Jackson County, Illinois, U.S.A., Photo by Nathan Soley (right) Champaign County, Illinois, U.S.A., Photo by Nathan Soley (left) Soley and Sipes “Reproductive biology and pollinators of the invasive shrub Autumn olive (Elaeagnus umbellata Thunberg)”. https://doi.org/10.1111/1442-1984.12307
Left panel: Lateral view of a lenticular cell of Valonia utricularis at the beginning (upper) and the end (lower) of a 22-hr observation. Arrowheads with numbers indicate carbon particles along the cell outline. Right panel: (upper) Changes in cell outline and position of the numbered carbon particles in the lenticular cell at 2-hr intervals. (lower) The segmental extension profile of the lenticular cell showing the anisotropic cell surface growth. Numbers represent those used for labelling the carbon particles. See Mine et al. in this issue for details.
Beautiful underwater view of an edible brown alga, Sargassum fusiforme (Fucales, Phaeophyceae) at the rocky shore in Sakurajima, Kagoshima, Japan (Photo by Ryuta Terada). Characteristic responses of the PSII photochemical efficiency on desiccation and salinity gradients in S. fusiforme are reported by Yonemori et al. in this issue.
A tidal pool dinoflagellate, Chiharadinium hexapraecingulum (T. Horiguchi & Chihara) Dawut & T. Horiguchi gen. & comb. nov. Upper row: Light micrographs of lateral view, ventral view, epifluorescence micrograph of motile cells and scanning electron micrograph of apical view of a cell. Lower row: Scanning electron micrographs of ventral view, left lateral view and antapical view of motile cells. Establishment of a new genus Chiharadinium is reported by Dawut et al. in this issue.
A 3D printed stereotaxic head mount that enables high resolution imaging of the anterior chamber of the eye in alert and freely mobile mice is designed in this study. The system is placed non‐invasively using temporal mount bars and a snout mount, without breaking the skin or risking suffocation, while an endoscopic channel stabilizes the ocular probes. Further details can be found in the article by Bjorn Paulson, Sangwook Lee, Miyeon Jue, Kyungsung Lee, Sanghwa Lee, Guk Bae Kim, Youngjin Moon, Joo Yong Lee, Namkug Kim, and Jun Ki Kim ( e201960188 ).
A false‐colored and merged image of fresh, ex vivo rat kidney acquired using an excitation‐scanning hyperspectral imaging system. The spectral image was acquired using excitation wavelengths from 360 to 550 nm. Colors represent principal components extracted from a spectral image cube featuring no added labels or markers. Further details can be found in the article by Peter F. Favreau, Joshua A. Deal, Bradley Harris, et al. ( e201900183 ).
Leptocereus santamarinae. In Herradura beach in Las Tunas Province. Photo by Duniel Barrios. Flores et al. https://esj-journals.onlinelibrary.wiley.com/doi/abs/10.1111/1442-1984.12334 (right) Echinocereus enneacanthus (Cactaceae). Spring of 2020, Mapimi Biosphere Reserve, Southern Chihuahuan Desert, Durango, Mexico. Photo by José Antonio Aranda-Pineda. Aranda-Pineda et al. https://esj-journals.onlinelibrary.wiley.com/doi/abs/10.1111/1442-1984.12329 (left)
A type of compact and cost‐effective light‐sheet imaging device, termed sub‐voxel‐resolving light‐sheet add‐on module (SLAM), is developed to cooperate with conventional 2D epifluorescence microscope, allowing high‐contrast, resolution‐improved 3D imaging of various biological samples at high throughput. Further details can be found in the article by Fang Zhao, Yicong Yang, Yi Li, et al. ( e201960243 ).
Cover Image Cyrtopodium paludicolum. This photo was taken by Dr. João C. F. Cardoso, on the Panga Ecological Station, Uberlândia City, Minas-Gerais state, Brazil.
The family of vibrational spectroscopic imaging techniques grows every few years and there is a need to compare and contrast new modalities with the better understood ones. Three vibrational spectroscopy techniques (High Definition FT‐IR, Raman and AFM‐IR) were applied for subcellular chemical imaging of cholesteryl esters in PC‐3 prostate cancer cells. The techniques were compared and contrasted in terms of image quality, spectral pattern, and chemical information. Further details can be found in the article by Maciej Roman, Tomasz P. Wrobel, Czeslawa Paluszkiewicz, and Wojciech M. Kwiatek ( e201960094 ).
Probing amyloid formation of intrinsically disordered proteins (IDPs) in aqueous solutions with ionic liquids (ILs) could help identify the IL–IDP interactions causing the process in this media. Takekiyo et al. report that ethylammonium nitrate (EAN) initiates the formation of intermolecular β-sheet structure – amyloid-like aggregates – of α-synuclein. The cations and anions of EAN function as charges to mask side chains, leading to the formation of a polyproline II helix that assists the formation of α-synuclein aggregates. The present results could help develop promoting agents for amyloid aggregates in biomaterials science. (DOI: 10.1002/bip.23352)
Scanning electron micrographs of two members of the marine diatom genus Nagumoea, N. africana (the left four photos) and N. serrata (the right two). This genus is characterized as unique by the ladder-like structure of its internal valve as shown in the image. Morphology and molecular phylogeny of this genus are reported by Sugawara et al. in this issue.
The detection of colon cancer by using Poincaré sphere and 2D polarimetric imaging approach. Further details can be found in the article by Deyan Ivanov, Viktor Dremin, Alexander Bykov, Ekaterina Borisova, et al. ( e202000082 ).
Photographs of Kulikovskiyia triundulata gen. et sp. nov. along with holotypic locality. Upper left: Photograph of the holotypic locality (lateritic rocky plateau of Maharashtra, India). Upper middle: Live cell showing chloroplast structure in valve view. Upper right: DIC image of a frustule in valve view. Lower: SEM image of the external valve showing peripheral and apical spines, siliceous slats and tri‐undulate valve shape. See Roy et al. in this issue. Cover picture from: Article link here
A novel 0.9 mm-diameter intravascular photoacoustic catheter with coaxial excitation and detection was developed to overcome the limitation of imaging range. A miniature ring-shaped ultrasound transducer with a 0.18 mm-diameter orifice in the center was successfully fabricated. The results demonstrated that the coaxial catheter exhibited much better photoacoustic/ultrasound imaging performance from the intima to the adventitia. Further details can be found in the article by Riqiang Lin, Qi Zhang, Shengmiao Lv, Jiaming Zhang, Xiatian Wang, Dongliang Shi, Xiaojing Gong, and Kwok-ho La ( e202200269 ).
Confocal Raman microscopy provides composition and constitution of label-free samples at high spatial-resolution. In this study, the histology of atherosclerotic arteries was measured using a custom-built confocal Raman microscopy that was developed to improve imaging speed, diffraction efficiency, and spectral resolution. Additionally, the machine learning method was used to enhance the accuracy of classification. Results show that each layer of arteries and lipid-rich inflamed plaque can be successfully characterized. Further details can be found in the article by Jingchao Xing, Dong-Ryoung Lee, Jin Won Kim, and Hongki Yoo ( e202200243 ).
