共查询到20条相似文献,搜索用时 31 毫秒
1.
Wenjuan Yao Xiaozhao Deng Hui Zhong Miao Liu Pu Zheng Zhihao Sun Yun Zhang 《Journal of industrial microbiology & biotechnology》2009,36(7):911-921
Corynebacterium glutamicum strains are used for the fermentative production of l-glutamate. Five C. glutamicum deletion mutants were isolated by two rounds of selection for homologous recombination and identified by Southern blot analysis.
The growth, glucose consumption and glutamate production of the mutants were analyzed and compared with the wild-type ATCC
13032 strain. Double disruption of dtsR1 (encoding a subunit of acetyl-CoA carboxylase complex) and pyc (encoding pyruvate carboxylase) caused efficient overproduction of l-glutamate in C. glutamicum; production was much higher than that of the wild-type strain and ΔdtsR1 strain under glutamate-inducing conditions. In the absence of any inducing conditions, the amount of glutamate produced by
the double-deletion strain ΔdtsR1Δpyc was more than that of the mutant ΔdtsR1. The activity of phosphoenolpyruvate carboxylase (PEPC) was found to be higher in the ΔdtsR1Δpyc strain than in the ΔdtsR1 strain and the wild-type strain. Therefore, PEPC appears to be an important anaplerotic enzyme for glutamate synthesis in
ΔdtsR1 derivatives. Moreover, this conclusion was confirmed by overexpression of ppc and pyc in the two double-deletion strains (ΔdtsR1Δppc and ΔdtsR1Δpyc), respectively. Based on the data generated in this investigation, we suggest a new method that will improve glutamate production
strains and provide a better understanding of the interaction(s) between the anaplerotic pathway and fatty acid synthesis. 相似文献
2.
Förster A Jacobs K Juretzek T Mauersberger S Barth G 《Applied microbiology and biotechnology》2007,77(4):861-869
The yeast Yarrowia lipolytica secretes high amounts of various organic acids, like citric (CA) and isocitric (ICA) acids, triggered by growth limitation
caused by different factors and an excess of carbon source. Depending on the carbon source used, Y. lipolytica strains produce a mixture of CA and ICA in a characteristic ratio. To examine whether the CA/ICA product ratio can be influenced
by gene-dose-dependent overexpression or by disruption of the isocitrate lyase (ICL)-encoding gene ICL1, recombinant Y. lipolytica strains were constructed, which harbour multiple ICL1 copies or a defective icl1 allele. The high-level expression of ICL in ICL1 multicopy integrative transformants resulted in a strong shift of the CA/ICA ratio into direction of CA. On glycerol, glucose
and sucrose, the ICA proportion decreased from 10–12% to 3–6%, on sunflower oil or hexadecane even from 37–45% to 4–7% without
influencing the total amount of acids (CA and ICA) produced. In contrast, the loss of ICL activity in icl1-defective strains resulted in a moderate 2–5% increase in the ICA proportion compared to ICL wild-type strains on glucose
or glycerol. 相似文献
3.
Aner Gurvitz 《Molecular genetics and genomics : MGG》2009,282(4):407-416
Mycobacterium tuberculosis represents a severe threat to human health worldwide. Therefore, it is important to expand our knowledge of vital mycobacterial
processes, such as that effected by fatty acid synthase type 2 (FASII), as well as to uncover novel ones. Mycobacterial FASII
undertakes mycolic acid biosynthesis, which relies on a set of essential enzymes, including 3-oxoacyl-AcpM reductase FabG1/Rv1483.
However, the M. tuberculosis genome encodes four additional FabG homologs, designated FabG2–FabG5, whose functions have hitherto not been characterized
in detail. Of the four candidates, FabG4/Rv0242c was recently shown to be essential for the survival of M. bovis BCG. The present work was initiated by assessing the suitability of yeast oar1Δ mutant cells lacking mitochondrial 3-oxoacyl-ACP reductase activity to act as a surrogate system for expressing FabG1/MabA
directed to the mitochondria. Mutant yeast cells producing this targeted FabG1 variant were essentially wild type for all
of the chronicled phenotype characteristics, including respiratory growth on glycerol medium, cytochrome assembly and lipoid
acid production. This indicated that within the framework of de novo fatty acid biosynthesis in yeast mitochondria, FabG1
was able to act on shorter (C4) acyl substrates than was previously proposed (C8–20) during mycolic acid biosynthesis in M. tuberculosis. Thereafter, FabG2–FabG5 were expressed as mitochondrial proteins in the oar1Δ strain, and FabG4 was found to complement the mutant phenotype and contain high levels of 3-oxoacyl-thioester reductase
activity. Hence, like FabG1, FabG4 is also an essential, physiologically functional 3-oxoacyl-thioester reductase, albeit
the latter’s involvement in mycobacterial FASII remains to be explored. 相似文献
4.
To disclose the addition of some strong promotional amino acids (namely glycine, glutamate, lysine and aspartic acid) is how
to improve the glycerol productivity of Candida glycerinogenes. An amino acid addition strategy based on dynamic enzyme activity was applied to improve glycerol productivity and decrease
the byproducts formation in a fermentation of C. glycerinogenes in a 7-1 bioreactor. Compared with the control, after feeding glycine, glutamate, lysine and aspartic acid, glycerol productivity
obtained an increase of 22.3, 25.6, 23.5 and 28.6%, respectively; meanwhile, the amounts of ethanol, acetic acid and pyruvate
decreased largely. Whichever glycine, lysine, glutamate or aspartic acid was fed could elevate the activities of glucose-6-phosphate
dehydrogenase (G6PDH), citrate synthase (CIT), triosephosphate isomerase (TPI) and cytoplasmic NAD+AEAAKw-dependent glycerol-3-phosphate
dehydrogenase (ctGPD), and reduce the activities of pyruvate kinase (PYK), phosphofructokinase (PFK) and alcohol dehydrogenase
(ADH). The reason of glycerol overproduction by the yeasts after feeding glycine, glutamate, lysine or aspartic acid is that
the anaplerosis of intermediate metabolites in TCA cycle for amino acid degradation can decrease the flux from Embden-Meyerhof-Parnas
(EMP) pathway to TCA cycle and enhance the flux through glycerol biosynthesis pathway. Above all, not only the high active
hexose monophosphate (HMP) pathway but also the high dihydroxyacetone phosphate (DHAP) level plays an important role in the
high glycerol productivity of C. glycerinogenes. The strategy of amino acid supplement is significant and can be economically implemented by an online process control strategy
for higher yield of glycerol in industrial scale.
Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2009, Vol. 45, No. 3, pp. 338–343.
The article is published in the original. 相似文献
5.
6.
Production of <Emphasis Type="SmallCaps">l</Emphasis>-alanine by metabolically engineered <Emphasis Type="Italic">Escherichia coli</Emphasis> 总被引:2,自引:0,他引:2
Zhang X Jantama K Moore JC Shanmugam KT Ingram LO 《Applied microbiology and biotechnology》2007,77(2):355-366
Escherichia coli W was genetically engineered to produce l-alanine as the primary fermentation product from sugars by replacing the native d-lactate dehydrogenase of E. coli SZ194 with alanine dehydrogenase from Geobacillus stearothermophilus. As a result, the heterologous alanine dehydrogenase gene was integrated under the regulation of the native d-lactate dehydrogenase (ldhA) promoter. This homologous promoter is growth-regulated and provides high levels of expression during anaerobic fermentation.
Strain XZ111 accumulated alanine as the primary product during glucose fermentation. The methylglyoxal synthase gene (mgsA) was deleted to eliminate low levels of lactate and improve growth, and the catabolic alanine racemase gene (dadX) was deleted to minimize conversion of l-alanine to d-alanine. In these strains, reduced nicotinamide adenine dinucleotide oxidation during alanine biosynthesis is obligately
linked to adenosine triphosphate production and cell growth. This linkage provided a basis for metabolic evolution where selection
for improvements in growth coselected for increased glycolytic flux and alanine production. The resulting strain, XZ132, produced
1,279 mmol alanine from 120 g l−1 glucose within 48 h during batch fermentation in the mineral salts medium. The alanine yield was 95% on a weight basis (g
g−1 glucose) with a chiral purity greater than 99.5% l-alanine.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
7.
A novel Candida glycerinogenes mutant, which possesses high glycerol productivity in a high phosphate concentration medium, was obtained by mutagenesis of an industrial glycerol producer. The mutant accumulated a total biomass of 11.5 g l−1, which is less than the 15 g l−1of the wild-type strain, but it consumed glucose faster than the wild-type strain did. The mutant reached its maximal glycerol concentration of 129 g l−1 in 84 h compared to 96 h for the wild-type strain. High cytoplasmic glycerol-3-phosphate dehydrogenase activity of the mutant in the early glycerol formation phase, leading to a rapid glycerol synthesis and accumulation, may be the main reason for the short fermentation process. 相似文献
8.
Morita T Konishi M Fukuoka T Imura T Kitamoto D 《Applied microbiology and biotechnology》2007,74(2):307-315
Vegetable oil is the usual carbon source for the production of biosurfactants (BS), mannosylerythritol lipids (MEL). To simplify
the procedures of BS production and recovery, we investigated the extracellular production of MEL from water-soluble carbon
sources instead of vegetable oils by using two representative yeast strains. The formation of extracellular MEL from glucose
was confirmed by thin layer chromatography (TLC) and HPLC analysis. On glucose cultivation, pure MEL were easily prepared
by only solvent extraction of the culture medium, different from the case of soybean oil cultivation. The fatty acid profile
of the major MEL produced from glucose was similar to that produced from soybean oil based on GC–MS analysis. The resting
cells of Pseudozyma antarctica T-34 produced MEL by feeding of glucose only and gave a yield of 12 g l−1. In contrast, Pseudozyma aphidis ATCC 32657 gave no MEL from glucose. Moreover, the extracellular lipase activities were detected at high levels during the
cultivation regardless of the carbon sources. These results indicate that all the biosynthesis pathways for MEL in P. antarctica T-34 should constitutively function. In conclusion, P. antarctica T-34 thus has potential for BS production from glucose. 相似文献
9.
Jing Yu Jiaxi Jiang Zian Fang Yuyang Li Hong Lv Jianping Liu 《Biotechnology letters》2010,32(4):507-512
Inulinase gene (Kcinu) derived from Kluyveromyces cicerisporus was expressed extracellularly in Kluyveromyces lactis using an episomal vector directed by Kcinu promoter. The influence of hap1 gene disruption on the expression of inulinase was studied. Inulinase activity in the supernatant of the recombinant Klhap1Δ strain was 391 U ml−1 after cultured 120 h, which was 2.2-fold that of the wild type host. The relative inulinase mRNA level of the Klhap1Δ strain was 11.3-fold that of the wild type strain, and the expression plasmid was more stable in the mutant host. Based on
these results, the disruption of hap1 facilitated the high and stable expression of inulinase controlled by Kcinu promoter in K. lactis. 相似文献
10.
Okino S Suda M Fujikura K Inui M Yukawa H 《Applied microbiology and biotechnology》2008,78(3):449-454
In mineral salts medium under oxygen deprivation, Corynebacterium glutamicum exhibits high productivity of l-lactic acid accompanied with succinic and acetic acids. In taking advantage of this elevated productivity, C. glutamicum was genetically modified to produce d-lactic acid. The modification involved expression of fermentative d-lactate dehydrogenase (d-LDH)-encoding genes from Escherichia coli and Lactobacillus delbrueckii in l-lactate dehydrogenase (l-LDH)-encoding ldhA-null C. glutamicum mutants to yield strains C. glutamicum ΔldhA/pCRB201 and C. glutamicum ΔldhA/pCRB204, respectively. The productivity of C. glutamicum ΔldhA/pCRB204 was fivefold higher than that of C. glutamicum ΔldhA/pCRB201. By using C. glutamicum ΔldhA/pCRB204 cells packed to a high density in mineral salts medium, up to 1,336 mM (120 g l−1) of d-lactic acid of greater than 99.9% optical purity was produced within 30 h. 相似文献
11.
Cheng Zhang Hong Zong Bin Zhuge Xinyao Lu Huiying Fang Jiali Zhu Jian Zhuge 《Biotechnology and Bioprocess Engineering》2016,21(1):95-102
The regeneration of Candida glycerinogenes protoplasts is a major step following genetic manipulations such as fusion and DNA-mediated transformation. An investigation of protoplast formation and cytological examination was used to gain further insight into the loss of protoplast viability in osmotically stabilized support media. Protoplasts with the highest regeneration frequency (98.6% protoplasts/mL) were isolated, using lysozyme dissolved in 1M sorbitol osmoticum. The commercial enzyme preparations, osmotic stabilisers, and growth phase were effective in raising the protoplast yield. Sodium chloride was effective for protoplast preparation; however, sugars and sugar alcohols were better for protoplast regeneration. Sorbitol at a concentration of 1 M was used in regeneration agar for further studies. Regeneration of colonies from protoplasts was maximal (11 ~ 15%) when protoplasts were incorporated in cooled agar containing 0.5% glucose, supplemented with 1M sorbitol as osmotic stabilizer. C. glycerinogenes strain was highly sensitive to zeocin, so transformation of protoplasts and PEG-mediated was achieved with an improved transformation system, using plasmid pURGAP-gfp containing zeocin gene driven by a PCgGAP promoter from C. glycerinogenes to express gfp gene and be transformed into the 5.8S rDNA site of C. glycerinogenes in order to test the system for studying the yeast osmoregulation. We developed an efficient method for transformation of C. glycerinogenes, and parameters involved in transformation efficiency were optimized. Expressions of gfp at different levels were conducted under osmotic stress containing NaCl, KCl, sorbitol or glycerol for the recombinant strains. These improved procedures for protoplast isolation, regeneration and transformation proved to be useful applications in genetic studies for other Candida species and industrial yeast. 相似文献
12.
Huiling Wu Jiachang Zhang Liusheng Duan A. Egrinya Eneji Bo Zhang Zhaohu Li 《World journal of microbiology & biotechnology》2011,27(2):325-331
Coronatine (COR) is a structural and functional analogue of jasmonic acid that might be employed in agriculture to elicit
plant resistance against various aggressors. However, the yield of COR is low both in chemosynthesis and biosynthesis, so
broad investigation of COR is difficult. Coronatine combines two distinct components: coronafacic acid (CFA) and coronamic
acid (CMA). Synthesis of both CMA and CFA is involved in l-isoleucine metabolism, so the objective of this work was to investigate if COR production can be improved by regulating amino
acid biosynthesis in P. syringae pv. glycinea. Inhibition of dihydrodipicolinate synthase was achieved by removing the dapA gene via homologous recombination, which resulted in a COR yield by the dapA
− mutant of about 1.5-fold greater than the wild strain. Thus, regulation of amino acid metabolism is a feasible way to increase
COR production, which could be a more effective method than adding substrates into culture medium. 相似文献
13.
V. N. Verbenko L. V. Kuznetsova E. P. Krupyan A. V. Suslov 《Russian Journal of Genetics》2009,45(8):917-923
Plasmid pUC19-recAoc carrying a mutant allele of the recA gene, which plays the key role in the control of the SOS repair system and homologous recombinational repair, causes a 1.5-fold
increase in radiation resistance of Escherichia coli ΔrecA cells, as compared to the wild-type recA
+ cells. The protective effect of this plasmid is drastically reduced in mutant lexA3 recAΔ21 deficient in the LexA protein and in induction of the SOS regulon. Plasmid pUC19-recAoc effectively suppresses UV sensitivity of the ΔrecA mutant. Mutation recAo20 allows constitutive high-level synthesis of the RecA protein. This mutation impairs the SOS box in the operator site of the
recA gene and enhances heterology of the dimer LexA binding site. These data confirm that high level of the RecA protein synthesis
per se is not sufficient for the expression of γ-inducible functions and that the derepression of lexA-dependent genes, other than recA gene, is necessary for the complete induction of the SOS repair system. 相似文献
14.
Methee Khamduang Kanoktip Packdibamrung Jarun Chutmanop Yusuf Chisti Penjit Srinophakun 《Journal of industrial microbiology & biotechnology》2009,36(10):1267-1274
The production of l-phenylalanine is conventionally carried out by fermentations that use glucose or sucrose as the carbon source. This work
reports on the use of glycerol as an inexpensive and abundant sole carbon source for producing l-phenylalanine using the genetically modified bacterium Escherichia coli BL21(DE3). Fermentations were carried out at 37°C, pH 7.4, using a defined medium in a stirred tank bioreactor at various
intensities of impeller agitation speeds (300–500 rpm corresponding to 0.97–1.62 m s−1 impeller tip speed) and aeration rates (2–8 L min−1, or 1–4 vvm). This highly aerobic fermentation required a good supply of oxygen, but intense agitation (impeller tip speed
~1.62 m s−1) reduced the biomass and l-phenylalanine productivity, possibly because of shear sensitivity of the recombinant bacterium. Production of l-phenylalanine was apparently strongly associated with growth. Under the best operating conditions (1.30 m s−1 impeller tip speed, 4 vvm aeration rate), the yield of l-phenylalanine on glycerol was 0.58 g g−1, or more than twice the best yield attainable on sucrose (0.25 g g−1). In the best case, the peak concentration of l-phenylalanine was 5.6 g L−1, or comparable to values attained in batch fermentations that use glucose or sucrose. The use of glycerol for the commercial
production of l-phenylalanine with E. coli BL21(DE3) has the potential to substantially reduce the cost of production compared to sucrose- and glucose-based fermentations. 相似文献
15.
Kenro Tokuhiro Nobuhiro Ishida Eiji Nagamori Satoshi Saitoh Toru Onishi Akihiko Kondo Haruo Takahashi 《Applied microbiology and biotechnology》2009,82(5):883-890
Expression of a heterologous l-lactate dehydrogenase (l-ldh) gene enables production of optically pure l-lactate by yeast Saccharomyces cerevisiae. However, the lactate yields with engineered yeasts are lower than those in the case of lactic acid bacteria because there
is a strong tendency for ethanol to be competitively produced from pyruvate. To decrease the ethanol production and increase
the lactate yield, inactivation of the genes that are involved in ethanol production from pyruvate is necessary. We conducted
double disruption of the pyruvate decarboxylase 1 (PDC1) and alcohol dehydrogenase 1 (ADH1) genes in a S. cerevisiae strain by replacing them with the bovine l-ldh gene. The lactate yield was increased in the pdc1/adh1 double mutant compared with that in the single pdc1 mutant. The specific growth rate of the double mutant was decreased on glucose but not affected on ethanol or acetate compared
with in the control strain. The aeration rate had a strong influence on the production rate and yield of lactate in this strain.
The highest lactate yield of 0.75 g lactate produced per gram of glucose consumed was achieved at a lower aeration rate. 相似文献
16.
Bracesco N Candreva EC Keszenman D Sánchez AG Soria S Dell M Siede W Nunes E 《Radiation and environmental biophysics》2007,46(4):401-407
Checkpoints are components of signalling pathways involved in genome stability. We analysed the putative dual functions of
Rad17 and Chk1 as checkpoints and in DNA repair using mutant strains of Saccharomyces cerevisiae. Logarithmic populations of the diploid checkpoint-deficient mutants, chk1Δ/chk1Δ and rad17Δ/rad17Δ, and an isogenic wild-type strain were exposed to the radiomimetic agent bleomycin (BLM). DNA double-strand breaks (DSBs)
determined by pulsed-field electrophoresis, surviving fractions, and proliferation kinetics were measured immediately after
treatments or after incubation in nutrient medium in the presence or absence of cycloheximide (CHX). The DSBs induced by BLM
were reduced in the wild-type strain as a function of incubation time after treatment, with chromosomal repair inhibited by
CHX. rad17Δ/rad17Δ cells exposed to low BLM concentrations showed no DSB repair, low survival, and CHX had no effect. Conversely, rad17Δ/rad17Δ cells exposed to high BLM concentrations showed DSB repair inhibited by CHX. chk1Δ/chk1Δ cells showed DSB repair, and CHX had no effect; these cells displayed the lowest survival following high BLM concentrations.
Present results indicate that Rad17 is essential for inducible DSB repair after low BLM-concentrations (low levels of oxidative
damage). The observations in the chk1Δ/chk1Δ mutant strain suggest that constitutive nonhomologous end-joining is involved in the repair of BLM-induced DSBs. The differential
expression of DNA repair and survival in checkpoint mutants as compared to wild-type cells suggests the presence of a regulatory
switch-network that controls and channels DSB repair to alternative pathways, depending on the magnitude of the DNA damage
and genetic background.
Nelson Bracesco and Ema C. Candreva have contributed equally to this article. 相似文献
17.
Plant vacuolar Na+/H+ antiporter plays an important role in salt tolerance. A vacuolar Na+/H+ antiporter gene TrNHX1 was cloned from Trifolium repens L., a forage legume, by RT-PCR and RACE methods using degenerate oligonucleotide primers. The TrNHX1 sequence contains 2,394 nucleotides and an open-reading frame of 1,626 nucleotides that encodes a protein of 541 amino acids
with a deduced molecular mass of 59.5 kDa. The deduced amino acid sequence of TrNHX1 is 78% identical to that of a vacuolar
Na+/H+ antiporter of Arabidopsis thaliana, AtNHX1, and contains the consensus amiloride-binding domain. TrNHX1 could partially complement the NaCl-sensitive phenotypes
of yeast mutants Δnhx1 and Δena1-4Δnhx1, and a similar complementation was also observed in the presence of LiCl and KCl. In addition, it was found that TrNHX1 suppressed
the hygromycin-sensitive phenotype of yeast mutant Δena1-4Δnhx1. The expression of TrNHX1 in T. repens increased in the presence of 150 mM NaCl, and this result accords with that of Na+ contents determination under the same treatment. These results suggest that TrNHX1 functions as a vacuolar Na+/H+ antiporter and plays an important role in salt tolerance and ion homeostasis in T. repens. 相似文献
18.
Sun QL Wang LY Shan JJ Jiang R Guo LH Zhang Y Zhang R Li Y 《Archives of microbiology》2007,188(4):333-340
Streptomyces sp. 139 produces a novel exopolysaccharide (EPS) designated Ebosin which has antagonistic activity for IL-1R in vitro and
remarkable anti-rheumatic arthritis activity in vivo. We previously identified a ste (Streptomyces eps) gene cluster consisting of 27 ORFs responsible for Ebosin biosynthesis. The gene product of ste15 shows high homology to known glycosyltransferases (GTFs). To elucidate its function in Ebosin biosynthesis, the ste15 gene was knocked out with a double crossover via homologous recombination. Our analysis of monosaccharide composition for
EPS-m produced by the mutant strain Streptomyces sp. 139 (ste15
−) showed that glucose was significantly diminished compared to its natural counterpart Ebosin. This derivative of Ebosin lost
the antagonistic activity for IL-1R in vitro and its molecular mass was smaller than Ebosin. These results have demonstrated
that the ste15 gene codes for a GTF for glucose, which is functionally involved in Ebosin biosynthesis. 相似文献
19.
Saccharomyces cerevisiae grows very poorly in dilute acid lignocellulosic hydrolyzate during the anaerobic fermentation for fuel ethanol production.
However, yeast cells grown aerobically on the hydrolyzate have increased tolerance for the hydrolyzate. Cultivation of yeast
on part of the hydrolyzate has therefore the potential of enabling increased ethanol productivity in the fermentation of the
hydrolyzate. To evaluate the ability of the yeast to grow in the hydrolyzate, fed-batch cultivations were run using the ethanol
concentration as input variable to control the feed-rate. The yeast then grew in an undetoxified hydrolyzate with a specific
growth rate of 0.19 h−1 by controlling the ethanol concentration at a low level during the cultivation. However, the biomass yield was lower for
the cultivation on hydrolyzate compared to synthetic media: with an ethanol set-point of 0.25 g/l the yield was 0.46 g/g on
the hydrolyzate, compared to 0.52 g/g for synthetic media. The main reason for the difference was not the ethanol production per se, but a significant production of glycerol at a high specific growth rate. The glycerol production may be attributed to an
insufficient respiratory capacity. 相似文献
20.
M. V. Padkina L. V. Parfenova A. E. Gradoboeva E. V. Sambuk 《Applied Biochemistry and Microbiology》2010,46(4):409-414
The HuIFNA16, HuIFNB1, and BoIFNG genes encoding human α16, β-interferons and bovine γ-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed.
There was no effect of high level of heterologous protein synthesis on the yeast P. pastoris cell growth, unlike yeast Saccharomyces cerevisiae. The considerable part of the heterologous interferons was detected in the yeast P. pastoris soluble protein fraction but not in the “inclusion bodies.” The treatment of human β-interferon with endoglycosidase H showed
that protein was expressed in glycosylated and unglycosylated forms. On the strength of these data, the hypothesis was suggested
that the more effective heterologous gene expression in yeast P. pastoris and enhanced resistance of the methylotrophic yeast to negative effects of recombinant proteins was due to the special features
of its metabolism. 相似文献