共查询到20条相似文献,搜索用时 62 毫秒
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Vahid Omidvar Siti Nor Akmar Abdullah Amir Izadfard Chai Ling Ho Maziah Mahmood 《Planta》2010,232(4):925-936
The 1,053-bp promoter of the oil palm metallothionein gene (so-called MSP1) and its 5′ deletions were fused to the GUS reporter
gene, and analysed in transiently transformed oil palm tissues. The full length promoter showed sevenfold higher activity
in the mesocarp than in leaves and 1.5-fold more activity than the CaMV35S promoter in the mesocarp. The 1,053-bp region containing
the 5′ untranslated region (UTR) gave the highest activity in the mesocarp, while the 148-bp region was required for minimal
promoter activity. Two positive regulatory regions were identified at nucleotides (nt) −953 to −619 and −420 to −256 regions.
Fine-tune deletion of the −619 to −420 nt region led to the identification of a 21-bp negative regulatory sequence in the
−598 to −577 nt region, which is involved in mesocarp-specific expression. Gel mobility shift assay revealed a strong interaction
of the leaf nuclear extract with the 21-bp region. An AGTTAGG core-sequence within this region was identified as a novel negative
regulatory element controlling fruit-specificity of the MSP1 promoter. Abscisic acid (ABA) and copper (Cu2+) induced the activity of the promoter and its 5′ deletions more effectively than methyl jasmonate (MeJa) and ethylene. In
the mesocarp, the full length promoter showed stronger inducibility in response to ABA and Cu2+ than its 5′ deletions, while in leaves, the −420 nt fragment was the most inducible by ABA and Cu2+. These results suggest that the MSP1 promoter and its regulatory regions are potentially useful for engineering fruit-specific
and inducible gene expression in oil palm. 相似文献
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Gon-Sup Kim Yeoung-Gyu Ko Oh-Sung Park Hyoung Joon Park Phil-Ok Koh Kyu-Woan Cho Kwan-Sik Min Hwan-Hoo Seong Chung-Kil Won Jae-Hyeon Cho 《Biotechnology letters》2009,31(8):1173-1181
We identified a 3.4-kb 5′-flanking region of the rPL-I gene and examined its promoter activity using rat trophoblast Rcho-1
cells. A regulatory element between base pairs (bp) −2,487 and −2,310 in the 5′-flanking region was essential for maximum
promoter activity of the rPL-I gene. This regulatory element was further characterized between bp −2,443 to −2,415 and −2,374
to −2,345. Electrophoretic mobility shift analysis showed that the interaction of nuclear extract proteins from differentiated
Rcho-1 cells was inhibited by competition with a GATA-like sequence in the promoter, but not by a mutated GATA sequence. Moreover,
the promoter activity of 2487 eLuc containing two novel GATA sites was significantly elevated by co-transfection of a GATA-2
expression vector in proliferating Rcho-1 cells. Our results demonstrate that GATA-2 is involved in multiple promoter regions
to activate the specific expression of the rPL-I gene in placental tissue.
Gon-Sup Kim and Yeoung-Gyu Ko are contributed equally to this work. 相似文献
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We isolated and characterized a pollen-preferential gene, BAN102, from Chinese cabbage and analyzed the activity of its promoter. There were three or four copies of the BAN102 gene in the Chinese cabbage genome that specifically expressed in pollen and pollen tube. There were 2137 bp of BAN102 genomic clone comprising 186 bp of protein coding region, and 1178 bp of 5′ and 773 bp of 3′ non-coding regions. TATA box
were located at 1071 nt of the promoter region while the polyadenylation signal and polyadenylation site were at 1470 and
1486 nt of the 3′ non-coding region. BLAST search of BAN102 sequence showed that coding region of BAN102 gene was the greatest percent similarity with arabinogalactan protein (AGP23) gene from Arabidopsis thaliana. Promoter analysis using GUS gene as a reporter showed that the pollen-specificity of BAN102 resided within the −112 to −44 bp of proximal promoter from the transient expression in tobacco and Chinese cabbage plants. 相似文献
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