Traces and related chemical changes in a Late Ordovician paleosol,Glossifungites ichnofacies,southern Appalachians,USA

Traces interpreted as Skolithos sp. in a Glossifungites ichnofacies occur in the upper part of the Beans Gap Claystone paleosol, in association with a Late Ordovician marine erosional surface. Paleosol color and whole‐rock chemistry are distinctly different in the vicinity of burrows, which are enveloped by olive gray mottles, 1 to 10 cm in diameter, in which total iron (measured as Fe₂O₃) is 30% lower than in grayish red, “unaltered”; claystone.

Post‐pedogenic bioturbation increased permeability locally and permitted the introduction of marine fluids and coarser sediment into the top of the paleosol. Anaerobic microbial decay of organic matter in the burrows and surrounding claystone permitted reduction (and mobilization) of formerly oxidized iron, which then combined with marine‐derived sulfate to form pyrite, both within burrows and along oxidation‐reduction fronts in the claystone proximal to burrows. Pyrite was later converted to hematite as a result of outcrop‐related oxidation by meteoric water.

Paleosols formed in coastal marine settings are especially susceptible to early diagenetic alteration related to marine hydromorphism. Differentiating these ichnologic and diagenetic effects from true pedogenic ones is essential when interpreting paleosols in paralic sequences.     

Carbonate facies dominated by syndepositional cements: a key component of Middle Triassic platforms. The Marmolada case history (Dolomites, Italy)

Summary This article deals with the discussion of the role of the syndepositional cementation for the growth of the Middle Triassic pre-volcanic carbonate platforms of the Dolomites (Southern Alps, Northern Italy). The study is concentrated on the Marmolada Buildup, which escaped the facies destroying dolomitization which affected many surrounding platforms. The investigations took place within an almost isochronous uppermost Anisian palcogeographic transect, ranging from the platform-top to the margin and the upper slope. Methods used include geological mapping, sedimentological and paleontological studies, evaluation of the microfacies, as well as SEM and EDS epifluorescence analyses. The well bedded platform-top succession consists of intra-bioclast calcarenites and calcirudites, interbedded with subordinate boundstones, and organized in shallowing upward, meter scale depositional cycles, sometimes capped by subaerial surfaces. The platform margin belt is rich in boundstones and lacks a primary framework formed by organisms; metazoan skeletons form less then 5% of the rock volume. The outer margin and the uppermost slope are characterized by decimeter-scale boundstone blocks, coated and linked to each other by huge amounts of radiaxial fibrous calcite cements, arranged in concentric crusts. These cements (“evinospongiac”) represent the main component of the margin and upper slope facies. Epifluorescence analyses suggest the existence of abundant organic residual matter associated not only with the bioclasts and peloids, but also with the syndepositional cements. Organic matter likely played a significant role in carbonate cementation and was a key factor for the early lithification of the platform as well as for the sediment production. Minor element microanalyses reveal an uniform Mg content in different calcite types (2–4 Mole % MgCO₃), independently from the primary nature of the components. Late diagenetic sparry calcites exhibit similar Mg values but no iron. These data point to a homogenization of minor element distribution, probably associated with a slow but long-lasting semi-closed fluid circulation, possibly related with the Neogene uplifting of the Dolomite Mountains.     

Burrows of the Semi-Terrestrial Crab Ucides cordatus Enhance CO2 Release in a North Brazilian Mangrove Forest

Ucides cordatus is an abundant mangrove crab in Brazil constructing burrows of up to 2 m depth. Sediment around burrows may oxidize during low tides. This increase in sediment-air contact area may enhance carbon degradation processes. We hypothesized that 1) the sediment CO₂ efflux rate is greater with burrows than without and 2) the reduction potential in radial profiles in the sediment surrounding the burrows decreases gradually, until approximating non-bioturbated conditions. Sampling was conducted during the North Brazilian wet season at neap tides. CO₂ efflux rates of inhabited burrows and plain sediment were measured with a CO₂/H₂O gas analyzer connected to a respiration chamber. Sediment redox potential, pH and temperature were measured in the sediment surrounding the burrows at horizontal distances of 2, 5, 8 and 15 cm at four sediment depths (1, 10, 30 and 50 cm) and rH values were calculated. Sediment cores (50 cm length) were taken to measure the same parameters for plain sediment. CO₂ efflux rates of plain sediment and individual crab burrows with entrance diameters of 7 cm were 0.7–1.3 µmol m⁻² s⁻¹ and 0.2–0.4 µmol burrows⁻¹ s⁻¹, respectively. CO₂ released from a Rhizophora mangle dominated forest with an average of 1.7 U. cordatus burrows⁻¹ m⁻² yielded 1.0–1.7 µmol m⁻² s⁻¹, depending on the month and burrow entrance diameter. Laboratory experiments revealed that 20–60% of the CO₂ released by burrows originated from crab respiration. Temporal changes in the reduction potential in the sediment surrounding the burrows did not influence the CO₂ release from burrows. More oxidized conditions of plain sediment over time may explain the increase in CO₂ release until the end of the wet season. CO₂ released by U. cordatus and their burrows may be a significant pathway of CO₂ export from mangrove sediments and should be considered in mangrove carbon budget estimates.     

Effects of the burrowing mud shrimp,Upogebia yokoyai,on carbon flow and microbial activity on a tidal flat

Mud shrimps, Upogebia spp., are major constituents of macrobenthic communities in tidal flats in Japan. The impact of Upogebia yokoyai on carbon flow on tidal flats was examined by comparing CO₂ emission rates from plots with and without burrows in the Kurose River estuary, Japan. In situ CO₂ emission rates from plots with burrows were significantly higher than from those without. Laboratory measurements using sediment core samples that excluded respiration of macrobenthic organisms showed similar trends. Although there were no significant differences in grain size distribution, water content, or ignition loss between the sediment cores with and without burrows, oxidation–reduction potential was significantly higher in sediment cores with burrows. Analysis of phospholipid fatty acids (PLFA) indicated that microbial biomass and community structure did not differ significantly between cores with and without burrows. However, microbial respiration activity, as indicated by CO₂ emission rates per total PLFA content, was significantly higher in sediment cores with burrows than in those without. Our results indicate that burrows of U. yokoyai change the physicochemical conditions and increase microbial activity in the sediment, significantly affecting carbon flow in the tidal flat.     

Conceptual models for burrow-related, selective dolomitization with textural and isotopic evidence from the Tyndall Stone, Canada

The formation of dolomite is generally explained using models that reflect larger‐scale processes that describe the relationship between the supply and transport of Mg, and geochemical conditions that are amenable to the formation of dolomite. However, heterogeneities in the substrate, such as those made by bioturbating infauna, may play a more important role in dolomitization than has been previously considered. Burrow‐facilitated dolomitization is evident in the Ordovician Tyndall Stone (Red River Group, Selkirk Formation) of central Canada. The diagenetic fabrics present are attributed to dolomitizing fluids that both flowed through and evolved within burrow networks. Petrographic analysis suggests that two phases of dolomite formation took place. The first formed a fine‐grained, fabric‐destructive type that probably accompanied early burial; the second is a fine‐ to medium‐grained, locally sucrosic dolomite that is interpreted to have precipitated during later burial. Isotopic analysis supports the proposed paragenetic history: (1) an apparent linking of the stable isotopes ¹³C and ¹⁸O strongly suggests that the micrite matrix formed during very early diagenesis and was derived from seawater; (2) the initial phase of dolomitization is potentially microbially mediated, as evidenced by the enrichment of ¹³C; and (3) isotopic values for the second generation of dolomite reflect the mixing of ground water and resorbed early dolomite. This paper conceptualizes the physical and chemical conditions required for the formation of dolomite in association with burrow fabrics. The proposed model reveals a composite of biological and inorganic reactions that demonstrates the interdependence of sediment fabric, organic content and microbial interactions in the development of burrow‐mottled dolomitic limestone. It is suggested that where burrow‐associated dolomite occurs, it is most likely to develop in two stages: first, the byproducts of the degradation of organic material in burrows locally increase the permeability and porosity around burrow fabrics in shallow diagenetic depositional environments; and, second, the passing of burrowed media into deeper dysaerobic sediment is accompanied by the establishment of fermenting micro‐organisms whose byproducts mediate dolomitization.     

Microbial activities in the burrow environment of the potamal mayfly Ephoron virgo

1. The impact of burrowing larvae of Ephoron virgo (Ephemeroptera, Polymitarcidae) on sediment microbiology has not been previously investigated because of difficulties in sampling the sediment of large rivers under in situ conditions. Therefore, we conducted experiments in the on‐ship Ecological Rhine Station of the University of Cologne (Germany), in which ambient conditions of the River Rhine can be closely mimicked. 2. In two consecutive seasons, experimental flow channels were stocked with Ephoron larvae and continuously supplied with water taken directly from the River Rhine. Sediment from the immediate vicinity of Ephoron burrows (i.e. U‐shaped cavities reaching 10–80 mm deep into the sediment) and bulk sediment samples were analysed for (i) particulate organic matter content, (ii) microscale in situ distribution of O₂, NO, and NH, and (iii) potential activities of exoenzymes. 3. Sediment surrounding the Ephoron burrows had markedly higher organic matter contents and exoenzyme activities compared with the bulk sediment. Microsensor measurements demonstrated that local O₂ and NO penetration into the sediment were greatly enhanced by larval ventilation behaviour. Volumetric O₂ and NO turnover rates that were calculated from steady state concentration profiles measured directly in the burrow lining were considerably higher than at the sediment surface. 4. In the sediment of the fast flowing River Rhine Ephoron burrows are preferential sites of organic matter accumulation and dissolved oxidant penetration. Our data suggest that the burrows are surrounded by a highly active microbial community that responds to the inputs from the water column with elevated O₂ and NO turnover, and release of exoenzymes into the sediment pore water. Especially during periods of mass occurrence, the larvae of E. virgo may thus significantly contribute (i) to the ecological connection between the water column and the sediment and (ii) to biogeochemical processing of organic matter in the riverbed.     

A diagenetic control on the Early Triassic Smithian–Spathian carbon isotopic excursions recorded in the marine settings of the Thaynes Group (Utah,USA)

In the aftermath of the end‐Permian mass extinction, Early Triassic sediments record some of the largest Phanerozoic carbon isotopic excursions. Among them, a global Smithian‐negative carbonate carbon isotope excursion has been identified, followed by an abrupt increase across the Smithian–Spathian boundary (SSB; ~250.8 Myr ago). This chemostratigraphic evolution is associated with palaeontological evidence that indicate a major collapse of terrestrial and marine ecosystems during the Late Smithian. It is commonly assumed that Smithian and Spathian isotopic variations are intimately linked to major perturbations in the exogenic carbon reservoir. We present paired carbon isotopes measurements from the Thaynes Group (Utah, USA) to evaluate the extent to which the Early Triassic isotopic perturbations reflect changes in the exogenic carbon cycle. The δ¹³C_carb variations obtained here reproduce the known Smithian δ¹³C_carb‐negative excursion. However, the δ¹³C signal of the bulk organic matter is invariant across the SSB and variations in the δ³⁴S signal of sedimentary sulphides are interpreted here to reflect the intensity of sediment remobilization. We argue that Middle to Late Smithian δ¹³C_carb signal in the shallow marine environments of the Thaynes Group does not reflect secular evolution of the exogenic carbon cycle but rather physicochemical conditions at the sediment–water interface leading to authigenic carbonate formation during early diagenetic processes.     

Habitat and sediment preferences ofAxarus festivus larvae

During fall draw-down of a reservoir in northeast Kansas it was observed that larvae ofAxarus festivus were restricted to highly weathered Pennsylvanian Shale outcrops and surrounding coarse sediments with high-clay content derived from erosion of the shale outcroppings. Larvae constructed burrows into the outcrops and eroded coarse sediments, which they used to filter feed by setting up currents through the burrows. Burrows were widely distributed over the outcrops, with average densities ranging from 372–2,351 burrows m^–2. However, closer inspection revealed that burrows were more common at apices of individual shale strata, where weathering of the outcrop was most advanced. Here burrows were more uniformly distributed and densities ranged to 4,166 burrows m^–2. 73% of burrows contained larvae. Burrows were generally U-shaped, and averaged 1.8 mm in diameter and 42 mm in total length. Laboratory experiments revealed that 4th instar larvae removed from burrows could construct new burrows in weathered shale, but preferentially used old empty burrows if available. When given choices among alternative sediment combinations of sandvs. finely-ground shale, sandvs. coarsely-ground shale, and finely-ground shalevs. coarsely-ground shale, larvae exhibited statistically significant preferences for the finely-ground shales (P<0.001), coarsely-ground shales (P<0.001), and coarsely-ground shales (P<0.01), respectively. It is concluded that larvae (1) actively select shale or high-clay content sediments, (2) can differentiate among sediments with differing physical properties and (3) exhibit behavioral choices for sediment types that guide them toward shale outcrops.     

Microscale oxygen distribution in various invertebrate burrow walls

Profiles of dissolved oxygen were measured in pore waters of unburrowed sediment and the burrow walls of seven invertebrate dwellings. Burrows studied include those of Corophium volutator, Heteromastus filiformis, Arenicola marina, Saccoglossus bromophenolosus, Clymenella sp., Hemigrapsus oregonensis and Cirriformia luxuriosa all from mudflats in Willapa Bay, Washington. These animals comprise a range of burrow architectures ranging from simple, unlined burrows to more complex, mucous lined burrows. Oxygen penetrated unburrowed sediment between depths of 0.4–2.6 mm, whereas oxygen penetrated the burrow walls from 0.3 mm to 2.3 mm. Three groups of burrows are recognized based on the oxygen diffusive properties relative to the unburrowed sediment including those that: (1) slightly impeded oxygen penetration, (2) clearly inhibited oxygen penetration, and (3) enhanced oxygen penetration. Differences in the diffusive properties of the burrow wall are related to the burrow microstructure and presumably the microbial communities living within the burrow microenvironment. The results of this study suggest that burrow shape and burrow‐wall architecture may play an important role in controlling the diffusion of oxygen, and possibly of other dissolved gases (i.e. CO₂, H₂S). The results further demonstrate that simplified assumptions (i.e. that bioturbation uniformly enhances oxygen diffusion into suboxic and anoxic sediments), while requisite for numerical modelling, are not necessarily representative of field data.     

Burrow utilization by yellowedge grouper,Epinephelus flavolimbatus,in the northwestern Gulf of Mexico

Synopsis Submersible dives were made on a site in the Gulf of Mexico 160 km southeast of Galveston, Texas in September 1984. Both yellowedge, Epinephelus flavolimbatus, and snowy grouper, E. niveatus, were observed utilizing shelter around rock ridge habitats. The yellowedge grouper also sought shelter within three types of burrows cut into soft sediment. Many of these burrows were significant excavations consisting of large trenches 7–8 m long, 2–3 m wide, and 1–1.5 m deep. Burrows were found in depths from 265 to 290 m. Tilefish, Lopholatilus chamaeleonticeps, also occur at this site, usually inhabiting the characteristic vertical burrows already described for this species. In four daytime submersible transects covering a linear distance of over 13000 m, we observed a total of 66 yellowedge groupers. Twenty-five were in burrows, 39 among rocks, and two over open bottom. It is suggested that this species may have an advantage over congeners that utilize only rocky habitat for cover. It may also compete for shelter with tilefish at depths where the two species overlap.Contribution No. 696, Harbor Branch Foundation, Inc. and Contribution No. 733, The University of Texas Marine Science Institute.     

Three-Dimensional Morphological and Permeability Modelling of Diplocraterion

Three-dimensional mini-permeametry of Diplocraterion burrows from the Middle Jurassic Scarborough Formation of North Yorkshire, UK, reveals some of the relationships between ichnofossils and reservoir quality in conventional siliciclastic petroleum reservoir facies. This work focuses on the effect that the various Diplocraterion burrowing behaviours have on the permeability characteristics of the near-burrow environment. Samples were studied using serial grinding and mini-permeametry. This is the first documented attempt at combining these techniques to create an integrated understanding of the inter relationship of trace fossil morphology and reservoir quality in three dimensions. Palaeobiological insights arising from the three-dimensional modelling include evidence for resuspension feeding, deposit feeding, sediment cleaning and “collapse-cone feeding.” Higher than expected permeability values (>150 mD) were measured from the sandy shafts of Diplocraterion and adjacent areas. Zones of enhanced permeability associated with the inter-shaft area are inferred to result from sediment cleaning, and grain-size selective deposit feeding. Bioturbation by Diplocraterion is found to improve reservoir quality by sediment cleaning and creation of highly permeable vertical conduits in a reservoir facies with otherwise low permeability.     

Architectural complexity of marine crustacean burrows: unusual helical trace fossils from the Miocene of Mallorca,Spain

Gibert, J.M. de, Mas, G. & Ekdale, A.A. 2012: Architectural complexity of marine crustacean burrows: unusual helical trace fossils from the Miocene of Mallorca, Spain. Lethaia, Vol. 45, pp. 574–585. Unusual helical trace fossils occur in Tortonian shelfal calcarenites in the island of Mallorca. Their morphology may be either simple (ichnogenus Gyrolithes) or, more commonly, consist of two concentric helical burrows (ichnogenus Lapispira). They bear a very characteristic pelleted lining and are associated and probably connected to Thalassinoides and Ophiomorpha burrow systems very abundant in the same unit. These features allow the interpretation that the tracemaker was a thalassinidean shrimp. The complex and compound nature of these trace fossils is comparable to that seen in other modern and fossil crustacean burrow systems, and it reflects the behavioural plasticity of the architects. □Trace fossils, ichnology, ichnofabrics, crustacean burrows, Miocene, shallow marine, Lapispira.     

Influences of Infaunal Burrows on the Community Structure and Activity of Ammonia-Oxidizing Bacteria in Intertidal Sediments

Influences of infaunal burrows constructed by the polychaete (Tylorrhynchus heterochaetus) on O₂ concentrations and community structures and abundances of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in intertidal sediments were analyzed by the combined use of a 16S rRNA gene-based molecular approach and microelectrodes. The microelectrode measurements performed in an experimental system developed in an aquarium showed direct evidence of O₂ transport down to a depth of 350 mm of the sediment through a burrow. The 16S rRNA gene-cloning analysis revealed that the betaproteobacterial AOB communities in the sediment surface and the burrow walls were dominated by Nitrosomonas sp. strain Nm143-like sequences, and most of the clones in Nitrospira-like NOB clone libraries of the sediment surface and the burrow walls were related to the Nitrospira marina lineage. Furthermore, we investigated vertical distributions of AOB and NOB in the infaunal burrow walls and the bulk sediments by real-time quantitative PCR (Q-PCR) assay. The AOB and Nitrospira-like NOB-specific 16S rRNA gene copy numbers in the burrow walls were comparable with those in the sediment surfaces. These numbers in the burrow wall at a depth of 50 to 55 mm from the surface were, however, higher than those in the bulk sediment at the same depth. The microelectrode measurements showed higher NH₄⁺ consumption activity at the burrow wall than those at the surrounding sediment. This result was consistent with the results of microcosm experiments showing that the consumption rates of NH₄⁺ and total inorganic nitrogen increased with increasing infaunal density in the sediment. These results clearly demonstrated that the infaunal burrows stimulated O₂ transport into the sediment in which otherwise reducing conditions prevailed, resulting in development of high NH₄⁺ consumption capacity. Consequently, the infaunal burrow became an important site for NH₄⁺ consumption in the intertidal sediment.     

Reworked marine sandstone concretions: a record of high-frequency shallow burial to exhumation cycles

Concretions, with abundant calcite-dolomite cement-replacement textures originally hosted in shallow-marine sandstones, were reworked into Lower Cretaceous fluvio-deltaic conglomerates and shoreface sandstones (External Zones, Betic Cordillera). A cycle of host sand deposition, early diagenetic concretion formation and concretion reworking is documented: (1) Well-sorted shoreface sandstone deposited. (2) Spherical to ovoid, non-ferroan calcite-cemented concretions formed below flooding surfaces at shallow-burial depths during early eodiagenesis. Non-ferroan calcite cements were precipitated from the bicarbonate derived from seawater and from dissolution of marine bioclasts, as shown by isotope analyses. (3) Concretions were reworked and exposed on the seafloor in a high-energy setting as indicated by the presence of numerous bivalve borings (Entobia ichnofacies), laminated micritic microbial crusts around the concretions, and epilithobionts (oysters, barnacles and corals) on the concretion surface. Concretions also appear as erosional remnants on the floor of channels which were incised into the shoreline sandstone when sea-level fell. (4) The fluvio–deltaic channels were filled with sediment during flooding in the late lowstand of sea-level. (5) The concretions are partly dolomitized, and the presence of siderite, pyrite and barite in the outer part of the concretions precipitated before the dolomite, suggests that the latter formed during shallow burial.     

Scolicia shirahamensis isp. nov.: a triple-corded scolicia and its ichnological implications

Abstract

Peculiar meniscate burrows with three sediment cords occur in early to middle Miocene tidal-flat deposits of southwestern Japan. Two of the cords are situated at the bottom and the other is at its center. Detailed observations of the burrow structures and comparative neoichnological studies of modern spatangoid burrows in a tidal flat revealed that the former two were true drainage tubes and the latter was fecal in origin. The trace fossil was thus assigned to the ichnogenus Scolicia. Based on these findings, a new ichnospecies Scolicia shirahamensis isp. nov. has been described here. The central sediment cord is seemingly identical to the drainage tube of the ichnogenus Bichordites, another ichnogenus that has been commonly ascribed to a fossil spatangoid burrow, similar to Scolicia. Careless ichnogeneric identification of a spatangoid burrow, based only on the central sediment cord, therefore, may produce an incorrect identification.     

Strategies of burrowing in soft muddy sediments by diverse polychaetes

Muddy sediments are elastic solids through which morphologically diverse animals extend burrows by fracture. Muddy sediments inhabited by burrowing infauna vary considerably in mechanical properties, however, and at high enough porosities, muds can be fluidized. In this study, we examined burrowing behaviors and mechanisms of burrow extension for three morphologically diverse polychaete species inhabiting soft muddy sediments. Worms burrowed in gelatin, a transparent analog for muddy sediments, and in natural sediments in a novel viewing box enabling visualization of behaviors and sediment responses. Individuals of Scalibregma inflatum and Sternaspis scutata can extend burrows by fracture, but both also extended burrows by plastic deformation and by combinations of fracture and plastic deformation. Mechanical responses of sediments corresponded to different burrowing behaviors in Scalibregma; direct peristalsis was used to extend burrows by fracture or a combination of plastic deformation and fracture, whereas a retrograde expansive peristaltic wave extended burrows by plastic deformation. Burrowing speeds differed between behaviors and sediment mechanical responses, with slower burrowing associated with plastic deformation. Sternaspis exhibited less variability in behavior and burrowing speed but did extend burrows by different mechanisms consistent with observations of Scalibregma. Individuals of Ophelina acuminata did not extend burrows by fracture; rather individuals plastically deformed sediments similarly to individuals of the related Armandia brevis. Our results extend the range of natural sediments in which burrowing by fracture has been observed, but the dependence of burrow extension mechanism on species, burrowing behavior, and burrowing speed highlights the need for better understanding of mechanical responses of sediments to burrowers.     

Generation and evolution of oolitic shoal reservoirs in the Permo-Triassic carbonates,the South Pars Field,Iran

Ooid grainstone is the main reservoir rock in the Permo-Triassic Dalan and Kangan formations (Khuff equivalents) in many gas fields of the Persian Gulf and neighbouring areas. Ooids with a dominant aragonite mineralogy accumulated in a series of linear shoals and sand banks parallel to the shoreline, on the shallow parts of a vast epeiric carbonate platform. On the basis of the sequence stratigraphic analysis, these reservoir facies mainly developed during relative sea-level rises and increases in accommodation space. Integrated petrographic and geochemical studies reveal that ooid grainstone was altered through a complex diagenetic history, largely controlled by water chemistry, as a result of relative sea-level fluctuations. Two main types of ooid grainstone are the result: dolomitised grainstone or type H and oomouldic grainstone or type M. Dolomitised grainstone is commonly associated with the transgressive systems tract (TST), and developed under hypersaline conditions. In comparison, oomouldic grainstone is predominant in the early highstand systems tract (HST), which was affected by intensive meteoric diagenesis. Petrophysical study indicates that the reservoir properties of these rocks are largely a function of diagenesis. On the basis of their dominant pore types and diagenetic modifications, the ooid grainstone facies of the studied formations are grouped into five reservoir rock types. The spatial and temporal distribution of these rock types and their diagenetic evolution can be predicted within the sequence stratigraphic framework. Among these rock types, porosity has been greatly enhanced by dolomitisation and dissolution in the dolomitised grainstone (DG) and oomouldic grainstone (MG). On the other hand, it has been reduced by cementation and compaction in tightly cemented and compacted grainstones (CEG and COG rock types). The primary porosity has been relatively well preserved in grainstone with interparticle porosity (IPG rock type). The later porosity reduction during burial was also controlled by rock type. This study shows that despite the great burial depth, a significant amount of porosity is still preserved in oomouldic grainstone.     

Nitrification in Freshwater Sediments as Influenced by Insect Larvae: Quantification by Microsensors and Fluorescence in Situ Hybridization

Sediment-reworking macrofauna can stimulate nitrification by increasing the O₂ penetration into sediments or it can reduce nitrification by grazing on nitrifying bacteria. We investigated the influence of Chironomus riparius larvae (Insecta: Diptera) on the in situ activity, abundance, and distribution of NH₄⁺-oxidizing (AOB) and NO₂^–-oxidizing bacteria (NOB) in two freshwater sediments with microsensors and fluorescence in situ hybridization. In organic-poor sediment, nitrification activity was reduced by the presence of C. riparius larvae, whereas no such effect was detected in organic-rich sediment. We explain this difference with the variable larval burrowing and grazing behavior in the two sediment types: In organic-poor sediment larval activities were intense and evenly distributed across the whole sediment surface, whereas in organic-rich sediment larval activities were locally restricted to the microenvironment of animal burrows. Surprisingly, the animals did not cause any significant change of the abundance of AOB and NOB. This implies that the observed reduction of nitrification activity was not density-regulated, but rather was due to the lowered metabolic activity of the nitrifiers. Partial digestion and redeposition of particle-associated bacteria by C. riparius larvae are believed to have caused this loss of metabolic activity.This revised version was published online in November 2004 with corrections to Volume 48.     

Fine-scale environmental heterogeneities of tidal creeks affect distribution of crab burrows in a Chinese salt marsh

Tidal creeks are an important structure of salt marshes in estuarine ecosystems, providing valuable ecosystem services to wildlife in the estuary. To determine the effects of environmental heterogeneities within tidal creeks on the features of crab burrows, we divided a typical creek section into four parts (i.e., microhabitats): bottom, slope, edge and flat, investigated the distribution of crab burrows and sediment properties on creek sections in the Yangtze River estuary, and compared the burrow distribution in tidal creeks with that in non-creek areas. Our results showed that from the creek bottom to flat soil water content declined (F_3, 60 = 93.8, p < 0.001), and the variations of other sediment physical and chemical properties associated with the change of soil water content were significant among the microhabitats on the creek sections (p < 0.001 for pH, conductivity, and grain size). No crab burrows were found at the creek bottom. The burrows on the slope were smaller in size (p < 0.001 for burrow opening diameter) while the density was higher than that at the edge and on the flat (F_2, 45 = 31.2, p < 0.001). Moreover, although the correlations between burrow distribution and sediment properties varied among the microhabitats on the creek sections, crabs generally selected relatively solid sediments to build their burrows. On the slope, there was a significantly negative relationship between burrow density and soil water content (r² = 0.53, p < 0.001). At the edge, the correlation between total burrow opening area and soil water content was significantly negative (r² = 0.44, p < 0.002). The density of small crab burrows (<10 mm) was greater, but that of large burrows (>10 mm) was lower in tidal creeks than in non-creek habitats. Therefore, sediment properties showed a gradual transition from hydrophytic to terrestrial environments on the creek section, which caused significant differences of burrow distribution among the microhabitats. The creeks of tidal salt marshes could affect ecological processes and functioning through affecting crab burrows.     

Spatial and Temporal Variability in Sediment Denitrification Within an Agriculturally Influenced Reservoir

Reservoirs are intrinsically linked to the rivers that feed them, creating a river–reservoir continuum in which water and sediment inputs are a function of the surrounding watershed land use. We examined the spatial and temporal variability of sediment denitrification rates by sampling longitudinally along an agriculturally influenced river–reservoir continuum monthly for 13 months. Sediment denitrification rates ranged from 0 to 63 μg N₂O g ash free dry mass of sediments (AFDM)⁻¹ h⁻¹ or 0–2.7 μg N₂O g dry mass of sediments (DM)⁻¹ h⁻¹ at reservoir sites, vs. 0–12 μg N₂O gAFDM⁻¹ h⁻¹ or 0–0.27 μg N₂O gDM⁻¹ h⁻¹ at riverine sites. Temporally, highest denitrification activity traveled through the reservoir from upper reservoir sites to the dam, following the load of high nitrate (NO₃⁻-N) water associated with spring runoff. Annual mean sediment denitrification rates at different reservoir sites were consistently higher than at riverine sites, yet significant relationships among theses sites differed when denitrification rates were expressed per gDM vs. per gAFDM. There was a significant positive relationship between sediment denitrification rates and NO₃⁻-N concentration up to a threshold of 0.88 mg NO₃⁻ -N l⁻¹, above which it appeared NO₃⁻-N was no longer limiting. Denitrification assays were amended seasonally with NO₃⁻-N and an organic carbon source (glucose) to determine nutrient limitation of sediment denitrification. While organic carbon never limited sediment denitrification, all sites were significantly limited by NO₃⁻-N during fall and winter when ambient NO ₃⁻-N was low.