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1.
James D. Kelly Phillip N. Miklas 《Molecular breeding : new strategies in plant improvement》1998,4(1):1-11
Diseases are regarded as the leading constraint to increased common bean (Phaseolus vulgaris L.) production worldwide. The range in variability and complexity among bean pathogens can be controlled with different single gene and quantitative resistance sources. Combining these resistance sources into commercial cultivars is a major challenge for bean breeders. To assist breeders, a major effort to identify RAPD markers tightly linked to different genes was undertaken. To date, 23 RAPD and five SCAR markers linked to 15 different resistance genes have been identified, in addition to QTL conditioning resistance to seven major pathogens of common bean. We review the feasibility of using marker-assisted selection (MAS) to incorporate disease resistance into common bean. Indirect selection of single resistance genes in the absence of the pathogen and the opportunity afforded breeders to pyramid these genes to improve their longevity and retain valuable hypostatic genes is discussed. The role of markers linked to the QTL controlling complex resistance and the potential to combine resistance sources using marker based selection is reviewed. Improving levels of selection efficiency using flanking markers, repulsion-phase linkages, co-dominant marker pairs, recombination-facilitated MAS and SCAR markers is demonstrated. Marker-assisted selection for disease resistance in common bean provides opportunities to breeders that were not feasible with traditional breeding methods. 相似文献
2.
Mukhlesur Rahman Genyi Li Dana Schroeder Peter B. E. McVetty 《Molecular breeding : new strategies in plant improvement》2010,26(3):439-453
Seed coat color inheritance in Brassica napus was studied in F1, F2, F3 and backcross progenies from crosses of five black seeded varieties/lines to three pure breeding yellow seeded lines. Maternal
inheritance was observed for seed coat color in B. napus, but a pollen effect was also found when yellow seeded lines were used as the female parent. Seed coat color segregated from
black to dark brown, light brown, dark yellow, light yellow, and yellow. Seed coat color was found to be controlled by three
genes, the first two genes were responsible for black/brown seed coat color and the third gene was responsible for dark/light
yellow seed coat color in B. napus. All three seed coat color alleles were dominant over yellow color alleles at all three loci. Sequence related amplified polymorphism
(SRAP) was used for the development of molecular markers co-segregating with the seed coat color genes. A SRAP marker (SA12BG18388) tightly linked to one of the black/brown seed coat color genes was identified in the F2 and backcross populations. This marker was found to be anchored on linkage group A9/N9 of the A-genome of B. napus. This SRAP marker was converted into sequence-characterized amplification region (SCAR) markers using chromosome-walking technology.
A second SRAP marker (SA7BG29245), very close to another black/brown seed coat color gene, was identified from a high density
genetic map developed in our laboratory using primer walking from an anchoring marker. The marker was located on linkage group
C3/N13 of the C-genome of B. napus. This marker also co-segregated with the black/brown seed coat color gene in B. rapa. Based on the sequence information of the flanking sequences, 24 single nucleotide polymorphisms (SNPs) were identified between
the yellow seeded and black/brown seeded lines. SNP detection and genotyping clearly differentiated the black/brown seeded
plants from dark/light/yellow-seeded plants and also differentiated between homozygous (Y2Y2) and heterozygous (Y2y2) black/brown
seeded plants. A total of 768 SRAP primer pair combinations were screened in dark/light yellow seed coat color plants and
a close marker (DC1GA27197) linked to the dark/light yellow seed coat color gene was developed. These three markers linked
to the three different yellow seed coat color genes in B. napus can be used to screen for yellow seeded lines in canola/rapeseed breeding programs. 相似文献
3.
R. O. Nodari S. M. Tsail R. L. Gilbertson P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,85(5):513-520
Summary A restriction fragment length polymorphism (RFLP)-based linkage map for common bean (Phaseolus vulgaris L.) covering 827 centiMorgans (cM) was developed based on a F2 mapping population derived from a cross between BAT93 and Jalo EEP558. The parental genotypes were chosen because they exhibited differences in evolutionary origin, allozymes, phaseolin type, and for several agronomic traits. The segregation of 152 markers was analyzed, including 115 RFLP loci, 7 isozyme loci, 8 random amplified polymorphic DNA (RAPD) marker loci, and 19 loci corresponding to 15 clones of known genes, 1 virus resistance gene, 1 flower color gene, and 1 seed color pattern gene. Using MAPMAKER and LINKAGE-1, we were able to assign 143 markers to 15 linkage groups, whereas 9 markers remained unassigned. The average interval between markers was 6.5 cM; only one interval was larger than 30 cM. A small fraction (9%) of the markers deviated significantly from the expected Mendelian ratios (121 or 31) and mapped into four clusters. Probes of known genes belonged to three categories: seed proteins, pathogen response genes, and Rhizobium response genes. Within each category, sequences homologous to the various probes were unlinked. The I gene for bean common mosaic virus resistance is the first disease resistance gene to be located on the common bean genetic linkage map. 相似文献
4.
M. Erfatpour A. Navabi K. P. Pauls 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(6):1331-1343
Key message
A QTL for non-darkening seed coat from ‘Wit-rood boontje’ was mapped in pinto bean population on chromosome Pv10, comprising 40 candidate genes.Abstract
The seed coat colour darkens with age in some market classes of dry beans (Phaseolus vulgaris), including pinto bean. Beans with darkened seed coats are discounted in the market place, since they are believed to be associated with lower nutritional quality, increased cooking time, and decreased palatability. The objective of this research was to map a non-darkening gene from a cranberry-like bean ‘Wit-rood boontje’ using a recombinant inbred line population, derived from a cross between ’Wit-rood boontje’ and a slow-darkening pinto bean (1533-15). The population was characterized for seed phenotype and genotyped with an Illumina BeadChip. A genetic linkage map was constructed with 1327 informative SNP markers plus an STS marker (OL4S500) and an SSR marker (Pvsd-0028), previously associated with the J gene and Sd gene, respectively, as well as non-darkening and slow-darkening phenotypes. The linkage map spanned 1253.2 cM over 11 chromosomes. A major QTL for the non-darkening trait was flanked by SNP 715646341 and SNP 715646348 on chromosome Pv10. The region, which spanned 13.2 cM, explained 48% of the phenotypic variation for seed coat darkening. Forty candidate genes were identified in the QTL interval. This information can be used to develop a gene-based marker to facilitate breeding non-darkening pinto beans and may lead to a better understanding of the molecular mechanism for the postharvest darkening phenomenon in pinto bean.5.
SCAR,RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean 总被引:3,自引:0,他引:3
A. F. Adam-Blondon M. Sévignac H. Bannerot M. Dron 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,88(6-7):865-870
Anthracnose, caused by the fungusColletotrichum lindemuthianum, is a severe disease of common bean (Phaseolus vulgaris L.) controlled, in Europe, by a single dominant gene,Are. Four pairs of near-isogenic lines (NILs) were constructed, in which theAre gene was introgressed into different genetic backgrounds. These pairs of NILs were used to search for DNA markers linked to the resistance gene. Nine molecular markers, five RAPDs and four RFLPs, were found to discriminate between the resistant and the susceptible members of these NILs. A backcross progeny of 120 individuals was analysed to map these markers in relation to theAre locus. Five out of the nine markers were shown to be linked to theAre gene within a distance of 12.0 cM. The most tightly linked, a RAPD marker, was used to generate a pair of primers that specifically amplify this RAPD (sequence characterized amplified region, SCAR).The research was supported by the CNRS and the Ministère Français de l'Education Nationale 相似文献
6.
A seed and flower color marker (P), nine seed protein, nine isozyme and 224 restriction fragment length polymorphism marker loci were used to construct a linkage map of the common bean, Phaseolus vulgaris L. (n = 11). The mapping population consisted of a backcross progeny between the Mesoamerican breeding line 'XR-235-1-1' and the Andean cultivar 'Calima'; the former was used as the recurrent parent. A bean PstI genomic library enriched for single copy sequences (95%) was the source of DNA probes. Sixty percent of the probes tested detected polymorphisms between the parental genotypes with at least one of the four restriction enzymes used here (DraI, EcoRI, EcoRV and HindIII). The computer software Mapmaker was used to determine the linkage relationships and linear order of segregating markers. These markers assorted into 11 linkage groups covering 960 cM of the bean genome. Partial linkage data were used to estimate the total length of the genome at 1200 cM. This estimate and that for the physical size of the genome yield an average ratio of 530 kb/cM. The relatively small size of the genome makes this crop species a good candidate for the isolation of genes via chromosome walking techniques. 相似文献
7.
Generation and mapping of SCAR and CAPS markers linked to the seed coat color gene in Brassica napus using a genome-walking technique. 总被引:2,自引:0,他引:2
Shushu Xiao Jinsong Xu Yuan Li Lei Zhang Shijun Shi Shuwen Shi Jiangsheng Wu Kede Liu 《Génome》2007,50(7):611-618
The yellow seed coat trait in No. 2127-17, a resynthesized purely yellow Brassica napus line, is controlled by a single partially dominant gene, Y. A double-haploid population derived from the F1 of No. 2127-17 x 'ZY821' was used to map the seed coat color phenotype. A combination of AFLP analysis and bulked segregant analysis identified 18 AFLP markers linked to the seed coat color trait. The 18 AFLP markers were mapped to a chromosomal region of 37.0 cM with an average of 2.0 cM between adjacent markers. Two markers, AFLP-K and AFLP-H, bracketed the Y locus in an interval of 1.0 cM, such that each was 0.5 cM away from the Y locus. Two other markers, AFLP-A and AFLP-B, co-segregated with the seed color gene. For ease of use in breeding programs, these 4 most tightly linked AFLP markers were converted into reliable PCR-based markers. SCAR-K, which was derived from AFLP-K, was assigned to linkage group 9 (N9) of a B. napus reference map consisting of 150 commonly used SSR (simple sequence repeat) markers. Furthermore, 2 SSR markers (Na14-E08 and Na10-B07) linked to SCAR-K on the reference map were reversely mapped to the linkage map constructed in this study, and also showed linkage to the Y locus. These linked markers would be useful for the transfer of the dominant allele Y from No. 2127-17 to elite cultivars using a marker-assisted selection strategy and would accelerate the cloning of the seed coat color gene. 相似文献
8.
Utility of RAPD markers in identifying genetic linkages to genes of economic interest in peach 总被引:7,自引:0,他引:7
M. L. Warburton V. L. Becerra-Velásquez J. C. Goffreda F. A. Bliss 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(5-6):920-925
The identification of molecular markers linked to economically important traits for use in crop improvement is very important in long-lived perennial species. Three-hundred-and-sixty RAPD primers were used with bulked segregant analysis to identify markers linked to loci of specific interest in peach [(Prunus persica) L. Batch] and peach x almond [(Prunus dulcis) Batch] crosses. The traits analyzed included flesh color, adhesion, and texture; pollen fertility; plant stature; and three isozyme loci. The Mendelian behavior of the RAPD loci was established, and RAPD markers were mapped relative to the loci controlling flesh color, adhesion, and texture, and the isozyme loci Mdh-1, 6Pgd-2 and Aat-1, as well as the existing RFLP genetic linkage map constructed previously using a peach x almond F2 population. This technique has facilitated rapid identification of RAPD and RFLP markers that are linked to the traits under study. Loci controlling these traits mapped predominantly to linkage groups 2 and 3 of the peach genetic linkage map. Linkages to genes with both dominant and co-dominant alleles were identified, but linkages to dominant genes were more difficult to find. In several crosses, RAPD marker bands proved to be allelic. One co-dominant RAPD formed a heteroduplex band in heterozygous individuals and in mixtures of alternate homozygotes. The Mendelian behavior of the RAPD loci studied was established and the results suggest that RAPD markers will be useful for plant improvement in peach. 相似文献
9.
S. He Z.-H. Yu C. E. Vallejos S. A. Mackenzie 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,90(7-8):1056-1062
The Fr gene in common bean, Phaseolus vulgaris L., is a unique gene for the study of plant nuclear-mitochondrial interactions because it appears to directly influence plant mitochondrial genome structure, resulting in the restoration of pollen fertility in cytoplasmic male sterile plants. This gene action is distinct from other pollen fertility restoration systems characterized to date. As a first step towards the map-based cloning of this unusual nuclear gene, we identified RAPD markers linked to Fr using bulked segregant analysis of near-isogenic lines. Using DNA gel blot hybridization, we localized the identified RAPD markers to a linkage group on the common bean RFLP map and constructed a linkage map of the Fr region using both RAPD markers and RFLP markers. Analysis of the mode of Fr action with the aid of identified Fr-linked DNA markers indicated that Fr functions in a semidominant fashion, showing dosage effect in controlling the dynamics of a heteroplasmic mitochondrial population. We also present our observations on the developmental distinctions, crucial in the accurate mapping of the Fr gene, between spontaneous cytoplasmic reversion and Fr-driven fertility restoration, two phenomena that are phenotypically indistinguishable. 相似文献
10.
Cobos MJ Fernández MJ Rubio J Kharrat M Moreno MT Gil J Millán T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(7):1347-1353
Two recombinant inbred line (RIL) populations derived from intraspecific crosses with a common parental line (JG62) were employed to develop a chickpea genetic map. Molecular markers, flower colour, double podding, seed coat thickness and resistance to fusarium wilt race 0 (FOC-0) were included in the study. Joint segregation analysis involved a total of 160 markers and 159 RILs. Ten linkage groups (LGs) were obtained that included morphological markers and 134 molecular markers (3 ISSRs, 13 STMSs and 118 RAPDs). Flower colour (B/b) and seed coat thickness (Tt/tt) appeared to be linked to STMS (GAA47). The single-/double-podding locus was located on LG9 jointly with two RAPD markers and STMS TA80. LG3 included a gene for resistance to FOC-0 (Foc01/foc01) flanked by RAPD marker OPJ20600 and STMS marker TR59. The association of this LG with FOC-0 resistance was confirmed by QTL analysis in the CA2139 × JG62 RIL population where two genes were involved in the resistance reaction. The STMS markers enabled comparison of LGs with preceding maps. 相似文献
11.
R. Freyre P. W. Skroch V. Geffroy A.-F. Adam-Blondon A. Shirmohamadali W. C. Johnson V. Llaca R. O. Nodari P. A. Pereira S.-M. Tsai J. Tohme M. Dron J. Nienhuis C. E. Vallejos P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):847-856
Three RFLP maps, as well as several RAPD maps have been developed in common bean (Phaseolus vulgaris L.). In order to align these maps, a core linkage map was established in the recombinant inbred population BAT93×Jalo EEP558
(BJ). This map has a total length of 1226 cM and comprises 563 markers, including some 120 RFLP and 430 RAPD markers, in addition
to a few isozyme and phenotypic marker loci. Among the RFLPs mapped were markers from the University of California, Davis
(established in the F2 of the BJ cross), University of Paris-Orsay, and University of Florida maps. These shared markers allowed us to establish
a correspondence between the linkage groups of these three RFLP linkage maps. In total, the general map location (i.e., the
linkage group membership and approximate location within linkage groups) has been determined for some 1070 markers. Approaches
to align this core map with other current or future maps are discussed.
Received: 10 March 1998 / Accepted: 22 April 1998 相似文献
12.
Elena Pérez-Vega Astrid Pañeda Cristina Rodríguez-Suárez Ana Campa Ramón Giraldez Juan José Ferreira 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(7):1367-1380
The objective of this research was to determine the quantitative trait loci (QTLs) controlling phenological traits (days to
flowering, days to end of flowering, days to harvest as green pod, and days to maturity), seed size traits (seed length, seed
height, seed width, and seed weight), and seed quality traits (water absorption, and coat proportion), in common bean. A population
of 104 F7 recombinant inbred lines (RILs) derived from an inter-gene pool cross between Xana, and Cornell 49242, was used to develop
a genetic linkage map including 175 AFLPs, 27 microsatellites, 30 SCARs, 33 ISSRs, 12 RAPDs, 13 loci codifying for seed proteins,
and the four genes Fin,fin (growth habit); Asp,asp (seed coat shininess); P,p (seed color); and I,i (resistance to bean common mosaic virus). The map has a total length of 1,042 cM distributed across 11 linkage groups aligned
to those of the core linkage map of bean using common molecular markers as anchor points. The QTL analyses were carried out
over three environments using the mean environment data with composite interval mapping. Thirty-one QTLs for ten traits were
found to be significant in at least one environment and in the mean environment data, the number of significant QTLs identified
per trait ranging from two to five. Twenty-seven of these QTLs mapped forming clusters in eight different chromosomal regions.
The rationale for this clustered mapping and the possible relationship between some QTLs for phenological traits and the genes
Fin and I are discussed. 相似文献
13.
Amine Zraidi Gertraud Stift Martin Pachner Abdolali Shojaeiyan Li Gong Tamas Lelley 《Molecular breeding : new strategies in plant improvement》2007,20(4):375-388
Using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), simple sequence repeats (SSR),
and morphological traits, the first genetic maps for Cucurbita pepo (2n=2x=40) were constructed and compared. The two mapping populations consisted of 92 F2 individuals each. One map was developed from a cross between an oil-seed pumpkin breeding line and a zucchini accession,
into which genes for resistance to Zucchini Yellow Mosaic Virus (ZYMV) from a related species, C. moschata, had been introgressed. The other map was developed from a cross between an oil-seed pumpkin and a crookneck variety. A total
of 332 and 323 markers were mapped in the two populations. Markers were distributed in each map over 21 linkage groups and
covered an average of 2,200 cM of the C. pepo genome. The two maps had 62 loci in common, which enabled identification of 14 homologous linkage groups. Polyacrylamide
gel analyses allowed detection of a high number of markers suitable for mapping, 10% of which were co-dominant RAPD loci.
In the Pumpkin-Zucchini population, bulked segregant analysis (BSA) identified seven markers less than 7 cM distant from the
locus n, affecting lignification of the seed coat. One of these markers, linked to the recessive hull-less allele (AW11-420), was
also found in the Pumpkin-Crookneck population, 4 cM from n. In the Pumpkin-Zucchini population, 24 RAPD markers, previously introduced into C. pepo from C. moschata, were mapped in two linkage groups (13 and 11 markers in LGpz1 and LGpz2, respectively), together with two sequence characterized
amplified region (SCAR) markers linked to genes for resistance to ZYMV. 相似文献
14.
Nedim Mutlu Phillip N. Miklas Dermot P. Coyne 《Molecular breeding : new strategies in plant improvement》2006,17(2):127-135
Resistance (R) genes containing nucleotide-binding site (NBS)-leucine rich repeats (LRR) are the most prevalent types of R
gene in plants. The objective of this study was to develop PCR-based R-gene analog polymorphism (RGAP) markers for common
bean (Phaseolus vulgaris L). Twenty degenerate primers were designed from the conserved kinase-1a (GVGKTT) and hydrophobic domains (GLPLAL) of known
NBS-LRR type R-genes and from EST databases. Sixty-six of the 100 primer combinations tested yielded polymorphism. Thirty-two
RGAP markers were mapped in the BAT 93/Jalo EEP558 core mapping population for common bean. The markers mapped to 10 of 11
linkage groups with a strong tendency for clustering. In addition, the RGAP markers co-located, on six linkage groups, with
15 resistance gene analogs (RGAs) that were previously mapped in other populations of common bean. The distance between the
priming sites in NBS-LRR type R-genes is around 500 bp. Of the 32 RGAP markers, 19 had sizes larger and 13 less than 500 bp.
RGAP markers mapped close to known R-genes on B11, and to QTLs for resistance on B1, B2, B6, B7, B8, B10, and B11. RGAP appears
to provide a useful marker technique for tagging and mapping R-genes in segregating common bean populations, discovery of
candidate genes underlying resistance QTL, and future cloning of R-genes in common bean. 相似文献
15.
In common bean (Phaseolus vulgaris L.), the expression of color in flower and seedcoat tissues requires the dominant allele of the P gene. The fully recessive p allele completely suppresses color expression in these tissues, whereas in specific genetic backgrounds, the p(gri) allele potentiates a grayish-white seedcoat and pale violet (nearly white) flowers with two violet dots on the banner petals. As a first step to gaining a better understanding of this important gene, we phenotypically scored an F2 population segregating for P and p(gri) and subsequently screened contrasting bulk DNA samples with oligonucleotide primers to uncover random amplified polymorphic DNA (RAPD) fragments. OU3(2300), an RAPD marker linked in coupling phase to the dominant allele, mapped 1.3 cM from P. The core 'BAT93' x 'Jalo EEP558' recombinant inbred population was scored, and the marker mapped to linkage group B7. The segregating fragment was cloned, sequenced, and shown to possess significant homology to the Ty3-gypsy class of retrotransposons. We have named the element Tpv3g. It is estimated that about 100 copies of the element are present in the common bean genome. Phylogenetic analysis placed Tpv3g in the class A group of plant retrotransposons. 相似文献
16.
Alzate-Marin Menarim G. Soares Baía Paula JR K. Almeida De Souza Da Costa E. Gonçalves De Barros & Moreira 《Journal of Phytopathology》2001,149(5):259-264
The inheritance of anthracnose resistance of the common bean ( Phaseolus vulgaris L.) differential cultivar G 2333 to Colletotrichum lindemuthianum races 73 and 89 was studied in crosses with the susceptible cultivar Rudá. The segregation ratios of 15 : 1 in the F2 and 3 : 1 in the backcrosses to Rudá indicate that for each of the races tested there are two independent resistance loci in G 2333. A random amplified polymorphic DNA (RAPD) molecular marker (OPH181200C ) linked in resistance to race 73 was identified in a BC3 F2:3 population derived from crosses between Rudá and G 2333. A RAPD molecular marker OPAS13950C , previously identified as linked to gene Co-42 , was also amplified in this population. Co-segregation analyses showed that these two markers are located at 5.6 (OPH181200C ) and 11.2 (OPAS13950C ) cM of the Co-42 gene. These markers were not present in BC1 F2:3 plants resistant to race 89 indicating that this population carries a different resistance gene. DNA amplification of BC1 F2:3 plants with RAPD molecular marker OPAB450C , previously identified as linked to gene Co-5 , indicated that this gene is present in this population. 相似文献
17.
Blair MW Iriarte G Beebe S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1149-1163
Advanced backcross QTL analysis was used to identify quantitative trait loci (QTL) for agronomic performance in a population
of BC2F3:5 introgression lines created from the cross of a Colombian large red-seeded commercial cultivar, ICA Cerinza, and a wild common
bean accession, G24404. A total of 157 lines were evaluated for phenological traits, plant architecture, seed weight, yield
and yield components in replicated trials in three environments in Colombia and genotyped with microsatellite, SCAR, and phaseolin
markers that were used to create a genetic map that covered all 11 linkage groups of the common bean genome with markers spaced
at an average distance of every 10.4 cM. Segregation distortion was most significant in regions orthologous for a seed coat
color locus (R-C) on linkage group b08 and two domestication syndrome genes, one on linkage group b01 at the determinacy (fin) locus and the other on linkage group b02 at the seed-shattering (st) locus. Composite interval mapping analysis identified a total of 41 significant QTL for the eight traits measured of which
five for seed weight, two for days to flowering, and one for yield were consistent across two or more environments. QTL were
located on every linkage group with b06 showing the greatest number of independent loci. A total of 13 QTL for plant height,
yield and yield components along with a single QTL for seed size showed positive alleles from the wild parent while the remaining
QTL showed positive alleles from the cultivated parent. Some QTL co-localized with regions that had previously been described
to be important for these traits. Compensation was observed between greater pod and seed production and smaller seed size
and may have resulted from QTL for these traits being linked or pleiotropic. Although wild beans have been used before to
transfer biotic stress resistance traits, this study is the first to attempt to simultaneously obtain a higher yield potential
from wild beans and to analyze this trait with single-copy markers. The wild accession was notable for being from a unique
center of diversity and for contributing positive alleles for yield and other traits to the introgression lines showing the
potential that advanced backcrossing has in common bean improvement. 相似文献
18.
Matthew W. Blair Juliana I. Medina Carolina Astudillo Judith Rengifo Steve E. Beebe Gloria Machado Robin Graham 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(6):1059-1070
Iron and zinc deficiencies are human health problems found throughout the world and biofortification is a plant breeding-based
strategy to improve the staple crops that could address these dietary constraints. Common bean is an important legume crop
with two major genepools that has been the focus of genetic improvement for seed micronutrient levels. The objective of this
study was to evaluate the inheritance of seed iron and zinc concentrations and contents in an intra-genepool Mesoamerican × Mesoamerican
recombinant inbred line population grown over three sites in Colombia and to identify quantitative trait loci (QTL) for each
mineral. The population had 110 lines and was derived from a high-seed iron and zinc climbing bean genotype (G14519) crossed
with a low-mineral Carioca-type, prostrate bush bean genotype (G4825). The genetic map for QTL analysis was created from SSR
and RAPD markers covering all 11 chromosomes of the common bean genome. A set of across-site, overlapping iron and zinc QTL
was discovered on linkage group b06 suggesting a possibly pleiotropic locus and common physiology for mineral uptake or loading.
Other QTL for mineral concentration or content were found on linkage groups b02, b03, b04, b07, b08 and b11 and together with
the b06 cluster were mostly novel compared to loci found in previous studies of the Andean genepool or inter-genepool crosses.
The discovery of an important new locus for seed iron and zinc concentrations may facilitate crop improvement and biofortification
using the high-mineral genotype especially within the Mesoamerican genepool. 相似文献
19.
Pedrosa A Vallejos CE Bachmair A Schweizer D 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(2):205-212
Fluorescent in situ hybridisation of pooled, closely linked RFLP markers was used to integrate the genetic linkage map and the mitotic chromosome map of the common bean. Pooled RFLP probes showed clear and reproducible signals and allowed the assignment of all linkage groups to the chromosomes of two Phaseolus vulgaris cultivars, Saxa and Calima. Low extension values for signals originating from clustered RFLPs suggest that these clones are physically close to each other and that clusters in the genetic map are not a result of suppression of recombination due to the occurrence of chromosome rearrangements. For linkage group K, clustering of markers could be associated with proximity to centromeres. High variation in the number of 45S rDNA loci was observed among cultivars, suggesting that these terminal sites are highly recombinogenic in common bean. 相似文献
20.
Tatiana de Campos Paula Rodrigues Oblessuc Danilo Augusto Sfor?a Juliana Morini Kupper Cardoso Renata Moro Baroni Adna Cristina Barbosa de Sousa S��rgio Augusto Morais Carbonell Alisson Fernando Chioratto Antonio Augusto Franco Garcia Luciana Benchimol Rubiano Anete Pereira de Souza 《Molecular breeding : new strategies in plant improvement》2011,27(4):549-560
The genetic linkage map for the common bean (Phaseolus vulgaris L.) is a valuable tool for breeding programs. Breeders provide new cultivars that meet the requirements of farmers and consumers, such as seed color, seed size, maturity, and growth habit. A genetic study was conducted to examine the genetics behind certain qualitative traits. Growth habit is usually described as a recessive trait inherited by a single gene, and there is no consensus about the position of the locus. The aim of this study was to develop a new genetic linkage map using genic and genomic microsatellite markers and three morphological traits: growth habit, flower color, and pod tip shape. A mapping population consisting of 380 recombinant F10 lines was generated from IAC-UNA × CAL143. A total of 871 microsatellites were screened for polymorphisms among the parents, and a linkage map was obtained with 198 mapped microsatellites. The total map length was 1865.9 cM, and the average distance between markers was 9.4 cM. Flower color and pod tip shape were mapped and segregated at Mendelian ratios, as expected. The segregation ratio and linkage data analyses indicated that the determinacy growth habit was inherited as two independent and dominant genes, and a genetic model is proposed for this trait. 相似文献