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1.
A critical review of adaptive genetic variation in Atlantic salmon: implications for conservation 总被引:2,自引:0,他引:2
Garcia de Leaniz C Fleming IA Einum S Verspoor E Jordan WC Consuegra S Aubin-Horth N Lajus D Letcher BH Youngson AF Webb JH Vøllestad LA Villanueva B Ferguson A Quinn TP 《Biological reviews of the Cambridge Philosophical Society》2007,82(2):173-211
Here we critically review the scale and extent of adaptive genetic variation in Atlantic salmon (Salmo salar L.), an important model system in evolutionary and conservation biology that provides fundamental insights into population persistence, adaptive response and the effects of anthropogenic change. We consider the process of adaptation as the end product of natural selection, one that can best be viewed as the degree of matching between phenotype and environment. We recognise three potential sources of adaptive variation: heritable variation in phenotypic traits related to fitness, variation at the molecular level in genes influenced by selection, and variation in the way genes interact with the environment to produce phenotypes of varying plasticity. Of all phenotypic traits examined, variation in body size (or in correlated characters such as growth rates, age of seaward migration or age at sexual maturity) generally shows the highest heritability, as well as a strong effect on fitness. Thus, body size in Atlantic salmon tends to be positively correlated with freshwater and marine survival, as well as with fecundity, egg size, reproductive success, and offspring survival. By contrast, the fitness implications of variation in behavioural traits such as aggression, sheltering behaviour, or timing of migration are largely unknown. The adaptive significance of molecular variation in salmonids is also scant and largely circumstantial, despite extensive molecular screening on these species. Adaptive variation can result in local adaptations (LA) when, among other necessary conditions, populations live in patchy environments, exchange few or no migrants, and are subjected to differential selective pressures. Evidence for LA in Atlantic salmon is indirect and comes mostly from ecological correlates in fitness-related traits, the failure of many translocations, the poor performance of domesticated stocks, results of a few common-garden experiments (where different populations were raised in a common environment in an attempt to dissociate heritable from environmentally induced phenotypic variation), and the pattern of inherited resistance to some parasites and diseases. Genotype x environment interactions occurr for many fitness traits, suggesting that LA might be important. However, the scale and extent of adaptive variation remains poorly understood and probably varies, depending on habitat heterogeneity, environmental stability and the relative roles of selection and drift. As maladaptation often results from phenotype-environment mismatch, we argue that acting as if populations are not locally adapted carries a much greater risk of mismanagement than acting under the assumption for local adaptations when there are none. As such, an evolutionary approach to salmon conservation is required, aimed at maintaining the conditions necessary for natural selection to operate most efficiently and unhindered. This may require minimising alterations to native genotypes and habitats to which populations have likely become adapted, but also allowing for population size to reach or extend beyond carrying capacity to encourage competition and other sources of natural mortality. 相似文献
2.
Recent anthropogenic habitat perturbationsrelated to the unsustainable development of thetwo areas of distribution of Androcymbiumgramineum in Almería (South of Spain) andthe Atlantic coast of Morocco are outweighingthe intrinsic biological survival assets ofthis narrow Ibero-Moroccan endemic. We usedpopulation genetic data on 18 isozyme loci for13 populations to design a comprehensivesampling strategy for ex-situ conservation thatstraddles the results of independenttheoretical developments and the indications ofseveral genetic polymorphism parameters.Regressions based on the probabilities of lossestimate that sampling one population would beinsufficient to represent the variationattributable to the rare alleles found in thepopulations surveyed from Almería orMorocco and suggest that the relationshipbetween the number of populations sampled ineither area should conform to the proportion0.6:0.4, respectively. The estimate of thenumber of populations that need be targeted torepresent 99% of the genetic variationdetected indicates that it would be necessaryto sample eight populations intensively. Fivepopulations from Almería and three fromMorocco were selected through the ranked valuesof the average number of alleles per locus andthe expected heterozygosity on the grounds thatthese parameters provide unambiguousindications of polymorphism that, in the caseof heterozygosity, are less likely to beinfluenced by sampling error. Spatialautocorrelation surveys in the largestpopulation known indicate that seeds should becollected at a minimum separation of 15 m toavoid the sampling of close relatives. 相似文献
3.
The Evolutionarily Significant Unit (ESU) was conceptualized in 1986 as a conservation unit below the species level, theoretically
applicable to a wide range of taxa. The concept has gained support, and various definitions or criteria, some of which are
inconsistent with each other, have since been proposed. Recent critiques of the ESU have pointed out the dominance of definitions
biased to the identification of long-term isolation or neutral genetic variation, which has largely ignored the adaptive components.
We present here the validity of such claims and show how the ESU definitions have actually been applied in research. We surveyed
scientific journals for original papers supporting ESU designations and determined who among the proponents of ESU definitions
have gained wider support. Our results indicate that indeed there are inconsistencies with the original concept and with the
existing definitions. Although the original concept recommended both ecological and genetic data as the basis for identification
of ESUs, which reflect true evolutionary variation, recent definitions have become biased to either neutral genetic variation
or adaptive variation. The definition which uses genetic data to assess neutral genetic variation (long-term isolation) has
gained major support, and therefore validates the earlier claims. To bridge the gap between the original concept and the practical
application, we propose the use of partial ESU and full ESU designations. The application of full ESU should be limited solely
to when both information about neutral genetic variation and adaptive variation are available. In other cases, in which only
a part of the variation is examined, we should use the term partial ESU (e.g., molecular-based ESU) and continue to investigate
focal populations from other aspects of variations to designate full ESU. 相似文献
4.
Pacheco-Villalobos D Hardtke CS 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2012,367(1595):1552-1558
Root system architecture is a trait that displays considerable plasticity because of its sensitivity to environmental stimuli. Nevertheless, to a significant degree it is genetically constrained as suggested by surveys of its natural genetic variation. A few regulators of root system architecture have been isolated as quantitative trait loci through the natural variation approach in the dicotyledon model, Arabidopsis. This provides proof of principle that allelic variation for root system architecture traits exists, is genetically tractable, and might be exploited for crop breeding. Beyond Arabidopsis, Brachypodium could serve as both a credible and experimentally accessible model for root system architecture variation in monocotyledons, as suggested by first glimpses of the different root morphologies of Brachypodium accessions. Whether a direct knowledge transfer gained from molecular model system studies will work in practice remains unclear however, because of a lack of comprehensive understanding of root system physiology in the native context. For instance, apart from a few notable exceptions, the adaptive value of genetic variation in root system modulators is unknown. Future studies should thus aim at comprehensive characterization of the role of genetic players in root system architecture variation by taking into account the native environmental conditions, in particular soil characteristics. 相似文献
5.
6.
Population genomics of marine fishes: identifying adaptive variation in space and time 总被引:1,自引:0,他引:1
EINAR E. NIELSEN JAKOB HEMMER-HANSEN PETER FOGED LARSEN† DORTE BEKKEVOLD 《Molecular ecology》2009,18(15):3128-3150
Studies of adaptive evolution have experienced a recent revival in population genetics of natural populations and there is currently much focus on identifying genomic signatures of selection in space and time. Insights into local adaptation, adaptive response to global change and evolutionary consequences of selective harvesting can be generated through population genomics studies, allowing the separation of the effects invoked by neutral processes (drift-migration) from those due to selection. Such knowledge is important not only for improving our basic understanding of natural as well as human-induced evolutionary processes, but also for predicting future trajectories of biodiversity and for setting conservation priorities. Marine fishes possess a number of features rendering them well suited for providing general insights into adaptive genomic evolution in natural populations. These include well-described population structures, substantial and rapidly developing genomic resources and abundant archived samples enabling temporal studies. Furthermore, superior possibilities for conducting large-scale experiments under controlled conditions, due to the economic resources provided by the large and growing aquaculture industry, hold great promise for utilizing recent technological developments. Here, we review achievements in marine fish genomics to date and highlight potential avenues for future research, which will provide both general insights into evolution in high gene flow species, as well as specific knowledge which can lead to improved management of marine organisms. 相似文献
7.
John R. Candy Nathan R. Campbell Matthew H. Grinnell Terry D. Beacham Wesley A. Larson Shawn R. Narum 《Molecular ecology resources》2015,15(6):1421-1434
Twelve eulachon (Thaleichthys pacificus, Osmeridae) populations ranging from Cook Inlet, Alaska and along the west coast of North America to the Columbia River were examined by restriction‐site‐associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high geneflow species. A total of 4104 single‐nucleotide polymorphisms (SNPs) were discovered across the genome, with 193 putatively adaptive SNPs as determined by FST outlier tests. Estimates of population structure in eulachon with the putatively adaptive SNPs were similar, but provided greater resolution of stocks compared with a putatively neutral panel of 3911 SNPs or previous estimates with 14 microsatellites. A cline of increasing measures of genetic diversity from south to north was found in the adaptive panel, but not in the neutral markers (SNPs or microsatellites). This may indicate divergent selective pressures in differing freshwater and marine environments between regional eulachon populations and that these adaptive diversity patterns not seen with neutral markers could be a consideration when determining genetic boundaries for conservation purposes. Estimates of effective population size (Ne) were similar with the neutral SNP panel and microsatellites and may be utilized to monitor population status for eulachon where census sizes are difficult to obtain. Greater differentiation with the panel of putatively adaptive SNPs provided higher individual assignment accuracy compared to the neutral panel or microsatellites for stock identification purposes. This study presents the first SNPs that have been developed for eulachon, and analyses with these markers highlighted the importance of integrating genome‐wide neutral and adaptive genetic variation for the applications of conservation and management. 相似文献
8.
Silvio Schueler Wolfgang Falk Jarkko Koskela François Lefèvre Michele Bozzano Jason Hubert Hojka Kraigher Roman Longauer Ditte C. Olrik 《Global Change Biology》2014,20(5):1498-1511
A transnational network of genetic conservation units for forest trees was recently documented in Europe aiming at the conservation of evolutionary processes and the adaptive potential of natural or man‐made tree populations. In this study, we quantified the vulnerability of individual conservation units and the whole network to climate change using climate favourability models and the estimated velocity of climate change. Compared to the overall climate niche of the analysed target species populations at the warm and dry end of the species niche are underrepresented in the network. However, by 2100, target species in 33–65 % of conservation units, mostly located in southern Europe, will be at the limit or outside the species' current climatic niche as demonstrated by favourabilities below required model sensitivities of 95%. The highest average decrease in favourabilities throughout the network can be expected for coniferous trees although they are mainly occurring within units in mountainous landscapes for which we estimated lower velocities of change. Generally, the species‐specific estimates of favourabilities showed only low correlations to the velocity of climate change in individual units, indicating that both vulnerability measures should be considered for climate risk analysis. The variation in favourabilities among target species within the same conservation units is expected to increase with climate change and will likely require a prioritization among co‐occurring species. The present results suggest that there is a strong need to intensify monitoring efforts and to develop additional conservation measures for populations in the most vulnerable units. Also, our results call for continued transnational actions for genetic conservation of European forest trees, including the establishment of dynamic conservation populations outside the current species distribution ranges within European assisted migration schemes. 相似文献
9.
Benjamin Dauphin Rafael O. Wüest Sabine Brodbeck Stefan Zoller Martin C. Fischer Rolf Holderegger Felix Gugerli Christian Rellstab 《Molecular ecology》2020,29(11):1972-1989
It is generally accepted that the spatial distribution of neutral genetic diversity within a species’ native range mostly depends on effective population size, demographic history, and geographic position. However, it is unclear how genetic diversity at adaptive loci correlates with geographic peripherality or with habitat suitability within the ecological niche. Using exome‐wide genomic data and distribution maps of the Alpine range, we first tested whether geographic peripherality correlates with four measures of population genetic diversity at > 17,000 SNP loci in 24 Alpine populations (480 individuals) of Swiss stone pine (Pinus cembra) from Switzerland. To distinguish between neutral and adaptive SNP sets, we used four approaches (two gene diversity estimates, FST outlier test, and environmental association analysis) that search for signatures of selection. Second, we established ecological niche models for P. cembra in the study range and investigated how habitat suitability correlates with genetic diversity at neutral and adaptive loci. All estimates of neutral genetic diversity decreased with geographic peripherality, but were uncorrelated with habitat suitability. However, heterozygosity (He) at adaptive loci based on Tajima's D declined significantly with increasingly suitable conditions. No other diversity estimates at adaptive loci were correlated with habitat suitability. Our findings suggest that populations at the edge of a species' geographic distribution harbour limited neutral genetic diversity due to demographic properties. Moreover, we argue that populations from suitable habitats went through strong selection processes, are thus well adapted to local conditions, and therefore exhibit reduced genetic diversity at adaptive loci compared to populations at niche margins. 相似文献
10.
Utility of RAPD markers in identifying genetic linkages to genes of economic interest in peach 总被引:7,自引:0,他引:7
M. L. Warburton V. L. Becerra-Velásquez J. C. Goffreda F. A. Bliss 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(5-6):920-925
The identification of molecular markers linked to economically important traits for use in crop improvement is very important in long-lived perennial species. Three-hundred-and-sixty RAPD primers were used with bulked segregant analysis to identify markers linked to loci of specific interest in peach [(Prunus persica) L. Batch] and peach x almond [(Prunus dulcis) Batch] crosses. The traits analyzed included flesh color, adhesion, and texture; pollen fertility; plant stature; and three isozyme loci. The Mendelian behavior of the RAPD loci was established, and RAPD markers were mapped relative to the loci controlling flesh color, adhesion, and texture, and the isozyme loci Mdh-1, 6Pgd-2 and Aat-1, as well as the existing RFLP genetic linkage map constructed previously using a peach x almond F2 population. This technique has facilitated rapid identification of RAPD and RFLP markers that are linked to the traits under study. Loci controlling these traits mapped predominantly to linkage groups 2 and 3 of the peach genetic linkage map. Linkages to genes with both dominant and co-dominant alleles were identified, but linkages to dominant genes were more difficult to find. In several crosses, RAPD marker bands proved to be allelic. One co-dominant RAPD formed a heteroduplex band in heterozygous individuals and in mixtures of alternate homozygotes. The Mendelian behavior of the RAPD loci studied was established and the results suggest that RAPD markers will be useful for plant improvement in peach. 相似文献
11.
Landscape genetics, an emerging field integrating landscape ecology and population genetics, has great potential to influence our understanding of habitat connectivity and distribution of organisms. Whereas typical population genetics studies summarize gene flow as pairwise measures between sampling localities, landscape characteristics that influence population genetic connectivity are often continuously distributed in space. Thus, there are currently gaps in both the ability to analyze genotypic data in a continuous spatial context and our knowledge of expected of landscape genetic structure under varying conditions. We present a framework for generating continuous “genetic surfaces”, evaluate their statistical properties, and quantify statistical behavior of landscape genetic structure in a simple landscape. We simulated microsatellite genotypes under varying parameters (time since vicariance, migration, effective population size) and used ancestry (q) values from STRUCTURE to interpolate a genetic surface. Using a spatially adjusted Pearson's correlation coefficient to test the significance of landscape variable(s) on genetic structure we were able to detect landscape genetic structure on a contemporary time scale (≥5 generations post vicariance, migration probability ≤0.10) even when population differentiation was minimal (FST≥0.00015). We show that genetic variation can be significantly correlated with geographic distance even when genetic structure is due to landscape variable(s), demonstrating the importance of testing landscape influence on genetic structure. Finally, we apply genetic surfacing to analyze an empirical dataset of black bears from northern Idaho USA. We find black bear genetic variation is a function of distance (autocorrelation) and habitat patch (spatial dependency), consistent with previous results indicating genetic variation was influenced by landscape by resistance. These results suggest genetic surfaces can be used to test competing hypotheses of the influence of landscape characteristics on genetic structure without delineation of categorical groups. 相似文献
12.
Summary The allelic frequencies of the plasma enzymes transferrin (Tf) and leucine aminopeptidase (LAP) have been shown previously to correlate with population density, population growth rate and mortality in Microtus ochrogaster. Such changes in allozymic frequencies of Tf and LAP have been used by others as evidence supporting the Chitty/Krebs genetic-behavioral hypothesis of population growth. In this study, prairie voles were captured in midwinter and in midsummer, and brought to the lab where their allozymes of Tf and LAP were determined. The voles were then placed in controlled environments approximating some of the conditions of other seasons of the year, and their allozymes were redetermined in subsequent weeks. Changes in allozymes occurred in all experiments. Animals subected to opposite seasons (i.e. captured in winter and subjected to summer conditions, and captured in summer and subjected to winter environments) had the highest percentage of changes in electromorphs. A review of the literature revealed a plethora of evidence that Tf and LAP proteins could change electromorphs on starch gels in response to differences in blood chemistry and presumably to physiological state influenced by diet, reproductive state, and disease. Thus, since it appears that electromorphs of Tf and LAP proteins can change in voles subjected to different environments, and that these proteins may change in electronegativity in response to the physiological state of the animals, observed changes in allelic frequencies in populations of voles may be of limited value as evidence for the proposed genetic-behavioral hypothesis explaining population cycles. 相似文献
13.
Marsden CD Woodroffe R Mills MG McNutt JW Creel S Groom R Emmanuel M Cleaveland S Kat P Rasmussen GS Ginsberg J Lines R André JM Begg C Wayne RK Mable BK 《Molecular ecology》2012,21(6):1379-1393
Deciphering patterns of genetic variation within a species is essential for understanding population structure, local adaptation and differences in diversity between populations. Whilst neutrally evolving genetic markers can be used to elucidate demographic processes and genetic structure, they are not subject to selection and therefore are not informative about patterns of adaptive variation. As such, assessments of pertinent adaptive loci, such as the immunity genes of the major histocompatibility complex (MHC), are increasingly being incorporated into genetic studies. In this study, we combined neutral (microsatellite, mtDNA) and adaptive (MHC class II DLA‐DRB1 locus) markers to elucidate the factors influencing patterns of genetic variation in the African wild dog (Lycaon pictus); an endangered canid that has suffered extensive declines in distribution and abundance. Our genetic analyses found all extant wild dog populations to be relatively small (Ne < 30). Furthermore, through coalescent modelling, we detected a genetic signature of a recent and substantial demographic decline, which correlates with human expansion, but contrasts with findings in some other African mammals. We found strong structuring of wild dog populations, indicating the negative influence of extensive habitat fragmentation and loss of gene flow between habitat patches. Across populations, we found that the spatial and temporal structure of microsatellite diversity and MHC diversity were correlated and strongly influenced by demographic stability and population size, indicating the effects of genetic drift in these small populations. Despite this correlation, we detected signatures of selection at the MHC, implying that selection has not been completely overwhelmed by genetic drift. 相似文献
14.
Levsen ND Crawford DJ Archibald JK Santos-Geurra A Mort ME 《American journal of botany》2008,95(11):1466-1474
Accurate determination of patterns of genetic variation provides a powerful inferential tool for studies of evolution and conservation. For more than 30 years, enzyme electrophoresis was the preferred method for elucidating these patterns. As a result, evolutionary geneticists have acquired considerable understanding of the relationship between patterns of allozyme variation and aspects of evolutionary process. Myriad molecular markers and statistical analyses have since emerged, enabling improved estimates of patterns of genetic diversity. With these advances, there is a need to evaluate results obtained with different markers and analytical methods. We present a comparative study of gene statistic estimates (F(ST), G(ST), F(IS), H(S), and H(T)) calculated from an intersimple sequence repeat (ISSR) and an allozyme data set derived from the same populations using both standard and Bayesian statistical approaches. Significant differences were found between estimates, owing to the effects of marker and analysis type. Most notably, F(ST) estimates for codominant data differ between Bayesian and standard approaches. Levels of statistical significance are greatly affected by methodology and, in some cases, are not associated with similar levels of biological significance. Our results suggest that caution should be used in equating or comparing results obtained using different markers and/or methods of analysis. 相似文献
15.
da Costa-Ribeiro MC Lourenço-de-Oliveira R Failloux AB 《Memórias do Instituto Oswaldo Cruz》2006,101(8):917-921
Aedes aegypti populations from five districts in Rio de Janeiro were analyzed using five microsatellites and six isoenzyme markers, to assess the amount of variation and patterns of gene flow at local levels. Microsatellite loci were polymorphic enough to detect genetic differentiation of populations collected at small geographic scales (e.g. within a city). Ae. aegypti populations were highly differentiated as well in the city center as in the outskirt. Thus, dengue virus propagation by mosquitoes could be as efficient in the urban area as in the outskirt of Rio de Janeiro, the main entry point of dengue in Brazil. 相似文献
16.
David Weinshenker Malania M. Wilson Katherine M. Williams Jay M. Weiss Neil E. Lamb Simon N. Twigger 《Mammalian genome》2005,16(10):784-791
Microsatellite length polymorphisms are useful for the mapping of heritable traits in rats. Over 4000 such microsatellites
have been characterized for 48 inbred rat strains and used successfully to map phenotypes that differ between strains. At
present, however, it is difficult to use this microsatellite database for mapping phenotypes in selectively bred rats of unknown
genotype derived from outbred populations because it is not immediately obvious which markers might differ between strains
and be informative. We predicted that markers represented by many alleles among the known inbred rat strains would also be
most likely to differ between selectively bred strains derived from outbred populations. Here we describe the development
and successful application of a new genotyping tool (HUMMER) that assigns “heterozygosity” (Het) and “uncertainty” (Unc) scores
to each microsatellite marker that corresponds to its degree of heterozygosity among the 48 genotyped inbred strains. We tested
the efficiency of HUMMER on two rat strains that were selectively bred from an outbred Sprague-Dawley stock for either high
or low activity in the forced swim test (SwHi rats and SwLo rats, respectively). We found that the markers with high Het and
Unc scores allowed the efficient selection of markers that differed between SwHi and SwLo rats, while markers with low Het
and Unc scores typically identified markers that did not differ between strains. Thus, picking markers based on Het and Unc
scores is a valuable method for identifying informative microsatellite markers in selectively bred rodent strains derived
from outbred populations. 相似文献
17.
It is very important to be able to distinguish between selectively significant genetic variability and selectively-neutral one for quantitative analysis of genetic differentiation in human (and any other) populations. The key to the problem is to determine a start-point for detection of neutral genetic variability, which will help to establish alignment of adaptive and neutral forces operating in genetic differentiation. The purpose of this work is to adduce proofs in favour of mean Fst value for a sample of gene loci as of the start-point for measuring neutrality level of genetic differentiation. These proofs came from various demographic and onomastic characteristics of ethnic groups as well as from genetic chronology of ethnic history which is in good concordance with actual historical chronology of ethnic groups. Once the start-point for testing neutrality is determined, it becomes possible to reveal the selective pressure to which various human genes are undergone and to elucidate adaptive structure of mankind's genetic pool. It was shown that only 15 alleles from 49 belonging to 20 polymorphic loci can be considered selectively neutral. 相似文献
18.
Patrik Rdin‐Mrch Emilien Luquet Yvonne Meyer‐Lucht Alex Richter‐Boix Jacob Hglund Anssi Laurila 《Molecular ecology》2019,28(12):2996-3011
Stochastic effects from demographic processes and selection are expected to shape the distribution of genetic variation in spatially heterogeneous environments. As the amount of genetic variation is central for long‐term persistence of populations, understanding how these processes affect variation over large‐scale geographical gradients is pivotal. We investigated the distribution of neutral and putatively adaptive genetic variation, and reconstructed demographic history in the moor frog (Rana arvalis) using 136 individuals from 15 populations along a 1,700‐km latitudinal gradient from northern Germany to northern Sweden. Using double digest restriction‐site associated DNA sequencing we obtained 27,590 single nucleotide polymorphisms (SNPs), and identified differentiation outliers and SNPs associated with growing season length. The populations grouped into a southern and a northern cluster, representing two phylogeographical lineages from different post‐glacial colonization routes. Hybrid index estimation and demographic model selection showed strong support for a southern and northern lineage and evidence of gene flow between regions located on each side of a contact zone. However, patterns of past gene flow over the contact zone differed between neutral and putatively adaptive SNPs. While neutral nucleotide diversity was higher along the southern than the northern part of the gradient, nucleotide diversity in differentiation outliers showed the opposite pattern, suggesting differences in the relative strength of selection and drift along the gradient. Variation associated with growing season length decreased with latitude along the southern part of the gradient, but not along the northern part where variation was lower, suggesting stronger climate‐mediated selection in the north. Outlier SNPs included loci involved in immunity and developmental processes. 相似文献
19.
Microsatellite markers were developed as an identification tool and for analysis of the genetic variation in the pathogens Cronartium flaccidum and Peridermium pini, causing Scots pine blister rust in Pinus spp. Six reference aeciospore samples from Finland were used to examine genetic differences between the two pathogens. Genetic variation within and between 27 lesions on Scots pines from seven locations in Sweden was also investigated. Aeciospores were collected from single aecia within the lesions. Reference samples from P. pini were homozygous for all seven microsatellite loci investigated, while the three C.?flaccidum samples contained heterozygous loci. These results confirm previous studies, where homozygous aeciospores were indicated to be characteristic for P. pini. The majority of aeciospores had two nuclei in both heterozygotic and homozygotic samples. Five of the Swedish lesions contained only homozygotic aecia, while the aecia in the remaining 22 lesions were heterozygotic. All lesions with homozygotic aecia contained only one single multilocus genotype, while many of the lesions with heterozygotic aecia contained several genotypes. The latter indicates the occurrence of multiple matings within a lesion between the resident spermogonia and alien fertilizing spermatia. 相似文献
20.
We report the results of a novel experiment, in which genetically pure male red junglefowl Gallus gallus (Richardson strain) were deliberately crossed with domestic female chickens to create contaminated lines of known purity, reaching as high as 93.75%. Phenotypic characters generally used as indicators of purity (reduced or absent female comb, male eclipse plumage, etc.) all appeared to at least some extent in domestically contaminated progeny and moreso in successively more pure generations of the experiment, suggesting that such phenotypic characters may have little, if any, utility in characterizing red junglefowl stocks as to their genetic purity. 相似文献