血清HIF-1α、HSP27、HSP70、HSP90α与肝细胞癌患者经肝动脉化疗栓塞术治疗疗效的关系研究

摘要 目的：探讨血清缺氧诱导因子-1α（HIF-1α）、热休克蛋白（HSP）27、HSP70、HSP90α与肝细胞癌（HCC）患者经肝动脉化疗栓塞术（TACE）治疗疗效的关系。方法：选取2021年6月～2023年6月在南充市中心医院接受TACE治疗的HCC患者125例,根据TACE治疗疗效分为无效组（47例）和有效组（78例）。采用酶联免疫吸附法检测血清HIF-1α、HSP27、HSP70、HSP90α。多因素Logistic回归分析影响HCC患者TACE治疗疗效的因素,受试者工作特征（ROC）曲线分析血清HIF-1α、HSP27、HSP70、HSP90α对HCC患者TACE治疗无效的预测价值。结果：与有效组比较,无效组血清HIF-1α、HSP27、HSP70、HSP90α水平升高（P＜0.05）。多因素Logistic回归分析显示,低分化、血管侵犯和HIF-1α、HSP27、HSP70、HSP90α升高为HCC患者TACE治疗无效的独立危险因素（P＜0.05）。血清HIF-1α、HSP27、HSP70、HSP90α联合预测HCC患者TACE治疗无效的曲线下面积为0.958,大于血清HIF-1α、HSP27、HSP70、HSP90α单独预测的0.750、0.739、0.742、0.749。结论：血清HIF-1α、HSP27、HSP70、HSP90α升高是HCC患者TACE治疗无效的独立危险因素,血清HIF-1α、HSP27、HSP70、HSP90α联合预测HCC患者TACE治疗无效的价值较高。     

HSP86 and HSP84 exhibit cellular specificity of expression and co-precipitate with an HSP70 family member in the murine testis

This study extends to the protein level our previous observations, which had established the stage and cellular specificity of expression of hsp86 and hsp84 in the murine testis in the absence of exogenous stress. Immunoblot analysis was used to demonstrate that HSP86 protein was present throughout testicular development and that its levels increased with the appearance of differentiating germ cells. HSP86 was most abundant in the germ cell population and was present at significantly lower levels in the somatic cells. By contrast, the HSP84 protein was detected in the somatic cells of the testis rather than in germ cells. The steady-state levels of HSP86 and HSP84 paralleled the pattern of the expression of their respective mRNAs, suggesting that regulation at the level of translation was not a major mechanism controlling hsp90 gene expression in testicular cells. Immunoprecipitation analysis revealed that a 70-kDa protein coprecipitated with the HSP86/HSP84 proteins in testicular homogenates. This protein was identified as an HSP70 family member by immunoblot analysis, suggesting that HSP70 and HSP90 family members interact in testicular cells. © 1993Wiley-Liss, Inc.     

Inhibitors of the heat shock response: biology and pharmacology

A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity of this group of proteins for the treatment of a wide variety of human diseases. This review will describe the progress made to date in developing pharmacological modulators of the heat shock response, including both agents which affect the entire heat shock response and those that specifically target the HSP70 and HSP90 chaperone families.     

Cloning and differential expression of three heat shock protein genes associated with thermal stress from the wolf spider Pardosa pseudoannulata (Araneae: Lycosidae)

Pardosa pseudoannulata is the main predatory natural enemy of crop pests in a paddy ecosystem. When P. pseudoannulata is exposed to unfavorable temperature conditions, the response of heat shock proteins could resist the damage, and is therefore, conducive to the organism’s rapid adaptation to the surrounding stress environment. In this study, we explored the roles of hsp70 and hsp90 genes in response to heat stress, using the rapid amplification of cDNA ends technique and cloned full-length cDNAs of Pphsp70, Pphsp83, and Pphsp90. The mRNA expression levels of the three genes under different temperature stresses (25, 28, 31, 34, 37, 40, and 43 °C) and with different duration stresses (4, 8, 12, 16, and 20 h) were analyzed by quantitative real-time polymerase chain reaction. The full-length cDNA of Pphsp70, Pphsp83, and Pphsp90 was 2331 base pair (bp), 2466 bp, and 2663 bp, respectively. Phylogenetic analysis of amino acid sequences of Pphsp70, Pphsp83, and Pphsp90 showed that the sequences had high homology with that of other spiders. The mRNA expression of all three genes was extremely significantly up-regulated at 43 °C. Moreover at 43 °C, the expression of all three genes in both female and male spiders at the duration of 4 h was the highest compared to that of other stress duration groups. Therefore, it can be inferred that the three genes of P. pseudoannulata play a crucial protective role in resistance in a high-temperature environment.     

Moment closure and the stochastic logistic model

The quasi-stationary distribution of the stochastic logistic model is studied in the parameter region where its body is approximately normal. Improved asymptotic approximations of its first three cumulants are derived. It is shown that the same results can be derived with the aid of the moment closure method. This indicates that the moment closure method leads to expressions for the cumulants that are asymptotic approximations of the cumulants of the quasi-stationary distribution.     

A new look at the critical community size for childhood infections

Quasi-stationarity and time to extinction are studied for the classic endemic model. Attention is restricted to the transition region in parameter space where the quasi-stationary distribution is non-normal. A new approximation of the marginal distribution of infected individuals in quasi-stationarity is presented. It leads to a simple explicit expression for an approximation of the critical community size in terms of model parameters.     

Tissue-specific expression of heat shock proteins of the mouse in the absence of stress

The steady-state levels of four members of the heat shock proteins families (HSP84, HSC73, HSP71, and HSP25) were examined by immunoblot analysis of several different tissues of young and adult mice in the absence of stress. These hsps were detected in all tissues but their level was variable. The levels of HSC73 and HSP84 varied only slightly between different tissues in either young or adult mice, with the exception of skin where these hsps were found in reduced amounts. In contrast, the stress-inducible member of the HSP70 family, HSP71, was found to be expressed in all tissues but in amounts which differed by as much as two orders of magnitude between tissues. In general, the levels of both HSP71 and HSP25 were found to be tissue dependent, with higher levels found in tissues such as stomach, intestine, colon and bladder, tissues which are exposed to toxic environmental or metabolic products, and which may concentrate these substances by water resorption and/or be exposed to them for longer periods. The levels of HSP71 and HSP25 were generally positively correlated both in young and adult mice although this correlation was not found in certain tissues such as kidney, testes, and bone. Tissues of young mice contained lower amounts of HSP25 and HSP71 than were found in the same tissues from adults. We conclude that hsps are expressed in all tissues of the mouse in the absence of stress and that some organs, particularly those exposed to potentially toxic metabolites, show a higher level of expression of HSP71 and HSP25. © 1993Wiley-Liss, Inc.     

清胰利胆颗粒对重症急性胰腺炎患者血清HMGB1, HSP70, HSP27,IL-8水平的影响

目的:研究清胰利胆颗粒对重症急性胰腺炎患者血清高迁移率族蛋白1(HMGB1)、热休克蛋白70(HSP70)、热休克蛋白27(HSP27)、白介素-8(IL-8)水平的影响。方法:选取2015年8月至2016年7月我院收治的84例重症急性胰腺炎患者,根据随机数字法分为观察组和对照组,42例每组。对照组采取常规方案完成治疗,观察组在此基础上使用清胰利胆颗粒治疗。比较两组患者临床疗效,血淀粉酶恢复至正常时间、白细胞恢复至正常时间、胃肠功能恢复至正常时间、腹痛缓解时间及血清HMGB1、HSP70、HSP72、IL-8水平。结果:治疗后,观察组临床总有效率显著高于对照组[92.86%(39/42)比71.43%(30/42)](P0.05)。观察组的血淀粉酶恢复至正常时间、白细胞恢复至正常时间、胃肠功能恢复至正常时间及腹痛缓解时间显著短于对照组(P0.05)。治疗前,两组患者HMGB1、HSP70、HSP72、IL-8水平比较无显著差异(P0.05),治疗后,两组患者HMGB1、HSP70、HSP72、IL-8水平和治疗前相比显著下降(P0.05),和对照组相比,观察组的HMGB1、HSP70、HSP72、IL-8水平较低(P0.05)。结论:清胰利胆颗粒能有效降低重症急性胰腺炎患者血清HMGB1、HSP70、HSP27、IL-8水平,且可提高临床疗效。     

不同热休克蛋白在肾细胞癌组织中的表达及其意义

目的　研究HSPgp96、HSP90、HSP70在肾细胞癌组织中的表达及其相互关系。方法　应用免疫组化S P法检测 5 4例肾细胞癌组织标本中HSPgp96、HSP90、HSP70的表达情况。结果　在肾细胞癌组织中HSPgp96、HSP90、HSP70表达明显较正常肾组织增强 (P <0 0 5 ) ,阳性率分别为 88 9%、 85 2 %、 90 7% ,三者之间无明显差异性 (P >0 0 5 ) ,其表达程度均与肾细胞癌临床分期、病理分级及细胞类型无显著相关性 (P >0 0 5 )。结论　HSPgp96、HSP90、HSP70的高表达在肾细胞癌的发生、发展中起促进作用 ,尤其是在加强肿瘤抗原的呈递、表达上具有重要临床应用价值。