Some Additional Artifacts From The Fanning Site (14DP1)

Abstract

A report of the contents of eleven cache pits found at the Fanning site.     

The isolation and partial characterization of ribonuclease A from Bison bison

1. Bison ribonuclease was isolated from pancreas glands of Bison bison by acid extraction, (NH(4))(2)SO(4) fractionation, affinity chromatography on Sepharose-5'-(4-aminophenylphosphoryl)uridine 2',3'-phosphate and ion-exchange chromatography on Bio-Rex-70. 2. The selectivity of the affinity column towards bison ribonuclease in heterogeneous protein solutions was greatly improved by employing piperazine buffers at pH5.3, which decreased non-specific interactions of other proteins. Rapid desorption from the affinity column was obtained with sodium phosphate buffer (pH3). 3. Bison ribonuclease has a total amino acid content very similar to ox ribonuclease. Inactivation of bison ribonuclease with iodoacetic acid leads to the formation of 0.62 residues of pi-carboxymethylhistidine and 0.36 residues of tau-carboxymethylhistidine. The amino acid composition of peptides isolated from diagonal peptide ;maps' and also of peptides isolated after pH1.6 and 2.4 two-dimensional high-voltage electrophoresis of a digest of bison ribonuclease labelled with pyridoxal 5-phosphate indicates that there is complete homology between ox and bison ribonucleases. 4. The Schiff-base attachment site of pyridoxal 5-phosphate was identified as lysine-41 by NaBH(4) reduction followed by peptide isolation.     

延边安图立新发现的砾石石器

2006年9月,在延边地区安图县永庆乡立新村东北的第4级阶地上发现石制品71件;其中,6件出自粉砂质黄土层,其余为地表采集.石制品的种类包括石核、石片、细石叶、工具和断块等,石制品体现出砾石工业的特征,遗址的年代属于旧石器时代晚期或稍早.     

Non-Bison Remains from the Vore Bison Jump,Crook County,Wyoming

Abstract

The Vore. Bison Jump is a multi-component Late-Preh1stonc B1son kill site in the Black Hills of Crook County, Wyoming. Examination of the non Bison revealed a large number of carnivores, mainly wolves. Analysis of these wolf remains showed a need for more careful identification of canid remains from archaeological sites in light of incipient domestication of wolves at this site.