Caifeng Li Ana Capatana Jiuhuan Feng Lili Qi Gerald J. Seiler Chao-Chien Jan 《Molecular breeding : new strategies in plant improvement》2014,34(1):159-166
The nuclear male sterility (NMS) trait is a useful tool for sunflower (Helianthus annuus L.) breeding and genetic programs. Previously, we induced NMS mutants in cultivated line HA 89. The mutants possessed single recessive genes ms 6, ms 7, and ms 8, respectively, in NMS HA 89-872, NMS HA 89-552, and NMS HA 89-747. Bulked segregant analysis based on the male-fertile and male-sterile DNA pools and 560 simple sequence repeat and insertion/deletion markers randomly selected from 17 linkage groups (LGs) were used to locate ms 6 to LG16, ms 7 to LG6, and ms 8 to LG5. Subsequent genotyping of three F2 populations of 88, 93, and 76 individuals confirmed their map positions. Additional polymorphic markers derived from four restriction fragment length polymorphism-converted sequence-tagged site primer pairs were identified. A partial linkage map consisting of eight markers was constructed for the ms 6 locus, covering a region of 69.24 cM, with markers ORS807 and ORS996 flanking the ms 6 locus at distances of 7.2 and 18.5 cM, respectively. Six markers were constructed for ms 7, covering a region of 53.4 cM, with ORS608 and ORS1229 flanking ms 7 at distances of 2.6 and 9.5 cM, respectively. Ten markers were constructed for ms 8, covering a region of 18.0 cM, with six markers below ms 8 and CRT518 above flanking ms 8 at distances of 7.4 and 3.8 cM, respectively. The markers and mapping information will be useful for selection of the recessive NMS genes in sunflower breeding programs. 相似文献
13.
Sunflower oil with high oleic acid content is in great demand due to its nutritional as well as industrial benefits. The trait is mainly controlled by dominant alleles at a major gene, Ol, with other modifiers. The objectives of this research were to map the oil content, oleic acid and linoleic acid content in sunflower seeds. An F2 mapping population from cytoplasmic male-sterile line COSF 7A (33–35 % oleic acid) and high oleic acid inbred line HO 5–13 (88–90 % oleic acid) was developed and phenotyped for oil content, oleic acid and linoleic acid content at the F2 seed level. High phenotypic and genotypic coefficients of variation were recorded for oleic acid and linoleic acid content. High heritability and high genetic advance as percent of mean was recorded for oleic acid and linoleic acid content. This indicated the presence of the additive type of gene action controlling the traits oleic acid content and linoleic acid content. The Ol gene was mapped to linkage group (LG) 14 and tightly linked to the marker HO_Fsp_b. In addition, two more quantitative trait loci (QTLs) for oleic acid content were identified in LG8 and LG9. Two QTLs for oil content and two QTLs for linoleic acid content were also identified. All these QTLs explained over 10 % of phenotypic variation. A study was conducted with 13 genotypes differing in oil quality as well as quantity over three seasons to assess the reliability of the identified QTLs over seasons. It resulted in the identification of two potential QTLs for oleic acid as well as linoleic acid content with the markers ORS 762 and HO_Fsp_b. These markers explained more than 57.6–66.6 % of phenotypic variation. Hence it can be concluded that these markers/QTLs would be useful in the marker-assisted selection breeding programme to improve oil quality. The present study also indicated the presence of at least two other genomic regions controlling oleic and linoleic acid content in sunflower. 相似文献
14.
The fatty acid compositions of half-seeds and whole seeds of the temperature-dependent high-stearic-acid sunflower (Helianthus annuus L.) mutant CAS-14 were unexpectedly different. We found that there is a longitudinal gradient starting from the embryo up to the end of the cotyledon. The stearic acid content varied from 9.7 to 34.6% in seeds produced in a growth chamber (39/24 degrees C; day/night), and from 14.0 to 34.4% in seeds produced in the field during the summer season (35-40 degrees C in daylight and 20-25 degrees C at night). The gradient occurs throughout seed formation, and is due to a spatial and non-temporal regulation of stearic acid desaturation. A similar temperature-regulated behaviour, but for oleic and linoleic acid contents, was found in normal sunflower seeds. Since the deposition of oil bodies was homogeneous during seed formation, seeds showed the gradient throughout their development. This non-homogeneous distribution must be due to differences in the enzymatic pathway of de-novo fatty acid desaturation along the seed, resembling a morphogen gradient. Other high-stearic-acid mutant lines, such as CAS-3, did not show any gradient. This is the first time that a gradient and an inheritable maternal control of the fatty acid composition have been found in oilseeds. 相似文献
15.
S. M. Rahman Y. Takagi T. Kinoshita 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):772-776
Stearic acid is one of the two saturated fatty acids found in soybean [Glycine max (L.) Merr.] oil, with its content in the seed oil of commercial cultivars averaging 4.0%. Two mutants, KK-2 and M25 with
two- and six-fold higher stearic acid contents in the seed oil than cv ‘Bay’, were identified after X-ray seed irradiation.
Our objective was to determine the genetic control of high stearic acid content in these mutants. Reciprocal crosses were
made between each mutant and ‘Bay’, and between the two mutants. No maternal effect for stearic acid content was observed
from the analysis of F1 seeds in any of the crosses. Low stearic acid content in ‘Bay’ was partially dominant to high stearic acid content in KK-2
and M25, and high stearic acid content in KK-2 was partially dominant to high stearic acid content in M25. Cytoplasmic effects
were not observed, as demonstrated by the lack of reciprocal cross differences for stearic acid content in our analysis of
F2 seeds from F1 plants. The stearic acid content in F2 seeds of KK-2בBay’ and M25בBay’ crosses segregated into three phenotypic classes which satisfactorily fit a 1:2:1 ratio,
indicating that high stearic acid content in KK-2 and M25 was controlled by recessive alleles at a single locus. The data
for stearic acid content in F2 seeds of the KK-2×M25 cross satisfactorily fit a 3:9:1:3 phenotypic ratio. The F2 segregation ratio and the segregation of F3 seeds from individual F2 plants indicated that KK-2 and M25 have different alleles at different loci for stearic acid content. The alleles in KK-2
and M25 have been designated as st
1 and st
2, respectively. The stearic acid content (>30.0%) found in the st
1
st
1
st
2
st
2 genotype is the highest known to date in soybean, but it was not possible to develop the line with this genotype because
the irregular seeds failed to grow into plants after germination. Therefore, tissue culture methods must be developed to perpetuate
this genotype.
Received: 28 March 1997 / Accepted: 18 April 1997 相似文献
16.
G. J. Ma Q. J. Song S. G. Markell L. L. Qi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(7):1423-1432
Key message
A novel rust resistance gene, R 15 , derived from the cultivated sunflower HA-R8 was assigned to linkage group 8 of the sunflower genome using a genotyping-by-sequencing approach. SNP markers closely linked to R 15 were identified, facilitating marker-assisted selection of resistance genes.Abstract
The rust virulence gene is co-evolving with the resistance gene in sunflower, leading to the emergence of new physiologic pathotypes. This presents a continuous threat to the sunflower crop necessitating the development of resistant sunflower hybrids providing a more efficient, durable, and environmentally friendly host plant resistance. The inbred line HA-R8 carries a gene conferring resistance to all known races of the rust pathogen in North America and can be used as a broad-spectrum resistance resource. Based on phenotypic assessments of 140 F2 individuals derived from a cross of HA 89 with HA-R8, rust resistance in the population was found to be conferred by a single dominant gene (R 15 ) originating from HA-R8. Genotypic analysis with the currently available SSR markers failed to find any association between rust resistance and any markers. Therefore, we used genotyping-by-sequencing (GBS) analysis to achieve better genomic coverage. The GBS data showed that R 15 was located at the top end of linkage group (LG) 8. Saturation with 71 previously mapped SNP markers selected within this region further showed that it was located in a resistance gene cluster on LG8, and mapped to a 1.0-cM region between three co-segregating SNP makers SFW01920, SFW00128, and SFW05824 as well as the NSA_008457 SNP marker. These closely linked markers will facilitate marker-assisted selection and breeding in sunflower.17.
Ming Zhang Zhao Liu Chao-Chien Jan 《Molecular breeding : new strategies in plant improvement》2016,36(3):32
Sunflower, the fifth largest oilseed crop in the world, plays an important role in human diets. Recently, sunflower production in North America has suffered serious yield losses from newly evolved races of sunflower rust (Puccinia helianthi Schwein.). The rust resistance gene, designated R 14 , in a germplasm line PH 3 originated from a wild Helianthus annuus L. population resistant to 11 rust races. PH 3 has seedling with an extraordinary purple hypocotyl color. The objectives of this study were to map both the R 14 rust resistance gene and the purple hypocotyl gene-designated PHC in PH 3, and to identify molecular markers for marker-assisted breeding for sunflower rust resistance. A set of 517 mapped SSR/InDel and four SNP markers was used to detect polymorphisms between the parents. Fourteen markers covering a genetic distance of 17.0 cM on linkage group (LG) 11 were linked to R 14 . R 14 was mapped to the middle of the LG, with a dominant SNP marker NSA_000064 as the closest marker at a distance of 0.7 cM, and another codominant marker ORS542 linked at 3.5 cM proximally. One dominant marker ZVG53 was linked on the distal side at 6.9 cM. The PHC gene was also linked to R 14 with a distance of 6.2 cM. Chi-squared analysis of the segregation ratios of R 14 , PHC, and ten linked markers indicated a deviation from an expected 1:2:1 or 3:1 ratio. The closely linked molecular or morphological markers could facilitate sunflower rust-resistant breeding and accelerate the development of rust-resistant hybrids. 相似文献
18.
Qi LL Hulke BS Vick BA Gulya TJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(2):351-358
Rust is a serious fungal disease in the sunflower growing areas worldwide with increasing importance in North America in recent
years. Several genes conferring resistance to rust have been identified in sunflower, but few of them have been genetically
mapped and linked to molecular markers. The rust resistance gene R
4
in the germplasm line HA-R3 was derived from an Argentinean open-pollinated variety and is still one of most effective genes.
The objectives of this study were to determine the chromosome location of the R
4
gene and the allelic relationship of R
4
with the R
adv
rust resistance gene. A total of 63 DNA markers previously mapped to linkage group (LG) 13 were used to screen for polymorphisms
between two parental lines HA 89 and HA-R3. A genetic map of LG 13 was constructed with 21 markers, resulting in a total map
length of 93.8 cM and an average distance of 4.5 cM between markers. Two markers, ZVG61 and ORS581, flanked the R
4
gene at 2.1 and 0.8 cM, respectively, and were located on the lower end of LG 13 within a large NBS-LRR cluster identified
previously. The PCR pattern generated by primer pair ZVG61 was unique in the HA-R3 line, compared to lines HA-R1, HA-R4, and
HA-R5, which carry other R
4
alleles. A SCAR marker linked to the rust resistance gene R
adv
mapped to LG 13 at 13.9 cM from the R
4
locus, indicating that R
adv
is not an allele of the R
4
locus. The markers tightly linked to the R
4
gene will facilitate gene pyramiding for rust resistance breeding of sunflower. 相似文献
19.
Molecular mapping of a nuclear male-sterility gene in sunflower (Helianthus annuus L.) using TRAP and SSR markers 总被引:6,自引:0,他引:6
Chen J Hu J Vick BA Jan CC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(1):122-127
A nuclear male-sterile mutant, NMS 360, induced by streptomycin from an inbred maintainer line HA 89, possesses a single recessive gene, ms9, controlling male sterility. The present study identified DNA markers linked to the ms9 gene in an F2 population derived from the cross of NMS 360 × RHA 271 and maps the ms9 gene to an existing sunflower SSR linkage map. Bulked segregant analysis was performed using the target region amplification polymorphism (TRAP) marker technique and the simple sequence repeats (SSR) technique. From 444 primer combinations, six TRAP markers linked with the ms9 gene were amplified. Two markers, Ts4p03-202 and Tt3p09-529, cosegregated with the ms9 gene. The other four markers, To3d14-310, Tt3p17-390, Ts4p23-300, and Tt3p09-531, linked with ms9 at a distance of 1.2, 3.7, 10.3, and 22.3 cM, respectively. Thirty SSR primers from 17 linkage groups of a PHA × PHB cultivated sunflower linkage map were screened among the two parents and the F2 population. SSR primer ORS 705 of linkage group 10 was tightly linked to ms9 at a distance of 1.2 cM. The ms9 gene was subsequently mapped to linkage group 10 of the public sunflower SSR linkage map. The markers that were tightly linked with the ms9 gene will be useful in marker-assisted selection of male-sterile plants among segregating populations, and will facilitate the isolation of the ms9 gene by map-based cloning. 相似文献
20.
Z. W. Zhang G. J. Ma J. Zhao S. G. Markell L. L. Qi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(1):29-39