Lentisphaera araneosa gen. nov., sp. nov, a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae

Two phylogenetically distinct marine strains producing transparent exopolymers (TEP), designated HTCC2155(T) and HTCC2160, were cultivated from Oregon coast seawater by dilution to extinction in a high throughput culturing format. When cultured in low-nutrient seawater media, these strains copiously produced Alcian Blue-stainable viscous TEP. Growing cells were attached to each other by the TEP in a three dimensional network. Polymerase chain reaction employing 16S rDNA primers specific for the novel isolates indicated that they are indigenous to the water column of the Atlantic and Pacific oceans. The abundance of the isolates as determined by 16S rRNA dot blots, however, indicated that they are less than 1% of the total bacterial community. In phylogenetic analyses, the strains consistently formed a new phylum-level lineage within the domain Bacteria, together with members of the candidate phylum VadinBE97, which consists of Victivallis, the first cultured genus in the candidate phylum, and 16S rRNA gene clones from DNA extracted from marine or anaerobic terrestrial habitats. Five putative subgroups were delineated within this phylum-level lineage, including a marine group and an anaerobic group. The isolates are Gram negative, strictly aerobic, chemoheterotrophic, and facultatively oligotrophic sphere-shaped bacteria. The DNA G+C content of strain HTCC2155(T) was 48.3 mol% and the genome size was 2.9 mb. It is proposed from these observations that the strains be placed into a new genus and a new species named Lentisphaera araneosa (type strain HTCC2155(T) = ATCC BAA-859(T) = KCTC 12141(T)) gen. nov., sp. nov., the cultured marine representative of the Lentisphaerae phyl. nov., and the phylum be divided into two novel orders named the Lentisphaerales ord. nov. and the Victivallales ord. nov.     

Genome Sequences of Pelagibaca bermudensis HTCC2601T and Maritimibacter alkaliphilus HTCC2654T,the Type Strains of Two Marine Roseobacter Genera

Pelagibaca bermudensis HTCC2601^T and Maritimibacter alkaliphilus HTCC2654^T represent two marine genera in the globally significant Roseobacter clade of the Alphaproteobacteria. Here, we present the genome sequences of these organisms, isolated from the Sargasso Sea using dilution-to-extinction culturing, which offer insight into the genetic basis for the metabolic and ecological diversity of this important group.Organisms from the Roseobacter clade of the Alphaproteobacteria are numerically significant in the world''s oceans and have been found in a wide range of habitats (1, 3). Using previously described high-throughput dilution-to-extinction culturing (6, 13), the marine Roseobacter strains Pelagibaca bermudensis HTCC2601^T and Maritimibacter alkaliphilus HTCC2654^T were isolated in low-nutrient heterotrophic medium (LNHM) (4) from surface water collected at the Bermuda Atlantic Time-Series Study (BATS) site in the western Sargasso Sea (5, 9). As the type strains for two genera of this globally prolific Roseobacter group, P. bermudensis and M. alkaliphilus were selected for shotgun genome sequencing at the J. Craig Venter Institute through the Moore Foundation Microbial Genome Sequencing Project (http://www.moore.org/microgenome). Draft genomes of P. bermudensis and M. alkaliphilus, with 103 and 46 contigs, respectively, were annotated and analyzed through the Joint Genome Institute IMG/M website (http://img.jgi.doe.gov/cgi-bin/pub/main.cgi) (10).The draft genomes of P. bermudensis and M. alkaliphilus comprise 5,425,920 and 4,529,231 bases, 5,522 and 4,764 predicted open reading frames (ORFs), and 66.44% and 64.13% G+C content, respectively. The P. bermudensis genome is predicted to contain 56 tRNA genes, five 5S rRNA genes, four 16S rRNA genes, and five 23S rRNA genes, and that of M. alkaliphilus 49 tRNA genes and one each of the 5S, 16S, and 23S rRNA genes. Both genomes have putative genes for complete glycolysis and Entner-Doudoroff pathways, a complete tricarboxylic acid cycle, and predicted metabolic pathways for the oxidation of C₁ compounds. Both have predicted genes for the synthesis of most essential amino acids and some vitamins and cofactors. Each has putative genes for the utilization of fructose, sucrose, and mannose, confirmed in physiological testing of P. bermudensis (5) but not for M. alkaliphilus (9). P. bermudensis contains a predicted complete RuBisCO complex, unique to the sequenced Roseobacter species (12, 15), a complete assimilatory nitrate reduction pathway, and several type VI secretion genes. M. alkaliphilus is predicted to have complete nitrate reduction pathways to both N₂ and ammonia and most type IV secretion genes. Both are predicted to have complete sec pathways and large numbers of ABC transporters (362 in P. bermudensis and 224 in M. alkaliphilus), similar to other Roseobacter strains (15).M. alkaliphilus was named because of its alkaline growth optimum at pH 10. Na⁺/H⁺ antiporters have been shown to be involved in conferring alkaliphilic phenotypes for a variety of organisms by increasing internal cellular H⁺ concentrations in alkaline conditions where Na⁺ is present (2, 7, 8, 14, 16, 17). As expected, the genome of M. alkaliphilus contains two putative Na⁺/H⁺ antiporters, one homologous to nhaP, important for alkaliphily in several strains (2, 16, 17), and another located adjacent to predicted ABC transporter genes for capsular polysaccharide export.     

Genome Sequences of Oceanicola granulosus HTCC2516T and Oceanicola batsensis HTCC2597T

Genome sequences from the prolific Roseobacter clade in the Alphaproteobacteria are beginning to reveal the genetic basis for the diverse lifestyles of these organisms. Here we present the genome sequences of Oceanicola granulosus HTCC2516^T and Oceanicola batsensis HTCC2597^T, two marine Roseobacter species isolated from the Sargasso Sea using dilution-to-extinction culturing, whose genomes encode for significant differences in metabolic potential.Members of the Roseobacter clade are ecologically and physiologically diverse, occupying a wide variety of lifestyles (1, 2, 7). Oceanicola granulosus HTCC2516^T and Oceanicola batsensis HTCC2597^T were isolated by dilution-to-extinction culturing in low-nutrient heterotrophic media (LNHM) (4) from water collected at the Bermuda Atlantic Time-Series Study (BATS) station in the Sargasso Sea (3, 5). The genomes were shotgun sequenced by the J. Craig Venter Institute as part of the Moore Foundation Microbial Genome Sequencing Project (http://www.moore.org/microgenome). Draft genomes of O. granulosus and O. batsensis containing 85 and 23 contigs, respectively, were annotated analyzed through the Joint Genome Institute IMG/M website (http://img.jgi.doe.gov/cgi-bin/pub/main.cgi) (6). The draft genomes of O. granulosus and O. batsensis comprised 4,039,111 and 4,437,668 bases, 3,855 and 4,261 predicted open reading frames (ORFs), and 70.41% and 66.10% G+C contents, respectively. The O. granulosus genome is predicted to contain 55 tRNA genes, two 5S rRNA genes, four 16S rRNA genes, and two 23S rRNA genes; that of O. batsensis is predicted to contain 45 tRNA genes, one each of the 5S and 16S rRNA genes, and two 23S rRNA genes.The sequencing of these and other Roseobacter genomes has directly affected the Oceanicola phylogeny. Recent phylogenetic studies of the Alphaproteobacteria and the Roseobacter clade specifically have established that the Oceanicola genus is not monophyletic (1, 7, 11). Not surprisingly, the two genomes reveal different potential capabilities for these two organisms. O. granulosus is predicted to possess several genes necessary for the Calvin cycle, including the large (but not the small) RuBisCo (ribulose-1,5-bisphosphate carboxylase) subunit. Both strains tested negative for fructose utilization (3) but contain putative genes necessary for this metabolism. Consistent with physiological tests showing glucose utilization, O. granulosus is predicted to have complete glycolysis, pentose-phosphate, and Entner-Doudoroff (ED) pathways. O. batsensis has only a putative ED pathway, but did not utilize glucose (3). Both strains were characterized as nonmotile (3), and O. batsensis has no che gene homologs, but O. granulosas is predicted to have most flagellar and che gene homologs. O. granulosus has putative genes for an aa₃-type cytochrome c oxidase, and O. batsensis is predicted to have both aa₃- and cbb₃-type cytochrome c oxidases, the latter potentially conferring an adaptive advantage over a greater range of oxygen concentrations (8, 9).Both organisms are predicted to have most Sec pathway genes. O. batsensis contains several putative type IV secretion genes and 6 putative TonB receptors. Each has many predicted ABC transporter genes: 239 in O. granulosus and 193 in O. batsenesis. Both organisms were observed to form polyhydroxybutyrate (PHB) in culture (3), and both organisms contain putative genes necessary for PHB synthesis (see reference 10 and references therein), including predicted copies of PHB or polyhydroxyalkanoate (PHA) polymerases and several acetyl coenzyme A (acetyl-CoA) acetyltransferases, and O. batsensis has a predicted acetoacetyl-CoA reductase.     

Genome Sequence of the Oligotrophic Marine Gammaproteobacterium HTCC2143, Isolated from the Oregon Coast

Strain HTCC2143 was isolated from Oregon Coast surface waters using dilution-to-extinction culturing. Here we present the genome of strain HTCC2143 from the BD1-7 clade of the oligotrophic marine Gammaproteobacteria group. The genome of HTCC2143 contains genes for carotenoid biosynthesis and proteorhodopsin and for proteins that have potential biotechnological significance: epoxide hydrolases, Baeyer-Villiger monooxygenases, and polyketide synthases.Strain HTCC2143 was sampled and isolated from surface waters (depth, 10 m) off the Coastal Pacific Ocean, Newport, OR (44°36′0N, 124°6′0W). In the course of dilution-to-extinction culture studies on coastal microbial communities, strain HTCC2143 was isolated in a pristine seawater-based medium (2). Phylogenetic analysis of 16S rRNA gene sequences placed strain HTCC2143 in the BD1-7 clade of the oligotrophic marine Gammaproteobacteria (OMG) group (2) and indicated that it is related to Dasania marina, isolated from Arctic marine sediment (3, 8). The HTCC2143 16S rRNA gene sequence is 95.3% similar to that of D. marina ({type:entrez-nucleotide,attrs:{text:AY771747,term_id:157384056,term_text:AY771747}}AY771747) and is 96.6% similar to that of environmental gene clone 20m-45 ({type:entrez-nucleotide,attrs:{text:GU061297,term_id:262072220,term_text:GU061297}}GU061297), taken from intertidal beach seawater of the Yellow Sea, South Korea. Other closer relatives of HTCC2143 included uncultured gammaproteobacterial clones from seafloor lava (clone P0X3b5B06 from Hawaii South Point X3, {type:entrez-nucleotide,attrs:{text:EU491383,term_id:169640639,term_text:EU491383}}EU491383; 96.3%) (9), deep-sea sediment (Ucp1554 from the South Atlantic Ocean, Cape Basin, {type:entrez-nucleotide,attrs:{text:AM997645,term_id:283475537,term_text:AM997645}}AM997645; 95.9%) (10), Yellow Sea sediment (95.8%; D8S-33, {type:entrez-nucleotide,attrs:{text:EU652559,term_id:186462116,term_text:EU652559}}EU652559), and Arctic sediment (from Kings Bay, Svalbard, Norway; clone SS1_B_07_55, {type:entrez-nucleotide,attrs:{text:EU050825,term_id:156453922,term_text:EU050825}}EU050825; 95.7%).Genomic DNA was prepared at Oregon State University and sequenced by the J. Craig Venter Institute. The finished contigs were automatically annotated with a system based on the program GenDB (5) and manually annotated as described in previous reports (7, 12). The annotation is available at http://bioinfo.cgrb.oregonstate.edu/microbes/. The draft genome of strain HTCC2143 comprises 3,925,629 bases and 3,662 predicted coding sequences with a G+C content of 47.0%. The genome of HTCC2143 was predicted to contain 40 tRNAs, 1 16S rRNA, 2 5S rRNAs, and 2 23S rRNA genes. Four genes for selenocysteine metabolism were found, including a selenophosphate-dependent tRNA 2-selenouridine synthase and an l-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1).Strain HTCC2143 had genes for a complete tricarboxylic acid cycle, glycolysis, a pentose phosphate pathway, and an Entner-Doudoroff pathway. Genes were present for a high-affinity phosphate transporter and a pho regulon for sensing of environmental inorganic phosphate availability, as well as genes from the NUDIX (nucleoside diphosphate linked to some other moiety X) hydrolase domain family (1) that reflects the metabolic complexity of prokaryotes (4). Genes for ammonium transporters, nitrate reductase, and sulfate reductase were also present in the HTCC2143 genome.Carotenoid and proteorhodopsin genes were also found in the genome, as well as genes for polyketide synthase modules and related proteins. Carotenoid and proteorhodopsin genes were reported previously from another member of the OMG group, strain HTCC2207, a SAR92 clade isolate (11). HTCC2143 also encoded two epoxide hydrolases, two cyclohexanone monooxygenases (CHMOs) and a cyclododecanone monooxygenase (CDMO). CDMOs and CHMOs are members of the Baeyer-Villiger monooxygenase (BVMO) family. BVMOs are “green” alternatives to the chemically mediated Baeyer-Villiger reactions that allow the conversion of ketones into esters or of cyclic ketones into lactones (6).This genome provides further evidence that dilution-to-extinction culturing methods that make use of low-nutrient media that are similar to the conditions of the natural environment can result in the isolation of novel, environmentally significant organisms with potential biotechnological value (13).     

Complete Genome Sequence of an Uncultured Bacterium of the Candidate Phylum Bipolaricaulota

Microbiology - Bacteria of the candidate phylum Bipolaricaulota, previously known as OP1 and Acetothermia, have been identified in various terrestrial and marine ecosystems as a result of molecular...     

Complete Genome Sequence of Croceibacter atlanticus HTCC2559T

Here we announce the complete genome sequence of Croceibacter atlanticus HTCC2559^T, which was isolated by high-throughput dilution-to-extinction culturing from the Bermuda Atlantic Time Series station in the Western Sargasso Sea. Strain HTCC2559^T contained genes for carotenoid biosynthesis, flavonoid biosynthesis, and several macromolecule-degrading enzymes. The genome confirmed physiological observations of cultivated Croceibacter atlanticus strain HTCC2559^T, which identified it as an obligate chemoheterotroph.The phylum Bacteroidetes comprises 6 to ∼30% of total bacterial communities in the ocean by fluorescence in situ hybridization (8-10). Most marine Bacteroidetes are in the family Flavobacteriaceae, most of which are aerobic respiratory heterotrophs that form a well-defined clade by 16S rRNA phylogenetic analyses (4). The members of this family are well known for degrading macromolecules, including chitin, DNA, cellulose, starch, and pectin (17), suggesting their environmental roles as detritus decomposers in the ocean (6). Marine Polaribacter and Dokdonia species in the Flavobacteriaceae have also shown to have photoheterotrophic metabolism mediated by proteorhodopsins (11, 12).Several strains of the family Flavobacteriaceae were isolated from the Sargasso Sea and Oregon coast, using high-throughput culturing approaches (7). Croceibacter atlanticus HTCC2559^T was cultivated from seawater collected at a depth of 250 m from the Sargasso Sea and was identified as a new genus in the family Flavobacteriaceae based on its 16S rRNA gene sequence similarities (6). Strain HTCC2559^T met the minimal standards for genera of the family Flavobacteriaceae (3) on the basis of phenotypic characteristics (6).Here we report the complete genome sequence of Croceibacter atlanticus HTCC2559^T. The genome sequencing was initiated by the J. Craig Venter Institute as a part of the Moore Foundation Microbial Genome Sequencing Project and completed in the current announcement. Gaps among contigs were closed by Genotech Co., Ltd. (Daejeon, Korea), using direct sequencing of combinatorial PCR products (16). The HTCC2559^T genome was analyzed with a genome annotation system based on GenDB (14) at Oregon State University and with the NCBI Prokaryotic Genomes Automatic Annotation Pipeline (15, 16).The HTCC2559^T genome is 2,952,962 bp long, with 33.9 mol% G+C content, and there was no evidence of plasmids. The number of protein-coding genes was 2,715; there were two copies of the 16S-23S-5S rRNA operon and 36 tRNA genes. The HTCC2559^T genome contained genes for a complete tricarboxylic acid cycle, glycolysis, and a pentose phosphate pathway. The genome also contained sets of genes for metabolic enzymes involved in carotenoid biosynthesis and also a serine/glycine hydroxymethyltransferase, which is often associated with the assimilatory serine cycle (13). The potential for HTCC2559^T to use bacterial type III polyketide synthase (PKS) needs to be confirmed because this organism had a naringenin-chalcone synthase (CHS) or chalcone synthase (EC 2.3.1.74), a key enzyme in flavonoid biosynthesis. CHS initiates the addition of three molecules of malonyl coenzyme A (malonyl-CoA) to a starter CoA ester (e.g., 4-coumaroyl-CoA) (1) and takes part in a few bacterial type III polyketide synthase systems (1, 2, 5, 18).The complete genome sequence confirmed that strain HTCC2559^T is an obligate chemoheterotroph because no genes for phototrophy were found. As expected from physiological characteristics (6), the HTCC2559^T genome contained a set of genes coding for enzymes required to degrade high-molecular-weight compounds, including peptidases, metallo-/serine proteases, pectinase, alginate lyases, and α-amylase.     

Genome Sequence of the Thermophile Bacillus coagulans Hammer,the Type Strain of the Species

Here we announce a 3.0-Mb assembly of the Bacillus coagulans Hammer strain, which is the type strain of the species within the genus Bacillus. Genomic analyses based on the sequence may provide insights into the phylogeny of the species and help to elucidate characteristics of the poorly studied strains of Bacillus coagulans.     

Complete Genome Sequence of Methanomassiliicoccus luminyensis, the Largest Genome of a Human-Associated Archaea Species

The present study describes the complete and annotated genome sequence of Methanomassiliicoccus luminyensis strain B10 (DSM 24529(T), CSUR P135), which was isolated from human feces. The 2.6-Mb genome represents the largest genome of a methanogenic euryarchaeon isolated from humans. The genome data of M. luminyensis reveal unique features and horizontal gene transfer events, which might have occurred during its adaptation and/or evolution in the human ecosystem.     

Genome Sequence of Fulvimarina pelagi HTCC2506T,a Mn(II)-Oxidizing Alphaproteobacterium Possessing an Aerobic Anoxygenic Photosynthetic Gene Cluster and Xanthorhodopsin

Fulvimarina pelagi is a Mn(II)-oxidizing marine heterotrophic bacterium in the order Rhizobiales. Here we announce the draft genome sequence of F. pelagi HTCC2506^T, which was isolated from the Sargasso Sea by using dilution-to-extinction culturing. The genome sequence contained a xanthorhodopsin gene as well as a photosynthetic gene cluster, which suggests the coexistence of two different phototrophic mechanisms in a single microorganism.Besides being mediated by the well-known process of aerobic oxygenic photosynthesis, utilization of light energy in the marine carbon and nutrient cycling processes is usually mediated by aerobic anoxygenic phototrophic bacteria (AAPB) or prokaryotes containing microbial rhodopsin family proteins (18). AAPB comprise about 10% of total microbial cells in the euphotic zone of diverse marine regimes, with alpha-, beta-, and gammaproteobacteria as major constituents (8, 10). Rhodopsin family proteins, including bacteriorhodopsin, proteorhodopsin, and xanthorhodopsin (XR), have been shown to exist in 7 to 70% of marine prokaryotes and contribute photoheterotrophy in diverse phylogenetic groups such as Flavobacteria, Proteobacteria, and Archaea (15, 19). However, no single microorganism has been reported to contain the genes for both aerobic anoxygenic phototrophy (AAnP) and rhodopsin family proteins.F. pelagi HTCC2506^T was cultivated through a dilution-to-extinction approach (3) from the western Sargasso Sea and identified as a novel genus and species in the order Rhizobiales of the Alphaproteobacteria by polyphasic taxonomy (2). Later, three F. pelagi strains, including HTCC2506^T, were shown to have Mn(II)-oxidizing activity (1). The draft genome sequence of HTCC2506^T was determined by shotgun sequencing at the J. Craig Venter Institute as part of the Moore Foundation Microbial Genome Sequencing Project and analyzed by the GenDB annotation program (17) at the Center for Genome Research and Biocomputing at Oregon State University and the Joint Genome Institute IMG system (http://img.jgi.doe.gov) (14).The draft genome was 3,802,689 bp in length, distributed in 20 contigs with 61.2% G+C content, and contained 3,754 protein-coding genes, three copies of 16S-23S-5S rRNA genes, and 54 tRNA genes. Remarkably, HTCC2506^T possessed XR and a complete gene set for AAnP together. A gene cluster encoding the AAnP apparatus was composed of bchIDO-crtCDF-bchCXYZ-pufBALMC-puhE-acsF-puhCBA-lhaA-bchMLHBNF-aerR-ppsR-ubiA-pucC-bchP-hemT. Mu-like prophage sequences were located closely adjacent to the AAnP gene cluster, which might imply the possibility of the lateral gene transfer of the AAnP gene cluster. The XR gene was followed by blh, a gene involved in retinal biosynthesis (16). To our knowledge, this is the first report of a microbe possessing both an AAnP apparatus and a rhodopsin family protein, although the two gene sets in HTCC2506^T had been separately reported to be present (5, 13). The HTCC2506^T genome also encoded a bacteriophytochrome (6, 7), a light-regulated signal transduction histidine kinase. Overall, the existence of a diverse repertoire of genes for sensing or harvesting of light energy implicates the importance of phototrophic metabolism for HTCC2506^T.In addition to genes for phototrophy, the genome contained several genes with diverse metabolic potential. A lithotrophic mode of energy acquisition was predicted from the presence of the form II coxSLM genes, which encode aerobic-type carbon monoxide dehydrogenase (9). As expected from the Mn(II)-oxidizing activity of HTCC2506^T, the genome also encoded a multicopper oxidase (MCO) enzyme, suggesting a potential lithotrophy. In terms of carbon assimilation, genes encoding RuBisCO and phosphoribulokinase of the Calvin-Benson-Bassham cycle (11) were predicted, suggesting the possibility of autotrophic CO₂ fixation in HTCC2506^T. Serine transhydroxymethylase for formaldehyde assimilation (12) was predicted. A gene encoding dimethylsulfoniopropionate (DMSP) lyase, which conveys the production of dimethylsulfide from DMSP (4), was also found in the genome.Although HTCC2506^T was originally isolated as a chemoheterotroph (2), the genome sequence clearly shows the phototrophic potential of this bacterium. This finding, combined with the prediction of many genes related to lithotrophy, carbon fixation, C₁ compound assimilation, and DMSP lysis, suggests that F. pelagi HTCC2506^T may use a wide range of potential metabolic functions to survive in the marine euphotic environment.     

Genome sequence of the marine alphaproteobacterium HTCC2150, assigned to the Roseobacter clade

Here we announce the genome sequence of a marine bacterium, HTCC2150, that was isolated off the Oregon coast using dilution-to-extinction culturing and that is affiliated with the Roseobacter clade. The 16S rRNA phylogeny showed that the strain was closely related to members of the RCA clade. The genome sequence suggests that strain HTCC2150 is an organoheterotroph carrying diverse metabolic potential, including a close relationship with phytoplankton.     

Genome Sequence of Brucella melitensis S66, an Isolate of Sequence Type 8, Prevalent in China

Brucella melitensis is the most-represented Brucella species causing human brucellosis in China. Here we report the complete genome sequence of B. melitensis strain S66, a representative strain of sequence type 8 (ST8), which is prevalent in China, making it possible to compare the genome sequences of isolates from different countries.     

Complete Genome Sequence of Canine Papillomavirus Type 10

Papillomaviruses are epitheliotropic, nonenveloped, circular, double-stranded DNA viruses within the family Papillomaviridae that are associated with benign and malignant tumors in humans and animals. We report the complete genome sequence of canine papillomavirus type 10 identified from a pigmented plaque located on the head of a mixed-breed bloodhound.     

Complete Genome Sequence of Equine Herpesvirus Type 9

Equine herpesvirus type 9 (EHV-9), which we isolated from a case of epizootic encephalitis in a herd of Thomson''s gazelles (Gazella thomsoni) in 1993, has been known to cause fatal encephalitis in Thomson''s gazelle, giraffe, and polar bear in natural infections. Our previous report indicated that EHV-9 was similar to the equine pathogen equine herpesvirus type 1 (EHV-1), which mainly causes abortion, respiratory infection, and equine herpesvirus myeloencephalopathy. We determined the genome sequence of EHV-9. The genome has a length of 148,371 bp and all 80 of the open reading frames (ORFs) found in the genome of EHV-1. The nucleotide sequences of the ORFs in EHV-9 were 86 to 95% identical to those in EHV-1. The whole genome sequence should help to reveal the neuropathogenicity of EHV-9.     

Genome sequence of strain HTCC2083, a novel member of the marine clade Roseobacter

Strain HTCC2083 was isolated from Oregon seawater using dilution-to-extinction culturing and represents a novel member of the Roseobacter clade. The draft genome sequence of HTCC2083 is presented here. The genome is predicted to contain genes for aerobic anoxygenic phototrophy, sulfite-oxidizing chemolithotrophy, anapleurotic CO(2) fixation, carbon monoxide oxidation, and dimethylsulfoniopropionate (DMSP) utilization.     

Draft Genome Sequence of Actinomyces massiliensis Strain 4401292T

A draft genome sequence of Actinomyces massiliensis, an anaerobic bacterium isolated from a patient's blood culture, is described here. CRISPR-associated proteins, insertion sequences, and toxin-antitoxin loci were found on the genome.     

Draft Genome Sequence of Lactobacillus rossiae DSM 15814T

The draft genome sequence of Lactobacillus rossiae DSM 15814(T) (CS1, ATCC BAA-88) was determined by a whole-genome shotgun approach. Reads were assembled to a 2.9-Mb draft version. RAST genome annotation evidenced 2,723 predicted coding sequences. Many carbohydrate, amino acid, and amino acid derivative subsystem features were found.