Comparison of dimethoate and methidathion tolerance in four strains ofPhytoseiulus persimilis (Athias-Henriot) (Acarina: Phytoseiidae) in Australia

The toxicity of dimethoate 30EC and methidathion 40EC was tested against four strains ofPhytoseiulus persimilis obtained from commercial greenhouse rose crops. A single strain ofP. persimilis demonstrated tolerance to the field rate of 1.0 ml/l dimethoate used to controlHeliothrips haemorrhoidalis as indicated by the LC₅₀ of 1.5 ml/l ai and a significantly different response at the LC₉₀ level (P<0.05) to the three other strains tested. None of the strains was different in level of response to methidathion (P>0.05) and were unable to tolerate the recommended field rate of methidathion. Selection to dimethoate by routine field spraying has produced improved tolerance inP. persimilis. The dimethoate-tolerant strain was not different in developmental period and mean number of eggs produced per female when compared with the Sydney strain (P>0.05).     

杀扑磷抗性获得对奥氏钝绥螨发育历期与繁殖能力的影响

为探索杀扑磷抗性获得对奥氏钝绥螨Amblyseius womersleyi Schicha发育历期与繁殖能力的影响,研究了在25℃与30℃温度下,奥氏钝绥螨的杀扑磷抗性系与敏感系的发育历期与繁殖能力。结果表明：奥氏钝绥螨的杀扑磷抗性系与敏感系的交配产卵行为习性没有差异。在25℃与30℃温度下,奥氏钝绥螨的各发育历期、产卵期、产卵高峰期,每雌日产卵量与总产卵量,卵孵化率及雌雄成螨性比等,在杀扑磷抗性系与敏感性系之间无明显差异。结果说明杀扑磷抗性获得对奥氏钝绥螨的发育历期与繁殖能力没有明显影响。     

Conditioned olfactory responses of a predatory mite, Neoseiulus womersleyi, to volatiles from prey-infested plants

To clarify the prey‐finding behavior of the predatory mite Neoseiulus womersleyi (Schicha) (Acari: Phytoseiidae), we studied its olfactory responses to volatiles from the prey‐infested plant on which the mites had been collected. We used a local N. womersleyi population called Kanaya collected from tea (Camellia sinensis L.) (Theaceae) plants infested by Tetranychus kanzawai Kishida (Acari: Tetranychidae) in Kanaya City, Japan. Neoseiulus womersleyi (Kanaya population) were more attracted to volatiles from tea plants infested with five female T. kanzawai per leaf for 7 days than to intact tea leaves in a Y‐tube olfactometer. Tetranychus kanzawai‐induced tea leaf volatiles were identified as (E)‐β‐ocimene, (E)‐4,8‐dimethyl‐1,3,7‐nonatriene, and (E,E)‐α‐farnesene. As olfactory responses are known to differ among local populations of N. womersleyi, we compared the responses of the Kanaya population with those of a Kikugawa population collected from tea plants infested by T. kanzawai in Kikugawa City. To test the influence of previous predation experience, we reared the two populations on tea plants infested by T. kanzawai or on kidney bean plants (Phaseolus vulgaris) infested by Tetranychus urticae Koch. The Kanaya population was more attracted to the volatiles from infested plants on which they had been reared. Because the Kanaya population was not attracted to the plant volatiles they had not previously experienced, the positive response to previously experienced volatiles might be the result of learning. By contrast, the Kikugawa population showed no preference for previously experienced volatiles from infested plants. The implications of this flexibility in foraging behavior are discussed.     

Baseline susceptibility of persea mite (Acari: Tetranychidae) to abamectin and milbemectin in avocado groves in Southern California

Persea mite, Oligonychus perseae Tuttle, Baker, and Abatiello, susceptibility to abamectin and milbemectin was evaluated in 2003 to determine baseline susceptibility levels in avocado groves in San Diego and Ventura Counties (California, USA) where more than 70% of the state’s avocado production is concentrated. Milbemectin has yet to be used in avocado production in California and abamectin has been available for use since 1999. Baseline susceptibility ratios (in relation to the most susceptible population) of five persea mite field strains to milbemectin varied 2.1- to 2.8-fold at the LC₅₀ and LC₉₀, respectively. The susceptibility of seven field strains to abamectin varied slightly more (2.1- to 3.5-fold) with one strain subjected to seven sprays over the past 4 years showing slight but significant separation of LC₅₀ and LC₉₀’s from the most susceptible strain, which is suggestive of the early stages of resistance to this product. Based on these data, baseline susceptibility levels are proposed that might be used to monitor for future persea mite resistance to these chemicals as their use in California avocado production continues.     

Sublethal effects of fenpyroximate and pyridaben on two predatory mite species, <Emphasis Type="Italic">Neoseiulus womersleyi</Emphasis> and <Emphasis Type="Italic">Phytoseiulus persimilis</Emphasis> (Acari,Phytoseiidae)

Laboratory bioassays were conducted to evaluate the sublethal effects of fenpyroximate and pyridaben on life-table parameters of two predatory mites species, Neoseiulus (= Amblyseius) womersleyi and Phytoseiulus persimilis. In these assays, young adult females were treated with three sublethal concentrations of each acaricide. The life-table parameters were calculated at each acaricide concentration, and compared using bootstrap procedures. For each acaricide, the LC₅₀ estimates for both species were similar, yet the two species exhibited completely different susceptibility when the population growth rate was used as the endpoint. Exposure to both acaricides reduced the net reproduction rate (R _o ) in a concentration-dependent manner and their EC₅₀s were equivalent to less than LC₇. Two different scales of population-level endpoints were estimated to compare the total effect between the species and treatments: the first endpoint values were based on the net reproductive rate (fecundity λ) and the second endpoint values incorporated the mean egg hatchability into the net reproductive rate (vitality λ). The fecundity λ decreased in a concentration-dependent manner for both acaricide treatments, but the vitality λ decreased abruptly after treatment of N. womersleyi with pyridaben. The change in the patterns of λ revealed that the acaricide effects at the population level strongly depended on the life-history characteristics of the predatory mite species and the chemical mode of action. When the total effects of the two acaricides on N. womersleyi and P. persimilis were considered, fenpyroximate was found to be the most compatible acaricide for the augmentative release of N. womersleyi after treatment.     

丁氟螨酯对二斑叶螨生长发育的影响

[目的]丁氟螨酯是一种新型酰基乙腈类非内吸性杀螨剂,对害螨的各个螨态都有很高活性,具有较高的应用价值.本文评价了丁氟螨酯对二斑叶螨生长发育的影响,以期为合理用药和二斑叶螨的综合防治提供理论依据.[方法]采用浸叶法测定丁氟螨酯对二斑叶螨成螨与卵的致死中浓度、雌成螨产卵量、各螨态存活率以及各发育历期的影响.[结果]经丁氟螨...     

Effects of the ecdysteroid agonists RH 5849 an RH 5992, alone and in combination with a juvenile hormone analogue, pyriproxyfen, on larvae ofSpodoptera exigua

Biological activity assays with RH 5849 and RH 5992 indicated that both compounds affected growth and development of last-instar larvae ofSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) in a dose-dependent manner. Within the first 24 h after treatment by continuously offering leaves dipped in a water solution of ≥50 mg/l RH 5849 and ≥0.5 mg/l RH 5992, symptoms of a prematurely induced larval moult and head capsule apolysis were visible. Intoxicated larvae died shortly afterwards, showing signs of unsuccessful ecdysis. LC₅₀-values of RH 5849 and RH 5992 for fifth-instarS. exigua larvae were 110 and 2.5 mg/l, respectively. Pyriproxyfen alone affected the larval stage and disturbed normal metamorphosis. One supernumerary larval instar occurred occasionally. LC₅₀-value for pyriproxyfen was 1.7 mg/l. Larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, continued to grow until they attained a size and weight about 2–3 times that of the controls. This growth was accompanied by at least one and sometimes two supernumerary moults. Concerning thein vivo imaginal wing disc growth and development, only in larvae treated with 10 and 50 mg/l RH 5849 or 0.5 mg/l RH 5992, tracheole migration was observed earlier than in the controls. When applying 300 mg/l RH 5849 or 3–7 mg/l RH 5992, the discs remained small and no signs of tracheole migration were observed. In larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, tracheole migration was not prematurely induced and a pupal cuticle was produced in the discs of larvae, undergoing a supernumerary moult. No clear signs of evagination were observed.     

Effects of temperature on acaricidal and insecticidal activities of the benzoylureas flucycloxuron and diflubenzuron

Effects of temperature on the activity of flucycloxuron on larval stages of Panonychus ulmi (Koch), based on LC₅₀ values, were highly significant (P < 0.001) with temperature coefficients of-1.7 in both the ranges of 15° to 25°C and 20° to 30°C. The slopes of probit regression lines at 15° and 20°C were significantly steeper than those at 25° and 30°C. As a consequence the temperature coefficients based on LC₉₀ values were-4.4 and-2.2, for the 2 temperature ranges. The ovicidal activity of flucycloxuron on P. ulmi was low and was only statistically detectable at 20°C (LC₉₀ of 84 mg a.i./l). In studies with larvae of Aedes aegypti (Linnaeus), Leptinotarsa decemlineata (Say), Plutella xylostella (Linnaeus), Spodeptera exigua (Hübner) and Spodoptera littoralis (Boisduval) probit regression lines were parallel over temperature. The activity of flucycloxuron on these five insect species was not affected by temperature. Based on LC₅₀ values, diflubenzuron showed positive temperature coefficients on P. xylostella of + 2.1 at 15° to 25°C and + 2.5 at 20° to 30°C. For S. littoralis the temperature coefficient was positive (+ 2.4) at 15° to 25°C but negative (-1.9) at the 20° to 30°C range. Temperature coefficients of diflubenzuron were neutral for A. aegypti, L. decemlineata and S. exigua. In the design and analysis of these studies special allowance was made for date effects and variation in natural mortality over temperature.     

Insecticidal and feeding deterrent activities of essential oils in the cabbage looper,Trichoplusia ni (Lepidoptera: Noctuidae)

Ten essential oils were tested against the cabbage looper, Trichoplusia ni larvae for contact, residual and fumigant toxicities and feeding deterrent effects. Against third instar T. ni, Syzygium aromaticum (LD₅₀ = 47.8 μg/larva), Thymus vulgaris (LD₅₀ = 52.0 μg/larva) (the two positive controls) and Cinnamomum glanduliferum (LD₅₀ = 76.0 μg/larva) were the most toxic via topical application. Litsea pungens (LD₅₀ = 87.1 μg/larva), Ilex purpurea (LD₅₀ = 94.0 μg/larva), Cinnamomum cassia (LD₅₀ = 101.5 μg/larva) and Litsea cubeba (LD₅₀ = 112.4 μg/larva) oils were equitoxic. Thymus vulgaris (LC₅₀ = 4.8 mg/ml) and S. aromaticum (LC₅₀ = 6.0 mg/ml) oils were the most toxic in residual bioassays. Cymbopogon citratus (LC₅₀ = 7.7 mg/ml) and C. cassia (LC₅₀ = 8.5 mg/ml) oils were equitoxic followed by Cymbopogon nardus (LC₅₀ = 10.1 mg/ml) in this bioassay. The remaining five oils showed little or no residual effects. In a fumigation bioassay, L. cubeba (LC₅₀ = 16.5 μl/l) and I. purpurea (LC₅₀ = 22.2 μl/l) oils were the most toxic. Cinnamomum glanduliferum (LC₅₀ = 29.7 μl/l) and Sabina vulgaris (LC₅₀ = 31.2 μl/l) oils were equitoxic. Interestingly, S. aromaticum did not exhibit any fumigant toxicity. Cymbopogon citratus, C. nardus and C. cassia strongly deterred feeding by third instar T. ni (DC₅₀s = 26.9, 33.8 and 39.6 μg/cm², respectively) in a leaf disc choice bioassay. The different responses of T. ni larvae to the oils in different bioassays suggest that these essential oils exhibit different modes of action. Based on their comparable efficacy with essential oils already used as active ingredients in many commercial insecticides (i.e. clove oil and thyme oil), some of these essential oils may have potential as botanical insecticides against T. ni.     

Baseline susceptibility of Helicoverpa armigera,Plutella xylostella,and Spodoptera frugiperda to the meta-diamide insecticide broflanilide

Broflanilide is a novel meta-diamide insecticide that acts as a γ-aminobutyric acid-gated chloride channel allosteric modulator. With its unique mode of action, broflanilide has no known cross-resistance with existing insecticides and is expected to be an effective tool for the management of insecticide resistance. Establishing the baseline susceptibility to this insecticide is an essential step for developing and implementing effective resistance management strategies. Here we evaluated the baseline susceptibility to broflanilide for 3 cosmopolitan lepidopteran pest species, Helicoverpa armigera, Plutella xylostella, and Spodoptera frugiperda. Broflanilide exhibited high activity against populations sampled in the major distribution range of these pests in China, with median lethal concentrations (LC₅₀) ranging between 0.209 and 0.684, 0.076 and 0.336, and 0.075 and 0.219 mg/L for H. armigera, P. xylostella, and S. frugiperda, respectively. Among-population variability in susceptibility to broflanilide was moderate for H. armigera (3.3-fold), P. xylostella (4.4-fold), and S. frugiperda (2.9-fold). The recommended diagnostic concentrations for H. armigera, P. xylostella, and S. frugiperda were 8, 4, and 2 mg/L, respectively. Little or no cross-resistance to broflanilide was detected in 3 diamide-resistant strains of P. xylostella and 1 spinosyns-resistant strain of S. frugiperda. Our results provide critical information for the development of effective resistance management programs to sustain efficacy of broflanilide against these key lepidopteran pests.     

Hymenopteran specificity of Bacillus thuringiensis strain PS86Q3

The biological activity of Bacillus thuringiensis (Bt) strain PS86Q3 against five Hymenopteran species was determined by means of bioassays adapted to each species. Four species of sawfly that are important pests of conifers (Diprion pini, Gilpinia hercyniae and Pristiphora abietina) or ornamental plants (Arge rosae), as well as the non-target honeybee, Apis mellifera, were studied. Two out of the four sawfly species tested were found to be sensitive to PS86Q3 crystals or spore/crystal suspensions. A sporulated culture of this strain was moderately active on D. pini, and a complete bioassay with solubilized crystals was performed to estimate the LC₅₀ of 4.9 mg/ml. Pristiphora abietina was also found to be sensitive to PS86Q3, with an LC₅₀ of 1.6 mg/ml. By contrast, at the concentrations tested, PS86Q3 did not prove active on the remaining sawflies, G. hercyniae and A. rosae. The strain was administered orally to check its effects on honeybees which were fed sucrose solutions supplemented with a PS86Q3 sporulated suspension, in a field assay using commercial beehives. No significant differences in larval mortality (as deduced by comparing the number of larvae, pupae and empty cells) were found between the Bt and control treatments. On the basis of the results presented here, the suitability of PS86Q3 for the control of Hymenopteran pests, particularly sawflies, in terms of both potency and environmental safety, is discussed.     

STABILITY OF ORGANOPHOSPHATE RESISTANCE IN CULEX PIPIENS PALLENS COQUILLETT UNDER RELAXATION OF INSECITICDE SELECTION*

Abstract The stability of resistance in the organophosphate resistant strain of Culex pipiens pallens with amplification of single gene coding for esterase B₂ was determined under relaxation of insecticide selection. Insecticide resistance and amplification of esterase were both lost when strains were not homozygous for the presence of amplified gene, probably due to biological disadvantage of the esterase gene. LC₅₀ and LC₉₅ of chlorpyrifos for this strain were 0. 2099 mg/L and 0.9036 mg/L in the F₀ generation respectively, but 0. 0207 mg/L and 2. 8027 mg/L respectively in the F₁₆ generation. In the homozygous strain, insecticide resistance was still stable and amplification of esterase remained after 16 generations under relaxation of insecticide selection, which indicated that copy of esterase gene was not lost in the offspring of this strain. The evolution of insecticide resistance in mosquitoes is discussed.     

Molluscicidal and Leishmanicidal Activity of the Leaf Essential Oil of Syzygium cumini (L.) Skeels from Brazil

The chemical composition and biological potential of the essential oil extracted from Syzygium cumini leaves collected in Brazil were examined. GC/MS Analyses revealed a high abundance of monoterpenes (87.12%) in the oil. Eleven compounds were identified, with the major components being α‐pinene (31.85%), (Z)‐β‐ocimene (28.98%), and (E)‐β‐ocimene (11.71%). To evaluate the molluscicidal effect of the oil, it was tested against Biomphalaria glabrata and the LC₅₀ obtained was 90 mg/l. The essential oil also showed significant activity against Leishmania amazonensis, with an IC₅₀ value equal to 60 mg/l. In addition, to evaluate its toxicity towards a non‐target organism, the essential oil was tested against Artemia salina and showed a LC₅₀ of 175 mg/l. Thus, the essential oil of S. cumini showed promising activity as a molluscicidal and leishmanicidal agent and might be valuable in combating neglected tropical diseases such as schistosomiasis and leishmaniasis. Further research is being conducted with regard to the purification and isolation of the most active essential‐oil compounds.     

Lethal and sub‐lethal effects of spinosad on the life‐history traits of army worm,Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), and its fitness cost of resistance

Spodoptera litura is one of the most destructive polyphagous insect pests, with more than 120 host‐plant species. In our present study, a field‐collected population of S. litura when selected with spinosad for 11 consecutive generations resulted in the development of 3921‐fold resistance to spinosad as compared to the susceptible strain. The spinosad‐resistant strain of S. litura had a relatively high fitness cost (0.17) as compared to the susceptible strain. Furthermore, the lethal and sub‐lethal effects of different concentrations of spinosad were checked on the susceptible strain at different levels; i.e., LC₄₀, LC₃₀, LC₂₀ and LC₁₀, which revealed that the impact of spinosad on the life‐history traits of S. litura increased with the increase in concentration of spinosad. A significant impact of spinosad was recorded on the larval duration, pre‐pupal weight, pupal duration, pupal weight, reproductive potential and adult emergence. The outcomes of the current research clearly indicate that fitness cost of spinosad and its sub‐lethal effects have a significant impact on population dynamics of S. litura, for which it can be incorporated in integrated pest management.     

Highly Toxic and Broad-Spectrum Insecticidal Bacillus thuringiensis Engineered by Using the Transposon Tn917 and Protoplast Fusion

The chromosome of the Bacillus thuringiensis strain S184 that was toxic against the third instar larvae of Spodoptera litura with the LC₅₀ of 9.74 μg/ml was successfully integrated into two genes of cyt1Aa and cry11Aa using the transposon Tn917, yielding the primary engineered strain TnX. The strain TnX was highly toxic to the third instar larvae of Culex pipiens fatigans with the LC₅₀ of 5.12 ng/ml which was 1.82-fold higher than that of B. thuringiensis subsp. israelensis, but lowly toxic to lepidopterous larvae. By the protoplast fusion of the strain TnX and the strain S184-Tet^r (resistance to tetracycline), the target engineered strain TnY was obtained. Against the third instar larvae of S. litura, the strain TnY LC₅₀ was of 4.68 μ g/ml and increased by 2.08-fold in comparison with the parent strain S184. Against the third instar larvae of C. pipiens fatigans, the strain TnY LC₅₀ was of 103.20 ng/ml. The two target genes of cyt1Aa and cry11Aa integrated into the chromosome were extremely stable and had little possibility of a second transposition. It was unclear whether some factors existing in the parent strain, S184, contributed to the high toxicity of the strains TnX and TnY. Received: 30 November 2000 / Accepted: 10 January 2001     

The effect of oxygen tension on the production of Bacillus thuringiensis subsp. israelensis toxin active against Aedes aegypti larvae

An optimized batch production of Bacillus thuringiensis subsp. israelensis was made in a stirred Bioflo III reactor using a previously selected medium, and operating conditions in the range of 100–500 rev/min stirrer speeds and 0.2–1 air flow/culture medium volume/minute (v/v/m) aeration rates, including five combinations; at the end of fermentation, dry powders were recovered and evaluated against Aedes aegypti larvae at 0.05 mg/l. Later, the lethal concentration inducing 50% mortality (LC₅₀) was determined for the two most toxic powders. The bioinsecticide yields varied from 9.1 to 15.7 g/l and the total fermentation times ranged between 18 and 30.3 h. The toxicity varied for two powders much more than for the others. These were for combinations with 300 rev/min:1 v/v/m and 500 rev/min:0.6 v/v/m, giving mortality percentages of 47.2 and 59.7, with LC₅₀ values of 0.2675 and 0.0685 mg/l, respectively. A t test showed no significant difference. However, the larvicidal powder produced with 300 rev/min:1 v/v/m gave more variable toxicity than the powder obtained with 500 rev/min:0.6 v/v/m.     

Identification and Kinetic Characteristics of an Indigenous Diesel-degrading Gordonia alkanivorans Strain

Summary An indigenous strain Gordonia alkanivorans CC-JG39 was isolated from oil-contaminated sludge of a local gas station located in central Taiwan. The bacterial isolate was able to grow on diesel-containing Bushnell–Haas medium and also tolerate various chemical additives frequently used in petroleum products (e.g. BETX, methyl-tert-butyl ether, and naphthalene). Kinetics of diesel-limited cell growth and biodegradation of diesel followed a Monod-type model. The kinetic constants for cell growth (μ_max and K_S,G) were 0.158 h⁻¹ and 3196 mg/l, respectively, while those for biodegradation of diesel (v_{max, diesel} and K_S,D) were 3.59 mg/h/mg cell and 2874 mg/l, respectively. G. alkanivorans CC-JG39 produced extracellular surface-active material, leading to a low surface tension of nearly 33 mN/m. The CC-JG39 strain also possessed the ability to float towards the oil/water interface. These features might play some roles in enhancing the mass transfer efficiency between oil substrate and the bacterial cells. Therefore, G. alkanivorans CC-JG39 may have potential applications in bioremediation of oil pollution sites.     

Genetics of resistance to Cry1C toxin from Bacillus thuringiensis in Spodoptera litura (Fab.)

The present study was undertaken to determine the genetics of Cry1C resistance in Spodoptera litura. Selection of S. litura (Fab.) with Cry1C was done for eight generations to develop resistance. Reciprocal crosses between resistant and susceptible populations were made to understand the population genetics of Cry1C resistance in S. litura. Generation wise selection with Cry1C was evaluated for resistance development in S. litura. The LC₅₀ of Cry1C was 0.14 µg/cm² for the first selected generation and it increased to 23.98 µg/cm² after eight selected generations, which is a 285.47-fold increase in resistance compared with the susceptible strain. The estimated realized heritability (h²) after eight generations of selection with Cry1C insecticidal protein was 0.44. The number of generations required for the tenfold increase in LC₅₀ (1/R) was estimated to be 3.33. Response to Cry1C selection in S. litura was 0.30, the estimated selection differential was 0.69 and the pheonotypic standard deviation (dP) was 0.24. Reciprocal crosses between Cry1C resistant and susceptible strain of S. litura showed autosomal resistance.     

Effects of B-azolemiteacrylic on life-history traits and demographic parameters of two-spotted spider mite,Tetranychus urticae (Acari: Tetranychidae)

The present study was conducted to evaluate sublethal effects of B-azolemiteacrylic on the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Female adults of T. urticae were exposed to LC₁₀ and LC₃₀ of the acaricide, and the effects on treated females and their offspring were evaluated. The results showed that the fecundity of F₀ female adults treated with LC₁₀ and LC₃₀ of B-azolemiteacrylic was reduced by 30.9 and 39.2%, respectively. Longevity and oviposition period of the females were significantly reduced as well. The developmental duration of egg and deutonymph stage of the F₁ generation were not significantly different from that of the control. The protonymph stage after LC₃₀ treatment lasted significantly longer, whereas the larva, deutonymph and female stage were significantly shorter than the control. The oviposition period of the F₁ generation was significantly shortened, the fecundity of each female decreased significantly, and the ratio of female-to-male was reduced too. Moreover, the average generation period of T. urticae after LC₁₀ and LC₃₀ treatments was shorter than that of the control, and the net production rate (R₀), intrinsic rate of increase (r_m) and finite rate of increase (λ) were all reduced by 33.3, 7.5 and 1.9% (LC₁₀ treatment) and by 51.3, 14.8 and 3.6% (LC₃₀ treatment), respectively. The population doubling time was prolonged by 7.5 and 14.8% after LC₁₀ and LC₃₀ treatments, respectively, compared with the control. These results indicate that B-azolemiteacrylic may effectively inhibit the development rate of the F₀ and F₁ populations of T. urticae, which will help design integrated strategies for the comprehensive control of T. urticae and rational use of pesticides in the field.