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Background  

Genome analyses have revealed that gene duplication in plants is rampant. Furthermore, many of the duplicated genes seem to have been created through ancient genome-wide duplication events. Recently, we have shown that gene loss is strikingly different for large- and small-scale duplication events and highly biased towards the functional class to which a gene belongs. Here, we study the expression divergence of genes that were created during large- and small-scale gene duplication events by means of microarray data and investigate both the influence of the origin (mode of duplication) and the function of the duplicated genes on expression divergence.  相似文献   

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An AP1/AGL9 group of MADS box gene, OMADS1, with extensive homology to the Arabidopsis AGAMOUS-like 6 gene (AGL6) was characterized from orchid (Oncidium Gower Ramsey). OMADS1 mRNA was detected in apical meristem and in the lip and carpel of flower. Yeast two-hybrid analysis indicated that OMADS1 is able to strongly interact with OMADS3, a TM6-like protein that was involved in flower formation and floral initiation in orchid. Transgenic Arabidopsis and tobacco ectopically expressed OMADS1 showed similar novel phenotypes by significantly reducing plant size, flowering extremely early, and losing inflorescence indeterminacy. In addition, homeotic conversion of sepals into carpel-like structures and petals into staminoid structures were also observed in flowers of 35S::OMADS1 Arabidopsis. This result indicated that OMADS1 was involved in floral formation and initiation in transgenic plants. Further analysis indicated that the expression of flowering time genes FT, SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1) and flower meristem identity genes LEAFY (LFY), APETALA1 (AP1) was significantly up-regulated in 35S::OMADS1 transgenic Arabidopsis plants. Furthermore, ectopic expression of OMADS1 rescued late-flowering phenotype in gi-1, co-3 but not for ft-1 and fwa-1 mutants. These results supported that ectopic expression of OMADS1 influenced flower transition and formation by acting as an activator for FT and SOC1 in Arabidopsis.  相似文献   

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To date, the effect of natural selection on candidate genes underlying complex traits has rarely been studied experimentally, especially under ecologically realistic conditions. Here we report that the effect of selection on the flowering time gene FRIGIDA (FRI) reverses depending on the season of germination and allelic variation at the interacting gene FLOWERING LOCUS C (FLC). In field studies of 136 European accessions of Arabidopsis thaliana, accessions with putatively functional FRI alleles had higher winter survival in one FLC background in a fall-germinating cohort, but accessions with deletion null FRI alleles had greater seed production in the other FLC background in a spring-germinating cohort. Consistent with FRI's role in flowering, selection analyses suggest that the difference in winter survival can be attributed to time to bolting. However, in the spring cohort, the fitness difference was associated with rosette size. Our analyses also reveal that controlling for population structure with estimates of inferred ancestry and a geographical restriction was essential for detecting fitness associations. Overall, our results suggest that the combined effects of seasonally varying selection and epistasis could explain the maintenance of variation at FRI and, more generally, may be important in the evolution of genes underlying complex traits.  相似文献   

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The gene FRIGIDA (FRI) is floral repressor and plays a key role in the timing of Arabidopsis flowering. To study the function of FRI-like genes in bamboo, we isolated a FRI family gene from bamboo Phyllostachys violascens and named it PvFRI-L. Sequence alignment and phylogenetic analysis show that the PvFRI-L protein belongs to the FRL3 (III) subfamily from monocots and contains a conserved FRIGIDA domain. PvFRI-L was located in the nucleus of onion epidermal cells. PvFRI-L was expressed in all tested organs of flowering and non-flowering bamboo plants with a higher expression in non-flowering than in flowering plants. Overexpression of PvFRI-L in Arabidopsis caused late flowering by downregulating flowering locus T and upregulating flowering locus C. A P-box, the binding site involved in gibberellin response, was found only in the promoter region of PvFRI-L but not in that of FRI. Furthermore, PvFRI-L expression in the leaves of Ph. violascens seedlings was downregulated with gibberellic acid treatment. Taking together, our observation suggests that PvFRI-L may be flowering repressor and its delaying floral timing may be regulated by gibberellic acid in bamboo.  相似文献   

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镉胁迫对拟南芥幼苗错配修复基因表达的影响   总被引:1,自引:0,他引:1  
采用半定量反转录聚合酶链式反应(RT-PCR)技术研究了Cd胁迫对拟南芥(Ara-bidopsis thaliana)幼苗错配修复和增殖细胞核抗原基因表达的影响,并结合幼苗的形态和生理指标,选取Cd胁迫敏感的生物标记物.结果表明:不同浓度(0.25、0.5、1.0 mg·L1)Cd处理对拟南芥幼苗叶片数、地上部鲜质量影响不大;Cd浓度为0.25 mg·L-1时,地上部分可溶性蛋白质含量明显升高(P<0.05),Cd浓度为0.5和1.0 mg·L-1时,可溶性蛋白质含量明显降低(P<0.05);叶绿素含量随着Cd浓度的增加而微弱增加(P>0.05).Cd浓度为0.25 mg·L-1时,以18S rRNA为内参照,PCNA1、PCNA2、MSH2、MSH3、MSH6、MSH7 6个基因均出现了诱导表达,当Cd浓度增加到1.0 mg·L-1时,除了MSH6持续表达诱导及MSH3基因与对照相比表达抑制外,其他基因的表达依然出现诱导,但都低于0.5 mg·L-1Cd处理下的基因表达水平.以上结果表明,基因表达的改变可作为检测Cd污染对植物遗传毒性效应潜在有用的生物标记物.  相似文献   

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Hagenblad J  Nordborg M 《Genetics》2002,161(1):289-298
Linkage disequilibrium in highly selfing organisms is expected to extend well beyond the scale of individual genes. The pattern of polymorphism in such species must thus be studied over a larger scale. We sequenced 14 short (0.5-1 kb) fragments from a 400-kb region surrounding the flowering time locus FRI in a sample of 20 accessions of Arabidopsis thaliana. The distribution of allele frequencies, as quantified by Tajima's D, varies considerably over the region and is incompatible with a standard neutral model. The region is characterized by extensive haplotype structure, with linkage disequilibrium decaying over 250 kb. In particular, recombination is evident within 35 kb of FRI in a haplotype associated with a functionally important allele. This suggests that A. thaliana may be highly suitable for linkage disequilibrium mapping.  相似文献   

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Virus-induced gene silencing (VIGS) has great potential as a reverse-genetics tool in plant genomics. In this study, we examined the potential of VIGS in soybean seeds and the emergence stage of soybean plants using Apple latent spherical virus (ALSV) vectors. Inoculation of an ALSV vector (soyPDS-ALSV) carrying a fragment of the soybean phytoene desaturase (soyPDS) gene into soybean seedlings resulted in a highly uniform photo-bleached phenotype, typical of PDS inhibition, on the upper leaves throughout plant growth. The photo-bleached phenotype was also found on all immature pods, all seed coats, and about 50% embryos of seeds on soybean plants infected with soyPDS-ALSV. Infection with an ALSV vector (soyIFS2-ALSV) having a fragment of soybean isoflavone synthase 2 (soyIFS2) gene also led to a reduction of the levels of both soyIFS2- and soyIFS1- mRNAs and an isoflavone content in the cotyledons of about 36% mature seeds of infected soybean plants. Furthermore, VIGS of soyPDS was induced in the next generation plants by the seed transmission of soyPDS-ALSV. Thus ALSV vectors will be useful for studying gene functions in the reproductive stages and early growth stages, such as emergence and cotyledon stages, in addition to the vegetative stages of soybean plants.  相似文献   

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We observed substantial variation in the time of flowering among 13 populations of Arabidopsis thaliana (Brassicaceae) from an extensive latitudinal range when grown under uniform experimental conditions. The later the onset of flowering, the greater was potential reproduction. Later flowering plants also had greater plasticity in a host of morphological and physiological traits measured in nutrient-rich vs. nutrient-poor test environments. This relationship between flowering time and overall plasticity was only apparent for traits measured at the time of seed production, not at the time of flowering or earlier. At the time of seed production in this short-lived annual, the regression of a multivariate measure of overall plasticity on the time of flowering was linear and highly significant (r2 = 0.90, P < 0.0001). These correlations among time of flowering, reproductive fitness, and plasticity support the idea that selection for late-flowering genotypes would select concomitantly for greater plasticity.  相似文献   

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By systematic sequencing of a flower bud cDNA library from Arabidopsis thaliana, we have identified four cDNAs encoding polygalacturonase. The corresponding genes, together with seven other A. thaliana genes present in the databases, form a small gene family. Sequence comparisons of the deduced polypeptides within the gene family or with other plant polygalacturonases allow classification of the genes into different clades. Five polygalacturonases, including all those isolated from the flower buds, are closely related to the enzyme in pollen. Of the six remaining polygalacturonases, three are more closely related to the abscission-specific type of enzyme and two others to the fruit polygalacturonase. The last one is more distantly related to the others and might correspond to a new type of polygalacturonase. Expression of the different genes was analysed on Northern blots and by a PCR-based strategy. Results indicate that if, as expected, the cDNAs isolated from the flower bud library are strongly expressed in pollen, other genes are expressed at a low level in young developing tissues, such as in seedlings and roots, suggesting that they could be implicated in the cell wall modifications observed during cell elongation and/or expansion which occur in these tissues.  相似文献   

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Liu  Yuan  Luo  Cong  Zhang  Xiu-Juan  Lu  Xin-Xi  Yu  Hai-Xia  Xie  Xiao-Jie  Fan  Zhi-Yi  Mo  Xiao  He  Xin-Hua 《Plant Cell, Tissue and Organ Culture》2020,143(1):219-228
Plant Cell, Tissue and Organ Culture (PCTOC) - CONSTANS (CO)/CONSTANS-like (COL) genes play an important role in the photoperiodic flowering pathway. However, the functional roles of the CO/COL...  相似文献   

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SAP1-1 and SAP1-2 were isolated from the male reproductive buds of willow (Salix discolor, clone S365). SAP1-1 differs from SAP1-2 based on a few nucleotide substitutions, but the sizes of their full-length cDNAs are identical. The deduced amino acid sequences of SAP1-1 and SAP1-2 were 98% similar and contain the same C-terminal amino acid motif “GYGA” like that of PTAP1-2 from Populus trichocarpa. The expression patterns of SAP1 in various parts of the male reproductive buds of S. discolor implicate this gene in the formation of the inflorescence meristems, bracts, and floral meristems. To characterize the functions of SAP1, we assessed Arabidopsis thaliana transformed with 35S∷SAP1-1. A total of 52 transgenic T1 lines were obtained, and a 3:1 segregation ratio was obtained in the T2 generation of each line. In the T3 generation, five homozygous transgenic lines were obtained, which were used for further analysis. Screening of transgenic lines was greatly facilitated by the detection of GFP expression starting with germinating seeds. Phenotypes of the homozygous transgenic lines included early flowering, conversion of inflorescence branches to solitary flowers, formation of terminal flowers, and formation of flowers with greater number of petals, stamens, and pistils. Northern analysis showed similar expression levels in all five lines. This study provides the first functional analysis of an APETALA1 (AP1)/SQUAMOSA (SQUA) homolog from a dioecious species and suggests that SAP1 is a homolog of the AP1/SQUA gene.  相似文献   

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The feasibility of using linkage disequilbrium (LD) to fine-map loci underlying natural variation in Arabidopsis thaliana was investigated by looking for associations between flowering time and marker polymorphism in the genomic regions containing two candidate genes, FRI and FLC, both of which are known to contribute to natural variation in flowering. A sample of 196 accessions was used, and polymorphism was assessed by sequencing a total of 17 roughly 500-bp fragments. Using a novel Bayesian algorithm based on haplotype similarity, we demonstrate that LD could have been used to fine-map the FRI gene to a roughly 30-kb region and to identify two common loss-of-function alleles. Interestingly, because of genetic heterogeneity, simple single-marker associations would not have been able to map FRI with nearly the same precision. No clear evidence for previously unknown alleles at either locus was found, but the effect of population structure in causing false positives was evident.  相似文献   

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The levels of beta-amylase activity and of the mRNA for beta-amylase in rosette leaves of Arabidopsis thaliana (L.) Heynh. increased significantly, with the concomitant accumulation of starch, when whole plants or excised mature leaves were supplied with sucrose. A supply of glucose or fructose, but not of mannitol or sorbitol, to plants also induced the expression of the gene for beta-amylase, and the induction occurred not only in rosette leaves but also in roots, stems, and bracts. These results suggest that the gene for beta-amylase of Arabidopsis is subject to regulation by a carbohydrate metabolic signal, and expression of the gene in various tissues may be regulated by the carbon partitioning and sink-source interactions in the whole plant. The sugar-inducible expression of the gene in Arabidopsis was severely repressed in the absence of light. The sugar-inducible expression in the light was not inhibited by 3(3,4-dichlorophenyl)-1,1-dimethylurea or by chloramphenicol, but it was inhibited by cycloheximide. These results suggest that a light-induced signal and de novo synthesis of proteins in the cytoplasm are involved in the regulation. A fusion gene composed of the 5' upstream region of the gene for beta-amylase from Arabidopsis and the coding sequence of beta-glucuronidase showed the sugar-inducible expression in a light-dependent manner in rosette leaves of transgenic Arabidopsis.  相似文献   

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