共查询到20条相似文献,搜索用时 15 毫秒
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Scott L. Allen Russell Bonduriansky Carla M. Sgro Stephen F. Chenoweth 《Molecular ecology》2017,26(5):1256-1272
Sex‐dependent gene expression is likely an important genomic mechanism that allows sex‐specific adaptation to environmental changes. Among Drosophila species, sex‐biased genes display remarkably consistent evolutionary patterns; male‐biased genes evolve faster than unbiased genes in both coding sequence and expression level, suggesting sex differences in selection through time. However, comparatively little is known of the evolutionary process shaping sex‐biased expression within species. Latitudinal clines offer an opportunity to examine how changes in key ecological parameters also influence sex‐specific selection and the evolution of sex‐biased gene expression. We assayed male and female gene expression in Drosophila serrata along a latitudinal gradient in eastern Australia spanning most of its endemic distribution. Analysis of 11 631 genes across eight populations revealed strong sex differences in the frequency, mode and strength of divergence. Divergence was far stronger in males than females and while latitudinal clines were evident in both sexes, male divergence was often population specific, suggesting responses to localized selection pressures that do not covary predictably with latitude. While divergence was enriched for male‐biased genes, there was no overrepresentation of X‐linked genes in males. By contrast, X‐linked divergence was elevated in females, especially for female‐biased genes. Many genes that diverged in D. serrata have homologs also showing latitudinal divergence in Drosophila simulans and Drosophila melanogaster on other continents, likely indicating parallel adaptation in these distantly related species. Our results suggest that sex differences in selection play an important role in shaping the evolution of gene expression over macro‐ and micro‐ecological spatial scales. 相似文献
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By using differential display PCR (DD-PCR) technique, two salt-inducible and one salt-repressed cDNA fragments were isolated
from rice. The three cDNA fragments were characterized respectively as partial sequence of rice S-adenosylmethionine decarboxylase
(SAMDC) gene, a new member of translation elongation factor 1A gene (namedREF1 A), and a novel gene whose function is unknown (namedSRG1). The full-length cDNA of SAMDC gene (namedSAMDC1) was further isolated by RT-PCR approach and the deduced polypeptide was found to be homologous to SAMDC proteins of other
plants, yeast and buman. Northern hybridization revealed that expression of SAMDCl and REFlA was induced, while SRGl was dramatically repressed, by salinity stress. Southern blot analysis demonstrated that SAMDCl and
SRGl were present as a single copy gene in rice genome, whereas riceREF1 A gene was organized as a gene family. TheREF1 A,SAMDC1, andSRG1 genes were located on chromosome 3,4, and 6 respectively by RFLP mapping approach using ZYQ8/JX17 DH population and RFLP
linkage maps.
Project supported by the National “863” High-Technology Program. 相似文献
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Background
Interspecific hybrids of frogs of the genus Xenopus result in sterile hybrid males and fertile hybrid females. Previous work has demonstrated a dramatic asymmetrical pattern of misexpression in hybrid males compared to the two parental species with relatively few genes misexpressed in comparisons of hybrids and the maternal species (X. laevis) and dramatically more genes misexpressed in hybrids compared to the paternal species (X. muelleri). In this work, we examine the gene expression pattern in hybrid females of X. laevis × X. muelleri to determine if this asymmetrical pattern of expression also occurs in hybrid females. 相似文献6.
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The evolution of the hedgehog gene family in chordates: insights from amphioxus hedgehog 总被引:9,自引:0,他引:9
Sebastian M. Shimeld 《Development genes and evolution》1999,209(1):40-47
The hedgehog family of intercellular signalling molecules have essential functions in patterning both Drosophila and vertebrate embryos. Drosophila has a single hedgehog gene, while vertebrates have evolved at least three types of hedgehog genes (the Sonic, Desert and
Indian types) by duplication and divergence of a single ancestral gene. Vertebrate Sonic-type genes typically show conserved
expression in the notochord and floor plate, while Desert- and Indian-type genes have different patterns of expression in
vertebrates from different classes. To determine the ancestral role of hedgehog in vertebrates, I have characterised the hedgehog
gene family in amphioxus. Amphioxus is the closest living relative of the vertebrates and develops a similar body plan, including
a dorsal neural tube and notochord. A single amphioxus hedgehog gene, AmphiHh, was identified and is probably the only hedgehog family member in amphioxus, showing the duplication of hedgehog genes to
be specific to the vertebrate lineage. AmphiHh expression was detected in the notochord and ventral neural tube, tissues that express Sonic-type genes in vertebrates. This
shows that amphioxus probably patterns its ventral neural tube using a molecular pathway conserved with vertebrates. AmphiHh was also expressed on the left side of the pharyngeal endoderm, reminiscent of the left-sided expression of Sonic hedgehog in chick embryos which forms part of a pathway controlling left/right asymmetric development. These data show that notochord,
floor plate and possibly left/right asymmetric expression are ancestral sites of hedgehog expression in vertebrates and amphioxus.
In vertebrates, all these features have been retained by Sonic-type genes. This may have freed Desert-type and Indian-type
hedgehog genes from selective constraint, allowing them to diverge and take on new roles in different vertebrate taxa.
Received: 20 July 1998 / Accepted: 23 September 1998 相似文献
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用差异显示法分析不同生长环境中的造血干细胞/祖细胞基因表达的初步研究 总被引:1,自引:0,他引:1
对比分析不同生长环境中的脐血CD34+造血干/祖细胞基因表达变化。方法: 采用静态和动态培养系统培养脐血单个核细胞,1周后收获CD34+造血干/祖细胞, 提取总RNA, 用差异显示法对比分析在不同生长环境中造血干/祖细胞基因表达的差异。 结果: 在所使用的差异显示条件下,得到30个差异表达基因片段,其中一个差异表达片段为RAN基因,该基因属于RAS癌基因家族,可能与造血细胞增殖有关。结论: 不同生长环境影响CD34+造血干/祖细胞的基因表达,这些差异表达的基因可为优化体外培养环境,扩增造血细胞提供分子基础。 相似文献
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The AP1/FUL clade of MADS box genes have undergone multiple duplication events among angiosperm species. While initially identified as
having floral meristem identity and floral organ identity function in Arabidopsis, the role of AP1 homologs does not appear to be universally conserved even among eudicots. In comparison, the role of FRUITFULL has not been extensively explored in non-model species. We report on the isolation of three AP1/FUL genes from cultivated spinach, Spinacia oleracea L. Two genes, designated SpAPETALA1-1 (SpAP1-1) and SpAPETALA1-2 (SpAP1-2), cluster as paralogous genes within the Caryophyllales AP1 clade. They are highly differentiated in the 3′, carboxyl-end encoding region of the gene following the third amphipathic
alpha-helix region, while still retaining some elements of a signature AP1 carboxyl motifs. In situ hybridization studies also demonstrate that the two paralogs have evolved different temporal and
spatial expression patterns, and that neither gene is expressed in the developing sepal whorl, suggesting that the AP1 floral organ identity function is not conserved in spinach. The spinach FRUITFULL homolog, SpFRUITFULL (SpFUL), has retained the conserved motif and groups with Caryophyllales FRUITFULL homologs. SpFUL is expressed in leaf as well as in floral tissue, and shows strong expression late in flower development, particularly in
the tapetal layer in males, and in the endothecium layer and stigma, in the females. The combined evidence of high rates of
non-synonymous substitutions and differential expression patterns supports a scenario in which the AP1 homologs in the spinach AP1/FUL gene family have experienced rapid evolution following duplication.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Rachid Safi Agnès Begue Catherine Hänni Dominique Stehelin Jamshed R. Tata Vincent Laudet 《Journal of molecular evolution》1997,44(6):595-604
Since thyroid hormones play a pivotal role in amphibian metamorphosis we used PCR to amplify DNA fragments corresponding
to a portion of the ligand-binding domain of the thyroid hormone receptor (TR) genes in several neotenic amphibians: the obligatory
neotenic members of the family Proteidea the mudpuppy Necturus maculosus and Proteus anguinus as well as two members of the facultative neotenic Ambystoma genus: the axolotl Ambystoma mexicanum and the tiger salamander Ambystoma tigrinum. In addition, we looked for TR genes in the genome of an apode Typhlonectes compressicaudus. TR genes were found in all these species including the obligatory neotenic ones. The PCR fragments obtained encompass both
the C and E domains and correspond to α and β genes. Their sequences appear to be normal, suggesting that there is no acceleration
of evolutionary rates in the TR genes of neotenic amphibians. This result is not surprising for Ambystomatidae, which are
known to respond to T3 (3,3′,5-triiodothyronine) but is not in agreement with biochemical and biological data showing that
Proteidea cannot respond to thyroid hormones. Interestingly, by RT-PCR analysis we observed a high expression levels of TRα
in gills, intestine, and muscles of Necturus as well as in the liver of Ambystoma mexicanum, whereas TRβ expression was only detected in Ambystoma mexicanum but not in Necturus. Such a differential expression pattern of TRα and TRβ may explain the neoteny in Proteidea. The cloning of thyroid-hormone-receptor
gene fragments from these species will allow the molecular study of their failure to undergo metamorphosis.
Received: 23 April 1996 / Accepted: 20 January 1997 相似文献
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The mate recognition protein (MRP) gene is a member of a family of extracellular matrix protein genes, called MRP Motif Repeat
(MMR) genes, with no known homologs. Two sets of MMR genes, designated MMR-A and MMR-B, were found in Brachionus manjavacas. MMR-B has previously been shown to encode the MRP in the Brachionus plicatilis species complex. MMR family genes share the same basic structure: a signal peptide sequence, followed by nearly identical
276 bp (MMR-A) or 261 bp (MMR-B) repeats, with a truncated final repeat. Each repeat of the predicted MMR-A and -B proteins
is expected to have a secondary structure of 5 α-helices, ranging in length from 11 to 20 amino acids, separated by coils
of 1–3 amino acids. Hydrophobic and hydrophilic amino acids are predicted to be partitioned to opposite sides of each α-helix,
suggesting that MMR proteins are globular with a hydrophobic core. MMR-A and MMR-B proteins vary in their post-translational
modifications, resulting in differences in size and charge, and likely causing differences in the physical properties of the
proteins on the surface of the female, and their ability to be recognized by a receptor on a male rotifer. The identity of
MMR gene repeats is theorized to be maintained by concerted evolution, through a process of unequal crossing over and/or gene
conversion, with new mutations likely to be lost. Rarely, however, the same process of concerted evolution can rapidly spread
a mutation across all of the repeats. When a mutation results in conformational changes in the protein detectable by males,
it could lead to reproductive isolation and thereby to speciation. Thus, changes in MRP could be a driving force in the high
degree of species diversity seen within the B. plicatilis cryptic species complex. 相似文献
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Differential expression of manganese superoxide dismutase sequence variants in near isogenic lines of wheat during cold acclimation 总被引:7,自引:0,他引:7
Numerous sequence variants of wheat (Triticum aestivum L.) manganese superoxide dismutase (MnSOD) genes have been found. Quantitative real-time PCR was used to measure the expression
levels of three MnSOD genes distinguished by a variable amino acid, and three genes distinguished by sequence variation in
the 3′ untranslated region (3′ UTR), in wheat plants grown at 20°C and cold-acclimated for 1–4 weeks at 2°C. The amino acid
variants did not differ significantly in expression levels, however, differential expression of genes differing in the 3′
UTR was observed. Diploid wheat-related species also carried sequence variants of MnSOD, with differing levels of expression,
suggesting diversification of the MnSOD gene family occurred prior to the polyploidization events of hexaploid wheat. 相似文献
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Zhong Q Zhang Q Wang Z Qi J Chen Y Li S Sun Y Li C Lan X 《Marine biotechnology (New York, N.Y.)》2008,10(3):310-318
Differential expression of genes is crucial to embryogenesis. The analysis of gene expression requires appropriate references
that should be minimally regulated during the embryonic development. To select the most stable genes for gene normalization,
the expression profiles of eight commonly used reference genes (ACTB, GAPDH, rpL17, α-Tub, EF1-α, UbcE, B2M, and 18S rRNA)
were examined during Japanese flounder (Paralichthys olivaceus) embryonic development using quantitative real-time polymerase chain reaction. It was found that all seven mRNA genes appeared
to be developmentally regulated and exhibited significant variation of expression. However, further analyses revealed the
stage-specific expression stability. Hence when normalization using these mRNA genes, the differential and stage-related expression
should be considered. 18S rRNA gene, on the other hand, showed the most stable expression and could be recommended as a suitable
reference gene during all embryonic developmental stages in P. olivaceus. In summary, our results provided not only the appropriate reference gene for embryonic development research in P. olivaceus, but also possible guidance to reference gene selection for embryonic gene expression analyses in other fish species. 相似文献
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Ghiselli F Milani L Chang PL Hedgecock D Davis JP Nuzhdin SV Passamonti M 《Molecular biology and evolution》2012,29(2):771-786
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We searched for sex-specific DNA sequences in the male and female genomes of African catfish, Clarias gariepinus (Burchell, 1822) by comparative random amplified polymorphic DNA (RAPD) assays performed on pooled DNA samples. Two sex-linked
RAPD markers were identified from the male DNA pool and confirmed on individual samples, showing good agreement with phenotypic
sex. Both markers were isolated, cloned and characterized. The first marker (CgaY1) was nearly 2.6 kb long, while the length
of second one (CgaY2) was 458 bp. Southern blot analysis with a CgaY1 probe showed strong hybridizing fragments only in males
and not in females under stringent conditions, indicating the presence of multiple copies of CgaY1 in the male genome. When
tested by zoo blot on the genomes of two closely related species from the Clariidae family, CgaY1 hybridized to the DNA of Heterobranchus longifilis and generated a faint male-specific band at low stringency. CgaY2 produced similar hybridization pattern in both sexes of
C. gariepinus, C. macrocephalus and H. longifilis. Specific primers were designed to the sequences and the markers were amplified in multiplex PCR reactions together with
a control band common to all individuals. This allowed for rapid, molecular sexing of the species on the basis of a simple
three band (male) versus one band (female) pattern. According to our knowledge these are the first sex-specific DNA markers
isolated from a siluroid fish species.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Novak AE Jost MC Lu Y Taylor AD Zakon HH Ribera AB 《Journal of molecular evolution》2006,63(2):208-221
Voltage-gated sodium channels underlie action potential generation in excitable tissue. To establish the evolutionary mechanisms
that shaped the vertebrate sodium channel α-subunit (SCNA) gene family and their encoded Nav1 proteins, we identified all SCNA genes in several teleost species. Molecular cloning revealed that teleosts have eight SCNA genes, compared to ten in another vertebrate lineage, mammals. Prior phylogenetic analyses have indicated that the genomes
of both teleosts and tetrapods contain four monophyletic groups of SCNA genes, and that tandem duplications expanded the number of genes in two of the four mammalian groups. However, the number
of genes in each group varies between teleosts and tetrapods, suggesting different evolutionary histories in the two vertebrate
lineages. Our findings from phylogenetic analysis and chromosomal mapping of Danio rerio genes indicate that tandem duplications are an unlikely mechanism for generation of the extant teleost SCNA genes. Instead, analyses of other closely mapped genes in D. rerio as well as of SCNA genes from several teleost species all support the hypothesis that a whole-genome duplication was involved in expansion of
the SCNA gene family in teleosts. Interestingly, despite their different evolutionary histories, mRNA analyses demonstrated a conservation
of expression patterns for SCNA orthologues in teleosts and tetrapods, suggesting functional conservation.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users.
[Reviewing Editor: Dr. Axel Meyer] 相似文献
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Expression pattern analysis has been revealed that glutathione S-transferase omega 2 (GSTO2, a member of class omega) is ubiquitously expressed. Over expression of GSTO2 induced apoptosis.
The gene encoding GSTO2 was localized to human chromosome 10q24.3, a region that may harbor gene(s) involved in the developing of colorectal cancer.
To investigate the association between GSTO2 N142D genetic polymorphism and susceptibility to colorectal cancer the present study was done. We studied 63 (26 females,
37 males) colorectal cancer patients and 126 (52 females, 74 males) healthy individuals. The control subjects were frequency
matched for age and gender with the colorectal cancer group. The genotypes were performed using RFLP-PCR method. The ND and
DD genotypes were not associated with risk of colorectal cancer, in comparison with the NN genotype. Family history for cancer
in the first degree of relatives significantly differed between cases and controls (P = 0.012). The profiles of GSTO2 genotypes and family history in control and cancerous groups were compared to each other. Subjects with NN genotype and positive
family history significantly were at high risk to develop colorectal cancer in comparison with subjects with DD or ND genotypes
and negative family history (P = 0.003). Present findings indicating that GSTO2 NN genotype increase the risk of colorectal cancer in persons with positive family history for cancer in the first degree
relatives. 相似文献