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1.
In this study, using the API-ZYM system, we have reported the enzyme profile of 42 soil strains and 2 clinical strains of
Nocardia asteroides isolated locally. Of the 19 enzymes tested, only 7 were demonstrable in over 90% of the soil isolates.
These included alkaline phosphatase, esterase lipase, leucine arylamidase, acid phosphatase, phosphohydrolase, α-glucosidase
and β-glucosidase. In addition, β-galactosidase activity was demonstrated in all the strains by the O-nitrophenyl-β-D-galactopyranoside
(ONPG) test. The enzymes which were not demonstrable in >95% of the strains included valine arylamidase, cystine arylamidase,
trypsin, chymotrypsin, α-galactosidase, β-glucoronidase, N-acetyl-β-glucosaminidase, α-mannosidase and α-fucosidase. With
the exception of valine arylamidase, which was lacking in all but one isolate, the enzyme profiles of the soil isolates were
comparable with the clinical isolates of N. asteroides reported in previous studies. The reasons for this difference in the
two sets of isolates is not clear. The study reinforces the view that specific differences in the enzymatic profiles of Nocardia
species could be used for their rapid identification. However, more extensive studies are needed to establish the reproducibility
of this method. To the best of our knowledge, this is the first study of the enzymatic profile of soil isolates of N. asteroides
originating from a single geographic region.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
Activities of 19 enzymes were tested by the API ZYM system in 13 strains ofBorrelia burgdorferi sensu lato (B. burgdorferi sensu stricto,B. afzelii, B. garinii, B. lusitaniae, B. valaisiana) grown in liquid BSK-H medium supplemented with rabbit serum. All strains produced acid phosphatase, esterase (C4), esterase-lipase
(C8), leucine arylamidase and naphthol-AS-BI-phosphohydrolase. Nine strains also produced alkaline phosphatase, and three
strains produced α-glucosidase. The API ZYM system probably cannot be used for differentiation betweenB. burgdorferi sensu lato genomospecies. 相似文献
3.
García-Martos P Marín P Hernández-Molina JM García-Agudo L Aoufi S Mira J 《Mycopathologia》2001,150(1):1-4
The extracellular enzymatic activity of 36 strains of yeast belonging to 11 species of the genus Cryptococcus, has been investigated, using the API-ZYM (BioMérieux, France) commercial system, with the objective of determining the differences
in the enzymatic profiles of the various species. The strains studied were : 9 of C. neoformans, 7 of C. albidus, 6 of C. laurentii, 5 of C. uniguttulatus, 3 of C. humicolus, and 1 each of C. ater, C. curvatus, C. dimennae, C. hungaricus, C. infirmo-miniatus and C. magnus. All the strains showed enzymatic activity with positivity to Phosphatase alkaline, Esterase lipase C8, Leucine arylamidase,
Phosphatase acid and Naphthol-AS-BI-phosphohydrolase, and negativity to Lipase C14, Trypsin, Chemotrypsin, β-galactosidase,
β-glucuronidase and α-manosidase. Variable enzymatic activity was shown to Esterase C4, Valine arylamidase, Cystine arylamidase,
α-galactosidase,α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminidase and α-fucosidase. This allowed 11 separate enzymatic
patterns to be established. The species C. neoformans and C. laurentii each presented two distinct patterns; C. uniguttulatus, C. hungaricus andC. magnus shared the same pattern; C. albidus, C. ater, C. curvatus,C. dimennae, C. humicolus and C. infirmo-miniatus presented an individual enzymatic pattern. The results obtained suggest that the API-ZYM system could be useful for the identification
of species of the genus Cryptococcus and for the differentiation of the enzymotypes for epidemiological purposes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
4.
A cell line designated as ADG was established from an abalone digestive gland using ERDF medium supplemented with 8% fetal
bovine serum (FBS), 8% abalone hemolymph, and high concentrations of NaCl, KCl, MgCl2, MgSO4, and CaCl2. ADG cells proliferated better in protein-free medium than in FBS-supplemented medium. Among 9 kinds of media examined, ERDF
medium was shown to be optimal for cell growth. ADG cells secreted 13 different kinds of glycosidases in protein-free medium:
α-L-fucosidase, β-L-fucosidase, α-D-galactosidase, β-D-galactosidase, N-acetyl-α-D-galactosaminidase, N-acetyl-β-D-galactosaminidase,
α-D-glucosidase, β-D-glucosidase, N-acetyl-α-D-glucosaminidase, N-acetyl-β-D-glucosaminidase, α-D-mannosidase, β-D-mannosidase,
β-D-xylosidase, and 1-3 xylanase. When ADG cells were cultured in Grace’s insect cell medium, the activity of some secreted
glycosidases increased 25-fold to 65-fold per cell as compared with control cells cultured in ERDF medium. ADG - abalone digestive
gland; ERDF - enriched RDF; FBS - fetal bovine serum; L-15 - Leibovitz’s L-15 media; DME - Dulbecco’s modified Eagle medium;
F-12 - nutrient mixture (Ham); LDF - L-15; DME: F-12 = 10 : 7 : 3; MEM - minimum essential medium; RPMI - RPMI medium 1640;
199 - media 199; GIC - Grace’s insect cell medium; pNP -p -nitrophenol.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
5.
The determination of the enzymatic activity of the yeasts has been applied to the identification of species, specially that ofCandida albicans. In order to know its usefulness in species of clinical interest, we have tested the commercial system API ZYM (Bio Mérieux) on 500 isolated strains of different organic samples, belonging to eight genera and twenty species. All the strains showed positivity to Phosphatase alcaline, Esterase (C4), Esterase lipase (C8), Leucine arylamidase and Phosphatase acid, and negativity to Lipase (C14), Trypsin, Chymotrypsin, -galactosidase, -glucoronidase, -manosidase and -fucosidase. Fourteen enzymatic activity patterns were obtained considering the substrates with variable results for the whole of the strains: Valine arylamidase, Cystine arylamidase, Naphthol-AS-BI-phosphohydrolase, -galactosidase, -glucosidase, -glucosidase and N-acetyl--glucosaminidase. In the majority of the species, the enzymatic profile did not have very specific results since it is usually shared by more than one species.C. albicans is that which presents the greatest number of enzymatic variations, some of these are similar to those of other common clinical species, such asCandida krusei, Candida parapsilosis andCandida tropicalis. This system is proposed as a rapid method for identification and as an epidemiological marker of medically important yeasts.Abbreviations AGL
-glucosidase
- BGA
-galactosidase
- BGL
-glucosidase
- CAA
Cystine arylamidase
- NAG
N.Acetyl--glucosaminidase
- PHO
Naphthol-AS-BI-phosphohydrolase
- VAA
Valine arylamidase 相似文献
6.
I. D. Georgieva 《Biologia Plantarum》1991,33(5):337-344
Changes in the activity and localization of nonspecific esterase, acid phosphatase, α-galactosidase and β-glucosidase inL. regale pistils after pollination with μ-irradiated pollen were studied. In the embryo sac and in the ovule reduction of AS-esterase
and α-galactosidase and, at the same time, enhancement of α-esterase, acid phosphatase and β-glucosidase activities were observed.
The changes in hydrolytic enzyme activities are discussed as manifestations of lethal factors resulting from structural disturbances
of DNA in the generative nucleus and in sperms caused by irradiation. 相似文献
7.
H. Kobayashi Hiroko Oyamada Naoko Iwadate Hiromi Suzuki Hideko Mitobe Kaori Takahashi Nobuyuki Shibata Shigeo Suzuki Yoshio Okawa 《Archives of microbiology》1998,169(3):188-194
A mannan of Candida glabrata IFO 0622 digested by Arthrobacter exo-α-mannosidase and a β-1,2-linked mannobiose obtained from the parent mannan by acid treatment was analyzed using 13C nuclear magnetic resonance spectroscopy. The results show that the β-1,2-linked mannobiosyl residue is esterified to a phosphate
group through position C-1 in the α-configuration, Manβ1– 2Manα1–HPO3–. The results of immunochemical assays of these mannans using the commercial antigenic factor sera of the genus Candida (Candida Check, Iatron) indicate that the main recognition site of serum no. 6 in this kit is the mannotetraosyl side-chain
Manβ1–2Manα1– 2Manα1–2Man in C. glabrata mannan and also suggest that the phosphate-containing unit (such as Manβ1– 2Manα1–HPO3– in this mannan) behaves as one of the antigenic determinants of serum no. 6, but not of serum no. 5. Therefore, the present
and previous findings indicate that serum no. 5 recognizes relatively longer β-1,2-linked oligomannosyl side-chains, Manβ1–[2Manβ1–]n 2Man (n = 1–6), attached to the phosphate groups previously observed in the cell wall mannans of Candida albicans, Candida stellatoidea, and Candida tropicalis.
Received: 18 March 1997 / Accepted: 16 September 1997 相似文献
8.
The leucocytes of three anguillid eels were studied using enzyme cytochemistry. Leucocytes were stained for peroxidase, alkaline phosphatase, acid phosphatase, aryl sulphatase, β-glucuronidase, N-acetyl-β-glucosaminidase, β-galactosidase, lysozyme, a variety of non-specific esterases, chloroacetate esterase and two proteases. All cells were negative for aryl sulphatase, β-glucuronidase, N-acetyl-β-glucosaminidase, and β-galactosidase. Very few neutrophils, thought to be mature, and all eosinophils contained peroxidase-positive granules, and some monocytes showed very weak peroxidase staining. All leucocytes lacked alkaline phosphatase, but all cells except lymphocytes and thrombocytes of A. dieffenbachii contained acid phosphatase. Neutrophil acid phosphatase released into phagosomes was associated with Escherischia coli bacteriolysis. Neutrophils also secrete lysozyme and, with monocytes, produce and secrete a variety of esterases. The possible interaction of lysozyme, acid phosphatase and esterases in bacteriolysis is discussed. 相似文献
9.
Nashwa M. Sallam A. A. Abd Elrazik M. Hassan E. Koch 《Archives Of Phytopathology And Plant Protection》2013,46(10):957-961
The APIZYM system of detection of enzymes was proven to be useful in the differentiation of 15 European and Egyptian isolates of S. cepivorum, the incitant of onion white rot. The tested isolates produced alkaline phosphatase, esterase (c4), esterase lipase (c8), leucine arylamidase, valinearylamidase, trypsine, α-chymatrypsin, acid phosphatase, naphthol-AS-B1-phosphohydrolase, ß-galactosidase, ß-glucutronidase, α-glucosidase, ß-glucosidase and N-acetyl-ß-glucosaminidase and did not produced lipase (c14), crystine arylamidase, trypsine, ß-glucutronidase, α-mannosidase and α-fucosidase. According to enzyme activity, isolates can be divided into four groups (G). The differences between groups were in the activity of the enzymes α-chymotrypsin and α-glucosidase. The tested European isolates and the Egyptian isolates No.6 of the pathogen were in G1 and G2; however the rest of the Egyptian isolates were in G3 and G4. 相似文献
10.
The enzymatic activity of 70 feline and canineMicrosporum canis isolates was determined by the Api-Zym® test. The liquid phase of cultures, inoculated into Tryptic Soy Broth, was used to examine 19 enzymes. Considerable differences were observed among the extracellular enzymatic patterns. All the isolates produced alkaline phosphatase and beta-glucosidase, while lipase (C14), trypsin, chymotrypsin, beta-glucuronidase, and alpha-fucosidase activity was never revealed. Esterase (C4) activity was present in 57 samples (81%), esterase lipase (C8) in 31 (44%), leucine arylamidase in 35 (50%), valine arylamidase and cystine arylamidase in 7 (10%), acid phosphatase in 64 (91%), naphthol-AS-BI-phosphohydrolase in 60 (86%), alpha-galactosidase in 5 (7%), beta-galactosidase in 6 (8%), alpha-glucosidase in 25 (36%), N-acetyl-beta-glucosaminidase in 41 (58%), and alpha-mannosidase in 51 (73%). The beta-galactosidase activity ofM. canis has not been reported previously. Remarkable variations of intensity for each enzymatic activity were also detected. It is believed that these results could provide basic data for further investigations on the pathogenic role of enzymes secreted byM. canis. 相似文献
11.
Jon J. Amberg Nathan R. Jensen Richard A. Erickson Blake Sauey Craig Jackson 《Ichthyological Research》2018,65(2):245-251
Typically, studies in digestive physiology in fish focus on a few enzymes and provide insight into the specific processes of the enzyme in a targeted species. Comparative studies assessing a wide number of digestive enzymes on fishes that compete for food resources are lacking, especially in the context of an introduced species. It is generally thought that the invasive silver carp (SVC; Hypophthalmichthys molitrix) directly compete for food resources with the native gizzard shad (GZS; Dorosoma cepedianum) in waters where they coexist. We compared 19 digestive enzymes between SVC and GZS throughout a year and in two rivers in the Midwestern United States: Illinois River and Wabash River. All digestive enzymes analyzed were detected in both SVC and GZS in both rivers. However, the profiles of the digestive enzymes varied by species. Alkaline phosphatase, valine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N-acetyl-β-glucosaminidase were all much higher in SVC than in GZS. Differences between digestive enzyme profiles were also observed between rivers and months. This study demonstrates the utility of using an ecological approach to compare physiological features in fishes. 相似文献
12.
Xiao-Jing Wang Ying-Feng Liu Qing-Yu Wang Morito Tsuruoka Kazumasa Ohta Sheng-Xi Wu Masashi Yakushiji Takashi Inoue 《Cell and tissue research》2010,340(2):347-355
Tobacco smoking is the main risk factor associated with chronic periodontitis, but the mechanisms that underlie this relationship
are largely unknown. Recent reports proposed that nicotine plays an important role in tobacco-related morbidity by acting
through the nicotinic acetylcholine receptors (nAChRs) expressed by non-neuronal cells. The aim of this study was to investigate
whether α7 nAChR was expressed in periodontal tissues and whether it functions by regulating IL-1β in the process of periodontitis.
In vitro, human periodontal ligament (PDL) cells were cultured with 10−12 M of nicotine and/or 10−9 M of alpha-bungarotoxin (α-Btx), a α7 nAChR antagonist. The expression of α7 nAChR and IL-1β in PDL cells and the effects
of nicotine/α-Btx administration on their expression were explored. In vivo, an experimental periodontitis rat model was established,
and the effects of nicotine/α-Btx administration on expression of α7 nAChR and development of periodontitis were evaluated.
We found that α7 nAChR was present in human PDL cells and rat periodontal tissues. The expressions of α7 nAChR and IL-1β were
significantly increased by nicotine administration, whereas α-Btx treatment partially suppressed these effects. This study
was the first to demonstrate the functional expression of α7 nAChR in human PDL cells and rat periodontal tissues. Our results
may be pertinent to a better understanding of the relationships among smoking, nicotine, and periodontitis. 相似文献
13.
Soussilane P Soussillane P D'Alessio C Paccalet T Fitchette AC Parodi AJ Williamson R Plasson C Faye L Gomord V 《Glycoconjugate journal》2009,26(5):609-607
Glucosidase II, one of the early N-glycan processing enzymes and a major player in the glycoprotein folding quality control, has been described as a soluble
heterodimer composed of α and β subunits. Here we present the first characterization of a plant glucosidase II α subunit at
the molecular level. Expression of the Arabidopsis α subunit restored N-glycan maturation capacity in Schizosaccharomyces pombe α− or αβ−deficient mutants, but with a lower efficiency in the last case. Inactivation of the α subunit in a temperature
sensitive Arabidopsis mutant blocked N-glycan processing after a first trimming by glucosidase I and strongly affected seedling development.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Cecilia D’Alessio and Thomas Paccalet have equal contributions to this work
An erratum to this article can be found at 相似文献
14.
Summary In this study, the variety of sugar residues in the gut glycoconjugates of Triturus carnifex (Amphibia, Caudata) are investigated by carbohydrate conventional histochemistry and lectin histochemistry. The oesophageal
surface mucous cells contained acidic glycoconjugates, with residues of GalNAc, Gal β1,3 GalNAc and (GlcNAc β1,4)
n
oligomers. The gastric surface cells mainly produced neutral glycoproteins with residues of fucose, Gal β1-3 GalNAc, Gal-αGal,
and (GlcNAc β1,4)
n
oligomers in N- and O-linked glycans, as the glandular mucous neck cells, with residues of mannose/glucose, GalNAc, Gal β1,3
GalNAc, (GlcNAc β1,4)
n
oligomers and fucose linked α1,6 or terminal α1,3 or α1,4 in O-linked glycans. The oxynticopeptic tubulo-vesicular system
contained neutral glycoproteins with N- and O-linked glycans with residues of Gal-αGal, Gal β1-3 GalNAc and (GlcNAc β1,4)
n
oligomers; Fuc linked α1,2 to Gal, α1,3 to GlcNAc in (poly)lactosamine chains and α1,6 to GlcNAc in N-linked glycans. Most
of these glycoproteins probably corresponds to the H+K+-ATPase β-subunit. The intestinal goblet cells contained acidic glycoconjugates, with residues of GalNAc, mannose/ glucose,
(GlcNAc β1,4)
n
oligomers and fucose linked α1,2 to Gal in O-linked oligosaccharides. The different composition of the mucus in the digestive
tracts may be correlated with its different functions. In fact the presence of abundant sulphation of glycoconjugates, mainly
in the oesophagus and intestine, probably confers resistance to bacterial enzymatic degradation of the mucus barrier. 相似文献
15.
N. V. Galayko I. A. Tolmacheva V. V. Grishko L. V. Volkova E. N. Perevozchikova S. A. Pestereva 《Russian Journal of Bioorganic Chemistry》2010,36(4):516-521
Novel hydrazones of the lupane and 19β,28-epoxy-18α-oleanane types have been synthesized via the interaction of 2,3-secotriterpenic
aldehydonitriles with substituted hydrazines. As a result of the investigation of the antiviral activity of 2,3-secotriterpenic
hydrazones against the Indiana strain of the vesicular stomatitis virus on two models of mammalian cell line infection, the
acetylhydrazone of 1-cyano-2,3-seco-19β,28-epoxy-18α-olean-3-al has been found to have a high prophylactic activity of 0.00016
μg/ml to the vesicular stomatitis virus and to inhibit virus reproduction in primarily infected cells in a 0.21-μg/ml concentration. 相似文献
16.
Peroxidase, alkaline phosphatase, acid phosphatase, β-glucuronidase, α-naphthyl acetate esterase (ANAE), α-naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, acetyl-L-tyrosine-α-naphthyl esterase (ATNE), tosyl-L-lysine-α-naphthyl esterase (TLNE) and periodic acid-Schiff (PAS) were studied in 17 species of elasmobranchs in which granulocytes had previously been identified at the ultrastructural level.
Eosinophils, eosinophilic and neutrophilic granulocytes contained variable acid phosphatase, esterases and PAS, but they were strongest in neutrophilic granulocytes; particularly ANAE. Esterases were released into surrounding plasma and therefore probably function as ectoenzymes. In eosinophils and some neutrophilic granulocytes there were indications of weak peroxidase, but this could not be conclusively demonstrated. Alkaline phosphatase was diffuse between granules in some eosinophils of Pavoraja , and (β-glucuronidase was diffuse in neutrophilic granulocytes of Etmopterus baxteri , otherwise granulocytes lacked these enzymes. Neutrophilic granulocytes stained moderately to strongly for ATNE and weakly and inconsistently for TLNE in Squalus acanthias and Dalatias licha . with a similar reaction in granular lymphocytoid and thrombocytoid cells of Galeorhinus ausiralis and Raja nasuta . The enzyme composition of these granulocytes is discussed. 相似文献
Eosinophils, eosinophilic and neutrophilic granulocytes contained variable acid phosphatase, esterases and PAS, but they were strongest in neutrophilic granulocytes; particularly ANAE. Esterases were released into surrounding plasma and therefore probably function as ectoenzymes. In eosinophils and some neutrophilic granulocytes there were indications of weak peroxidase, but this could not be conclusively demonstrated. Alkaline phosphatase was diffuse between granules in some eosinophils of Pavoraja , and (β-glucuronidase was diffuse in neutrophilic granulocytes of Etmopterus baxteri , otherwise granulocytes lacked these enzymes. Neutrophilic granulocytes stained moderately to strongly for ATNE and weakly and inconsistently for TLNE in Squalus acanthias and Dalatias licha . with a similar reaction in granular lymphocytoid and thrombocytoid cells of Galeorhinus ausiralis and Raja nasuta . The enzyme composition of these granulocytes is discussed. 相似文献
17.
J. R. Poortmans E. Blommaert M. Baptista M. E. De Broe E. J. Nouwen 《European journal of applied physiology and occupational physiology》1997,76(1):88-91
Post-exercise proteinuria is a common phenomenon in healthy subjects. Previous studies have used albumin (Alb) and β2-microglobulin (β2-m) molecules as representatives of high- and low-molecular-weight proteins. Recently, more specific markers of the human
kidney proximal tubule have been used to identify the precise site of alterations. Active male subjects underwent two strenuous
runs, one 400-m run and one 3000-m run. Urine was collected from the subjects before and after each event. Total protein (TP),
Alb, α1-microglobulin (α1-m), β2-m, intestinal alkaline phosphatase (IAP), tissue-nonspecific alkaline phosphatase (TNAP) and N-acetyl-β-d-glucosaminidase (NAG) were determined for each sample. The short-distance run (400 m) resulted in the largest increases (P ≤ 0.05) in TP (31-fold), Alb (100-fold) and β2-m (164-fold) as compared to the long-distance run (3000-m). The α1-m excretion rates were increased to a lesser extent by the exercises. The IAP activity was slightly increased (+90%) by the
400-m run while the TNAP and NAG activities showed a 6.8-fold and a 3.6-fold increase, respectively, after this event. Smaller
increases were recorded for the long-distance run (P = 0.05). To conclude, the present investigation showed that: (1) post-exercise proteinuria is related to the absolute intensity
of exercise; (2) the impairment of protein reabsorption is revealed better by changes in Alb and β2-m; (3) changes in TNAP and NAG activities could reveal biochemical modifications that occur in the proximal tubule, particularly
at the S1-S2 segment.
Accepted: 31 January 1997 相似文献
18.
Dolores Solis Juan J Calvete Libia Sanz Christiane Hettel Manfred Raida Teresa Diaz-Maurino Edda Topfer-Petersen 《Glycoconjugate journal》1997,14(2):275-280
Lectin mapping, carbohydrate analysis and electrospray mass spectrometry of boar seminal plasma PSP-II glycoforms show that
its single N-glycosylation site displays a repertoire of carbohydrate structures consisting of the basic pentasaccharide core
Manα 1–6[Manα 1–3]Manβ1-4GlcNAcβ1-4GlcNAc with a fucosyl residue α1-6-linked to the innermost N-acetylglucosamine residue. Other glycoforms display fucosylated hybrid-type or monoantennary complex-type chains, some of
which contain α2-6-linked sialic acid. N-acetylgalactosamine, possibly in Galβ1-3GalNAc sequence, is present in most of the PSP-II glycoforms. Abbreviations: PSP-I and PSP-II, porcine seminal plasma proteins
I and II; PNGaseF, peptide-N4-(N-acetyl-β-D-glucosaminyl) asparagine amidase (EC 3.5.1.52) from Flavobacterium meningosepticum;
ConA, Cannavalia ensiformis (jack bean) agglutinin; GNA, Galanthus nivalis (snowdrop) agglutin; SNA, Sambucus nigra (elderberry)
agglutinin; MAA, Maackia amurensis (maakia) agglutinin; PNA, Arachis hypogaea (peanut) agglutinin; DSA, Datura stramonium
(jimson weed) agglutinin; AAA, Aleuria aurantia agglutinin
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
19.
Manuela Oliveira M. Isabel Amorim Elsa Ferreira Luís Delgado Ilda Abreu 《Applied microbiology and biotechnology》2010,86(4):1171-1181
The aim of this work was to identify the main allergy-related Ascomycetes fungal spores present in the atmosphere of Porto,
using different and complementary techniques. The atmospheric sampling, performed in the atmosphere of Porto (Portugal) from
August 2006 to July 2008, indicated Cladosporium, Penicillium, Aspergillus and Alternaria as the main fungal spore taxa. Alternaria and Cladosporium peaks were registered during summer. Aspergillus and Penicillium highest values were registered from late winter to early spring. Additionally, the Andersen sampler allowed the culture and
isolation of the collected viable spores subsequently used for different identification approaches. The internal-transcribed
spacer region of the nuclear ribosomal repeat unit sequences of airborne Ascomycetes fungi isolates revealed 11 taxonomically
related fungal species. Among the identified taxa, Penicillum and Aspergillus presented the highest diversity, while only one species of Cladosporium and Alternaria, respectively, were identified. All selected fungal spore taxa possessed phosphatase, esterase, leucine arylamidase and β-glucosidase
enzymatic activity, while none had lipase, cystine arylamidase, trypsin or β-glucuronidase activity. The association between
the spore cell wall morphology, DNA-based techniques and enzymatic activity approaches allowed a more reliable identification
procedure of the airborne Ascomycota fungal spores. 相似文献
20.
The effect of phospholipase A2 (PLA2) inhibitor, quinacrine, on the activity of hydrolytic enzymes in Tetrahymena pyriformis homogenate, was investigated. The activity of all of the enzymes studied (acid phosphatase, N-acetyl-beta-glusosaminidase, glucosidase, galactosidase and esterase) was significantly reduced in the presence of quinacrine. Since there are no data on the inhibitory effect of PLA2 and PLA2 influenced metabolic pathways to the hydrolytic enzymes, the direct effect of quinacrine on the hydrolytic enzymes (of Tetrahymena) can be supposed. This is supported by the fact that the other PLA2 inhibitor, 4-bromophenacyl bromide, did not influence phosphatase activity. 相似文献