Diterpenes from Croton salutaris

The twigs of Croton salutaris afforded three new acyclic diterpenes and a new tricyclic diterpene as well as a known compound, sonderianol. The structures of three acyclic diterpenes [(10E)-3,12-dihydroxy-3,7,11,15-tetramethyl-1,10,14-hexadecatrien-5,13-dione, (6E,10E)-3,12-dihydroxy-3,7,11,15-tetramethyl-1,6,10,14-hexadecatetraen-5,13-dione and (6Z,10E)-3,12-dihydroxy-3,7,11,15-tetramethyl-1,6,10,14-hexadecatetraen-5,13-dione] and a tricyclic diterpene [12-hydroxy-13-methylpodocarpa-9,11,13-trien-3-one] were determined by spectral methods.     

Rabdosinate调节生长素极性运输蛋白PIN1、PIN3和PIN4抑制拟南芥幼苗根生长

利用3种拟南芥生长素极性运输外运载体突变体及4种转基因株系研究了二萜rabdosinate抑制拟南芥幼苗主根及侧根生长的作用机制。结果显示,60~80 μmol·L^-1的rabdosinate显著抑制野生型拟南芥幼苗主根生长及侧根形成,而对突变体pin1、pin2和pin3主根未显示明显的抑制效应,对侧根的抑制减弱;发现rabdosinate （60~80 μmol·L^-1）引起生长素报告株系根尖DR5活性升高,并增加融合蛋白PIN1-GFP丰度以及减少PIN3-GFP和PIN4-GFP的丰度。推断rabdosinate可通过增加PIN1丰度促进了根部生长素向顶运输,而减少PIN3丰度降低根尖部生长素的横向转运,引起了生长素在根尖部的累积及生长素浓度梯度的改变,进而抑制幼苗主根生长及侧根发育。     

A hardwickiic acid derivative from Pulicaria gnaphalodes

The aerial parts of Pulicaria gnaphalodes afrorded a new clerodane lactone closely related to hardwickiic acid.     

鹅膏环肽毒素生源合成的研究进展

在蘑菇目Agaricales中,已知鹅膏属Amanita、环柄菇属Lepiota和盔孢伞属Galerina 3个属的部分物种能产生剧毒的鹅膏环肽毒素。全球90%以上的致死性蘑菇中毒事件是由含鹅膏环肽蘑菇导致。上述3个属的剧毒蘑菇虽然亲缘关系较远,但却可以合成同一种鹅膏环肽毒素,且使用了大致相同的生源合成代谢途径,涉及多个毒素合成基因,采用了特殊的组合式合成机制。本文总结了鹅膏环肽毒素合成途径研究的最新进展,指出了当前工作中遇到的一些难题,对未来研究的趋势进行了展望。     

基于转录组测序分析甜菜夜蛾卵巢细胞离体培养成系进程

【目的】本研究旨在分析甜菜夜蛾Spodoptera exigua蛹卵巢细胞建立细胞系的整个过程,探究细胞由体内到体外培养过程中其基因表达在转录水平的变化,为昆虫体外培养模型的建立提供理论基础。【方法】利用Illumina Hiseq测序平台对甜菜夜蛾蛹卵巢细胞离体培养过程中各阶段的细胞分别进行转录组测序,对获得注释的差异表达基因及其相关信号通路进行分析;通过荧光定量PCR对部分细胞周期相关基因(cycd和cdk4)、调控基因(cdc20,apc1,skp2和mad1)、增殖相关分子标志物(mcm4和pcna)在甜菜夜蛾卵巢细胞离体培养过程中的转录进行验证。【结果】甜菜夜蛾蛹卵巢细胞离体培养过程包括5个阶段:解剖获得离体的卵巢组织,卵巢组织贴壁培养后游离出原代细胞,细胞转化重新具备增殖能力,成功首次传代,以及能够连续传代15代以上建立细胞系。上述5个阶段的细胞经转录组测序、数据组装后共获得46 796条unigenes序列,组装得到序列长度完整性好;转录本unigenes序列拼接长度分布合理,样本碱基Q30均在94%以上。通过KEGG数据库获得注释的unigenes有1 473条,参与细胞过程紧密相关的20条信号通路,其中有92条unigenes在细胞周期信号通路中获得注释。聚类分析表明,在体内处于快速发育状态的卵巢细胞与同样处于增殖状态的细胞系基因表达模式非常接近。原代细胞由短暂停滞生长至成簇细胞的转化关键期,筛选到差异表达基因619个,cdk4在离体培养期表达量显著降低,cycd在细胞转化关键期之后表达量显著升高,cdc20,apc1,skp2和mad1在卵巢组织和细胞系的表达量显著高于原代细胞、转化关键期细胞和首次传代细胞的。从原代细胞至传代后,cycd的表达显著升高8.7倍,显著高于mcm4和pcna的变化水平。【结论】甜菜夜蛾卵巢细胞离体培养过程中5个阶段的细胞转录组测序获得的序列质量符合数据分析的基本要求。筛选获得了甜菜夜蛾蛹卵巢细胞经离体培养过程中的差异表达基因。原代细胞逆转增殖可能与cdk4,cycd,skp2和mad1等细胞周期调控基因表达有关。另外,cycd可作为原代细胞具备传代能力的标志物。     

不同传播方式的内生真菌感染对羽茅的生理生态影响

通过田间试验, 研究了垂直传播的Neotyphodium属内生真菌和水平传播的Epichloë属内生真菌对羽茅(Achnatherum sibiricum)生理生态的影响。结果表明: Neotyphodium属内生真菌感染对羽茅的株高和叶长有显著的正效应, 而Epichloë属内生真菌感染对羽茅株高和叶长的增益作用较少。感染Neotyphodium属内生真菌的羽茅最大净光合速率显著高于感染Epichloë属内生真菌的羽茅, Neotyphodium属内生真菌显著提升了羽茅的气孔限制值和水分利用效率, 而实验测定的第一年结果显示: Epichloë属内生真菌对羽茅的各个光合指标具有明显的负效应(胞间CO₂浓度除外)。Neotyphodium属内生真菌感染使得宿主植物积累的可溶性糖含量显著高于感染Epichloë属内生真菌的羽茅和不染菌的羽茅植株。感染Epichloë属内生真菌的羽茅中氮含量显著高于感染Neotyphodium属内生真菌的羽茅。总之, 内生真菌的传播方式是影响羽茅生理生态学特性的一个因素, 并且垂直传播的内生真菌对羽茅生长和生理特性的益处高于水平传播的内生真菌。     

家蚕幼虫BmToll9基因对肽聚糖和金黄色葡萄球菌的响应表达

【目的】Toll信号通路是昆虫中重要的免疫信号通路,其中Toll受体在保持Toll通路的正常免疫应答、抵抗外源病原体中起到关键的作用。本研究旨在探究肽聚糖和金黄色葡萄球菌Staphylococcus aureus对家蚕Bombyx mori Toll受体基因BmToll9-1和BmToll9-2表达的影响。【方法】将革兰氏阳性菌细胞壁主要成分肽聚糖和革兰氏阳性菌金黄色葡萄球菌分别注射感染家蚕5龄第1天幼虫,诱导其发生免疫反应;采用实时荧光定量PCR分析注射后不同感染时间点BmToll9-2和BmToll9-1基因在家蚕幼虫中肠、表皮、脂肪体和丝腺中的相对表达水平。【结果】往家蚕5龄幼虫中注射肽聚糖或金黄色葡萄球菌后,BmToll9-2基因出现了时间和组织的差异性表达。注射肽聚糖和金黄色葡萄球菌均能诱导5龄幼虫中肠BmToll9-2基因的表达上调,注射肽聚糖和金黄色葡萄球菌分别在3和6 h时对基因表达的诱导效果最好,且注射金黄色葡萄球菌比注射肽聚糖对基因表达的诱导效果更好。注射金黄色葡萄球菌能引起5龄幼虫表皮、脂肪体和丝腺中BmToll9-2基因的表达上调,分别于注射后24, 6和24 h时诱导效果最好。注射金黄色葡萄球菌亦能诱导同源的BmToll9-1基因的上调表达。【结论】家蚕幼虫BmToll9基因在肽聚糖或金黄色葡萄球菌注射处理后均能在不同组织中发生上调表达,推测BmToll9基因参与了家蚕对肽聚糖和金黄色葡萄球菌的免疫反应。     

高原鼠兔干扰对高寒草甸β多样性的影响

β多样性反映生物群落沿某一环境梯度的物种周转速率, 该研究尝试采用β多样性揭示植物群落随小型啮齿草食动物干扰梯度变化的生态过程。该研究利用野外随机样地的采集数据, 分析了高原鼠兔(Ochotona curzoniae)不同干扰强度下Whittaker指数的变化特征, 并利用群落二元丰富度的方差分解法, 确定了单个物种(SCBD)和单个干扰位点(LCBD)对β多样性的贡献。主要结果: 随高原鼠兔干扰强度增加, 植物群落内物种周转速率呈先增加后降低的趋势; 占据位点数居中的物种对区域内的β多样性贡献较大, 其中冰草(Agropyron cristatum)、臭蒿(Artemisia hedinii)、小花草玉梅(Anemone rivularis var. flore-minore)等单个物种对整个区域内β多样性的贡献最为突出; 整个区域内干扰位点T₀ (高原鼠兔干扰强度为0)对区域β多样性贡献值最大, LCBD值和该位点的群落丰富度呈显著负相关关系, 但与高原鼠兔干扰强度无显著关联。说明重点保护LCBD值高的干扰位点所在的高寒草甸, 以及SCBD值较高的冰草、臭蒿、小花草玉梅, 对保护高原鼠兔存在时高寒草甸植物群落多样性具有重要意义。     

农杆菌介导Txpam基因转化烟曲霉TMS-26及其产紫杉醇效果评价

为研究红豆杉紫杉醇合成途径限速酶基因功能及其对内生真菌烟曲霉TMS-26发酵产紫杉醇的影响,以曼地亚红豆杉愈伤组织制备cDNA作为模板扩增苯丙氨酸氨基变位酶基因（Txpam）,构建重组质粒pGEX-4T-1-Txpam,转入大肠杆菌中进行异源诱导表达,经亲和层析纯化,获取重组酶TxPAM并验证其酶活性。构建pCAMBIA1302-Txpam质粒,转化农杆菌感受态细胞,利用农杆菌介导的转化体系获得转化子并优化转化条件,结合插入片段携带的分子标记和目的基因进行转化子验证,同时培养转化菌株并检测紫杉醇产量。结果表明：纯化获取的重组酶TxPAM,经HPLC检测具有将α-苯丙氨酸催化为β-苯丙氨酸的功能;在最优转化条件下,转化子数目达到471个/10⁶个孢子;根据基因hyg和Txpam的克隆以及测序结果,说明成功构建了基因工程菌株,通过对其发酵条件进行优化,紫杉醇产量达到721.87μg/L。     

Allelic variants interaction of CLOCK gene and G-protein beta3 subunit gene with diurnal preference

The 3111 C/T single nucleotide polymorphism (SNP) in the CLOCK gene and the 825C/T SNP in the G-protein β3 subunit gene (GNB3) have been reported to influence diurnal preference. This study has attempted to characterize the association between the CLOCK gene and GNB3 polymorphisms and diurnal preference in healthy Korean college students. All subjects completed the 13-item Composite Scale for Morningness (CSM). The interaction between the 3111 C/T SNP in the CLOCK gene and the 825 C/T SNP in the GNB3 gene significantly influenced diurnal preference, according to the CSM Performance subscore (F=10.94, p=0.001). However, when the different polymorphisms of the two genes were analyzed independently, no direct correlations with diurnal preference were detected. The CLOCK gene 3111 C/T SNP and GNB3 gene 825 C/T SNP were found to manifest a gene-gene interaction that affects diurnal preference.     

松树蜂的产卵行为

【目的】研究松树蜂Sirex noctilio的产卵行为,明确其产卵能力,为评估其繁殖潜力和危害能力提供基础数据。【方法】于黑龙江省大庆市杜尔伯特蒙古族自治县新店林场采集被松树蜂危害的寄主樟子松Pinus sylvestris var.mongolica木段上获得虫源。在室内条件下观察和分析松树蜂产卵的行为过程及规律。解剖松树蜂在樟子松木段上的产卵孔,并观察其结构特征。【结果】松树蜂羽化时即性成熟,能够马上进行交配和产卵。松树蜂雌成虫一次完整的产卵过程主要分为4个动作:树皮钻孔、木质部钻刺、产卵(注入有毒黏液和共生真菌Amylosereum areolatum)和产卵器拔出。产卵时间在360～540 s之间的产卵频数最多,占产卵总频数的41.40%;产卵过程中木质部钻刺用时最长,至少占整个产卵过程用时的90%。松树蜂雌成虫在一个产卵孔处会进行1～4次产卵,产卵时间和产卵次数呈显著正相关,当产卵时间t<360 s时,进行了1次产卵;当360 s≤t<540 s时,进行了2次产卵;当540 s≤t<780 s时,进行了3次产卵;当t≥780 s时,进行了4次或更多次产卵。松树蜂在一个产卵孔处进行1, 2, 3和4次产卵的比例分别为21.66%, 41.40%, 27.39%和9.55%。【结论】松树蜂雌成虫在一个产卵孔处的产卵时间和产卵次数呈正相关。利用产卵时间和产卵次数的关系,在只调查产卵时间的情况下,可以推断产卵次数。松树蜂在一个产卵孔处产卵的次数多,对寄主樟子松危害大。     

Cryptocin和5ʹ-epi-cryptocin的立体构型及抗菌活性

在活性追踪的指导下,从一株落羽杉内生真菌Pezicula neosporulosa SC1337玉米发酵物中分离得到两个tetramic acid类抗菌活性化合物5ʹ-epi-cryptocin (1)和cryptocin (2),通过核磁共振(NMR)谱、质谱分析和X-射线单晶衍射确定了它们的绝对构型。本文首次从同一真菌代谢产物中分离得到这对差向异构体,并报道了化合物1的晶体结构和2的NMR数据。活性测试表明化合物1和2对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌有中等强度的抑制活性,MIC均为30 μg/mL;载样量为200 μg时两者对灰巨座壳菌、稻绿核菌和新月弯孢霉的抑菌圈半径均为7.5、5.0和2.0 mm,对意大利青霉和指状青霉均未显示抑菌圈,说明这对差向异构体的5ʹ-位构型差异对其抗菌活性无明显影响。     

α-Chymotrypsin shows higher activity in water as well as organic solvents after three phase partitioning

Alpha-Chymotrypsin was found to show a 119% increase in activity after three phase partitioning. The k_cat/K_m of the partitioned enzyme (TPP-C) for hydrolysis of Bz-Tyr-OEt in aqueous medium at 25°C was found to be 48.3×10⁴ mM^-1 min^-1 as compared to the corresponding value of 17.7×10⁴ mM^-1 min^-1 for the untreated control (C). The λ_max of the fluorescence emission spectrum of TPP-C showed 178% increase in the quantum yield when compared to C. TPP-C showed a 2.94 and 3.58 fold increase (as compared to C) in initial rates for formation of the ester Ac-Phe-OEt (from Ac-Phe and ethanol) in low water containing toluene and n-octane, respectively. It was found that TPP-C also showed the phenomenon of pH memory. At 5% (v v^-1) water (in t-amyl alcohol), while no esterification was observed with C, TPP-C still showed significant level of esterification activity.     

Surviving the end-Ordovician extinctions: evidence from the earliest Silurian brachiopods of northeastern Jiangxi and western Zhejiang provinces, East China

Earliest Rhuddanian (Silurian) brachiopods are recorded from the basal part of the Lower Llandovery Shiyang and Anji formations in western Zhejiang and northeastern Jiangxi provinces, East China. Associated graptolites including Normalograptus jerini indicate the lowest Rhuddanian Akidograptus ascensus Biozone. The surviving brachiopod fauna includes 19 genera dominated by orthids and strophomenids, whereas pentamerids and atrypids that inhabited mainly warmer water regimes, and were almost absent in the cool/cold Hirnantia Fauna, occur rarely in the studied fauna. Each family is represented by a single genus that seeded their recovery. The predominance of these long-ranging and widely distributed genera is one of major characters of the brachiopod survival in east China. From qualitative and quantitative analysis of faunal composition, diversity and abundance, with evidences from palaeoecology and palaeogeography, the Levenea qianbeiensis Association, Katastrophomena-Leptaena-Levenea Association, and Glyptorthis-Epitomyonia-Levenea Association are recognized and assigned to BA (Benthic Assemblage) 2, BA3, and an ecozone close to the BA3-4 boundary respectively. No Lazarus genera are recorded in this study. Skenidioides and Epitomyonia were chiefly regarded as deeper-water taxa in the Ordovician and Silurian, but are recorded from shallow-water in east China during the early Rhuddanian, indicating an ecologic experiment with these taxa migrating from deep into shallower, better-oxygenated sites at the crisis time and during the subsequent survival interval. This study further demonstrates that the brachiopod faunal turnover after the end-Ordovician extinctions may not have been completed until the late Rhuddanian in South China.     

Chemotaxonomy of Gentianopsis: Xanthones, C-glycosylflavonoids and carbohydrates

In the genus Gentiana s.l. (Gentianaceae), the status of the section Crossopetalum Froel, is still very much debated. On Morphological grounds. Ma regarded this section as an independent genus which he called Gentianopsis. In this paper we discuss this proposal, taking into account chemotaxonomic data (xanthones, carbohydrates and C-glycosylflavones) arising from an examination of leaves and flowers of 13 species including the type-species Gentianopsis barbata. Isoscoparin, primeverose. 1,3,7- and 1,3,7,8-O-substituted xanthones were present in all species, while 1,3,5,8-O-substituted xanthones were absent. These criteria clearly differentiate the group from Gentianella (occurrence of 1,3,5,8-O-substituted xanthones, absence of 1,3,7-O-substituted xanthones, presence of swertisin, absence of primeverosel in which they were previously included. Furthermore, a comparison of Crossopetalum with two species belonging to Gentiana s.s. (the type G. lutea L. and G. orbicularis Schur.) discloses some analogy with respect to xanthone composition (only 1,3,7- or 1,3,7,8-substitution, respectively). However, the presence of gentianoise in Gentiana s.s., in contrast to its absence in Gentianopsis, indicates that the latter should be excluded from Gentiana. The assessment on morphological grounds of the validity of the genus Gentianopsis Ma is thus reinforced. The evolutionary signification of the chemical characters and the phyletic relationships of Gentianopsis with the other genera of the Gentianinae are discussed.     

毕赤酵母底盘芳香族氨基酸合成途径改造生产肉桂酸及对香豆酸*

目的:改造毕赤酵母使其异源合成类黄酮生物合成途径的重要中间体肉桂酸、对香豆酸,并优化前体芳香族氨基酸生物合成途径以提高毕赤酵母的生产能力。方法:在毕赤酵母GS115中利用乙醇诱导型人工转录系统表达Rhodotorula glutinis来源的苯丙氨酸解氨酶,并在该重组菌株中分别过表达胞内芳香族氨基酸生物合成途径中的关键酶或其突变体以进行优化。结果:异源表达苯丙氨酸解氨酶可使毕赤酵母将自身产生的L-苯丙氨酸、L-酪氨酸转化为肉桂酸(38.8 mg/L)、对香豆酸(34.2 mg/L),而通过过表达相关酶进行优化,最终肉桂酸和对香豆酸的产量分别达到124.1 mg/L和302.0 mg/L。结论:利用新的异源宿主毕赤酵母成功合成了肉桂酸、对香豆酸,并对胞内的芳香族氨基酸生物合成途径进行了优化,表明毕赤酵母具有生产黄酮类化合物的应用潜力,也为其他芳香族氨基酸衍生物或植物化合物在毕赤酵母中的异源合成奠定了基础。     

鸡PPARγ基因转录本3上游开放阅读框转录后的调控作用

过氧化物酶体增殖物激活受体γ (peroxisome proliferator-activated receptor gamma, PPARγ)是脂肪生成的关键调控因子。本实验室前期研究发现,与人和鼠等哺乳动物PPARγ基因的转录本不同,鸡PPARγ基因的多个转录本5′UTR区存在上游开放阅读框(upstream open reading frames, uORFs)。为了揭示该uORF转录后的调控作用,本研究构建了鸡PPARγ基因转录本3 (cPPARγ3)野生型5′UTR报告基因载体psiCHECK2-cPPARγ3- 5′UTR-WT和uORF突变(uATG突变为终止密码子TGA)的5′UTR报告基因载体psiCHECK2-cPPARγ3- 5′UTR-Mut。将这两个报告基因载体分别转染永生化鸡前脂肪细胞(immortalized chicken pre-adipocytes, ICPA)和鸡胚成纤维细胞DF1,检测海肾荧光素酶报告基因hRluc活性及其mRNA表达。荧光素酶报告基因检测结果显示,在ICPA细胞中,psiCHECK2-cPPARγ3-5′UTR-Mut的hRluc报告基因活性极显著高于psiCHECK2- cPPARγ3-5′UTR-WT (P<0.01);在DF1细胞中,psiCHECK2-cPPARγ3-5′UTR-Mut的hRluc报告基因活性高于psiCHECK2-cPPARγ3-5′UTR-WT,但差异不显著(P>0.05)。qRT-PCR检测hRluc基因mRNA表达结果显示,与psiCHECK2-cPPARγ3-5′UTR-WT相比,在ICPA细胞中,psiCHECK2-cPPARγ3-5′UTR-Mut转染细胞的hRluc基因的mRNA表达水平极显著降低(P<0.01);在DF1细胞中,psiCHECK2-cPPARγ3-5′UTR-Mut转染细胞后,hRluc基因的mRNA表达水平也降低,但差异不显著(P>0.05)。为进一步分析该uORF对鸡cPPARγ3的转录后调控作用,本研究又分别构建了野生型cPPARγ3真核表达载体pcDNA3.1-cPPARγ3-WT和uORF突变的cPPARγ3真核表达载体pcDNA3.1-cPPARγ3-Mut。qRT-PCR检测cPPARγ3的mRNA表达水平,结果显示,在这两种细胞中,pcDNA3.1-cPPARγ3-Mut转染细胞的cPPARγ3 mRNA表达水平均显著低于pcDNA3.1-cPPARγ3-WT转染细胞(P<0.05),但Western blot结果显示,pcDNA3.1-cPPARγ3-Mut转染细胞的PPARγ蛋白表达水平极显著高于pcDNA3.1-cPPARγ3-WT转染细胞(P<0.01)。这些研究结果表明,5′UTR区的uORF抑制鸡cPPARγ3的翻译。     

一种六妹羊肚菌的新型柄腐病害

近年来,四川等地种植的六妹羊肚菌先后爆发柄腐病害,造成严重经济损失。本研究对该病害发生情况进行了调查,从四川、河南、甘肃、贵州4个省收集的12份六妹羊肚菌柄腐病子实体,分离纯化得到37株真菌和3株细菌。根据柯赫氏法则,对所有分离纯化得到的疑似病原菌进行了回接试验,最终确定GJB-3菌株为柄腐病的病原菌。经ITS、RPB2和EF1α序列分析,结合菌落形态和显微特征鉴定柄腐病的病原菌为Fusarium nematophilum。本研究为后期羊肚菌生产病害防控提供了有效的基础数据。     

Beta2-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro

The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the β₂-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of β₂-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. β₂-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.     

转基因PnDof30拟南芥非生物胁迫下的抗性分析

Dof(DNA-binding with one finger)转录因子是植物中特有的一类转录因子,是锌指蛋白家族中的一个具有众多成员的家族,氨基酸长度一般在200~400,含有非常保守的N端和较为多变的C端。已有研究表明,Dof转录因子家族在参与植物发育的多种生理途径和调节碳氮代谢、增加氮素的吸收与利用,提高植株抗逆能力中起着重要作用。为了探究小黑杨(Populus simonii×P.nigra)中Dof30基因的抗逆能力,本研究以转基因PnDof30拟南芥为研究对象,对干旱、盐和渗透胁迫后过表达PnDof30拟南芥株系L2和野生型拟南芥WT的生理指标进行比较。发现胁迫后拟南芥株系L2的种子萌发率、根长和鲜重等指标均高于WT;同时SOD、POD、脯氨酸含量高于WT,叶绿素和MDA含量下降;胁迫后L2中的PnDof30基因表达量显著提高。这些结果表明了PnDof30基因具有抗旱、耐盐和渗透胁迫的能力,对全面了解Dof转录因子的抗逆胁迫功能具有重要的意义。