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1.
Huang Guanrong Xiong Shiqin Zhao Qiuhui Wang Yinyin Reng Fangli Ye Xiongjun Chang Zhijie 《Frontiers of Biology in China》2006,1(2):104-109
Sef is a transmembrane protein inhibiting FGF signaling. To determine the correlation of Sef with human diseases, Sef expression
patterns were observed in cell lines and human cancer tissues. Western blot using anti-hSef antibodies showed that hSef, when
expressed in Cos7 cells gave a molecular mass of 100 KD as compared with 80 KD in an in vitro translation assay suggesting occurrence of glycosylation at the potential N-linked glycosylation sites in the extracellular
domain. Northern blot showed that hSef was mainly expressed in human kidney and testis. RT-PCR analysis showed a widely spread
expression pattern in several cell lines. Immunohistochemical analysis revealed a high expression level of hSef in kidney,
testis, and the corresponding carcinoma tissues. Results demonstrated that Sef might be up-regulated in the cancer tissues
suggesting a possible role of Sef in pathophysiology of human diseases.
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Translated from Chinese Journal of Biochemistry and Molecular Biology, 2005, 21 (2) [译自: 中国生物化学与分子生物学报, 2005,21(2)] 相似文献
2.
Guanrong Huang Shiqin Xiong Qiuhui Zhao Yinyin Wang Fangli Reng Xiongjun Ye Zhijie Chang 《生物学前沿》2006,1(2):104-109
Sef is a transmembrane protein inhibiting FGF signaling.To determine the correlation of Sef with human diseases,Sef expression patterns were observed in cell lines and human cancer tissues.Western blot using anti-hSef antibodies showed that hSef,when expressed in Cos7 cells gave a molecular mass of 100 KD as compared with 80 KD in an in vitro translation assay suggesting occurrence of glycosylation at the potential N-linked glycosylation sites in the extracellular domain.Northern blot showed that hSef was mainly expressed in human kidney and testis.RT-PCR analysis showed a widely spread expression pattern in several cell lines.Immunohistochemical analysis revealed ahigh expression level of hSef in kidney,testis,and the corresponding carcinoma tissues.Results demonstrated that Sef might be up-regulated in the cancer tissues suggesting a possible role of Sef in pathophysiology of human diseases. 相似文献
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Yoichi Shinkai Michihiro C. Yoshida Keiko Maeda Tetsuji Kobata Kazuo Maruyama Junji Yodoi Hideo Yagita Ko Okumura 《Immunogenetics》1989,30(6):452-457
Human perforin cDNA was isolated and the complete nucleotide sequence of the gene determined. The deduced amino acid sequence
of human perforin showed 68.4% similarity to that of mouse perforin. RNA blot analysis of the human perforin gene revealed
that the gene product is expressed preferentially in killer-type cells among cell lines tested, and in large granular lymphocytes
among the peripheral blood mononuclear cells. In situ hybridization analysis with a human perforin cDNA probe revealed that
the human perforin (PFP) gene is located on chromosome17q11-21.
The nucleotide sequence data reported in this paper have been submitted to the GeBank nucleotide sequence database and have
been assigned the accession number M28393. 相似文献
5.
Shuijie Li Fredéric Lamarche Romain Charton Christian Delphin Olivier Gires Arnaud Hubstenberger Uwe Schlattner Denis Rousseau 《Gene》2014
ATAD3 (ATPase family AAA-Domain containing protein 3) is a mitochondrial inner membrane ATPase with unknown but vital functions. Initial researches have focused essentially on the major p66-ATAD3 isoform, but other proteins and mRNAs are described in the data banks. Using a set of anti-peptide antibodies and by the use of rodent and human cell lines and organs, we tried to detail ATAD3 gene expression profiles and to verify the existence of the various ATAD3 isoforms. In rodent, the single ATAD3 gene is expressed as a major isoform of 67 kDa, (ATAD3l; long), in all cells and organs studied. A second isoform, p57-ATAD3s (small), is expressed specifically throughout brain development and in adult, and overexpressed around the peri-natal period. p57-ATAD3s is also expressed in neuronal and glial rodent cell lines, and during in vitro differentiation of primary cultured rat oligodendrocytes. Other smaller isoforms were also detected in a tissue-specific manner. In human and primates, ATAD3 paralogues are encoded by three genes (ATAD3A, 3B and 3C), each of them presenting several putative variants. Analyzing the expression of ATAD3A and ATAD3B with four specific anti-peptide antibodies, and comparing their expressions with in vitro expressed ATAD3 cDNAs, we were able to observe and define five isoforms. In particular, the previously described p72-ATAD3B is confirmed to be in certain cases a phosphorylated form of ATAD3As. Moreover, we observed that the ATAD3As phosphorylation level is regulated by insulin and serum. Finally, exploring ATAD3 mRNA expression, we confirmed the existence of an alternative splicing in rodent and of several mRNA isoforms in human. 相似文献
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Kawano T Horiguchi-Yamada J Iwase S Furukawa Y Kano Y Yamada H 《Molecular and cellular biochemistry》2004,258(1-2):25-33
K562 cells contain a Bcr-Abl chimeric gene and differentiate into various lineages in response to different inducers. We studied the role of the mitogen-activated protein kinase (MAPK) kinase 1 (MEK1)/extracellular signal-regulated kinase (ERK) pathway during the erythroid differentiation of K562 cells induced by tyrosine kinase inhibitors (herbimycin A or STI571), using genetically modified cells (constitutively MEK1-activated K562: K562/MEK1, and inducible ERK-inactivated K562: K562/CL100). Basal expression of glycophorin A was markedly reduced in K562/MEK1 cells compared with that in parental cells, while it was augmented in K562/CL100 cells. Herbimycin A and STI571 differentiated K562 cells accompanying with the transient down-regulated ERK. Moreover, the erythroid differentiation was markedly suppressed in K562/MEK1 cells, and early down-regulation of ERK activity was not observed in these cells. In contrast, the induction of ERK-specific phosphatase in K562/CL100 cells potentiated erythroid differentiation. Once the phosphatase was induced, the initial ERK activity became repressed and its early down-regulation by the inhibition of Bcr-Abl was marked and prolonged. These results demonstrate that the erythroid differentiation of K562 cells induced by herbimycin A or STI571 requires the down-regulation of MEK1/ ERK pathway. 相似文献
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Wang R An J Ji F Jiao H Sun H Zhou D 《Biochemical and biophysical research communications》2008,373(1):151-154
Low expression of the oxidative stress sensor Keap1 is thought to be involved in carcinogenesis. However, the mechanisms responsible for inactivation of the Keap1 gene remain unknown. We investigated Keap1 expression using RT-PCR and found that it was downregulated in lung cancer cell lines and tissues when compared with a normal bronchial epithelial cell line. Treatment with 5-Aza-2′-deoxycytidine restored Keap1 expression in lung cancer cell lines, indicating the silencing mechanism to be promoter methylation. Moreover, we evaluated cytosine methylation in the Keap1 promoter and demonstrated that the P1 region, including 12 CpG sites, was highly methylated in lung cancer cells and tissues, but not in normal cells. Importantly, we found evidence that three specific CpG sites (the 3rd, 6th, and 10th CpGs of P1) might be binding sites for proteins that regulate Keap1 expression. Thus, our results suggest for the first time that Keap1 expression is regulated by an epigenetic mechanism in lung cancer. 相似文献
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M W Berchtold P Epstein A L Beaudet M E Payne C W Heizmann A R Means 《The Journal of biological chemistry》1987,262(18):8696-8701
10.
Expression and localization of the two small proteoglycans biglycan and decorin in developing human skeletal and non-skeletal tissues 总被引:20,自引:0,他引:20
P Bianco L W Fisher M F Young J D Termine P G Robey 《The journal of histochemistry and cytochemistry》1990,38(11):1549-1563
The messenger RNAs and core proteins of the two small chondroitin/dermatan sulfate proteoglycans, biglycan and decorin, were localized in developing human bone and other tissues by both 35S-labeled RNA probes and antibodies directed against synthetic peptides corresponding to nonhomologous regions of the two core proteins. Biglycan and decorin expression and localization were substantially divergent and sometimes mutually exclusive. In developing bones, spatially restricted patterns of gene expression and/or matrix localization of the two proteoglycans were identified in articular regions, epiphyseal cartilage, vascular canals, subperichondral regions, and periosteum, and indicated the association of each molecule with specific developmental events at specific sites. Study of non-skeletal tissues revealed that decorin was associated with all major type I (and type II) collagen-rich connective tissues. Conversely, biglycan was expressed and localized in a range of specialized cell types, including connective tissue (skeletal myofibers, endothelial cells) and epithelial cells (differentiating keratinocytes, renal tubular epithelia). Biglycan core protein was localized at the cell surface of certain cell types (e.g., keratinocytes). Whereas the distribution of decorin was consistent with matrix-centered functions, possibly related to regulation of growth of collagen fibers, the distribution of biglycan pointed to other function(s), perhaps related to cell regulation. 相似文献
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Norma Morrison Stephen B. Harrap Jeffrey L. Arriza Elizabeth Boyd J. Michael Connor 《Human genetics》1990,85(1):130-132
Summary The gene for human mineralocorticoid receptor (hMR), previously mapped to chromosome 4, has been further localized to 4q31.1 by in situ hybridization using a biotinylated 3.75kb human cDNA clone encoding the primary amino acid sequence of hMR as a probe. Preliminary comparative mapping studies in orangutan (Pongo pygmaeus) suggest localization of the probe to the long arm of chromosome 3. 相似文献
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Norma Morrison Craig Simpson Linda Fothergill-Gilmore Elizabeth Boyd J. Michael Connor 《Human genetics》1992,89(1):105-106
Summary A cDNA for human platelet 6-phosphofructokinase (PFKP) has been isolated from a human Raji cell line cDNA library. Using this cDNA as a probe, the gene for human PFKP, previously mapped to chromosome 10pter-p11.1, has been further localized to 10p15 by non-isotopic in situ hybridization. 相似文献
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Summary The gene for the human glucocorticoid receptor, previously mapped to chromosome 5, has been further localised to 5q31 by in situ hybridisation using a biotinylated 4.3-kb cDNA probe. 相似文献
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Expression of receptors for melanin-concentrating hormone (MCH) in different tissues and cell lines 总被引:2,自引:0,他引:2
Schlumberger SE Talke-Messerer C Zumsteg U Eberle AN 《Journal of receptor and signal transduction research》2002,22(1-4):509-531
Melanin-concentrating hormone (MCH) is a potent orexigenic neuropeptide and a physiological antagonist of alpha-melanocyte-stimulating hormone (alpha-MSH) in the brain as well as at peripheral sites, including the pigmentary systems of specific vertebrates. Two receptor subtypes for MCH, MCH-R1 and MCH-R2, have been cloned, but other receptor subtypes are likely to exist. Based on our own data and the current literature, we have compared the expression of different receptors for MCH in various mammalian cell lines and tissues. Summarizing all data currently available, we conclude that the two cloned MCH receptors, MCH-R1 and MCH-R2, exhibit differences in their expression pattern, although MCH-R1 is generally colocalized in all tissues where MCH-R2 expression is found. It appears that MCH-R1 is more abundant and has a wider distribution pattern than MCH-R2. Other hypothetical MCH-R subtypes may be expressed in specific tissues, e.g., in the pigment cell system. 相似文献
20.
Structural organization and chromosomal assignment of the gene encoding endothelin 总被引:18,自引:0,他引:18
K D Bloch S P Friedrich M E Lee R L Eddy T B Shows T Quertermous 《The Journal of biological chemistry》1989,264(18):10851-10857
Endothelin is a 21-amino acid vasoconstrictor synthesized and secreted by vascular endothelial cells. The human peptide is derived from a 212-amino acid precursor, preproendothelin. A nearly full length clone containing DNA complementary to human preproendothelin mRNA was isolated, and its nucleotide sequence was determined. Using this cDNA as a probe, the genomic organization of the human endothelin gene was determined and the promoter region delineated. The gene contains five exons and four intervening sequences. Nucleotide sequences encoding endothelin are contained within the second exon, and the third exon specifies a portion of preproendothelin that is homologous to endothelin. The second and third exons may represent descendants of a common progenitor exon. The 3'-untranslated portion of the gene contains a 250-base pair region that is highly conserved between human and porcine genomes and may have an important role in endothelin mRNA stability. On the basis of DNA isolated from human-mouse somatic hybrid cell lines, the endothelin gene was assigned to human chromosome 6. 相似文献