A new species of Sicyos (Cucurbitaceae, Sicyeae, Sicyinae) from Mexico and Guatemala

Sicyos lirae is described as a new species from Chiapas, Mexico, and Guatemala. It grows in zones with primary or secondary montane rain forest at elevations between 1400 and 3800 meters. In Guatemala the species is commonly called “güisquil de ratón.” It is similar toSicyos galeottii Cogn., from which it differs in blade consistency, shape of the stigma lobes, and several important ovary and fruit characters.     

Endopolyploidy and structures of the nuclei in the endosperm of theCucurbitaceae

Summary In the endosperm ofEchinocystis lobata the endopolyploid nuclei show structural changes of chromocentres typical of the process of endomitosis in Angiosperms.The cytological differentiation of the endosperm proper and the chalazal haustorium in three further representatives of theCucurbitaceae: Sicyos angulata, Cyclanthera pedata andCitrullus colocynthis is also connected with endomitotic polyploidisation. The nuclei of the endosperm proper attain in the different species different levels of polyploidy, 384n, as a maximum. In the nuclei of the chalazal haustorium the degree of polyploidy is lower (as a maximum 24n).The structure of polyploid nuclei which did not undergo postendomitotic mitoses shows some differences in the species studied in the course of the present work.Sicyos angulata has prochromosomic diploid nuclei, whereas those ofCitrullus colocynthis andCyclanthera pedata can be assigned to chromomeric nuclei with a dense structure. Endopolyploid nuclei of all three species studied are characterized by the occurence of compact chromocentres of different size; besides in some nuclei ofSicyos angulata andCyclanthera pedata the chromocentres within the resting nucleus reveal their chromomere structure. In addition, inCitrullus colocynthis nuclei with numerous thin threads of various degrees of spiralisation have been observed. — In all three species the endopolyploid nuclei after mitotic divisions have notably higher numbers of nucleoli and small chromocentres.Dedicated to Professor L.Geitler on the occasion of his 70th birthday.     

First Report of Cercospora Leaf Spot of Burcucumber Caused by Cercospora citrullina in Korea

Burcucumbers (Sicyos angulatus) showing necrotic leaf spots were found in several locations in Korea during 2008–2012. The causal agent was a fungus that was identified as Cercospora citrullina, based on morphological characteristics as well as sequences of the ribosomal DNA internal transcribed spacer region. Although C. citrullina has been known to attack various cucurbitaceous plants, this is the first report of this fungus on S. angulatus.     

Nomenclatural changes in <Emphasis Type="Italic">Lithospermum</Emphasis> (Boraginaceae) and related taxa following a reassessment of phylogenetic relationships

Lithospermum (Boraginaceae) comprises approximately 40 species in both the Old and New Worlds, with a center of diversity in the southwestern United States and Mexico. Using ten cpDNA regions, a phylogeny of Lithospermum and related taxa was reconstructed. Lithospermum (including New World and Old World species) and related New World members of Lithospermeae form a monophyletic group, with Macromeria, Onosmodium, Nomosa, Lasiarrhenum, and Psilolaemus nested among species of Lithospermum. New World Lithospermeae also is a monophyletic group, with Eurasian species of Lithospermum sister to this group. Because Lithospermum is not monophyletic without the inclusion of the other New World genera, species from these genera are transferred to Lithospermum, and appropriate nomenclatural changes are made. New combinations are Lithospermum album, Lithospermum barbigerum, Lithospermum dodrantale, Lithospermum exsertum, Lithospermum helleri, Lithospemum leonotis, Lithospermum notatum, Lithospermum oaxacanum, Lithospermum pinetorum, Lithospermum rosei, Lithospermum trinverium, and Lithospermum unicum; new names are Lithospermum chiapense, Lithospermum johnstonii, Lithospermum macromeria, Lithospermum onosmodium, Lithospermum rzedowskii, and Lithospermum turneri.     

Phylogenetic relationships of species in <Emphasis Type="Italic">Pseudoroegneria</Emphasis> (Poaceae: Triticeae) and related genera inferred from nuclear rDNA ITS (internal transcribed spacer) sequences

To evaluate the phylogenetic relationships of species in Pseudoroegneria and related genera, the nuclear ribosomal internal transcribed spacer (ITS) sequences were analyzed for eighteen Pseudoroegneria (St), two Elytrigia (E ^e St), two Douglasdeweya (StP), three Lophopyrum (E ^e and E ^b ), three Agropyron (P), two Hordeum (H), two Australopyrum (W) and two Psathyrostachys (Ns) accessions. The main results were: (i) Pseudoroegneria gracillima, P. stipifolia, P. cognata and P. strigosa (2x) were in one clade, while P. libanotica, P. tauri and P. spicata (2x) were in the other clade, indicating there are the differentiations of St genome among diploid Pseudoroegneria species; (ii) P. geniculata ssp. scythica, P. geniculata ssp. pruinifera, Elytriga caespitosa and Et. caespitosa ssp. nodosa formed the E ^e St clade with 6-bp indel in ITS1 regions; and (iii) Douglasdeweya wangii, D. deweyi, Agropyron cristatum and A. puberulum comprised the P clade. It is unreasonable to treat P. geniculata ssp. scythica and P. geniculata ssp. pruinifera as the subspecies of P. geniculata, and they should be transferred to a new genus Trichopyrum, which consists of species with E ^e St genomes. It is also suggested that one of the diploid donor of D. wangii and D. deweyi is derived from Agropyron species, and it is reasonable to treat tetraploid species with StP genomes into Douglasdeweya.     

Novelties in spermacoceae (Rubiaceae) from Bolivia and Paraguay

One new Bolivian species (Borreria pazensis) and three new varieties (Borreria densiflora var.minima, Richardia, scabra var.chacoensis andStaelia virgata var.killeenii) are described and illustrated. The genusDiodella, with three species (D. apiculata comb. nov., D. radula comb. nov., andD. teres), is reported for the first time.Borreria limae, B. wunschmannii, B. scabiosoides var.scabiosoides, andB. scabiosoides var.anderssonii are also reported as new to Bolivia.     

A revision of Casimirella,including Humirianthera (Icacinaceae)

Casimirella Hassler (1913) is accepted and Humirianthera Huber (1914) considered a synonym. Casimirella diversifolia and C. lanata from Brazil are described as new species. Casimirella ampla (Miers) based on Leretia ampla Miers, C. crispula (Howard) based on Humirianthera crispula Howard, and C. rupestris (Ducke) based on Humirianthera rupestris Ducke are new combinations.     

Genome analysis of species in the genus Hystrix (Triticeae; Poaceae)

Genomic in situ hybridisation (GISH) and Southern genomic hybridisation were applied in order to gain further knowledge regarding generic delimitation of the genus Hystrix as well as to clarify the genomes of the Hystrix species H. patula, H. longearistata, H. coreana, H. duthiei and H. komarovii. The hybridisation intensity of different genomic probes was compared among the Hystrix species and with other Triticeae species. The Southern- and GISH results confirm that H. patula contains the StH genome and show that H. komarovii most likely has a variant of this StH genome. The other Hystrix species under study, i.e. H. longearistata, H. coreana and H. duthiei, contain an Ns basic genome, and most probably two variants of this basic genome, Ns ¹ Ns ² . The genus Hystrix is thus not a monophyletic group of species.     

Hydroxylation of steroids by Fusarium oxysporum, Exophiala jeanselmei and Ceratocystis paradoxa

The potential of Fusarium oxysporum var. cubense UAMH 9013 to perform steroid biotransformations was reinvestigated using single phase and pulse feed conditions. The following natural steroids served as substrates: dehydroepiandrosterone (1), pregnenolone (2), testosterone (3), progesterone (4), cortisone (5), prednisone (6), estrone (7) and sarsasapogenin (8). The results showed the possible presence of C-7 and C-15 hydroxylase enzymes. This hypothesis was explored using three synthetic androstanes: androstane-3,17-dione (9), androsta-4,6-diene-3,17-dione (10) and 3α,5α-cycloandrost-6-en-17-one (11). These fermentations of non-natural steroids showed that C-7 hydroxylation was as a result of that position being allylic. The evidence also pointed towards the presence of a C-15 hydroxylase enzyme.The eleven steroids were also fed to Exophialajeanselmei var. lecanii-corni UAMH 8783. The results showed that the fungus appears to have very active 5α and 14α-hydroxylase enzymes, and is also capable of carrying out allylic oxidations.Ceratocystis paradoxa UAMH 8784 was grown in the presence of the above-mentioned steroids. The results showed that monooxygenases which effect allylic hydroxylation and Baeyer–Villiger rearrangement were active. However, redox reactions predominated.     

猫爪草中的脂肪酸类化合物

为了解小毛茛(Ranunculus ternatus Thunb.)的化学成分,采用色谱技术从其干燥块根猫爪草中分离纯化得到5个脂肪酸类化合物,经波谱分析,他们的结构分别鉴定为(R)-3-hydroxy-11-methoxy-11-oxoundecanoic acid(1)、十六烷酸(2)、棕榈酸乙酯(3)、已二酸(4)和硬脂酸(5)。其中,化合物1为新化合物,这些成分对耐药结核分枝杆菌(耐INH+RFP)有一定的体外抑制活性。     

A phylogeny of Cariniana (Lecythidaceae) based on morphological and anatomical data

Cariniana as previously circumscribed is a genus of 16 species restricted to neotropical forest habitats on well-drained sites. A phylogenetic analysis of the genus based on 33 morphological and anatomical characters was undertaken. The results show that Cariniana consists of two clades: the Allantoma/Cariniana decandra clade includes Allantoma lineata and seven species of actinomorphic-flowered Cariniana and is characterized by 5-merous flowers, carnose petals, incurved petal apex, scarcely lobed calyces, eucamptodromous secondary veins, dichotomizing venation, and poorly developed areolation; the C. legalis clade is made up of nine species and is characterized by an obliquely zygomorphic androecium, reticulate tertiary venation, and anomocytic stomata. The actinomorphic-flowered Cariniana are more closely related to the monotypic Allantoma lineata than they are to the species of the C. legalis clade. In order to reflect these relationships, Cariniana is divided into two genera: species in the C. legalis clade, which includes the generic type C. legalis, remain as Cariniana while species of Cariniana in the Allantoma/Cariniana decandra clade are transferred to Allantoma. The following new combinations are proposed: Allantoma decandra, A. integrifolia, A. kuhlmannii, A. pluriflora (a nomen novum for Cariniana multiflora because Allantoma multiflora is a synonym of Couratari multiflora), A. pachyantha, A. pauciramosa, and A. uaupensis.     

Two new species of Serjania (Sapindaceae) from southeastern Brazil

One new species ofSerjania (S. erythrocaulis) belonging to theS. paradoxa group ofSerjania sect.Eurycoccus is described and illustrated bringing the total number of species in this group to eight. A second new species,Serjania divaricocca, belonging to sect.Serjania is also described and illustrated.

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Resumen  Una especie neuva deSerjania (S. erythrocaulis) perteneciente al grupoS. paradoxa de la secciónEurycoccus es descrita e ilustrada. Con esta especie, el numero total de especies conocidas para este grupo aumenta a ocho. Una segunda nueva especie,Serjania divaricocca, perteneciente a la secciónSerjania es también descrita e ilustrada.

     

A new species of Sicyos (Cucurbitaceae) from Chiapas,Mexico

Sicyos motozintlensis is described from the vicinity of Motozintla de Mendoza, Chiapas. It shows a strong similarity toS. chiriquensis Hammel & D’Arcy from Panama.     

Revision of <Emphasis Type="Italic">Heterosavia</Emphasis>, stat. nov., with notes on <Emphasis Type="Italic">Gonatogyne</Emphasis> and <Emphasis Type="Italic">Savia</Emphasis> (Phyllanthaceae)

Heterosavia (Phyllanthaceae) is segregated from Savia (tribe Bridelieae), recognized at generic rank, and placed in tribe Phyllantheae. Floral, fruit, leaf anatomical, leaf venation, and pollen characters of the neotropical taxa previously united as Savia including Gonatogyne are discussed and illustrated. Keys to the three genera and to the species of Heterosavia are presented. Four species (all new combinations), Heterosavia bahamensis, H. erythroxyloides, H. laurifolia, and H. maculata, are recognized. The new combinations Heterosavia laurifolia var. intermedia and H. maculata var. clementis are proposed. The names Heterosavia, H. erythroxyloides, H. laurifolia, Savia clementis, S. clusiifolia, S. clusiifolia var. fallax, and S. longipes are lectotypified. Distribution maps and conservation assessments (IUCN ratings) of Heterosavia species and varieties are provided.     

采自吉林省敦化市的2个蘑菇目(Agaricales)新记录种

【背景】在中国吉林省延边朝鲜族自治州敦化市寒葱岭红叶谷进行大型真菌资源调查的过程中发现了2个物种。【目的】确定这2个物种的分类地位。【方法】采集大型真菌标本,对其形态进行详细观察和描述,并提取DNA、测定rDNA ITS序列,基于最大似然法和贝叶斯法构建分子系统发育树。【结果】鉴定结果表明,这2个蘑菇目物种分别是径边刺毛鬼伞(Tulosesus callinus)和拉氏红菇(Russula lakhanpalii),在形态上与原始描述高度吻合,系统发育分析也验证了形态学的鉴定结果。【结论】拉氏红菇(R. lakhanpalii)系中国首次报道,径边刺毛鬼伞(T. callinus)为吉林省新记录种。     

铁皮石斛内生真菌次生代谢产物研究

为了解铁皮石斛(Dendrobium officinale)内生真菌Phyllosticta aristolochiicola的次生代谢产物,从该真菌中分离得到15个化合物,经波谱分析分别鉴定为N-methyl-2-pyrolidinone (1)、环-(甘氨酸-L-脯氨酸)(2)、环-(D-丙氨酸-L-脯氨酸)(3)、环-(L-缬氨酸-L-脯氨酸)(4)、环-(L-亮氨酸-L-脯氨酸)(5)、cyclo-(L-Leu-D-4-hydroxyprolinyl)(6)、环-(L-苯丙氨酸-L-脯氨酸)(7)、环-(L-苯丙氨酸-L-4-羟基脯氨酸)(8)、环-(L-酪氨酸-L-脯氨酸)(9)、环-(L-苯丙氨酸-L-亮氨酸)(10)、啤酒甾醇(11)、对羟基苯乙醇(12)、对羟基苯乙酸(13)、(2S,3R)-1-(4-羟基苯基)丁烷-2,3-二醇(14)和(2R,3S)-1-苯基丁烷-2,3-二醇(15)。采用MTS法检测抗肿瘤活性表明,化合物2、10和14对HL-60、A-549、SMMC-7721、MCF-7和SW-480细胞株具有一定的抑制活性。     

假单胞菌T1-3-2的抑菌特性及其对杉木的抗病促生作用

【背景】生物防治是“基于自然的解决方案”,有利于生态文明和可持续发展,开展生物防治技术研究的基础是明确菌株的生防作用和抑菌特性。【目的】探究杉木内生菌株T1-3-2的抑菌促生特性,为研制该菌株生防菌剂、防治杉木炭疽病(Cunninghamia lanceolata anthracnose)奠定基础。【方法】通过形态学特征、生理生化特性及16S rRNA基因序列分析,确定菌株T1-3-2的分类地位;通过平板对峙、菌落径向生长抑制率和平板倒扣等方法测定细菌及其挥发性气体和次级代谢产物的抑菌作用;同时,测定其促生作用和室内防效。【结果】菌株T1-3-2与桉生假单胞菌(Pseudomonas eucalypticola)亲缘性较近,属于假单胞菌属。该菌株对分属于6个属的10株靶标菌株具有较强的拮抗作用,尤其对炭疽菌属、拟盘多毛孢属、黑孢霉属和葡萄座腔菌属的6株靶标菌株抑制率高达80%以上。室内盆栽试验显示：菌株T1-3-2用Kings Medium B液体培养基的发酵菌液对杉木炭疽病的防效可达74.20%,同时能有效改善杉木幼苗的生长状况、增加生物量。【结论】菌株T1-3-2隶属于假单胞菌属,对杉木具有良好的抗病促生作用,是一株具有开发潜力的生防菌株。     

托光柄菇属一中国新记录种

[背景] 托光柄菇属（Volvopluteus）隶属于光柄菇科（Pluteaceae）,目前世界上仅有4个种。[目的] 调查我国东北地区大型真菌资源。[方法] 采集大型真菌标本,对其形态进行详细的观察和描述,提取DNA,测定rDNA ITS序列,基于最大似然法和贝叶斯法构建系统发育树。[结果] 2019–2020年在吉林省延边朝鲜族自治州敦化市采集的标本中,有6份标本为密执安托光柄菇V.michiganensis,该种真菌此前未在我国发现。在系统发育分析中,采自我国的密执安托光柄菇V.michiganensis与该种的模式标本聚为一个分支。[结论] 密执安托光柄菇V.michiganensis为中国新记录种。     

Review of the neotropical endemics Burmeistera, Centropogon, and Siphocampylus (Campanulaceae: Lobelioideae), with description of 18 new species and a new section

Burmeistera, Centropogon, and Siphocampylus together comprise more than 500 species endemic to the Neotropics, Current knowledge of these genera is unsatisfactory, with much diversity yet to be described. Over the past seven years, one out of every 28 specimens received for identification has represented a new species, many characterized by unique or unusual character states. Siphocampylus smilax (Bolivia) is unique in the genus in its parallelodromous leaf venation and extra-floral nectaries. Centropogon dianae (Peru) cannot be accommodated in any recognized infrageneric taxon, and a new section, Centropogon sect. Niveopsis, is proposed for it. The flowers of C. steinii (Ecuador) are the smallest known in the genus, while C. connatilobatus (Venezuela) is the first species to combine arbusculiform pubescence and connate calyx lobes. Burmeistera venezuelensis is only the second species of the genus known to occur in Venezuela and the first to be endemic; similarly, Centropogon wilburii is only the third species of Centropogon known from Mexico and the first to be endemic to that country. Additional novelties are also described.     

Description of a new species and notes on <Emphasis Type="Italic">Crossoglossa</Emphasis> (Orchidaceae: Epidendroideae: Malaxideae) from the eastern Andes in Colombia and Venezuela

A new species of Crossoglossa (Orchidaceae: Epidendroideae: Malaxideae), Crossoglossa acuminatissima, from Santander del Sur Department in Colombia, is described and illustrated, and its affinities are discussed. Malaxis tipuloides var. exigua is raised to the rank of species as Crossoglossa exigua, and its report from Venezuela constitutes the first record of the genus Crossoglossa in that country.

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Resumen  Se describe Crossoglossa acuminatissima de Santander del Sur en Colombia y se provee una ilustración y una discusión de sus afinidades. Malaxis tipuloides var. exigua se eleva al rango de especie como Crossoglossa exigua, y su reporte para Venezuela constituye el primer registro del género en ese país.