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1.
三七花蕾皂甙成分的研究   总被引:12,自引:0,他引:12  
从云南名贵药材三七(Panax notoginseng(Burk)F.H.Chen)的花蕾中分得10种化合物,经化学和光谱方法确定为:β-谷甾醇(β-Si-tosterol),胡萝卜甙(daucosterol),三七皂甙-Fe(notoginsenoside-Fe),绞股蓝皂甙-Ⅸ(gypenoside-Ⅸ),人参皂甙-Rc 和人参皂甙-Rb_3(ginsenosides-Rc和 ginsenosides-Rb_3)。其余4个微量化合物尚待进一步鉴定。  相似文献   

2.
三七中三七素的分离纯化与结构分析   总被引:1,自引:0,他引:1  
以三七(Panaxnotoginseng)为材料,经醇提、水提,得到三七素粗提物,用乙醇沉淀、正丁醇萃取去除皂苷类成分,经阳离子交换树脂柱层析,得到三七素.三七素经初步纯化含量从4.43%提高到13.98%.经过离子交换树脂柱分离后含量提高到96.46%.通过重结晶得到的无色板状晶体三七素的结构经过红外光谱,核磁共振光谱及质谱加以鉴定。  相似文献   

3.
元宝枫叶黄酮类化合物分离与鉴定   总被引:3,自引:0,他引:3  
用聚酰胺和SephadexLH20柱层析方法对元宝枫叶化学成分进行研究。分离纯化得到一种黄酮类化合物,根据理化性质及光谱数据鉴定化学结构为3,5,7,3′,4′五羟基黄酮,即槲皮素。这种化合物是首次从元宝枫叶中获得。  相似文献   

4.
唐古特白刺叶黄酮类及酚酸类成分的分离鉴定   总被引:13,自引:0,他引:13  
从蒺藜科植物唐古特白刺(NitrariatangutorumBor.)叶中分得8个黄酮类及酚酸类化合物,经理化常数测定和波谱学方法确定了它们的化学结构,分别为:3甲氧基4羟基反式桂皮酸(1)、对羟基反式肉桂酸(2)、3羟基4甲氧基苯甲酸(3)、邻羟基苯甲酸(4)、3,5二甲醚山柰黄素7OβD葡萄糖甙(5)、3甲醚山柰黄素7OβD葡萄糖甙(6)、异鼠李素7OβD葡萄糖甙(7)、异鼠李素3OβD芸香糖甙(8)。以上化合物均为首次从该植物中得到,可以作为白刺属化学分类学的特征性化合物。  相似文献   

5.
亮叶杨桐(石芽茶)中黄酮类成分的研究   总被引:8,自引:0,他引:8  
金静兰  文永新  成桂仁   《广西植物》1985,(3):297-300
从广西产亮叶杨桐(Adinandra nitida Merr.ex H.L.Li)中,分得三种黄酮类成分:Ⅰ、Ⅱ和Ⅲ。经IR、UV(位移诊断)、熔点、混熔点、薄层层析鉴定:Ⅰ为芹菜素(Apigenin);Ⅱ为芹菜素-5-0-α-L-吡喃鼠李糖基(1→4-6~(?)-乙酰基-β-D-吡喃葡萄糖吡;Ⅲ为芹菜素-5-0-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖甙。  相似文献   

6.
7.
苦参中黄酮类化合物成分研究   总被引:1,自引:0,他引:1  
本文对苦参EtOAc提取部分进行了分离,分得3个化合物,根据光谱数据分析鉴定为sophoraflavanone G(Ⅰ),忽布素(Ⅱ),β-谷甾醇(Ⅲ),其中化台物Ⅰ为首次自苦参中分离得到。  相似文献   

8.
三七叶、人参叶和西洋参叶其皂苷类成分相近,但专属性成分各异,皂苷类成分的分布比例也各不相同。本文建立了HPLC-UV法测定上述皂苷成分的方法,经过方法学考察,各种皂苷成分精密度好、加样回收率高,方法可靠。11种皂苷成分总含量顺序为:西洋参叶>人参叶>三七叶;二醇组皂苷成分含量:西洋参叶>三七叶>人参叶;三醇组皂苷成分含量:人参叶>西洋参叶>三七叶。西洋参叶中二醇组皂苷和人参叶中三醇组皂苷含量明显高于其他。西洋参叶中人参皂苷Rb3和Rd的含量之和占11种皂苷成分的60%以上。鉴于其中人参皂苷的高含量,三七叶、人参叶和西洋参叶应该作为皂苷来源得到充分利用;不同的皂苷成分有不同的药理活性,应基于它们的皂苷组成和比例选择性进行研究和开发。  相似文献   

9.
野菊花为菊科(Compositae)植物野菊[Dendranthema indicum (L.) Des Moul.]的头状花序,具有清热解毒和疏风散热等功效.黄酮类化合物为野菊花最主要的有效成分之一,最早从野菊花中分离出的黄酮类化合物为木犀黄酮苷,此后相继得到刺槐素、木犀草素、芹菜素和蒙花苷等黄酮类化合物,以蒙花苷和木犀草素含量最高,其中蒙花苷含量是野菊花药材的主要质量检测指标[1].  相似文献   

10.
Six polymethoxylated flavonoids were isolated from ethyl acetate fraction of ethanol extracts from Vitex rotundifolia Linn. f. fruit, and their structures were identified by NMR and MS methods, and cyt...  相似文献   

11.
正交试验法优选三七茎叶中总黄酮的提取工艺   总被引:2,自引:2,他引:2  
目的:从三七茎叶中提取总黄酮。方法:采用正交试验法研究三七茎叶总黄酮的提取工艺,考察了浸提用水的pH值、浸提温度、浸提时间、浸提次数、液料比五个因素对三七茎叶总黄酮提取率的影响。结果:确立了三七茎叶总黄酮最佳提取条件为:用pH10的稀碱水作溶剂,液料比30:1,浸提温度为80℃,回流提取2次,提取时间为每次40min。  相似文献   

12.
三七叶化学成分的进一步研究   总被引:10,自引:1,他引:10  
从三七叶乙醇提物中分离到16个化合物,分别鉴定为人参皂甙(ginsenoside)Rh2(1),F2(4),Rg3(5),Rg1(7),Rd(8),Re(11),Rb3(13),Rb1(14),Rc(15),七叶胆皂甙(gypenoside)XIII(2),IX(9),XVII(10),三七皂甙(noto-ginsenoside)R1(12),Fa(16),甘草素(liquiritigenin)(3),以及芹糖甘草甙(liquiritin apioside)(6)。其中化合物1,2,3和6首次分离自该植物中,化合物1~8及10~12首次从三七叶中分离到。研究结果进一步证实,三七叶以含原人参二醇型皂甙为主,同时含有微量的原人参三醇型皂甙和黄酮类化合物。  相似文献   

13.
三七环二肽成分和人参内酰胺成分   总被引:5,自引:0,他引:5  
From the roots of Panax notoginseng fourteen cyclodipeptides 1-14 were isolated including one new compound (1),seven new natural compounds (4-10) and six known compounds (2-3,11-14) together with one known other compound 15.The chemical structure of 1 was elucidated as cyclo-(Leu-Thr) based on spectral methods.From the roots of Panax ginseng five known lactams (16-20) includingpyrng lutamic acid were isolated together with butyric diacid,daucosterol and sucrose.The primary binactivity test showed that pyroglutamic acid and its n-butyl derivative have weak Ca^2 antagonistic activity.  相似文献   

14.
三七植物GAPDH基因克隆及序列分析   总被引:12,自引:0,他引:12  
采用改进的异硫氰酸胍法提取高质量三七总RNA,运用RT-PCR方法克隆了三七GAPDH基因的部分序列,长度为627 bp,编码209个氨基酸,为半定量RT-PCR以及Real-time RT-PCR等技术在三七植物研究中的应用提供了条件.氨基酸序列比对结果表明,该序列与拟南芥、烟草、人参的GAPDH氨基酸序列的同源性分别为91%、93%、95%;核苷酸序列的同源性分别为82%、84%、85%.  相似文献   

15.
The biotransformation of the major saponins in Panax notoginseng, including the ginsenosides Rg1, Rh1, Rb1, and Re, by endophytes isolated from P. notoginseng was studied. One hundred and thirty‐six endophytes were isolated and screened for their biotransformational abilities. The results showed that five of the tested endophytes were able to transform these saponins. These five strains were identified based on their ITS or 16S rDNA sequences, which revealed that they belonged to the genera Fusarium, Nodulisporium, Brevundimonas, and Bacillus genera. Ten transformed products were isolated and identified, including a new compound 6‐O‐[α‐L ‐rhamnopyranosyl‐(1→2)‐β‐D ‐glucopyranosyl]‐20‐Oβ‐D ‐glucopyranosyldammarane‐3,6,12,20,24,25‐hexaol ( 3 ), and nine known compounds, compound K ( 1 ), ginsenoside F2 ( 2 ), vinaginsenoside R13 ( 4 ), vinaginsenoside R22 ( 5 ), pseudo‐ginsenoside RT4 ( 6 ), (20S)‐protopanaxatriol ( 7 ), ginsenoside Rg1 ( 8 ), vinaginsenoside R15 ( 9 ), and (20S)‐3‐Oβ‐D ‐glucopyranosyl‐6‐Oβ‐D ‐glucopyranosylprotopanaxatriol ( 10 ). This is the first study on the biotransformation of chemical components in P. notoginseng by endophytes isolated from the same plant.  相似文献   

16.
Panax notoginseng is a highly valuable herb, but root rot disease severely impairs its development and decreases the yield and quality of roots. In this study, a fungal isolate (3A-2-2) was obtained from P. notoginseng seedlings with root rot symptoms and was identified as Plectosphaerella plurivora based on morphological characteristics and molecular analysis. The fungal isolate 3A-2-2 could cause root rot disease and be re-isolated, fulfilling Koch's postulates. This is the first study to report on root rot disease caused by P. plurivora on P. notoginseng in China.  相似文献   

17.
The optimum period of harvesting in cell suspension culture of Panax notoginseng was 30 days. The time course of sap.nih formation proceeded almost in parallel with the cell growth. An appropriate concentration of oligosaccharms from Panax ginseng, precursor fames.l, mannffol and lysozymum which were added into tbe culture broth 10 days before harvesting, all induced saponin biosynthesis significantly. Oligosaecharins at a concentration of 15ppm(it increased 1 fold of saponin yield, and increased 22.7%(of cell growth rate compared with those of the control) and farnesol at 200ppm(it increased 70.5% of sap.nih yield and stimulated cell growth compared with those of the control) were more effective.  相似文献   

18.
The saponin content of Panax notoginseng cell cultures-was 11.14% of dry weight, the saponin yield was 1513.3mg· L-1, and yield of cell cultures was 13.58g dry wt · L-1 per month in fermentation culture, which were all better than those obtained from in suspension culture. Increasing inoculum quantity could obviously increase growth rate, saponin content and yield of cell cultures. An aeration rate of 0.8vvm was optimal for fermentation culture of the cells. The pH value of the culture broth went down from 5.80 to 3.92 gradually and never returned in fermentation culture of P. notoginseng cells.  相似文献   

19.
复合酶解法提取三七皂苷的实验研究   总被引:8,自引:2,他引:6  
以三七提取液中总皂苷的含量和提取物得率为指标,考察了乙醇回流法、渗漉法、纤维素酶解法、果胶酶解法、复合酶解法的优劣,并采用单因素法和四因素(纤维素酶用量、果胶酶用量、酶解温度、乙醇浓度)三水平正交设计法对复合酶解法提取工艺条件进行优选,得到如下较理想的提取工艺条件:纤维素酶用量为15U/g(生药)、果胶酶用量为140U/g(生药),酶解pH值为4.5,酶解温度为50℃,乙醇浓度为80%,提取时间为2.5h。所得三七提取液中总皂苷的含量为12.01%,提取物得率为35.82%。  相似文献   

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