共查询到20条相似文献,搜索用时 0 毫秒
1.
2.
3.
4.
5.
Specificity factor of yeast mitochondrial RNA polymerase. Purification and interaction with core RNA polymerase 总被引:17,自引:0,他引:17
A H Schinkel M J Koerkamp E P Touw H F Tabak 《The Journal of biological chemistry》1987,262(26):12785-12791
We have identified a mitochondrial protein from Saccharomyces cerevisiae which confers the ability to recognize mitochondrial promoters onto a nonspecifically transcribing mitochondrial core RNA polymerase and we have purified this specificity factor 10,700-fold from a whole cell extract. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified fraction followed by elution and renaturation of protein activity shows that the specificity factor is a 43-kDa polypeptide which directs mitochondrial core RNA polymerase to promoters belonging to rRNA-, tRNA-, and protein-encoding genes, as well as to mitochondrial replication origins. Gel filtration and glycerol gradient sedimentation studies indicate that the specificity factor shows little association with core RNA polymerase in the absence of DNA, and that it behaves like a monomeric 43-kDa protein. 相似文献
6.
7.
A heteromeric transcription factor required for mammalian RNA polymerase II 总被引:6,自引:1,他引:5 下载免费PDF全文
S Kitajima Y Tanaka T Kawaguchi T Nagaoka S M Weissman Y Yasukochi 《Nucleic acids research》1990,18(16):4843-4849
8.
9.
10.
11.
Transcription specificity of Xenopus laevis RNA polymerase A 总被引:2,自引:0,他引:2
12.
13.
14.
15.
16.
17.
18.
19.
Characterization of the RNA binding properties of transcription factor IIIA of Xenopus laevis oocytes 总被引:2,自引:12,他引:2 下载免费PDF全文
P J Romaniuk 《Nucleic acids research》1985,13(14):5369-5387