共查询到20条相似文献,搜索用时 87 毫秒
1.
2.
3.
本文介绍了植物细胞培养的特点及其生物反应器的设计原理,概述了植物细胞悬浮培养和固定化细胞系统中各类生物反应器的传氧、混合和流体力学特性与植物细胞生长和次生代谢物生产的关系。 相似文献
4.
植物细胞生物反应器培养的研究进展(Ⅰ) 总被引:2,自引:1,他引:2
利用植物细胞大规模悬浮培养生产植物有用代谢产物在近些所来取得了很大发展,但植物细胞悬浮培养的工业化应用受到来自生物及工程技术上的限制,本文针对植物细胞培养的基本特点,详细讨论了与大规模生产有关的工程技术方面的问题,如植物细胞聚集,溶氧及气体成分,流体性能,剪切力对植物细胞培养产生的影响。 相似文献
5.
利用植物细胞生物反应器技术生产植物有用代谢产物,近些年来取得了很大发展,但植物细胞悬浮培养的工业化应用仍受到来自生物及工程技术上的限制。针对植物细胞生物反应器技术的特点及其研究进展,提出在综合考虑生物学和工艺学两方面的基础上,选育药用植物稳定高产的优良细胞系是提高植物细胞生物反应器技术可行性的关键。 相似文献
6.
生物反应器(bioreactor)是一种以表达目标产物或获得繁殖体为目的的设备系统,包括微生物、动物、植物生物反应器以及相关设备。植物生物反应器(phytobioreactor)是借鉴植物组织培养和微生物发酵原理制作的设备系统。其中,应用较广泛的是间歇浸没式植物生物反应器。与传统植物组织培养相比,该方法具备可换气、无需转接和大容量培养等特点。国内制作的BIOF系列新型植物生物反应器还可以利用串/并联方法,实现更高通量培养能力,其应用于植物种苗繁育、代谢产物的表达、耐盐等变异的定向筛选、植物生长发育的动态分析等方面均具备显著优势。现代植物生物技术在基础研究和产业方面的应用对植物生物反应器提出了新要求,新型生物反应器应用方法的持续改进和设备系统的不断完善,使其成为植物学领域的高效研究平台,并将促进植物育种和植物源化合物的发掘等方面研究效率的提高。 相似文献
7.
植物生物反应器研究进展 总被引:6,自引:0,他引:6
利用植物生物反应器生产外源蛋白具有许多优点 ,其吸引力与日俱增。转基因植物生物反应器已逐步成为国内外基因工程领域的研究热点之一。就其概念、特点和研究进展、存在的问题等作一综述。 相似文献
8.
利用植物细胞大规模悬浮培养生产植物有用代谢产物在近些年来取得了很大发展,但植物细胞悬浮培养的工业化应用受到来自生物及工程技术上的限制。本文针对植物细胞培养的基本特点,详细讨论了与大规模生产有关的工程技术方面的问题,如植物细胞聚集、溶氧及气体成分、流体性能、剪切力对植物细胞培养产生的影响。 相似文献
9.
植物组织培养生产有用次生代谢产物的研究进展 总被引:12,自引:0,他引:12
本文论述了植物组织培养生产次级代谢的研究进展,对一些主要问题进行了讨论和展望,包括:影响主次生代谢产物的因素、提高产量的途径、生物反应器技术的应用等。 相似文献
10.
利用转基因植物作为生物反应器表达重组蛋白,生产外源蛋白质作为动物疫苗是一个很有吸引力的廉价生产系统,它有可能代替生产成本较高的传统疫苗的发酵生产系统。通过口蹄疫病毒VPI结构蛋白基因在转基因植物中的表达,口蹄疫疫苗已在植物中产生。在植物中生产的抗原能够保持其自身的免疫原性。本文简要综述了近十年来用转基因植物作为生物反应器生产口蹄疫疫苗的研究进展、特点及其应用前景。 相似文献
11.
12.
13.
Commercialization of plant tissue culture in India 总被引:2,自引:0,他引:2
Commercial application of plant tissue culture started in USA with micropropagation of orchids in 1970s. It has seen tremendous
expansion globally from 1985 to 1990 in the number of production units as well as the number of plants produced. With an estimated
global market of 15 billion US dollars per annum for tissue cultured products, even with exponential expansion in the industry,
the demand far exceeds production, leaving enough scope for expansion. This industry appears to be undergoing a pause in growth
presently in developed countries as it is finding difficult to remain cost–effective. In US, only half the production capacity
is being utilized currently due to high labour costs. In developing countries, with lower wage scales, plants are being produced
at much cheaper rates. Indian micropropagation industry, though a late starter by almost a decade, compared to its western
counterparts, has expanded exponentially from 5 million annual capacity in 1988 to 190 million in 1996. The facilities now
created are at par with the best in leading countries like the Netherlands and USA. To remain in profitable business and to
earn the much needed foreign exchange, Indian units need to judiciously mix steady revenue generating items with unique speciality
items based on demand in domestic and international markets.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
14.
The influence of ethylene in plant tissue culture 总被引:14,自引:0,他引:14
N. L. Biddington 《Plant Growth Regulation》1992,11(2):173-187
Ethylene produced by plant tissues grown in vitro may accumulate in large quantities in the culture vessels, particularly from rapidly growing non-differentiated callus or suspension cultures, and hence is likely to influence growth and development in such systems. Research into this aspect of tissue culture has been sparse, although it has grown recently with the increasing importance of in vitro regeneration. This review deals with the measurement and relevance of the accumulated ethylene, and the influence of both exogenous and endogenous ethylene in the different types of tissue culture systems. The relationships between ethylene and other growth regulators in tissue culture growth and development are also discussed. Although in some cases its influence seems negligible, in many types of tissue culture ethylene may act either as a promoter or inhibitor depending on the species used. Thus ethylene has an important influence on many aspects of in vitro regeneration, but it is also clear that we cannot at present describe a specific role or roles for ethylene in tissue culture which can be applied at a general, species-wide level. If its effects are to be enhanced or diminished in order to improve the efficiency and range of plant tissue culture, then more research is needed to clarify what its fundamental role might be in in vitro growth and development.Abbreviations ABA
abscisic acid
- ACC
1-aminocyclopropane-1-carboxylic acid
- AOA
aminooxyacetic acid
- ASA
acetylsalicyclic acid
- AVG
aminoethoxyvinylglycine
- BA
N6 benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid
- DNP
2,4-dinitrophenol
- GA
gibberellin
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- NAA
naphthaleneacetic acid
- SAM
S-adenosylmethionine
- STS
silver thiosulphate
- TIBA
2,3,5-triidobenzoic acid 相似文献
15.
Ross H. Hall 《In vitro cellular & developmental biology. Plant》1976,12(3):216-224
Summary Cells possess extraordinary powers to organize their molecular processes not only to maintain a cell in a given steady state
but also to recognize that state during differentiation. Regulation of these organizational forces appears to be under the
control of chemical factors, and a hormonal concept of regulation has evolved. Hormones have been considered to act by reacting
with a specific target site. This may be part of their mode of action, but I would like to suggest that a hormone enters and
becomes part of a total molecular resonance system. In so doing, the entire molecular system of the cell is modified.
Of the known plant hormones, the cytokinins, because of their role in experimentally induced cell division and differentiation,
serve as a probe of hormonal involvement in differentiation. Cultured somatic cells of tobacco plants can be induced to undergo
differentiation by addition of cytokinin and auxin to the medium. Studies of the cytokinin hormones show a series of diverse
molecular involvements. The archetype cytokinin, N6-(Δ2-isopentenyl) adenosine (i6Ado), occurs in some molecular species of tRNA where it plays a vital role in the codon-anticodon interaction of tRNA and
m-RNA. i6Ado under-goes extensive metabolism in the tobacco tissue. It is either degraded to adenosine or converted to derivatives
that possess biological activity. It is perhaps, therefore, more correct to consider the hormone function as being derived
from this total metabolic web.
The normal somatic cells of tobacco cultures spontaneously change occasionally into an autonomous form that requires no external
growth factors. This line of cells synthesizes i6Ado. The metabolic web of the hormone-dependent strain can be perturbed by added auxin but such is not the case in the autonomous
strain. These data provide some insight into the altered state of cytokinin activity in which a cell line changes into an
autonomous form. Curiously, in become independent of the requirement for exogenous cytokinin, the autonomous tissue becomes
sensitive to added cytokinin. i6Ado also inhibits the growth of lines of mammalian cancer cells grown in culture.
Presented in the formal symposium on Information Transfer in Eukaryotic Cells, at the 26th Annual Meeting of the Tissue Culture
Association, Montreal, Quebec, June 2–5, 1975. 相似文献
16.
"植物组织培养"实践操作技能培养与提高 总被引:3,自引:0,他引:3
通过运用多媒体辅助教学、规范的演示实验、指导学生善于观察和总结等教学手段,为“植物组织培养”实践操作技能的培养打好基础,同时,重点让学生亲自动手操作实验,通过技能性实践、验证性实践、探索性实践,培养和提高《植物组织培养》实践操作技能. 相似文献
17.
Development of a helical-ribbon impeller bioreactor for high-density plant cell suspension culture 总被引:2,自引:0,他引:2
Jolicoeur M Chavarie C Carreau PJ Archambault J 《Biotechnology and bioengineering》1992,39(5):511-521
A double helical-ribbon impeller (HRI) bioreactor with a 11-L working volume was developed to grow high-density Catharanthus roseus cell suspensions. The rheological behavior of this suspension was found to be shear-thinning for concentrations higher than 12 to 15 g DW . L(-1). A granulated agar suspension of similar rheological properties was used as a model fluid for these suspensions. Mixing studies revealed that surface baffling and bottom profiling of the bioreactor and impeller speeds of 60 to 150 rpm ensured uniform mixing of suspensions. The HRI power requirement was found to increase singnificantly for agar suspensions higher than 13 g DW . L(-1), in conjunction with the effective viscosity increase. Oxygen transfer studies showed high apparent surface oxygen transfer coefficients (k(L)a approximately 4 to 45 h(-1)) from agar suspensions of 30 g DW . L(-1) to water and for mixing speeds ranging from 120 to 150 rpm. These high surface k(I)a values were ascribed to the flow pattern of this bioreactor configuration combined with surface bubble generation and entrainment in the liquid phase caused by the presence of the surface baffles. High-density C. roseus cell suspension cultures were successfully grown in this bioreactor without gas sparging. Up to 70% oxygen enrichment of the head space was required to ensure sufficient oxygen supply to the cultures so that dissolved oxygen concentration would remain above the critical level (>/=10% air saturation). The best mixing speed was 120 rpm. These cultures grew at the same rate ( approximately 0.4 d(-1)) and attained the same high biomass concentrations ( approximately 25 to 27 g DW . L(-1), 450 to 500 g filtered wet biomass . L(-1), and 92% to 100% settled wet biomass volume) as shake flask cultures. The scale-up potential of this bioreactor configuration is discussed. 相似文献
18.
Effect of medium acidity on growth and rooting of different plant species growing in vitro 总被引:2,自引:0,他引:2
C. Leifert Samantha Pryce P. J. Lumsden W. M. Waites 《Plant Cell, Tissue and Organ Culture》1992,30(3):171-179
Micropropagated Choisya, Daphne, Delphinium, Hemerocallis, Hosta, Iris and Photinia were found to adjust the pH of Murashige and Skoog's plant tissue culture medium (initial pH 5.6 or 3.5) to different values depending on the species. When plant growth and rooting rates were determined after plants had been grown on media initially adjusted or buffered to values between 2.6 and 5.7 the different plant species were also found to have distinct pH requirements for optimal growth and/or rooting rates.Abbreviations MS
Murashige & Skoog's (1962) medium
- MS19
MS with additionally 10 g l–1 sucrose
- 80 mg l–1
adenine sulphate and 130.9 mg l–1 NaH2PO4
- BA
6-benzyladenine
- NAA
1-naphthyl-acetic acid
- IBA
3-indole-butyric acid
- IAA
3-indole-acetic acid
- 2iP
N6(2-isopentyl) adenine 相似文献
19.
Protocorm-like bodies (PLBs) formed on leaf segmentsin vitro were used as explants for bioreactor cultures. Continuous immersion cultures (air lift column and air lift-balloon bioreactor),
and temporary immersion cultures (with or without charcoal filter attached) were used for the culture of PLB sections. A temporary
immersion culture with charcoal filter attached was most suitable for PLB culture. About 18,000 PLBs were harvested from 20
g of inoculum (∼1000 PLB sections) in 2 l Hyponex medium after 8 weeks of incubation. Aeration in a bioreactor at 0.5 or 2.0
volume of air per volume of medium min−1 (vvm) yielded similar levels of biomass production. PLBs grown in bioreactors were cultured on solid Murashige and Skoog,
Vacin and Went, Knudson C, Lindemann and Hyponex media. Hyponex medium was found to be suitable for conversion of PLBs into
plantlets and 83% of PLBs transformed into plantlets on this medium. The feasibility of using PLBs for large-scale micropropagation
was evaluated for scaled-up liquid cultures in bioreactors, rate of proliferation, and regeneration.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
20.
Regeneration of plants using nutrient mist culture 总被引:2,自引:0,他引:2
Pamela J. Weathers Kenneth L. Giles 《In vitro cellular & developmental biology. Plant》1988,24(7):727-732
Summary A nutrient mist was used forin vitro culture of plant tissue in a novel bioreactor, wherein the tissues were grown on a biologically inert screen within a sterile
chamber which allows excess media to drain away from the tissue. Plants tested includedDaucus, Lycopersicon, Ficus, Cinchona, andBrassica. The latter 4 genera were fully regenerated within the bioreactor. Tissue inocula included callus, anthers, and shoot meristems.
All plants grew at least as well in nutrient mists as in agar and always produced a greater quantity of shoots of a higher
quality and often faster than agar cultures. Cost analysis estimates showed up to a 65% savings in production costs (labor
and materials) could be realized using nutrient mist culture. Nutrient mist culture offers significant improvements in the
micropropagation of plants. 相似文献