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OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signalling in rice
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Jinjie Li Yang Li Zhigang Yin Jihong Jiang Minghui Zhang Xiao Guo Zhujia Ye Yan Zhao Haiyan Xiong Zhanying Zhang Yujie Shao Conghui Jiang Hongliang Zhang Gynheung An Nam‐Chon Paek Jauhar Ali Zichao Li 《Plant biotechnology journal》2017,15(2):183-196
Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation. 相似文献
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Genomewide identification of genes involved in the potato response to drought indicates functional evolutionary conservation with Arabidopsis plants 总被引:1,自引:0,他引:1
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Marcin Pieczynski Anna Wyrzykowska Kaja Milanowska Dominika Boguszewska‐Mankowska Barbara Zagdanska Wojciech Karlowski Artur Jarmolowski Zofia Szweykowska‐Kulinska 《Plant biotechnology journal》2018,16(2):603-614
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Genome‐wide association of drought‐related and biomass traits with HapMap SNPs in Medicago truncatula
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Yun Kang Muhammet Sakiroglu Nicholas Krom John Stanton‐Geddes Mingyi Wang Yi‐Ching Lee Nevin D. Young Michael Udvardi 《Plant, cell & environment》2015,38(10):1997-2011
Improving drought tolerance of crop plants is a major goal of plant breeders. In this study, we characterized biomass and drought‐related traits of 220 Medicago truncatula HapMap accessions. Characterized traits included shoot biomass, maximum leaf size, specific leaf weight, stomatal density, trichome density and shoot carbon‐13 isotope discrimination (δ13C) of well‐watered M. truncatula plants, and leaf performance in vitro under dehydration stress. Genome‐wide association analyses were carried out using the general linear model (GLM), the standard mixed linear model (MLM) and compressed MLM (CMLM) in TASSEL, which revealed significant overestimation of P‐values by CMLM. For each trait, candidate genes and chromosome regions containing SNP markers were found that are in significant association with the trait. For plant biomass, a 0.5 Mbp region on chromosome 2 harbouring a plasma membrane intrinsic protein, PIP2, was discovered that could potentially be targeted to increase dry matter yield. A protein disulfide isomerase‐like protein was found to be tightly associated with both shoot biomass and leaf size. A glutamate‐cysteine ligase and an aldehyde dehydrogenase family protein with Arabidopsis homologs strongly expressed in the guard cells were two of the top genes identified by stomata density genome‐wide association studies analysis. 相似文献
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Silvia Coolen Silvia Proietti Richard Hickman Nelson H. Davila Olivas Ping‐Ping Huang Marcel C. Van Verk Johan A. Van Pelt Alexander H.J. Wittenberg Martin De Vos Marcel Prins Joop J.A. Van Loon Mark G.M. Aarts Marcel Dicke Corné M.J. Pieterse Saskia C.M. Van Wees 《The Plant journal : for cell and molecular biology》2016,86(3):249-267
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The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance 总被引:3,自引:0,他引:3
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Dong‐Keun Lee Pil Joong Chung Jin Seo Jeong Geupil Jang Seung Woon Bang Harin Jung Youn Shic Kim Sun‐Hwa Ha Yang Do Choi Ju‐Kon Kim 《Plant biotechnology journal》2017,15(6):754-764
Drought has a serious impact on agriculture worldwide. A plant's ability to adapt to rhizosphere drought stress requires reprogramming of root growth and development. Although physiological studies have documented the root adaption for tolerance to the drought stress, underlying molecular mechanisms is still incomplete, which is essential for crop engineering. Here, we identified OsNAC6‐mediated root structural adaptations, including increased root number and root diameter, which enhanced drought tolerance. Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root‐specific overexpressing transgenic rice lines was less affected by drought stress than were nontransgenic controls. Genome‐wide analyses of loss‐ and gain‐of‐function mutants revealed that OsNAC6 up‐regulates the expression of direct target genes involved in membrane modification, nicotianamine (NA) biosynthesis, glutathione relocation, 3′‐phophoadenosine 5′‐phosphosulphate accumulation and glycosylation, which represent multiple drought tolerance pathways. Moreover, overexpression of NICOTIANAMINE SYNTHASE genes, direct targets of OsNAC6, promoted the accumulation of the metal chelator NA and, consequently, drought tolerance. Collectively, OsNAC6 orchestrates novel molecular drought tolerance mechanisms and has potential for the biotechnological development of high‐yielding crops under water‐limiting conditions. 相似文献
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Simultaneous expression of regulatory genes associated with specific drought‐adaptive traits improves drought adaptation in peanut
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Vemanna S. Ramu Thavarekere N. Swetha Shekarappa H. Sheela Chandrashekar K. Babitha Sreevathsa Rohini Malireddy K. Reddy Narendra Tuteja Chandrashekar P. Reddy Trichi Ganesh Prasad Makarla Udayakumar 《Plant biotechnology journal》2016,14(3):1008-1020
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The isolation of genes from the resurrection grass Sporobolus stapfianus which are induced during severe drought stress 总被引:4,自引:1,他引:3
A. D. Neale C. K. Blomstedt P. Bronson T.-N. Le K. Guthridge J. Evans D. F. Gaff & J. D. Hamill 《Plant, cell & environment》2000,23(3):265-277
A modification of the ‘cold plaque’ screening technique (Hodge et al., Plant Journal1992, 2, 257–260) was used to screen a cDNA library constructed from drought‐stressed leaf tissue of the desiccation tolerant (‘resurrection’) grass Sporobolus stapfianus. This technique allowed a large number of clones representing genes expressed at low abundance to be isolated. An examination of expression profiles revealed that several of these genes are induced in desiccation‐tolerant tissue experiencing severe drought stress. Further characterization indicated that the gene products encoded include an eIF1 protein translation initiation factor and a glycine‐ and proline‐rich protein which have not previously been associated with drought stress. In addition, genes encoding a serine/threonine phosphatase type 2C, a tonoplast‐intrinsic protein (TIP) and an early light‐inducible protein (ELIP) were isolated. A number of these genes are expressed differentially in desiccation‐tolerant and desiccation‐sensitive tissues, suggesting that they may be associated with the desiccation tolerance response of S. stapfianus. The results indicate that there may be unique gene regulation processes occurring during induction of desiccation tolerance in resurrection plants which allow different drought‐responsive genes to be selectively expressed at successive levels of water loss. 相似文献
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Nuria de María María ngeles Guevara Pedro Perdiguero María Dolores Vlez Jos Antonio Cabezas Miriam Lpez‐Hinojosa Zhen Li Luís Manuel Díaz Alberto Pizarro Jos Antonio Mancha Lieven Sterck David Snchez‐Gmez Clia Miguel Carmen Collada María Carmen Díaz‐Sala María Teresa Cervera 《Ecology and evolution》2020,10(18):9788-9807
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Xiao‐Juan Xing Ri‐He Peng Bo Zhu Jian‐Jie Gao Quan‐Hong Yao 《Physiologia plantarum》2016,156(2):164-175
The plant‐specific tau class of glutathione S‐transferases (GSTs) is often highly stress‐inducible and expressed in a tissue‐specific manner, thereby suggesting its important protective roles. Although activities associated with the binding and transport of reactive metabolites have been proposed, little is known about the regulatory functions of GSTs. Expression of AtGSTU19 is induced by several stimuli, but the function of this GST remains unknown. In this study, we demonstrated that transgenic over‐expressing (OE) plants showed enhanced tolerance to different abiotic stresses and increased percentage of seed germination and cotyledon emergence. Transgenic plants exhibited an increased level of proline and activities of antioxidant enzymes, along with decreased malonyldialdehyde level under stress conditions. Real‐time polymerase chain reaction (PCR) analyses revealed that the expression levels of several stress‐regulated genes were altered in AtGSTU19 OE plants. These results indicate that AtGSTU19 plays an important role in tolerance to salt/drought/methyl viologen stress in Arabidopsis. 相似文献